{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T03:56:55Z","timestamp":1740110215413,"version":"3.37.3"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T00:00:00Z","timestamp":1727827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000161","name":"Networks of Centres of Excellence of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000161","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004489","name":"Mitacs","doi-asserted-by":"publisher","award":["IT23247"],"award-info":[{"award-number":["IT23247"]}],"id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.jbiotec.2024.09.018","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T16:28:32Z","timestamp":1727368112000},"page":"216-227","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Expediting adenovirus titer assays via an algorithmic live-cell imaging technique"],"prefix":"10.1016","volume":"395","author":[{"given":"Claire","family":"Velikonja","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Landon","family":"Steenbakkers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua","family":"How","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mackenzie","family":"Enns","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon","family":"Corbett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"McCready","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jake","family":"Nease","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prashant","family":"Mhaskar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Latulippe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiotec.2024.09.018_bib1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.625136","article-title":"An optimized high-throughput immuno-plaque assay for SARS-CoV-2","volume":"vol. 12","author":"Amarilla","year":"2021","journal-title":"Front. Microbiol."},{"issue":"7","key":"10.1016\/j.jbiotec.2024.09.018_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/v13071193","article-title":"A fluorescent real-time plaque assay enables single-cell analysis of virus-induced cytopathic effect by live-cell imaging","volume":"vol. 13","author":"Arias-Arias","year":"2021","journal-title":"Viruses"},{"issue":"5","key":"10.1016\/j.jbiotec.2024.09.018_bib3","doi-asserted-by":"crossref","first-page":"870","DOI":"10.2144\/00285bm08","article-title":"Accelerated titering of adenoviruses","volume":"vol. 28","author":"Bewig","year":"2000","journal-title":"BioTechniques"},{"key":"10.1016\/j.jbiotec.2024.09.018_bib4","unstructured":"I.Essen Bioscience, IncuCyte Live-Cell Analysis Systems User Manual.\u201d Sartorius, Inc, 2022. [Online]. Available: \u3008https:\/\/www.sartorius.com\/download\/1087348\/incucyte-live-cell-analysis-systems-user-manual-en-l-8000-04-1--data.pdf\u3009."},{"issue":"1","key":"10.1016\/j.jbiotec.2024.09.018_bib5","article-title":"Viral vector platforms within the gene therapy landscape","volume":"vol. 6","author":"Bulcha","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib6","unstructured":"Cell Bio Labs, Inc, QuickTiterTM Adenovirus Titer Immunoassay Kit.\u201d Cell Bio Labs, Inc, 2004. [Online]. Available: \u3008https:\/\/www.cellbiolabs.com\/sites\/default\/files\/CBD31A2F-3048-812A-2E9D276F8538B02B.pdf\u3009."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib7","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.omtm.2023.05.019","article-title":"Mechanistic modeling explains the production dynamics of recombinant adeno-associated virus with the baculovirus expression vector system","volume":"vol. 30","author":"Destro","year":"2023","journal-title":"Mol. Ther. - Methods Clin. Dev."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1023\/A:1021106116887","article-title":"Rapid titration of adenoviral infectivity by flow cytometry in batch culture of infected HEK293 cells","volume":"vol. 38","author":"Gueret","year":"2002","journal-title":"Cytotechnology"},{"issue":"6","key":"10.1016\/j.jbiotec.2024.09.018_bib9","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1021\/acssensors.9b00489","article-title":"Detection and quantification of label-free infectious adenovirus using a switch-on cell-based fluorescent biosensor","volume":"vol. 4","author":"Guerreiro","year":"2019","journal-title":"ACS Sens"},{"issue":"6","key":"10.1016\/j.jbiotec.2024.09.018_bib10","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1006\/mthe.2000.0217","article-title":"Working toward an adenoviral vector testing standard","volume":"vol. 2","author":"Hutchins","year":"2000","journal-title":"Mol. Ther."},{"issue":"1","key":"10.1016\/j.jbiotec.2024.09.018_bib11","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/bit.28553","article-title":"Accelerated and intensified manufacturing of an adenovirus-vectored vaccine to enable rapid outbreak response","volume":"vol. 121","author":"Joe","year":"2024","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2022.108417","article-title":"Comparison of the performance of anion exchange membrane materials for adenovirus purification using laterally-fed membrane chromatography","volume":"vol. 182","author":"Kawka","year":"2022","journal-title":"Biochem. Eng. J."},{"issue":"7","key":"10.1016\/j.jbiotec.2024.09.018_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0254739","article-title":"Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach","volume":"vol. 16","author":"Labisch","year":"2021","journal-title":"PLOS ONE"},{"issue":"3","key":"10.1016\/j.jbiotec.2024.09.018_bib14","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1089\/adt.2018.881","article-title":"High-throughput zika viral titer assay for rapid screening of antiviral drugs","volume":"vol. 17","author":"Lee","year":"2019","journal-title":"ASSAY Drug Dev. Technol."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib15","doi-asserted-by":"crossref","unstructured":"M. Leslie, Tumor-killing viruses score rare success in late-stage trial, Science. Accessed: Mar. 25, 2024. [Online]. Available: \u3008https:\/\/www.science.org\/content\/article\/tumor-killing-viruses-score-rare-success-late-stage-trial\u3009.","DOI":"10.1126\/science.adn3528"},{"issue":"8","key":"10.1016\/j.jbiotec.2024.09.018_bib16","doi-asserted-by":"crossref","DOI":"10.1038\/s41551-023-01057-7","article-title":"Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning","volume":"vol. 7","author":"Liu","year":"2023","journal-title":"Nat. Biomed. Eng."},{"issue":"2","key":"10.1016\/j.jbiotec.2024.09.018_bib17","doi-asserted-by":"crossref","DOI":"10.1136\/jitc-2020-001486","article-title":"Clinical landscape of oncolytic virus research in 2020","volume":"vol. 8","author":"Macedo","year":"2020","journal-title":"J. Immunother. Cancer"},{"issue":"2","key":"10.1016\/j.jbiotec.2024.09.018_bib18","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.jviromet.2013.04.008","article-title":"Virus quantitation by transmission electron microscopy, TCID50, and the role of timing virus harvesting: a case study of three animal viruses","volume":"vol. 191","author":"Malenovska","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jbiotec.2024.09.018_bib19","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.omtm.2019.07.007","article-title":"Integration of fluorescence detection and image-based automated counting increases speed, sensitivity, and robustness of plaque assays","volume":"vol. 14","author":"Masci","year":"2019","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib20","unstructured":"D. Murphy, Guidance for Human Somatic Cell Therapy and Gene Therapy.\u201d U.S. Food and Drug Administration Center for Biologics Evaluation and Research, Mar. 1998. [Online]. Available: \u3008https:\/\/www.fda.gov\/media\/72402\/download\u3009."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib21","unstructured":"Regenerative Medicine: The Pipeline Momentum Builds.\u201d Alliance for Regenerative Medicine, Sep. 2022. [Online]. Available: \u3008https:\/\/alliancerm.org\/wp-content\/uploads\/2022\/10\/ARM-H1-2022-R13.pdf\u3009."},{"issue":"5","key":"10.1016\/j.jbiotec.2024.09.018_bib22","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1213\/ANE.0000000000002864","article-title":"Correlation coefficients: appropriate use and interpretation","volume":"vol. 126","author":"Schober","year":"2018","journal-title":"Anesth. Analg."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib23","unstructured":"Sector Snapshot.\u201d Alliance for Regenerative Medicine, Apr. 2023. [Online]. Available: \u3008https:\/\/alliancerm.org\/wp-content\/uploads\/2023\/04\/WEB_ARM_Sector-Snapshot_Report_R02_Web.pdf\u3009."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib24","unstructured":"J. Sterling, Cell and Gene Therapy Manufacturing Costs Limiting Access, GEN - Genetic Engineering and Biotechnology News. Accessed: Mar. 28, 2024. [Online]. Available: \u3008https:\/\/www.genengnews.com\/insights\/cell-and-gene-therapy-manufacturing-costs-limiting-access\/\u3009."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib25","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jviromet.2015.03.009","article-title":"A novel method for the measurement of hepatitis C virus infectious titres using the IncuCyte ZOOM and its application to antiviral screening","volume":"vol. 218","author":"Stewart","year":"2015","journal-title":"J. Virol. Methods"},{"issue":"5","key":"10.1016\/j.jbiotec.2024.09.018_bib26","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1007\/s00253-015-7267-9","article-title":"Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production","volume":"vol. 100","author":"Tapia","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"3","key":"10.1016\/j.jbiotec.2024.09.018_bib27","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.vascn.2004.08.014","article-title":"HEK293 cell line: a vehicle for the expression of recombinant proteins","volume":"vol. 51","author":"Thomas","year":"2005","journal-title":"J. Pharmacol. Toxicol. Methods"},{"issue":"R1","key":"10.1016\/j.jbiotec.2024.09.018_bib28","doi-asserted-by":"crossref","first-page":"R42","DOI":"10.1093\/hmg\/ddv451","article-title":"Progress and challenges in viral vector manufacturing","volume":"vol. 25","author":"Van Der Loo","year":"2016","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.jbiotec.2024.09.018_bib29","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jchromb.2014.07.025","article-title":"Separation of porcine parvovirus from bovine serum albumin using PEG\u2013salt aqueous two-phase system","volume":"vol. 967","author":"Vijayaragavan","year":"2014","journal-title":"J. Chromatogr. B"},{"issue":"36","key":"10.1016\/j.jbiotec.2024.09.018_bib30","doi-asserted-by":"crossref","first-page":"eaba9319","DOI":"10.1126\/sciadv.aba9319","article-title":"Live-cell imaging and analysis reveal cell phenotypic transition dynamics inherently missing in snapshot data","volume":"vol. 6","author":"Wang","year":"2020","journal-title":"Sci. Adv."},{"issue":"3","key":"10.1016\/j.jbiotec.2024.09.018_bib31","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1006\/meth.2000.1010","article-title":"Evaluation of adenoviral vectors by flow cytometry","volume":"vol. 21","author":"Weaver","year":"2000","journal-title":"Methods"},{"key":"10.1016\/j.jbiotec.2024.09.018_bib32","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.jviromet.2018.10.022","article-title":"Development and application of a higher throughput RSV plaque assay by immunofluorescent imaging","volume":"vol. 263","author":"Wen","year":"2019","journal-title":"J. Virol. Methods"},{"issue":"8","key":"10.1016\/j.jbiotec.2024.09.018_bib33","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines9081032","article-title":"Efficient selection of recombinant fluorescent vaccinia virus strains and rapid virus titer determination by using a multi-well plate imaging system","volume":"vol. 9","author":"Ye","year":"2021","journal-title":"Biomedicines"}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165624002591?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165624002591?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T02:25:54Z","timestamp":1730341554000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165624002591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":33,"alternative-id":["S0168165624002591"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2024.09.018","relation":{},"ISSN":["0168-1656"],"issn-type":[{"type":"print","value":"0168-1656"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expediting adenovirus titer assays via an algorithmic live-cell imaging technique","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2024.09.018","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}