{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T02:18:32Z","timestamp":1789957112752,"version":"4.0.1"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100008977","name":"Universit\u00e4t Ulm","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008977","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003542","name":"Ministerium f\u00fcr Wissenschaft, Forschung und Kunst Baden-W\u00fcrttemberg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004581","name":"Brandenburger Staatsministerium f\u00fcr Wissenschaft, Forschung und Kultur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004581","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2023,1]]},"DOI":"10.1016\/j.jbiotec.2022.11.015","type":"journal-article","created":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T12:25:49Z","timestamp":1670329549000},"page":"49-56","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["An efficient capture strategy for the purification of human adenovirus type 5 from cell lysates"],"prefix":"10.1016","volume":"361","author":[{"given":"Sandra","family":"Dietl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Kiefer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Binder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Walther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Sobek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Mizaikoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiotec.2022.11.015_bib1","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.3389\/fmicb.2016.01503","article-title":"Components of adenovirus genome packaging","volume":"7","author":"Ahi","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib2","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1038\/sj.gt.3301190","article-title":"An improved anion-exchange HPLC method for the detection and purification of adenoviral particles","volume":"7","author":"Blanche","year":"2000","journal-title":"Gene Ther."},{"issue":"1","key":"10.1016\/j.jbiotec.2022.11.015_bib3","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/s41392-021-00487-6","article-title":"Viral vector platforms within the gene therapy landscape","volume":"6","author":"Bulcha","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"issue":"S1","key":"10.1016\/j.jbiotec.2022.11.015_bib4","doi-asserted-by":"crossref","first-page":"S5","DOI":"10.1038\/sj.gt.3302611","article-title":"Chromatographic purification of recombinant adenoviral and adeno-associated viral vectors: methods and implications","volume":"12","author":"Burova","year":"2005","journal-title":"Gene Ther."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib5","series-title":"Virology: Principles and Applications","author":"Carter","year":"2013"},{"key":"10.1016\/j.jbiotec.2022.11.015_bib6","series-title":"Encyclopedia of analytical chemistry","first-page":"10815","article-title":"Interpretation of Infrared Spectra, a Practical Approach","author":"Coates","year":"2000"},{"issue":"24","key":"10.1016\/j.jbiotec.2022.11.015_bib7","doi-asserted-by":"crossref","first-page":"7692","DOI":"10.3390\/ma14247692","article-title":"Core-Shell imprinted particles for adenovirus binding","volume":"14","author":"Dietl","year":"2021","journal-title":"Materials"},{"key":"10.1016\/j.jbiotec.2022.11.015_bib8","series-title":"Principles of Virology","author":"Flint","year":"2015"},{"issue":"9","key":"10.1016\/j.jbiotec.2022.11.015_bib9","doi-asserted-by":"crossref","first-page":"5576","DOI":"10.1021\/acs.analchem.7b05148","article-title":"Enhanced selectivity by passivation: molecular imprints for viruses with exceptional binding properties","volume":"90","author":"Gast","year":"2018","journal-title":"Anal. Chem."},{"issue":"6","key":"10.1016\/j.jbiotec.2022.11.015_bib10","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1089\/hgtb.2019.172","article-title":"Nanoparticle tracking of adenovirus by light scattering and fluorescence detection","volume":"30","author":"Gast","year":"2019","journal-title":"Hum. Gene Ther. Methods"},{"issue":"4","key":"10.1016\/j.jbiotec.2022.11.015_bib11","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1021\/acs.analchem.9b04328","article-title":"Use of super-resolution optical microscopy to reveal direct virus binding at hybrid core\u2013shell matrixes","volume":"92","author":"Gast","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib12","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1089\/10430340260355338","article-title":"A new scalable method for the purification of recombinant adenovirus vectors","volume":"13","author":"Green","year":"2002","journal-title":"Hum. Gene Ther."},{"issue":"10","key":"10.1016\/j.jbiotec.2022.11.015_bib13","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1016\/j.watres.2009.03.020","article-title":"Detection of human viruses in rivers of a densly-populated area in germany using a virus adsorption elution method optimized for PCR analyses","volume":"43","author":"Hamza","year":"2009","journal-title":"Water Res."},{"issue":"11","key":"10.1016\/j.jbiotec.2022.11.015_bib14","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1089\/hum.1995.6.11-1403","article-title":"Purification of a type 5 recombinant adenovirus encoding human P53 by column chromatography","volume":"6","author":"Huyghe","year":"1995","journal-title":"Hum. Gene Ther."},{"issue":"1","key":"10.1016\/j.jbiotec.2022.11.015_bib15","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/s12985-022-01819-w","article-title":"Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation","volume":"19","author":"Kadji","year":"2022","journal-title":"Virol. J."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib16","doi-asserted-by":"crossref","first-page":"S184","DOI":"10.1002\/jgm.503","article-title":"Development and optimization of an adenovirus production process","volume":"6","author":"Kamen","year":"2004","journal-title":"J. Gene Med."},{"issue":"3","key":"10.1016\/j.jbiotec.2022.11.015_bib17","doi-asserted-by":"crossref","first-page":"157","DOI":"10.7883\/yoken1952.47.157","article-title":"A simple and efficient method for purification of infectious recombinant adenovirus","volume":"47","author":"Kanegae","year":"1994","journal-title":"Jpn. J. Med. Sci. Biol."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib18","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/bs.pmbts.2014.10.012","article-title":"Molecular determinants of the ratio of inert to infectious virus particles","volume":"129","author":"Klasse","year":"2015","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"issue":"7830","key":"10.1016\/j.jbiotec.2022.11.015_bib19","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1038\/s41586-020-2798-3","article-title":"SARS-CoV-2 vaccines in development","volume":"586","author":"Krammer","year":"2020","journal-title":"Nature"},{"issue":"8","key":"10.1016\/j.jbiotec.2022.11.015_bib20","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1038\/mt.2009.130","article-title":"New insights on adenovirus as vaccine vectors","volume":"17","author":"Lasaro","year":"2009","journal-title":"Mol. Ther."},{"issue":"1","key":"10.1016\/j.jbiotec.2022.11.015_bib21","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s12250-020-00230-5","article-title":"On the Calculation of TCID50 for Quantitation of Virus Infectivity","volume":"36","author":"Lei","year":"2021","journal-title":"Virol. Sin."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib22","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.pep.2015.04.011","article-title":"A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus","volume":"113","author":"Li","year":"2015","journal-title":"Protein Expr. Purif."},{"issue":"1\u20133","key":"10.1016\/j.jbiotec.2022.11.015_bib23","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0166-0934(94)90184-8","article-title":"Quantification of adenovirus particles","volume":"50","author":"Liebermann","year":"1994","journal-title":"J. Virol. Methods"},{"issue":"2","key":"10.1016\/j.jbiotec.2022.11.015_bib24","doi-asserted-by":"crossref","first-page":"42","DOI":"10.3390\/diseases6020042","article-title":"Viral vectors in gene therapy","volume":"6","author":"Lundstrom","year":"2018","journal-title":"Diseases"},{"key":"10.1016\/j.jbiotec.2022.11.015_bib25","first-page":"405","article-title":"Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications","volume":"Vol. 463","author":"McCue","year":"2009"},{"issue":"11","key":"10.1016\/j.jbiotec.2022.11.015_bib26","doi-asserted-by":"crossref","first-page":"3523","DOI":"10.1007\/s00705-017-3513-z","article-title":"Virus purification by CsCl density gradient using general centrifugation","volume":"162","author":"Nasukawa","year":"2017","journal-title":"Arch. Virol."},{"issue":"5","key":"10.1016\/j.jbiotec.2022.11.015_bib27","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1002\/bit.25545","article-title":"Improved virus purification processes for vaccines and gene therapy","volume":"112","author":"Nestola","year":"2015","journal-title":"Biotechnol. Bioeng."},{"issue":"6","key":"10.1016\/j.jbiotec.2022.11.015_bib28","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1002\/btpr.25","article-title":"Towards purification of adenoviral vectors based on membrane technology","volume":"24","author":"Peixoto","year":"2008","journal-title":"Biotechnol. Prog."},{"issue":"2","key":"10.1016\/j.jbiotec.2022.11.015_bib29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0168-1656(01)00237-1","article-title":"Hydrophobic interaction chromatography of proteins","volume":"87","author":"Queiroz","year":"2001","journal-title":"J. Biotechnol."},{"issue":"1","key":"10.1016\/j.jbiotec.2022.11.015_bib30","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1016\/j.xphs.2019.10.063","article-title":"Algorithm-based liquid formulation development including a DoE concept predicts long-term viral vector stability","volume":"109","author":"Reinauer","year":"2020","journal-title":"J. Pharm. Sci."},{"issue":"1","key":"10.1016\/j.jbiotec.2022.11.015_bib31","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/0021-9797(68)90272-5","article-title":"Controlled growth of monodisperse silica spheres in the micron size range","volume":"26","author":"St\u00f6ber","year":"1968","journal-title":"J. Colloid Interface Sci."},{"issue":"4","key":"10.1016\/j.jbiotec.2022.11.015_bib32","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1089\/hgtb.2015.051","article-title":"Comparative analysis of cesium chloride- and iodixanol-based purification of recombinant adeno-associated viral vectors for preclinical applications","volume":"26","author":"Strobel","year":"2015","journal-title":"Hum. Gene Ther. Methods"},{"issue":"3","key":"10.1016\/j.jbiotec.2022.11.015_bib33","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1021\/bp049561a","article-title":"Pilot-Scale adenovirus seed production through concurrent virus release and concentration by hollow fiber filtration","volume":"21","author":"Subramanian","year":"2008","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib34","first-page":"27","article-title":"Affinity chromatography accelerates viral vector purification for gene therapies","volume":"Vol. 2","author":"Terova","year":"2017","journal-title":"Biopharm. Int."},{"issue":"7","key":"10.1016\/j.jbiotec.2022.11.015_bib35","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1002\/bit.26972","article-title":"Adenovirus 5 recovery using nanofiber ion\u2010exchange adsorbents","volume":"116","author":"Turnbull","year":"2019","journal-title":"Biotechnol. Bioeng."},{"issue":"4","key":"10.1016\/j.jbiotec.2022.11.015_bib36","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/j.bbrc.2005.03.227","article-title":"Purification of infectious adenovirus in two hours by ultracentrifugation and tangential flow filtration","volume":"331","author":"Ugai","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jbiotec.2022.11.015_bib37","first-page":"1","article-title":"Introduction to viral vectors","volume":"Vol. 737","author":"Warnock","year":"2011"},{"issue":"13","key":"10.1016\/j.jbiotec.2022.11.015_bib38","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1016\/j.chroma.2008.11.010","article-title":"Rapid high-performance liquid chromatographic analysis of adenovirus type 5 particles with a prototype anion-exchange analytical monolith column","volume":"1216","author":"Whitfield","year":"2009","journal-title":"J. Chromatogr. A"},{"issue":"6","key":"10.1016\/j.jbiotec.2022.11.015_bib39","doi-asserted-by":"crossref","first-page":"421","DOI":"10.2174\/1566523213666131125095046","article-title":"Adenovirus vectors for gene therapy, vaccination and cancer gene therapy","volume":"13","author":"Wold","year":"2014","journal-title":"Curr. Gene Ther."},{"issue":"36","key":"10.1016\/j.jbiotec.2022.11.015_bib40","doi-asserted-by":"crossref","first-page":"5491","DOI":"10.1016\/j.vaccine.2018.02.090","article-title":"Affinity chromatography for vaccines manufacturing: finally ready for prime time?","volume":"37","author":"Zhao","year":"2019","journal-title":"Vaccine"}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165622002760?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165622002760?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T13:00:07Z","timestamp":1759150807000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165622002760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":40,"alternative-id":["S0168165622002760"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2022.11.015","relation":{},"ISSN":["0168-1656"],"issn-type":[{"value":"0168-1656","type":"print"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An efficient capture strategy for the purification of human adenovirus type 5 from cell lysates","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2022.11.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}