{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T14:18:13Z","timestamp":1754144293802,"version":"3.41.2"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["ECS00000036","DM 352"],"award-info":[{"award-number":["ECS00000036","DM 352"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006692","name":"Universita degli Studi di Torino","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.jbiotec.2025.04.024","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T12:04:29Z","timestamp":1746533069000},"page":"8-16","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Biocatalytic production of a monoamine oxidase B\/catechol-O-methyltransferase inhibitor from piperine by engineered P450 BM3."],"prefix":"10.1016","volume":"405","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7656-9518","authenticated-orcid":false,"given":"Mariusz","family":"Brzoski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8005-0398","authenticated-orcid":false,"given":"Samuele","family":"Irudal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Gazzano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4577-3635","authenticated-orcid":false,"given":"Roberto","family":"Buscaino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9387-2849","authenticated-orcid":false,"given":"Guido","family":"Viscardi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-2635","authenticated-orcid":false,"given":"Giovanna","family":"Di Nardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianfranco","family":"Gilardi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"11","key":"10.1016\/j.jbiotec.2025.04.024_bib1","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1021\/cn300077c","article-title":"Role of ABC transporters in the pathogenesis of Alzheimer\u2019s disease","volume":"3","author":"Abuznait","year":"2012","journal-title":"ACS Chem. Neurosci."},{"issue":"September","key":"10.1016\/j.jbiotec.2025.04.024_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fitote.2014.05.005","article-title":"Role of herbal bioactives as a potential bioavailability enhancer for active pharmaceutical ingredients","volume":"97","author":"Ajazuddin","year":"2014","journal-title":"Fitoterapia"},{"issue":"23","key":"10.1016\/j.jbiotec.2025.04.024_bib3","doi-asserted-by":"crossref","first-page":"7183","DOI":"10.1016\/j.bmcl.2012.09.056","article-title":"Inhibition of monoamine oxidase by derivatives of piperine, an alkaloid from the pepper plant piper nigrum, for possible use in Parkinson\u2019s disease","volume":"22","author":"Al-Baghdadi","year":"2012","journal-title":"Bioorg. Med. Chem. Lett."},{"issue":"4","key":"10.1016\/j.jbiotec.2025.04.024_bib4","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1124\/dmd.113.056176","article-title":"\u2018Inhibition of the Multidrug Resistance P-Glycoprotein: time for a Change of Strategy","volume":"42","author":"Callaghan","year":"2014","journal-title":"Drug Metab. Dispos."},{"issue":"1","key":"10.1016\/j.jbiotec.2025.04.024_bib5","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1517\/13543776.2014.982535","article-title":"\u2018Novel monoamine oxidase inhibitors: a patent review (2012 \u2013 2014","volume":"25","author":"Carradori","year":"2015","journal-title":"Expert Opin. Ther. Pat."},{"issue":"1","key":"10.1016\/j.jbiotec.2025.04.024_bib6","doi-asserted-by":"crossref","first-page":"184","DOI":"10.3390\/ddc3010011","article-title":"Plant-derived natural products: a source for drug discovery and development","volume":"3","author":"Chaachouay","year":"2024","journal-title":"Drugs Drug Candidates"},{"issue":"6","key":"10.1016\/j.jbiotec.2025.04.024_bib7","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1021\/acs.accounts.0c00810","article-title":"Chemoenzymatic TOtal Synthesis of Natural Products","volume":"54","author":"Chakrabarty","year":"2021","journal-title":"Acc. Chem. Res."},{"issue":"December","key":"10.1016\/j.jbiotec.2025.04.024_bib8","article-title":"Boosting caffeic acid performance as antioxidant and monoamine oxidase B\/Catechol-O-methyltransferase inhibitor","volume":"243","author":"Chavarria","year":"2022","journal-title":"Eur. J. Med. Chem."},{"issue":"1","key":"10.1016\/j.jbiotec.2025.04.024_bib9","doi-asserted-by":"crossref","first-page":"75","DOI":"10.2174\/1874842201603010075","article-title":"Piperine and its various physicochemical and biological aspects: a review","volume":"3","author":"Chopra","year":"2016","journal-title":"Open Chem. J."},{"issue":"52","key":"10.1016\/j.jbiotec.2025.04.024_bib10","doi-asserted-by":"crossref","first-page":"33964","DOI":"10.1039\/D2RA06029A","article-title":"Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives","volume":"12","author":"Correddu","year":"2022","journal-title":"RSC Adv."},{"key":"10.1016\/j.jbiotec.2025.04.024_bib11","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.abb.2015.12.005","article-title":"Subtle structural changes in the Asp251Gly\/Gln307His P450 BM3 mutant responsible for new activity toward diclofenac, tolbutamide and ibuprofen","volume":"602","author":"Di Nardo","year":"2016","journal-title":"Arch. Biochem. Biophys."},{"issue":"8","key":"10.1016\/j.jbiotec.2025.04.024_bib12","doi-asserted-by":"crossref","first-page":"5560","DOI":"10.1021\/acscatal.4c00086","article-title":"Choose your own adventure: a comprehensive database of reactions catalyzed by cytochrome P450 BM3 variants","volume":"14","author":"Fansher","year":"2024","journal-title":"ACS Catal."},{"issue":"11","key":"10.1016\/j.jbiotec.2025.04.024_bib13","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1002\/rcm.7864","article-title":"Characterization of piperine metabolites in rats by ultra-high-performance liquid chromatography with electrospray ionization quadruple time-of-flight tandem mass spectrometry","volume":"31","author":"Gao","year":"2017","journal-title":"Rapid Commun. Mass Spectrom."},{"issue":"December","key":"10.1016\/j.jbiotec.2025.04.024_bib14","article-title":"Recent advances in oxidase biocatalysts: enzyme discovery, cascade reactions and scale up","volume":"38","author":"Heath","year":"2022","journal-title":"Curr. Opin. Green. Sustain. Chem."},{"issue":"5","key":"10.1016\/j.jbiotec.2025.04.024_bib15","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1002\/ppp3.10138","article-title":"Molecules from nature: reconciling biodiversity conservation and global healthcare imperatives for sustainable use of medicinal plants and fungi","volume":"2","author":"Howes","year":"2020","journal-title":"PLANTS, PEOPLE, PLANET"},{"issue":"3","key":"10.1016\/j.jbiotec.2025.04.024_bib16","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.bbagen.2006.07.003","article-title":"Complex reactions catalyzed by cytochrome P450 enzymes","volume":"1770","author":"Isin","year":"2007","journal-title":"Biochim. Et. Biophys. Acta (BBA) - Gen. Subj."},{"issue":"4","key":"10.1016\/j.jbiotec.2025.04.024_bib17","first-page":"287","article-title":"Role of ABC-cassette transporters (Mdr1, Mrp1, Bcrp) in thE Development of Primary and Acquired Multiple Drug Resistance in Patients with Early and Metastatic Breast Cancer","volume":"35","author":"Kovalev","year":"2013","journal-title":"Exp. Oncol."},{"issue":"3","key":"10.1016\/j.jbiotec.2025.04.024_bib18","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.3390\/molecules28031114","article-title":"Future antimicrobials: natural and functionalized phenolics","volume":"28","author":"Lobiuc","year":"2023","journal-title":"Molecules"},{"issue":"4","key":"10.1016\/j.jbiotec.2025.04.024_bib19","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1111\/lam.13646","article-title":"Piperine, an active ingredient of white pepper, suppresses the growth of multidrug-resistant toxigenic vibrio cholerae and other pathogenic bacteria","volume":"74","author":"Manjunath","year":"2022","journal-title":"Lett. Appl. Microbiol."},{"issue":"6","key":"10.1016\/j.jbiotec.2025.04.024_bib20","doi-asserted-by":"crossref","first-page":"1880","DOI":"10.1016\/S0021-9258(19)42868-8","article-title":"\u03c9\u20132) hydroxylation of fatty acids by a soluble system from bacillus megaterium","volume":"249","author":"Miura","year":"1974","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbiotec.2025.04.024_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.molliq.2024.126379","article-title":"Piperine analogues as dual inhibitors for antibacterial and antiarthritic properties through impact of ligands optimization, docking and water solvation","volume":"415","author":"Muhammad","year":"2024","journal-title":"J. Mol. Liq."},{"issue":"4","key":"10.1016\/j.jbiotec.2025.04.024_bib22","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.bmc.2018.12.043","article-title":"Design, synthesis and identification of novel coumaperine derivatives for inhibition of human 5-LOX: antioxidant, pseudoperoxidase and docking studies","volume":"27","author":"Muthuraman","year":"2019","journal-title":"Bioorg. Med. Chem."},{"issue":"9","key":"10.1016\/j.jbiotec.2025.04.024_bib23","doi-asserted-by":"crossref","first-page":"2490","DOI":"10.1039\/c0cc03165h","article-title":"Cytochromes P450 as useful biocatalysts: addressing the limitations","volume":"47","author":"O\u2019Reilly","year":"2011","journal-title":"Chem. Commun."},{"key":"10.1016\/j.jbiotec.2025.04.024_bib24","unstructured":"U.S. Pharmacopeia General Chapters:51 Antimicrobial effectiveness testing. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011."},{"issue":"9","key":"10.1016\/j.jbiotec.2025.04.024_bib25","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1002\/jms.4424","article-title":"Characterization of stable and reactive metabolites of piperine formed on incubation with human liver microsomes","volume":"54","author":"Praneetha","year":"2019","journal-title":"J. Mass Spectrom."},{"issue":"2","key":"10.1016\/j.jbiotec.2025.04.024_bib26","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1158\/0008-5472.516.65.2","article-title":"Nitric oxide reverts the resistance to doxorubicin in human colon cancer cells by inhibiting the drug efflux","volume":"65","author":"Riganti","year":"2005","journal-title":"Cancer Res."},{"issue":"April","key":"10.1016\/j.jbiotec.2025.04.024_bib27","article-title":"Statistical evaluation of DPPH, ABTS, FRAP, and FOlin-ciocalteu Assays to Assess the Antioxidant Capacity of Lignins","volume":"233","author":"Rumpf","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"March","key":"10.1016\/j.jbiotec.2025.04.024_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jinorgbio.2012.11.007","article-title":"Hydroxylation of non-substituted polycyclic aromatic hydrocarbons by cytochrome P450 BM3 engineered by directed evolution","volume":"120","author":"Sideri","year":"2013","journal-title":"J. Inorg. Biochem."},{"issue":"10","key":"10.1016\/j.jbiotec.2025.04.024_bib29","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1016\/j.drudis.2020.07.015","article-title":"Liver says no: the ongoing search for safe catechol o-methyltransferase inhibitors to replace tolcapone","volume":"25","author":"Silva","year":"2020","journal-title":"Drug Discov. Today"},{"issue":"1","key":"10.1016\/j.jbiotec.2025.04.024_bib30","doi-asserted-by":"crossref","first-page":"7972","DOI":"10.1038\/s41598-017-08062-2","article-title":"Targeting P-glycoprotein: investigation of piperine analogs for overcoming drug resistance in cancer","volume":"7","author":"Syed","year":"2017","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.jbiotec.2025.04.024_bib31","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/cbdd.13758","article-title":"Overcoming vincristine resistance in cancer: computational design and discovery of piperine-inspired P-glycoprotein Inhibitors\u2019","volume":"97","author":"Syed","year":"2021","journal-title":"Chem. Biol. Drug Des."},{"key":"10.1016\/j.jbiotec.2025.04.024_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.medidd.2020.100027","article-title":"Piperine: a comprehensive review of methods of isolation, purification, and biological properties","volume":"7","author":"Tiwari","year":"2020","journal-title":"Med. Drug Discov."},{"issue":"12","key":"10.1016\/j.jbiotec.2025.04.024_bib33","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1002\/chem.201102470","article-title":"Identification of Mutant Asp251Gly\/Gln307His of Cytochrome P450 BM3 for the Generation of Metabolites of Diclofenac, Ibuprofen and Tolbutamide\u2019","volume":"18","author":"Tsotsou","year":"2012","journal-title":"Chem. - A Eur. J."},{"issue":"4","key":"10.1016\/j.jbiotec.2025.04.024_bib34","first-page":"6","article-title":"Development and validation of rapid RP- HPLC method for estimation of piperine In Piper nigrum L","volume":"1","author":"Upadhyay","year":"2013","journal-title":"Int. J. Herb. Med."},{"key":"10.1016\/j.jbiotec.2025.04.024_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejmech.2020.112385","article-title":"Identification and optimization of piperine analogues as neuroprotective agents for the treatment of Parkinson\u2019s disease via the activation of Nrf2\/Keap1 pathway","volume":"199","author":"Wang","year":"2020","journal-title":"Eur. J. Med. Chem."},{"issue":"3","key":"10.1016\/j.jbiotec.2025.04.024_bib36","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1039\/C1CS15192D","article-title":"P450 BM3 (CYP102A1): Connecting the Dots","volume":"41","author":"Whitehouse","year":"2012","journal-title":"Chem. Soc. Rev."},{"issue":"2","key":"10.1016\/j.jbiotec.2025.04.024_bib37","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.lwt.2012.07.036","article-title":"Antioxidant and antimicrobial activities of various solvent extracts, piperine and piperic acid from piper nigrum","volume":"50","author":"Zarai","year":"2013","journal-title":"LWT - Food Sci. Technol."},{"key":"10.1016\/j.jbiotec.2025.04.024_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioorg.2023.106589","article-title":"Synthesis and evaluation of piperine analogs as thioredoxin reductase inhibitors to cause oxidative stress-induced cancer cell apoptosis","volume":"138","author":"Zhong","year":"2023","journal-title":"Bioorg. Chem."}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165625001099?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165625001099?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T12:04:52Z","timestamp":1752581092000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165625001099"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":38,"alternative-id":["S0168165625001099"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2025.04.024","relation":{},"ISSN":["0168-1656"],"issn-type":[{"type":"print","value":"0168-1656"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biocatalytic production of a monoamine oxidase B\/catechol-O-methyltransferase inhibitor from piperine by engineered P450 BM3.","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2025.04.024","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}