{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T15:31:16Z","timestamp":1759159876012,"version":"3.37.3"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["EFRE-0400138"],"award-info":[{"award-number":["EFRE-0400138"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010812","name":"Fachagentur Nachwachsende Rohstoffe","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010812","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005721","name":"Universit\u00e4t Bielefeld","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005721","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020631","name":"Bundesministerium f\u00fcr Ern\u00e4hrung und Landwirtschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020631","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005908","name":"Bundesministerium f\u00fcr Ern\u00e4hrung und Landwirtschaft","doi-asserted-by":"publisher","award":["22001716"],"award-info":[{"award-number":["22001716"]}],"id":[{"id":"10.13039\/501100005908","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2023,4]]},"DOI":"10.1016\/j.jbiotec.2023.02.007","type":"journal-article","created":{"date-parts":[[2023,3,11]],"date-time":"2023-03-11T01:42:04Z","timestamp":1678498924000},"page":"81-88","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Database-driven in silico-identification and characterization of novel aldoxime dehydratases"],"prefix":"10.1016","volume":"367","author":[{"given":"Michael","family":"Hinzmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilmi","family":"Yavuzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessa","family":"Hinzmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Gr\u00f6ger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiotec.2023.02.007_bib1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0168-1656(01)00419-9","article-title":"Overview of screening for new microbial catalysts and their uses in organic synthesis\u2014selection and optimization of biocatalysts","volume":"94","author":"Asano","year":"2002","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/j.1574-6968.1998.tb12818.x","article-title":"Z phenylacetaldoxime degradation by a novel aldoxime dehydratase from Bacillus sp. strain OxB-1","volume":"158","author":"Asano","year":"1998","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib3","doi-asserted-by":"crossref","first-page":"12361","DOI":"10.1002\/anie.201702952","article-title":"Cyanide-free and broadly applicable enantioselective synthetic platform for chiral nitriles through a biocatalytic approach","volume":"56","author":"Betke","year":"2017","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib4","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1002\/cbic.201700571","article-title":"Biocatalytic synthesis of nitriles through dehydration of aldoximes: the substrate scope of aldoxime dehydratases","volume":"19","author":"Betke","year":"2018","journal-title":"ChemBioChem"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.enzmictec.2021.109883","article-title":"Cyanide-free synthesis of aromatic nitriles from aldoximes: discovery and application of a novel heme-containing aldoxime dehydratase","volume":"150","author":"Chen","year":"2021","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib6","doi-asserted-by":"crossref","first-page":"362","DOI":"10.3390\/catal10040362","article-title":"Aldoxime dehydratase mutants as improved biocatalysts for a sustainable synthesis of biorenewables-based 2-furonitrile","volume":"10","author":"Choi","year":"2020","journal-title":"Catalysts"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib7","first-page":"26","article-title":"Nitrile synthesis with aldoxime dehydratases: a biocatalytic platform with applications in asymmetric synthesis, bulk chemicals, and biorefineries","author":"Dom\u00ednguez de Mar\u00eda","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib8","doi-asserted-by":"crossref","first-page":"16729","DOI":"10.1021\/bi0511585","article-title":"Spectroscopic and biochemical characterization of heme binding to yeast Dap1p and mouse PGRMC1p","volume":"44","author":"Ghosh","year":"2005","journal-title":"Biochemistry"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib9","doi-asserted-by":"crossref","first-page":"4867","DOI":"10.1021\/acs.joc.9b00184","article-title":"Enzymatic synthesis of aliphatic nitriles at a substrate loading of up to 1.4 kg\/L: a biocatalytic record achieved with a heme protein","volume":"84","author":"Hinzmann","year":"2019","journal-title":"J. Org. Chem."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib10","doi-asserted-by":"crossref","first-page":"17088","DOI":"10.1021\/acssuschemeng.0c04981","article-title":"Chemoenzymatic cascades toward aliphatic nitriles starting from biorenewable feedstocks","volume":"8","author":"Hinzmann","year":"2020","journal-title":"ACS Sustain. Chem. Eng."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib11","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.3390\/catal10091073","article-title":"Immobilization of aldoxime dehydratases and their use as biocatalysts in aqueous reaction media","volume":"10","author":"Hinzmann","year":"2020","journal-title":"Catalysts"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib12","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1039\/D1CY01694F","article-title":"Chemoenzymatic one-pot reaction from carboxylic acid to nitrile via oxime","volume":"12","author":"Horvat","year":"2022","journal-title":"Catal. Sci. Technol."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib13","doi-asserted-by":"crossref","first-page":"2254","DOI":"10.1271\/bbb.69.2254","article-title":"Purification and characterization of aldoxime dehydratase of the head blight fungus, fusarium graminearum","volume":"69","author":"Kato","year":"2005","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib14","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s002030050618","article-title":"Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes","volume":"170","author":"Kato","year":"1998","journal-title":"Arch. Microbiol."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib15","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/S1389-1723(04)70200-5","article-title":"Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771","volume":"97","author":"Kato","year":"2004","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib16","doi-asserted-by":"crossref","first-page":"5563","DOI":"10.1007\/s00253-022-12071-1","article-title":"Characterization of proteins from the 3N5M family reveals an operationally stable amine transaminase","volume":"106","author":"Kollipara","year":"2022","journal-title":"Appl. Microbiol Biotechnol."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.enzmictec.2022.110187","article-title":"Scanning aldoxime dehydratase sequence space and characterization of a new aldoxime dehydratase from Fusarium vanettenii","volume":"164","author":"K\u0159\u00edstkov\u00e1","year":"2023","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib18","first-page":"2101","article-title":"3DM: systematic analysis of heterogeneous superfamily data to discover protein functionalities","volume":"78","author":"Kuipers","year":"2010","journal-title":"Proteins"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinorgbio.2022.111770","article-title":"Crystal structural analysis of aldoxime dehydratase from Bacillus sp. OxB-1: importance of surface residues in optimization for crystallization","volume":"230","author":"Matsui","year":"2022","journal-title":"J. Inorg. Biochem."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib20","doi-asserted-by":"crossref","first-page":"2810","DOI":"10.1073\/pnas.1200338110","article-title":"Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis","volume":"110","author":"Nomura","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib21","doi-asserted-by":"crossref","first-page":"29600","DOI":"10.1074\/jbc.M211832200","article-title":"Novel aldoxime dehydratase involved in carbon-nitrogen triple bond synthesis of pseudomonas chlororaphis B23","volume":"278","author":"Oinuma","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib22","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.ijbiomac.2018.04.103","article-title":"Overproduction and characterization of the first enzyme of a new aldoxime dehydratase family in bradyrhizobium sp","volume":"115","author":"R\u00e4disch","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib23","doi-asserted-by":"crossref","first-page":"549","DOI":"10.3390\/microorganisms10030549","article-title":"Metabolism of aidoximes and nitriles in plant-associated bacteria and its potential in plant-bacteria interactions","volume":"10","author":"R\u00e4disch","year":"2022","journal-title":"Microorganisms"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib24","doi-asserted-by":"crossref","first-page":"32089","DOI":"10.1074\/jbc.M109.018762","article-title":"X-ray crystal structure of michaelis complex of aldoxime dehydratase","volume":"284","author":"Sawai","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib25","first-page":"1269","article-title":"Analyse spectrale: sur le spectre d\u2032absorption du sang dans la partie violette et ultra-violette","author":"Soret","year":"1883","journal-title":"Comptes Rendus l\u2032Acad. Des. Sci."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib26","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/adsc.202201053","article-title":"Organic acid to nitrile: a chemoenzymatic three\u2010step route","volume":"365","author":"Winkler","year":"2023","journal-title":"Adv. Synth. Catal."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib27","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1128\/genomeA.00025-15","article-title":"Complete genome sequence of an aldoxime degrader, Bacillus sp. OxB-1","volume":"3","author":"Yamaguchi","year":"2015","journal-title":"Genome Announc."},{"year":"2021","series-title":"Combination of Computational and Experimental Approaches to Rationalize and Modify Aldoxime Dehydratase Catalyzed Reactions and Their Application","author":"Yavuzer","key":"10.1016\/j.jbiotec.2023.02.007_bib28"},{"key":"10.1016\/j.jbiotec.2023.02.007_bib29","doi-asserted-by":"crossref","first-page":"19162","DOI":"10.1002\/anie.202017234","article-title":"Rationalizing the unprecedented stereochemistry of an Enzymatic Nitrile Synthesis through a combined computational and experimental approach","volume":"60","author":"Yavuzer","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.jbiotec.2023.02.007_bib30","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkr1288","article-title":"SLiCE: a novel bacterial cell extract-based DNA cloning method","volume":"40","author":"Zhang","year":"2012","journal-title":"Nucleic Acids Res."}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165623000408?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165623000408?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T11:38:15Z","timestamp":1736336295000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165623000408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4]]},"references-count":30,"alternative-id":["S0168165623000408"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2023.02.007","relation":{},"ISSN":["0168-1656"],"issn-type":[{"type":"print","value":"0168-1656"}],"subject":[],"published":{"date-parts":[[2023,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Database-driven in silico-identification and characterization of novel aldoxime dehydratases","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2023.02.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}