{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T01:40:14Z","timestamp":1781055614010,"version":"3.54.1"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001858","name":"VINNOVA","doi-asserted-by":"publisher","award":["2022-03170"],"award-info":[{"award-number":["2022-03170"]}],"id":[{"id":"10.13039\/501100001858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001858","name":"VINNOVA","doi-asserted-by":"publisher","award":["2021-02640"],"award-info":[{"award-number":["2021-02640"]}],"id":[{"id":"10.13039\/501100001858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.jbiotec.2026.01.012","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T17:01:57Z","timestamp":1769533317000},"page":"89-101","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Efficiency and scalability in harvesting mammalian cell cultures: A scale-down approach to continuous centrifugation"],"prefix":"10.1016","volume":"411","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0182-8664","authenticated-orcid":false,"given":"Michaela","family":"D\u00f6lle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8343-1958","authenticated-orcid":false,"given":"Maxime","family":"Herv\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Staffan","family":"K\u00f6nigsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Thorwid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9977-5724","authenticated-orcid":false,"given":"Johan","family":"Rockberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Chotteau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.jbiotec.2026.01.012_b1","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1002\/btpr.1948","article-title":"A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies","volume":"30","author":"Aboulaich","year":"2014","journal-title":"Biotechnol. Prog."},{"issue":"5","key":"10.1016\/j.jbiotec.2026.01.012_b2","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1002\/jctb.7351","article-title":"Single-use centrifugal separator enables intensification of the clarification process in biomanufacturing of recombinant proteins","volume":"98","author":"Agarwal","year":"2023","journal-title":"J. Chem. Technol. Biotechnol."},{"issue":"2","key":"10.1016\/j.jbiotec.2026.01.012_b3","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/jbmte.390010206","article-title":"The theory of scaling up laboratory data for the sedimentation type centrifuge","volume":"1","author":"Ambler","year":"1959","journal-title":"J. Biochem. Microbiol. Technol. Eng."},{"key":"10.1016\/j.jbiotec.2026.01.012_b4","series-title":"Centrifugal Separations in Molecular and Cell Biology","author":"Birnie","year":"2014"},{"issue":"1","key":"10.1016\/j.jbiotec.2026.01.012_b5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF02474665","article-title":"Hemolytic effects of energy dissipation in flowing blood","volume":"7","author":"Bluestein","year":"1969","journal-title":"Med. Biol. Eng."},{"issue":"4","key":"10.1016\/j.jbiotec.2026.01.012_b6","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1007\/s00253-012-4451-z","article-title":"Recent advances in technology supporting biopharmaceutical production from mammalian cells","volume":"96","author":"Butler","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"6","key":"10.1016\/j.jbiotec.2026.01.012_b7","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.3109\/07388551.2015.1084266","article-title":"Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives","volume":"36","author":"Dumont","year":"2016","journal-title":"Crit. Rev. Biotechnol."},{"issue":"5","key":"10.1016\/j.jbiotec.2026.01.012_b8","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1002\/btpr.1776","article-title":"Process cost and facility considerations in the selection of primary cell culture clarification technology","volume":"29","author":"Felo","year":"2013","journal-title":"Biotechnol. Prog."},{"issue":"4","key":"10.1016\/j.jbiotec.2026.01.012_b9","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1002\/bit.22146","article-title":"Evaluation of the effect of chronic hydrodynamical stresses on cultures of suspensed CHO-6E6 cells","volume":"102","author":"Godoy-Silva","year":"2009","journal-title":"Biotechnol Bioeng"},{"issue":"1","key":"10.1016\/j.jbiotec.2026.01.012_b10","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/bit.24607","article-title":"The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process","volume":"110","author":"Hogwood","year":"2013","journal-title":"Biotechnol. Bioeng."},{"issue":"5","key":"10.1016\/j.jbiotec.2026.01.012_b11","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/s10616-011-9368-3","article-title":"The potential of hydrodynamic damage to animal cells of industrial relevance: current understanding","volume":"63","author":"Hu","year":"2011","journal-title":"Cytotechnology"},{"key":"10.1016\/j.jbiotec.2026.01.012_b12","series-title":"Development of a single-use hermetic centrifuge system","author":"Hundley","year":"2020"},{"issue":"3","key":"10.1016\/j.jbiotec.2026.01.012_b13","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/bit.21029","article-title":"Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification","volume":"95","author":"Hutchinson","year":"2006","journal-title":"Biotechnol. Bioeng."},{"issue":"4","key":"10.1016\/j.jbiotec.2026.01.012_b14","doi-asserted-by":"crossref","DOI":"10.1002\/btpr.3448","article-title":"Host cell proteins in monoclonal antibody processing: Control, detection, and removal","volume":"40","author":"Ito","year":"2024","journal-title":"Biotechnol. Prog."},{"issue":"9","key":"10.1016\/j.jbiotec.2026.01.012_b15","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.1002\/bit.25967","article-title":"A scale-down mimic for mapping the process performance of centrifugation, depth and sterile filtration","volume":"113","author":"Joseph","year":"2016","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jbiotec.2026.01.012_b16","series-title":"Biopharmaceutical Manufacturing: Progress, Trends and Challenges","first-page":"3","article-title":"Single-use systems in biopharmaceutical manufacture: State of the art and recent trends","author":"Jossen","year":"2023"},{"issue":"8","key":"10.1016\/j.jbiotec.2026.01.012_b17","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.tibtech.2013.05.011","article-title":"Continuous downstream processing of biopharmaceuticals","volume":"31","author":"Jungbauer","year":"2013","journal-title":"Trends Biotechnol."},{"issue":"3","key":"10.1016\/j.jbiotec.2026.01.012_b18","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1002\/bab.1617","article-title":"Progress in biopharmaceutical development","volume":"65","author":"Kesik-Brodacka","year":"2018","journal-title":"Biotechnol. Appl. Biochem."},{"issue":"3","key":"10.1016\/j.jbiotec.2026.01.012_b19","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1007\/s00253-011-3758-5","article-title":"CHO cells in biotechnology for production of recombinant proteins: current state and further potential","volume":"93","author":"Kim","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"11","key":"10.1016\/j.jbiotec.2026.01.012_b20","article-title":"Evaluation of single-use fluidized bed centrifuge system for mammalian cell harvesting","volume":"25","author":"Ko","year":"2012","journal-title":"BioPharm Int."},{"key":"10.1016\/j.jbiotec.2026.01.012_b21","series-title":"Centrifugal Separations in Biotechnology","author":"Leung","year":"2020"},{"issue":"1","key":"10.1016\/j.jbiotec.2026.01.012_b22","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/BF00369259","article-title":"The scale-down of an industrial disc stack centrifuge","volume":"8","author":"Mannweiler","year":"1992","journal-title":"Bioprocess Eng."},{"issue":"3","key":"10.1016\/j.jbiotec.2026.01.012_b23","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/(SICI)1097-0290(20000205)67:3<265::AID-BIT2>3.0.CO;2-J","article-title":"The use of laboratory centrifugation studies to predict performance of industrial machines: studies of shear-insensitive and shear-sensitive materials","volume":"67","author":"Maybury","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jbiotec.2026.01.012_b24","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2020.107552","article-title":"Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing","volume":"43","author":"O\u2019Flaherty","year":"2020","journal-title":"Biotech. Adv."},{"issue":"12","key":"10.1016\/j.jbiotec.2026.01.012_b25","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1002\/cite.202200096","article-title":"Qualification of a single-use disk stack separator for cell separation in mammalian cell-based antibody production","volume":"94","author":"Ott","year":"2022","journal-title":"Chem. Ing. Tech."},{"key":"10.1016\/j.jbiotec.2026.01.012_b26","doi-asserted-by":"crossref","first-page":"S82","DOI":"10.1016\/S0263-8762(97)80006-8","article-title":"Sedimentation and fluidisation: Part i","volume":"75","author":"Richardson","year":"1997","journal-title":"Chem. Eng. Res. Des."},{"issue":"3","key":"10.1016\/j.jbiotec.2026.01.012_b27","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1021\/bp070414x","article-title":"Advances in primary recovery: centrifugation and membrane technology","volume":"24","author":"Roush","year":"2008","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.jbiotec.2026.01.012_b28","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2020.107887","article-title":"A novel clarification approach for intensified monoclonal antibody processes with 100 million cells\/mL using a single-use fluidized bed centrifuge","volume":"167","author":"Saballus","year":"2021","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.jbiotec.2026.01.012_b29","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2022.108426","article-title":"Monitoring of amino acids and antibody N-glycosylation in high cell density perfusion culture based on Raman spectroscopy","volume":"182","author":"Schwarz","year":"2022","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.jbiotec.2026.01.012_b30","article-title":"Guidelines for an optimized differential centrifugation of cells","volume":"36","author":"Sharifian Gh","year":"2023","journal-title":"Biochem. Biophys. Rep."},{"issue":"12","key":"10.1016\/j.jbiotec.2026.01.012_b31","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1002\/bit.26812","article-title":"Progression of continuous downstream processing of monoclonal antibodies: Current trends and challenges","volume":"115","author":"Somasundaram","year":"2018","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jbiotec.2026.01.012_b32","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ddtec.2021.02.003","article-title":"Recent advances in CHO cell line development for recombinant protein production","volume":"38","author":"Tihanyi","year":"2020","journal-title":"Drug Discov. Today: Technol."},{"issue":"5","key":"10.1016\/j.jbiotec.2026.01.012_b33","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1002\/bit.23051","article-title":"Modeling industrial centrifugation of mammalian cell culture using a capillary based scale-down system","volume":"108","author":"Westoby","year":"2011","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jbiotec.2026.01.012_b34","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ymben.2020.11.004","article-title":"Control of IgG glycosylation in CHO cell perfusion cultures by GReBA mathematical model supported by a novel targeted feed, TAFE","volume":"65","author":"Zhang","year":"2021","journal-title":"Metab. Eng."}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165626000209?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165626000209?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T14:38:56Z","timestamp":1778510336000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165626000209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":34,"alternative-id":["S0168165626000209"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2026.01.012","relation":{},"ISSN":["0168-1656"],"issn-type":[{"value":"0168-1656","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficiency and scalability in harvesting mammalian cell cultures: A scale-down approach to continuous centrifugation","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2026.01.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}