{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:41:59Z","timestamp":1761745319439,"version":"3.44.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.jbiotec.2020.09.011","type":"journal-article","created":{"date-parts":[[2020,9,19]],"date-time":"2020-09-19T20:31:02Z","timestamp":1600547462000},"page":"389-394","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["Rapid sensing of clinically relevant glutamine concentrations in human serum with metabolically engineered E. coli-based cell-free protein synthesis"],"prefix":"10.1016","volume":"325","author":[{"given":"J. Porter","family":"Hunt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Jordan","family":"Barnett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehran","family":"Soltani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Andrew D.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4438-183X","authenticated-orcid":false,"given":"Bradley C.","family":"Bundy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiotec.2020.09.011_bib0005","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.3109\/10428194.2014.1003056","article-title":"Asparaginase pharmacokinetics and implications of therapeutic drug monitoring","volume":"56","author":"Asselin","year":"2015","journal-title":"Leuk. Lymphoma"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0010","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1002\/pbc.25299","article-title":"Clinical application of asparaginase activity levels following treatment with pegaspargase","volume":"62","author":"Bleyer","year":"2015","journal-title":"Pediatr. Blood Cancer"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0015","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1021\/bc9002844","article-title":"Site-specific incorporation of p-Propargyloxyphenylalanine in a cell-free environment for direct protein-Protein click conjugation","volume":"21","author":"Bundy","year":"2010","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0020","doi-asserted-by":"crossref","first-page":"806","DOI":"10.2337\/diacare.22.5.806","article-title":"Protein metabolism in insulin-treated gestational diabetes","volume":"22","author":"Butte","year":"1999","journal-title":"Diabetes Care"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0025","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.jbiotec.2007.07.951","article-title":"l-Methionine sulfoximine as a novel selection agent for genetic transformation of orchids","volume":"131","author":"Chai","year":"2007","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0030","doi-asserted-by":"crossref","first-page":"7010","DOI":"10.1038\/s41598-019-43431-z","article-title":"Serum amino acid profiles and risk of type 2 diabetes among Japanese adults in the Hitachi Health Study","volume":"9","author":"Chen","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0035","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1093\/chromsci\/bmu022","article-title":"Rapid determination of glutamine in human plasma by high-performance liquid chromatographic\u2013Tandem mass spectrometry and its application in pharmacokinetic studies","volume":"53","author":"Du","year":"2014","journal-title":"J. Chromatogr. Sci."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0040","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jprot.2014.03.025","article-title":"Metabolic fingerprint of gestational diabetes mellitus","volume":"103","author":"Dudzik","year":"2014","journal-title":"J. Proteomics"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0045","doi-asserted-by":"crossref","first-page":"36","DOI":"10.59566\/IJBS.2014.10036","article-title":"Some amino acids levels: glutamine,glutamate, and homocysteine, in plasma of children with chronic kidney disease","volume":"10","author":"Fadel","year":"2014","journal-title":"Int. J. Biomed. Sci."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0050","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1097\/MPH.0000000000001062","article-title":"Symptomatic hyperammonemia with Erwinia chrysanthemi\u2013derived asparaginase in pediatric leukemia patients","volume":"40","author":"Gossai","year":"2018","journal-title":"J. Pediatr. Hematol. Oncol."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0055","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1021\/acssynbio.9b00433","article-title":"Unlocking applications of cell-free biotechnology through enhanced shelf life and productivity of E. Coli extracts","volume":"9","author":"Gregorio","year":"2020","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0060","doi-asserted-by":"crossref","first-page":"4079","DOI":"10.1158\/1078-0432.CCR-18-3223","article-title":"Targeting glutamine metabolism and redox state for leukemia therapy","volume":"25","author":"Gregory","year":"2019","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0065","doi-asserted-by":"crossref","first-page":"3678","DOI":"10.1172\/JCI69600","article-title":"Glutamine and cancer: cell biology, physiology, and clinical opportunities","volume":"123","author":"Hensley","year":"2013","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0070","doi-asserted-by":"crossref","DOI":"10.1002\/biot.201900294","article-title":"Engineering cell-free protein synthesis for high-yield production and human serum activity assessment of asparaginase: toward on-demand treatment of acute lymphoblastic leukemia","volume":"15","author":"Hunt","year":"2020","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0075","doi-asserted-by":"crossref","first-page":"E163","DOI":"10.1152\/ajpendo.1999.276.1.E163","article-title":"The metabolic consequences of critical illness: acute effects on glutamine and protein metabolism","volume":"276","author":"Jackson","year":"1999","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0080","doi-asserted-by":"crossref","first-page":"9638","DOI":"10.1021\/acs.analchem.7b01956","article-title":"Complementary cell-free translational assay for quantification of amino acids","volume":"89","author":"Jang","year":"2017","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0085","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1021\/acs.analchem.8b05526","article-title":"Interfacing a personal glucose meter with cell-free protein synthesis for rapid analysis of amino acids","volume":"91","author":"Jang","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0090","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1023\/A:1005624811710","article-title":"Oxalate improves protein synthesis by enhancing ATP supply in a cell-free system derived from Escherichia coli","volume":"22","author":"Kim","year":"2000","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0095","doi-asserted-by":"crossref","first-page":"10499","DOI":"10.1073\/pnas.0502248102","article-title":"Structure of Mycobacterium tuberculosis glutamine synthetase in complex with a transition-state mimic provides functional insights","volume":"102","author":"Krajewski","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0100","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1097\/SHK.0000000000001138","article-title":"Cerebral multimodal monitoring in Sepsis: an experimental study","volume":"51","author":"Kurtz","year":"2019","journal-title":"Shock"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0105","series-title":"Heterogeneity of Cancer Metabolism. Springer International Publishing Ag","first-page":"13","article-title":"Glutamine metabolism in cancer","author":"Li","year":"2018"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0110","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1093\/oxfordjournals.jbchem.a135432","article-title":"Synergistic inhibition of phosphoenolpyruvate carboxylase by aspartate and 2-oxoglutarate in Brevibacterium flavum","volume":"98","author":"Mori","year":"1985","journal-title":"J. Biochem."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0115","doi-asserted-by":"crossref","DOI":"10.1186\/1744-859X-9-29","article-title":"Serum glutamine, set-shifting ability and anorexia nervosa","volume":"9","author":"Nakazato","year":"2010","journal-title":"Ann. Gen. Psychiatry"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0120","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1002\/bmc.1096","article-title":"An isocratic fluorescence HPLC assay for the monitoring of L-asparaginase activity and L-asparagine depletion in children receiving E. Coli L-asparaginase for the treatment of acute lymphoblastic leukaemia","volume":"23","author":"Nath","year":"2009","journal-title":"Biomed. Chromatogr."},{"year":"2020","series-title":"Clinical Trials Using Glutaminase Inhibitor CB-839","author":"National Cancer Institute","key":"10.1016\/j.jbiotec.2020.09.011_bib0125"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0130","doi-asserted-by":"crossref","DOI":"10.3390\/nu8020076","article-title":"Exogenous Glutamine in Respiratory Diseases: Myth or Reality?","volume":"8","author":"Oliveira","year":"2016","journal-title":"Nutrients"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0135","first-page":"1121","article-title":"Deamination of glutamine is a prerequisite for optimal asparagine deamination by asparaginases in vivo (CCG-1961)","volume":"24","author":"Panosyan","year":"2004","journal-title":"Anticancer Res."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0140","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.cell.2014.10.004","article-title":"Paper-based synthetic gene networks","volume":"159","author":"Pardee","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0145","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.cell.2016.04.059","article-title":"Rapid, low-cost detection of zika virus using programmable biomolecular components","volume":"165","author":"Pardee","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0150","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1038\/sj.bjc.6604019","article-title":"Purinergic regulation of angiogenesis by human breast carcinoma-secreted nucleoside diphosphate kinase","volume":"97","author":"Rumjahn","year":"2007","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0155","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1021\/acs.analchem.6b04034","article-title":"Cell-free protein synthesis approach to biosensing hTR\u03b2-Specific endocrine disruptors","volume":"89","author":"Salehi","year":"2017","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0160","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.taap.2018.02.016","article-title":"Biosensing estrogenic endocrine disruptors in human blood and urine: a RAPID cell-free protein synthesis approach","volume":"345","author":"Salehi","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0165","doi-asserted-by":"crossref","first-page":"6539","DOI":"10.1073\/pnas.1507228112","article-title":"MYC oncogene overexpression drives renal cell carcinoma in a mouse model through glutamine metabolism","volume":"112","author":"Shroff","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0170","doi-asserted-by":"crossref","first-page":"186","DOI":"10.2144\/000114158","article-title":"Lyophilized Escherichia coli-based cell-free systems for robust, high-density, long-term storage","volume":"56","author":"Smith","year":"2014","journal-title":"Biotechniques"},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0175","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1002\/btpr.2157","article-title":"Creating a completely \"Cell-free\" system for protein synthesis","volume":"31","author":"Smith","year":"2015","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0180","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.bej.2018.06.014","article-title":"Reengineering cell-free protein synthesis as a biosensor: biosensing with transcription, translation, and protein-folding","volume":"138","author":"Soltani","year":"2018","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0185","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cobme.2019.08.005","article-title":"Cell-free biosensors for biomedical applications","volume":"13","author":"Voyvodic","year":"2020","journal-title":"Curr. Opin. Biomed. Eng."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0190","doi-asserted-by":"crossref","first-page":"19646","DOI":"10.1073\/pnas.1907946116","article-title":"Mode-of-action profiling reveals glutamine synthetase as a collateral metabolic vulnerability of M. Tuberculosis to bedaquiline","volume":"116","author":"Wang","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0195","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1021\/acssynbio.7b00219","article-title":"A cell-free biosensor for detecting quorum sensing molecules in P. Aeruginosa-infected respiratory samples","volume":"6","author":"Wen","year":"2017","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0200","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.nbt.2019.07.004","article-title":"Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free therapeutic production","volume":"53","author":"Wilding","year":"2019","journal-title":"N. Biotechnol."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0205","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jbiotec.2014.10.039","article-title":"Enhanced enzyme stability through site-directed covalent immobilization","volume":"193","author":"Wu","year":"2015","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiotec.2020.09.011_bib0210","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jbiotec.2019.05.011","article-title":"Detection of inorganic ions and organic molecules with cell-free biosensing systems","volume":"300","author":"Zhang","year":"2019","journal-title":"J. Biotechnol."}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165620302479?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165620302479?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T12:56:30Z","timestamp":1759150590000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165620302479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":42,"alternative-id":["S0168165620302479"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2020.09.011","relation":{},"ISSN":["0168-1656"],"issn-type":[{"type":"print","value":"0168-1656"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid sensing of clinically relevant glutamine concentrations in human serum with metabolically engineered E. coli-based cell-free protein synthesis","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2020.09.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}