{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T19:35:03Z","timestamp":1779392103430,"version":"3.53.1"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"am","delay-in-days":323,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["JPELBCPI2015003"],"award-info":[{"award-number":["JPELBCPI2015003"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["JPELBCPI2017003"],"award-info":[{"award-number":["JPELBCPI2017003"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["JSYSZJ2019001"],"award-info":[{"award-number":["JSYSZJ2019001"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1016\/j.jbiotec.2022.09.011","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T03:31:05Z","timestamp":1663644665000},"page":"29-34","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["An acetate-independent pathway for isopropanol production via HMG-CoA in Escherichia coli"],"prefix":"10.1016","volume":"359","author":[{"given":"Jia","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengdie","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyi","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangqian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongbiao","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoxia","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiotec.2022.09.011_bib1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0378-1119(88)90440-4","article-title":"Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli","volume":"69","author":"Amann","year":"1988","journal-title":"Gene"},{"key":"10.1016\/j.jbiotec.2022.09.011_bib2","doi-asserted-by":"crossref","first-page":"6468","DOI":"10.1128\/jb.171.12.6468-6472.1989","article-title":"Nucleotide sequence and expression in Escherichia coli of the 3-hydroxy-3-methylglutaryl coenzyme A lyase gene of Pseudomonas mevalonii","volume":"171","author":"Anderson","year":"1989","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib3","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/978-1-59745-321-9_11","article-title":"The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12","volume":"416","author":"Baba","year":"2008","journal-title":"Methods Mol. Biol."},{"issue":"64","key":"10.1016\/j.jbiotec.2022.09.011_bib4","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1128\/AEM.64.3.1079-1085.1998","article-title":"Expression of Clostridium acetobutylicum ATCC 824 genes in Escherichia coli for acetone production and acetate detoxification","author":"Bermejo","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib5","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/j.1574-6968.2009.01624.x","article-title":"The Pseudomonas aeruginosa liuE gene encodes the 3-hydroxy-3-methylglutaryl coenzyme A lyase, involved in leucine and acyclic terpene catabolism","volume":"296","author":"Chavez-Aviles","year":"2009","journal-title":"FEMS Microbiol Lett."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib6","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/BF01040869","article-title":"Acetone-butanol-isopropanol production by Clostridium beijerinckii","volume":"8","author":"Chen","year":"1986","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib7","doi-asserted-by":"crossref","first-page":"7542","DOI":"10.1128\/AEM.71.11.7542-7547.2005","article-title":"Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron","volume":"71","author":"Chen","year":"2005","journal-title":"Appl. Environ. Microbiol"},{"key":"10.1016\/j.jbiotec.2022.09.011_bib8","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jbiotec.2022.09.011_bib9","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s10545-014-9704-9","article-title":"Ketone body metabolism and its defects","volume":"37","author":"Fukao","year":"2014","journal-title":"J. Inherit. Metab. Dis."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib10","doi-asserted-by":"crossref","first-page":"7814","DOI":"10.1128\/AEM.01140-07","article-title":"Engineered synthetic pathway for isopropanol production in Escherichia coli","volume":"73","author":"Hanai","year":"2007","journal-title":"Appl. Environ. Microbiol"},{"key":"10.1016\/j.jbiotec.2022.09.011_bib11","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1007\/s00253-007-1246-8","article-title":"Production of isopropanol by metabolically engineered Escherichia coli","volume":"77","author":"Jojima","year":"2008","journal-title":"Appl. Microbiol Biotechnol."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib12","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.ymben.2012.08.001","article-title":"A modified pathway for the production of acetone in Escherichia coli","volume":"15","author":"May","year":"2013","journal-title":"Metab. Eng."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib13","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0076-6879(00)24227-4","article-title":"Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase","volume":"324","author":"Miziorko","year":"2000","journal-title":"Methods Enzym."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib14","doi-asserted-by":"crossref","first-page":"11224","DOI":"10.1021\/bi00160a037","article-title":"Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase: characterization of the isolated recombinant protein and investigation of the enzyme's cation requirements","volume":"31","author":"Narasimhan","year":"1992","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbiotec.2022.09.011_bib15","doi-asserted-by":"crossref","first-page":"9930","DOI":"10.1021\/bi00031a015","article-title":"Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase: testing the function of the active site cysteine by site-directed mutagenesis","volume":"34","author":"Narasimhan","year":"1995","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbiotec.2022.09.011_bib16","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/0003-9861(77)90496-9","article-title":"Steady state kinetic mechanism of the Escherichia coli coenzyme A transferase","volume":"181","author":"Sramek","year":"1977","journal-title":"Arch. Biochem Biophys."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib17","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1128\/aem.55.2.323-329.1989","article-title":"Coenzyme A transferase from Clostridium acetobutylicum ATCC 824 and its role in the uptake of acids","volume":"55","author":"Wiesenborn","year":"1989","journal-title":"Appl. Environ. Microbiol"},{"issue":"296","key":"10.1016\/j.jbiotec.2022.09.011_bib18","article-title":"Efficient isopropanol biosynthesis by engineered Escherichia coli using biologically produced acetate from syngas fermentation","author":"Yang","year":"2020","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib19","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jbiotec.2013.11.009","article-title":"Engineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation","volume":"169","author":"Zhou","year":"2014","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib20","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.enzmictec.2014.10.005","article-title":"Geranyl diphosphate synthase: an important regulation point in balancing a recombinant monoterpene pathway in Escherichia coli","volume":"68","author":"Zhou","year":"2015","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.jbiotec.2022.09.011_bib21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jbiotec.2017.03.005","article-title":"Enhanced performance of the methylerythritol phosphate pathway by manipulation of redox reactions relevant to IspC, IspG, and IspH","volume":"248","author":"Zhou","year":"2017","journal-title":"J. Biotechnol."},{"issue":"9","key":"10.1016\/j.jbiotec.2022.09.011_bib22","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s13205-019-1867-5","article-title":"Knockout of acetoacetate degradation pathway gene atoDA enhances the toxicity tolerance of Escherichia coli to isopropanol and acetone","author":"Zhou","year":"2019","journal-title":"3 Biotech"},{"key":"10.1016\/j.jbiotec.2022.09.011_bib23","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1007\/s13205-020-02523-8","article-title":"A gas chromatography-flame ionization detection method for direct and rapid determination of small molecule volatile organic compounds in aqueous phase","volume":"10","author":"Zhou","year":"2020","journal-title":"3 Biotech"}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016816562200219X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016816562200219X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T12:59:23Z","timestamp":1759150763000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016816562200219X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":23,"alternative-id":["S016816562200219X"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2022.09.011","relation":{},"ISSN":["0168-1656"],"issn-type":[{"value":"0168-1656","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An acetate-independent pathway for isopropanol production via HMG-CoA in Escherichia coli","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2022.09.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}