{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T16:03:20Z","timestamp":1774886600598,"version":"3.50.1"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Fachagentur Nachwachsende Rohstoffe e.V. (FNR)","award":["22029812A"],"award-info":[{"award-number":["22029812A"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2017,9]]},"DOI":"10.1016\/j.jbiotec.2017.07.004","type":"journal-article","created":{"date-parts":[[2017,7,8]],"date-time":"2017-07-08T21:03:52Z","timestamp":1499547832000},"page":"33-40","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Development of a single-cell GlxR-based cAMP biosensor for Corynebacterium glutamicum"],"prefix":"10.1016","volume":"258","author":[{"given":"Julia","family":"Schulte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meike","family":"Baumgart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Bott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiotec.2017.07.004_bib0005","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/349694a0","article-title":"Fluorescence ratio imaging of cyclic AMP in single cells","volume":"349","author":"Adams","year":"1991","journal-title":"Nature"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0010","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1111\/j.1462-5822.2010.01562.x","article-title":"Cyclic AMP signalling in mycobacteria: redirecting the conversation with a common currency","volume":"13","author":"Bai","year":"2011","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0015","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/1741-7007-11-122","article-title":"IpsA, a novel LacI-type regulator, is required for inositol-derived lipid formation in Corynebacteria and Mycobacteria","volume":"11","author":"Baumgart","year":"2013","journal-title":"BMC Biol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0020","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.copbio.2011.11.012","article-title":"Bio-based production of chemicals, materials and fuels\u2014Corynebacterium glutamicum as versatile cell factory","volume":"23","author":"Becker","year":"2012","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0025","doi-asserted-by":"crossref","first-page":"3300","DOI":"10.1128\/AEM.02972-10","article-title":"Corynebacterium glutamicum tailored for efficient isobutanol production","volume":"77","author":"Blombach","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0030","doi-asserted-by":"crossref","first-page":"7855","DOI":"10.1073\/pnas.1401917111","article-title":"Family of prokaryote cyclic nucleotide-modulated ion channels","volume":"111","author":"Brams","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0035","series-title":"Corynebacteria: Genomics and Molecular Biology","author":"Burkovski","year":"2008"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0040","series-title":"Corynebacterium glutamicum\u2212From Systems Biology to Biotechnological Applications","author":"Burkovski","year":"2015"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0045","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1007\/s00253-009-2066-9","article-title":"Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032","volume":"85","author":"Cha","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0050","series-title":"Handbook of Corynebacterium glutamicum","author":"Eggeling","year":"2005"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0055","doi-asserted-by":"crossref","first-page":"3387","DOI":"10.1007\/s00253-015-6508-2","article-title":"A giant market and a powerful metabolism: l-lysine provided by Corynebacterium glutamicum","volume":"99","author":"Eggeling","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0060","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.copbio.2014.12.021","article-title":"Novel screening methods-biosensors","volume":"35","author":"Eggeling","year":"2015","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0065","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0022-2836(83)80284-8","article-title":"Studies on transformation of Escherichia coli with plasmids","volume":"166","author":"Hanahan","year":"1983","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0070","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1111\/j.1751-7915.2012.00360.x","article-title":"Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum","volume":"6","author":"Hentschel","year":"2013","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0075","doi-asserted-by":"crossref","first-page":"e93587","DOI":"10.1371\/journal.pone.0093587","article-title":"Role of Corynebacterium glutamicum sprA encoding a serine protease in glxR-mediated global gene regulation","volume":"9","author":"Hong","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0080","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1023\/A:1008968419217","article-title":"Construction and application of new Corynebacterium glutamicum vectors","volume":"13","author":"Jakoby","year":"1999","journal-title":"Biotechnol. Techn."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0085","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1007\/s00253-014-6223-4","article-title":"Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum","volume":"99","author":"Jojima","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0090","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/j.1574-6968.2008.01098.x","article-title":"Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR","volume":"281","author":"Jungwirth","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0095","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1099\/mic.0.062059-0","article-title":"High-resolution detection of DNA binding sites of the global transcriptional regulator GlxR in Corynebacterium glutamicum","volume":"159","author":"Jungwirth","year":"2013","journal-title":"Microbiology"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0100","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0168-1656(03)00154-8","article-title":"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l-aspartate-derived amino acids and vitamins","volume":"104","author":"Kalinowski","year":"2003","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0105","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.ymben.2016.06.003","article-title":"Construction of a Corynebacterium glutamicum platform strain for the production of stilbenes and (2S)-flavanones","volume":"38","author":"Kallscheuer","year":"2016","journal-title":"Metab. Eng."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0110","doi-asserted-by":"crossref","first-page":"5595","DOI":"10.1128\/jb.175.17.5595-5603.1993","article-title":"Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon","volume":"175","author":"Keilhauer","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0115","doi-asserted-by":"crossref","first-page":"3453","DOI":"10.1128\/JB.186.11.3453-3460.2004","article-title":"Identification and characterization of glxR, a gene involved in regulation of glyoxylate bypass in Corynebacterium glutamicum","volume":"186","author":"Kim","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0120","doi-asserted-by":"crossref","first-page":"193","DOI":"10.2323\/jgam.3.193","article-title":"Studies on amino acid fermentation. Part I. Production of l-glutamic acid by various microorganisms","volume":"3","author":"Kinoshita","year":"1957","journal-title":"J. Gen. Appl. Microbiol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0125","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.jbiotec.2009.08.005","article-title":"The GlxR regulon of the amino acid producer Corynebacterium glutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model","volume":"143","author":"Kohl","year":"2009","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0130","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.jbiotec.2008.05.011","article-title":"The GlxR regulon of the amino acid producer Corynebacterium glutamicum: in silico and in vitro detection of DNA binding sites of a global transcription regulator","volume":"135","author":"Kohl","year":"2008","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0135","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1146\/annurev.bi.62.070193.003533","article-title":"Transcriptional regulation by cAMP and its receptor protein","volume":"62","author":"Kolb","year":"1993","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0140","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1111\/1751-7915.12236","article-title":"A chromosomally encoded T7 RNA polymerase-dependent gene expression system for Corynebacterium glutamicum: construction and comparative evaluation at the single-cell level","volume":"8","author":"Kortmann","year":"2015","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0145","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1128\/JB.06064-11","article-title":"Arabitol metabolism of Corynebacterium glutamicum and its regulation by AtlR","volume":"194","author":"Laslo","year":"2012","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0150","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s00253-015-7090-3","article-title":"Transcription factor-based biosensors in biotechnology: current state and future prospects","volume":"100","author":"Mahr","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0155","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1101\/gr.10.2.204","article-title":"Functional classification of cNMP-binding proteins and nucleotide cyclases with implications for novel regulatory pathways in Mycobacterium tuberculosis","volume":"10","author":"McCue","year":"2000","journal-title":"Genome Res."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0160","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M113.463992","article-title":"Cyclic AMP-dependent protein lysine acylation in mycobacteria regulates fatty acid and propionate metabolism","volume":"288","author":"Nambi","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0165","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1007\/s002030100262","article-title":"Molecular analysis of the cytochrome bc1-aa3 branch of the Corynebacterium glutamicum respiratory chain containing an unusual diheme cytochrome c1","volume":"175","author":"Niebisch","year":"2001","journal-title":"Arch. Microbiol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0170","doi-asserted-by":"crossref","first-page":"196","DOI":"10.3389\/fphar.2015.00196","article-title":"Genetically-encoded tools for cAMP probing and modulation in living systems","volume":"6","author":"Paramonov","year":"2015","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0175","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/j.1574-6968.2009.01884.x","article-title":"Functional characterization of the glxR deletion mutant of Corynebacterium glutamicum ATCC 13032: involvement of GlxR in acetate metabolism and carbon catabolite repression","volume":"304","author":"Park","year":"2010","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0180","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3389\/fphar.2015.00164","article-title":"The genetically encoded tool set for investigating cAMP: more than the sum of its parts","volume":"6","author":"Patel","year":"2015","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0185","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1574-6968.2007.00793.x","article-title":"Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis","volume":"273","author":"Polen","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0190","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0195","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0378-1119(94)90324-7","article-title":"Small mobilizable multipurpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19\u2014s: selection of defined deletions in the chromosome of Corynebacterium glutamicum","volume":"145","author":"Sch\u00e4fer","year":"1994","journal-title":"Gene"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0200","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00253-011-3252-0","article-title":"Biotechnological production of polyamines by bacteria: recent achievements and future perspectives","volume":"91","author":"Schneider","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0205","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1111\/mmi.13574","article-title":"Identification of the cAMP phosphodiesterase CpdA as novel key player in cAMP-dependent regulation in Corynebacterium glutamicum","volume":"103","author":"Schulte","year":"2017","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0210","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.tim.2006.10.005","article-title":"New messages from old messengers: cAMP and mycobacteria","volume":"14","author":"Shenoy","year":"2006","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0215","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.4014\/jmb.1502.02053","article-title":"Identification of the regulators binding to the upstream region of glxR in Corynebacterium glutamicum","volume":"25","author":"Subhadra","year":"2015","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0220","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1146\/annurev.bi.59.070190.004543","article-title":"cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes","volume":"59","author":"Taylor","year":"1990","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0225","doi-asserted-by":"crossref","first-page":"e113265","DOI":"10.1371\/journal.pone.0113265","article-title":"The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP\/FNR family transcription factors","volume":"9","author":"Townsend","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0230","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1128\/JB.01425-08","article-title":"Involvement of the LuxR-type transcriptional regulator RamA in regulation of expression of the gapA gene, encoding glyceraldehyde-3-phosphate dehydrogenase of Corynebacterium glutamicum","volume":"191","author":"Toyoda","year":"2009","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0235","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.1128\/JB.00384-11","article-title":"Genome-wide identification of in vivo binding sites of GlxR, a cyclic AMP receptor protein-type regulator in Corynebacterium glutamicum","volume":"193","author":"Toyoda","year":"2011","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0240","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1146\/annurev.biochem.67.1.509","article-title":"The green fluorescent protein","volume":"67","author":"Tsien","year":"1998","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0245","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s12934-015-0216-6","article-title":"Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping","volume":"14","author":"Unthan","year":"2015","journal-title":"Microb. cell fact."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0250","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s11274-016-2060-1","article-title":"Updates on industrial production of amino acids using Corynebacterium glutamicum","volume":"32","author":"Wendisch","year":"2016","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0255","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/1751-7915.12013","article-title":"Bio-based production of organic acids with Corynebacterium glutamicum","volume":"6","author":"Wieschalka","year":"2013","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.jbiotec.2017.07.004_bib0260","series-title":"Microbiology Monographs","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-29857-8","article-title":"Corynebacterium glutamicum: Biology and Biotechnology","author":"Yukawa","year":"2013"}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016816561731516X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016816561731516X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T11:58:54Z","timestamp":1535630334000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016816561731516X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9]]},"references-count":52,"alternative-id":["S016816561731516X"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2017.07.004","relation":{},"ISSN":["0168-1656"],"issn-type":[{"value":"0168-1656","type":"print"}],"subject":[],"published":{"date-parts":[[2017,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a single-cell GlxR-based cAMP biosensor for Corynebacterium glutamicum","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2017.07.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}