{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:57:14Z","timestamp":1779209834310,"version":"3.51.4"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"EU Framework Programme for Research and Innovation Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["955740"],"award-info":[{"award-number":["955740"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"ANR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007601","name":"Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.jbiotec.2025.03.011","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T21:19:09Z","timestamp":1742505549000},"page":"1-8","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Comparative characterisation of autotrophic and heterotrophic isopropanol formation by Cupriavidus necator in shake flasks"],"prefix":"10.1016","volume":"403","author":[{"given":"I.","family":"Weickardt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Lombard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Blank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.E.","family":"Guillouet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.jbiotec.2025.03.011_bib1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s11306-017-1302-z","article-title":"13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16","volume":"14","author":"Alagesan","year":"2018","journal-title":"Metabolomics"},{"issue":"2","key":"10.1016\/j.jbiotec.2025.03.011_bib2","doi-asserted-by":"crossref","DOI":"10.1128\/aem.02007-22","article-title":"Minimizing the lag phase of Cupriavidus necator growth under autotrophic, heterotrophic, and mixotrophic conditions","volume":"89","author":"Amer","year":"2023","journal-title":"Appl. Environ. Microbiol."},{"issue":"1","key":"10.1016\/j.jbiotec.2025.03.011_bib3","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/1475-2859-12-107","article-title":"Development of a broad-host synthetic biology toolbox for Ralstonia eutropha and its application to engineering hydrocarbon biofuel production","volume":"12","author":"Bi","year":"2013","journal-title":"Micro Cell Fact."},{"issue":"1","key":"10.1016\/j.jbiotec.2025.03.011_bib4","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/s13568-021-01307-4","article-title":"Investigation of the robustness of Cupriavidus necator engineered strains during fed-batch cultures","volume":"11","author":"Boy","year":"2021","journal-title":"AMB Expr."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jbiotec.2023.02.011","article-title":"Comparison of plasmid stabilization systems during heterologous isopropanol production in fed-batch bioreactor","volume":"366","author":"Boy","year":"2023","journal-title":"J. Biotechnol."},{"issue":"9","key":"10.1016\/j.jbiotec.2025.03.011_bib6","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1128\/AEM.02429-10","article-title":"Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains","volume":"77","author":"Budde","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"issue":"1-2","key":"10.1016\/j.jbiotec.2025.03.011_bib7","first-page":"38","article-title":"Genomic view of energy metabolism in Ralstonia eutropha H16. Journal of molecular microbiology and biotechnology","volume":"16","author":"Cramm","year":"2009"},{"key":"10.1016\/j.jbiotec.2025.03.011_bib8","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ymben.2016.05.002","article-title":"Metabolic engineering of Cupriavidus necator for heterotrophic and autotrophic alka(e)ne production","volume":"37","author":"Cr\u00e9pin","year":"2016","journal-title":"Metab. Eng."},{"issue":"3","key":"10.1016\/j.jbiotec.2025.03.011_bib9","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1128\/jb.145.3.1144-1149.1981","article-title":"Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus","volume":"145","author":"Friedrich","year":"1981","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib10","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.nbt.2019.11.005","article-title":"Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor","volume":"56","author":"Garrigues","year":"2020","journal-title":"N. Biotechnol."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib11","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.biortech.2013.08.120","article-title":"Impact of sustaining a controlled residual growth on polyhydroxybutyrate yield and production kinetics in Cupriavidus necator","volume":"148","author":"Grousseau","year":"2013","journal-title":"Bioresour. Technol."},{"issue":"9","key":"10.1016\/j.jbiotec.2025.03.011_bib12","doi-asserted-by":"crossref","first-page":"4277","DOI":"10.1007\/s00253-014-5591-0","article-title":"Isopropanol production with engineered Cupriavidus necator as bioproduction platform","volume":"98","author":"Grousseau","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib13","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ymben.2022.02.003","article-title":"Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production","volume":"72","author":"Hanko","year":"2022","journal-title":"Metab. Eng."},{"issue":"1-2","key":"10.1016\/j.jbiotec.2025.03.011_bib14","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1007\/s002530100775","article-title":"Microbial production of poly-D-3-hydroxybutyrate from CO2","volume":"57","author":"Ishizaki","year":"2001","journal-title":"Appl. Microbiol Biotechnol."},{"issue":"7","key":"10.1016\/j.jbiotec.2025.03.011_bib15","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1002\/anie.201711302","article-title":"CO2 to terpenes: autotrophic and electroautotrophic \u03b1-humulene production with Cupriavidus necator","volume":"57","author":"Krieg","year":"2018","journal-title":"Angew. Chem. Int. Ed."},{"issue":"6","key":"10.1016\/j.jbiotec.2025.03.011_bib16","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1007\/s10295-019-02156-8","article-title":"Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory","volume":"46","author":"Li","year":"2019","journal-title":"J. Ind. Microbiol. Biotechnol."},{"issue":"3","key":"10.1016\/j.jbiotec.2025.03.011_bib17","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/s41587-021-01195-w","article-title":"Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale","volume":"40","author":"Liew","year":"2022","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"10.1016\/j.jbiotec.2025.03.011_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0163-7258(96)00198-2","article-title":"Structure and mechanism of carbonic anhydrase","volume":"74","author":"Lindskog","year":"1997","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib19","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ymben.2017.05.007","article-title":"Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production","volume":"42","author":"Marc","year":"2017","journal-title":"Metab. Eng."},{"issue":"2","key":"10.1016\/j.jbiotec.2025.03.011_bib20","first-page":"439","article-title":"Growth characteristics and cell composition of Alcaligenes eutrophus in chemostat culture","volume":"42","author":"Morinaga","year":"1978","journal-title":"Agric. Biol. Chem."},{"issue":"14","key":"10.1016\/j.jbiotec.2025.03.011_bib21","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1128\/AEM.00973-13","article-title":"Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones","volume":"79","author":"M\u00fcller","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib22","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ymben.2020.09.002","article-title":"Valorization of CO2 through lithoautotrophic production of sustainable chemicals in Cupriavidus necator","volume":"62","author":"Nangle","year":"2020","journal-title":"Metab. Eng."},{"issue":"2","key":"10.1016\/j.jbiotec.2025.03.011_bib23","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/biot.200900220","article-title":"Advanced biofuel production in microbes","volume":"5","author":"Peralta-Yahya","year":"2010","journal-title":"Biotechnol. J."},{"issue":"5","key":"10.1016\/j.jbiotec.2025.03.011_bib24","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1007\/s00253-014-6266-6","article-title":"Exploiting mixtures of H2, CO2, and O2 for improved production of methacrylate precursor 2-hydroxyisobutyric acid by engineered Cupriavidus necator strains","volume":"99","author":"Przybylski","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"4","key":"10.1016\/j.jbiotec.2025.03.011_bib25","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1128\/aem.32.4.585-591.1976","article-title":"Quantitative requirements for exponential growth of Alcaligenes eutrophus","volume":"32","author":"Repaske","year":"1976","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib26","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.ymben.2022.10.014","article-title":"Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid","volume":"74","author":"Salinas","year":"2022","journal-title":"Metab. Eng."},{"issue":"4787","key":"10.1016\/j.jbiotec.2025.03.011_bib27","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1038\/191463a0","article-title":"Formation and utilization of poly-beta-hydroxybutyric acid by Knallgas bacteria (Hydrogenomonas)","volume":"191","author":"Schlegel","year":"1961","journal-title":"Nature"},{"key":"10.1016\/j.jbiotec.2025.03.011_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.130556","article-title":"Biological carbon capture from biogas streams: Insights into Cupriavidus necator autotrophic growth and transcriptional profile","volume":"399","author":"Serna-Garc\u00eda","year":"2024","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.jbiotec.2025.03.011_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2019.107369","article-title":"Carbon dioxide fixation by a hydrogen-oxidizing bacterium: Biomass yield, reversal respiratory quotient, stoichiometric equations and bioenergetics","volume":"152","author":"Yu","year":"2019","journal-title":"Biochem. Eng. J."}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165625000707?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165625000707?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T20:30:08Z","timestamp":1746822608000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165625000707"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":29,"alternative-id":["S0168165625000707"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2025.03.011","relation":{},"ISSN":["0168-1656"],"issn-type":[{"value":"0168-1656","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparative characterisation of autotrophic and heterotrophic isopropanol formation by Cupriavidus necator in shake flasks","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2025.03.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}