{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:48:37Z","timestamp":1792586917846,"version":"4.5.0"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402063"],"award-info":[{"award-number":["82402063"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M761485"],"award-info":[{"award-number":["2024M761485"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biotechnology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.jbiotec.2026.10.007","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:00:01Z","timestamp":1791475201000},"page":"301-309","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["One-step purification of transglutaminase with high purity using a thermoseparating ethylene oxide\/propylene oxide copolymer"],"prefix":"10.1016","volume":"420","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jbiotec.2026.10.007_bib1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.tifs.2021.02.036","article-title":"Recent advances in microbial transglutaminase biosynthesis and its application in the food industry","volume":"110","author":"Akbari","year":"2021","journal-title":"Trends Food Sci. & Technol."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib2","doi-asserted-by":"crossref","first-page":"10269","DOI":"10.1021\/j100142a004","article-title":"A well-behaved electrostatic potential based method using charge restraints for deriving atomic charges: the RESP model","volume":"97","author":"Bayly","year":"1993","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib3","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh Ewald: An N\u22c5log(N) method for Ewald sums in large systems","volume":"98","author":"Darden","year":"1993","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0300-9084(98)80073-4","article-title":"Purification, characterisation, and gene cloning of transglutaminase from Streptoverticillium cinnamoneum CBS 683.68","volume":"80","author":"Duran","year":"1998","journal-title":"Biochimie"},{"key":"10.1016\/j.jbiotec.2026.10.007_bib5","doi-asserted-by":"crossref","first-page":"31339","DOI":"10.1039\/D1RA05817G","article-title":"Investigating the structure-activity relationship of marine natural polyketides as promising SARS-CoV-2 main protease inhibitors","volume":"11","author":"El-Demerdash","year":"2021","journal-title":"RSC Adv."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib6","doi-asserted-by":"crossref","first-page":"4872","DOI":"10.1093\/nar\/gky221","article-title":"Influence of Na+ and Mg2+ ions on RNA structures studied with molecular dynamics simulations","volume":"46","author":"Fischer","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib7","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1146\/annurev.bi.49.070180.002505","article-title":"Transglutaminases","volume":"49","author":"Folk","year":"1980","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib8","doi-asserted-by":"crossref","first-page":"5518","DOI":"10.1016\/S0021-9258(18)96373-8","article-title":"Mechanism of action of guinea pig liver transglutaminase. I. Purification and properties of the enzyme: identification of a functional cysteine essential for activity","volume":"241","author":"Folk","year":"1966","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib9","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0021-9258(19)50936-X","article-title":"The enzymatic formation of hydroxamic acids from glutamine and asparagine","volume":"187","author":"Grossowicz","year":"1950","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib10","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-025-03021-8","article-title":"Polyoxyethylene and polypropylene emulsification enhances retinol palmitate efficacy in corneal wound healing","volume":"15","author":"Horiuchi","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib11","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/s42003-023-05175-5","article-title":"Magnesium ions mediate ligand binding and conformational transition of the SAM\/SAH riboswitch","volume":"6","author":"Hu","year":"2023","journal-title":"Commun. Biol."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib12","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.molcatb.2016.07.005","article-title":"Purification and characterization of a high-salt-resistant microbial transglutaminase from Streptomyces mobaraensis","volume":"133","author":"Jin","year":"2016","journal-title":"J. Mol. Catal. B Enzym."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib13","doi-asserted-by":"crossref","first-page":"44252","DOI":"10.1074\/jbc.M203933200","article-title":"Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense","volume":"277","author":"Kashiwagi","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib14","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s00253-025-13697-7","article-title":"Microbial transglutaminase in food biotechnology: from biochemical mechanisms to industrial applications","volume":"110","author":"Kieliszek","year":"2026","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib15","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s12223-013-0287-x","article-title":"Microbial transglutaminase and its application in the food industry. A review","volume":"59","author":"Kieliszek","year":"2014","journal-title":"Folia Microbiol."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib16","article-title":"Microbiological transglutaminase: Biotechnological application in the food industry","volume":"18","author":"Kolotylo","year":"2023"},{"key":"10.1016\/j.jbiotec.2026.10.007_bib17","doi-asserted-by":"crossref","DOI":"10.1186\/s12896-016-0304-7","article-title":"Improving the active expression of transglutaminase in Streptomyces lividans by promoter engineering and codon optimization","volume":"16","author":"Liu","year":"2016","journal-title":"BMC Biotech."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib18","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/j.1574-6968.2011.02387.x","article-title":"The pro-region of Streptomyces hygroscopicus transglutaminase affects its secretion by Escherichia coli","volume":"324","author":"Liu","year":"2011","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib19","doi-asserted-by":"crossref","first-page":"6627","DOI":"10.1016\/j.chroma.2009.07.067","article-title":"Characterization of ethylene oxide-propylene oxide block copolymers by combination of different chromatographic techniques and matrix-assisted laser desorption ionization time-of-flight mass spectroscopy","volume":"1216","author":"Malik","year":"2009","journal-title":"J. Chromatogr. A."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib20","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1080\/10242422.2020.1720660","article-title":"Microbial transglutaminase: An overview of recent applications in food and packaging","volume":"38","author":"Mostafa","year":"2020","journal-title":"Biocatal. Biotransform."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib21","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202501008","article-title":"A Novel Injectable Composite Hydrogel HAp-GelMA Co-Doped Magnesium\/Zinc Promotes Bone Generation and Angiogenesis Synergistically","volume":"14","author":"Qiang","year":"2025","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib22","doi-asserted-by":"crossref","first-page":"6881","DOI":"10.1021\/acs.chemrev.6b00652","article-title":"Temperature-Responsive Ionic Liquids: Fundamental Behaviors and Catalytic Applications","volume":"117","author":"Qiao","year":"2017","journal-title":"Chem. Rev."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib23","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.seppur.2005.09.017","article-title":"Three-liquid-phase extraction systems for separation of phenol and p-nitrophenol from wastewater","volume":"49","author":"Shen","year":"2006","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib24","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.jmgm.2005.12.005","article-title":"Automatic atom type and bond type perception in molecular mechanical calculations","volume":"25","author":"Wang","year":"2006","journal-title":"J. Mol. Graph. Modell."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib25","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1002\/jcc.20035","article-title":"Development and testing of a general amber force field","volume":"25","author":"Wang","year":"2004","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib26","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1002\/jssc.202100580","article-title":"Effective extraction of tylosin and spiramycin from fermentation broth using thermo-responsive ethylene oxide\/propylene oxide aqueous two-phase systems","volume":"45","author":"Yang","year":"2022","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib27","doi-asserted-by":"crossref","first-page":"3559","DOI":"10.3168\/jds.2011-5125","article-title":"Enzymatic characterization of transglutaminase from Streptomyces mobaraensis DSM 40587 in high salt and effect of enzymatic cross-linking of yak milk proteins on functional properties of stirred yogurt","volume":"95","author":"Zhang","year":"2012","journal-title":"J. Dairy. Sci."},{"key":"10.1016\/j.jbiotec.2026.10.007_bib28","first-page":"240","article-title":"Effects of hydrophobicity of ethylene oxide-propylene oxide copolymers on phase diagrams of aqueous two-phase systems and partition behaviors of cephalexin and 7-aminodeacetoxicephalos-poranic acid","volume":"10","author":"Zhu","year":"2002","journal-title":"Chin. J. Chem. Eng."}],"container-title":["Journal of Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165626002749?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168165626002749?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:30:41Z","timestamp":1792585841000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168165626002749"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":28,"alternative-id":["S0168165626002749"],"URL":"https:\/\/doi.org\/10.1016\/j.jbiotec.2026.10.007","relation":{},"ISSN":["0168-1656"],"issn-type":[{"value":"0168-1656","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"One-step purification of transglutaminase with high purity using a thermoseparating ethylene oxide\/propylene oxide copolymer","name":"articletitle","label":"Article Title"},{"value":"Journal of Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jbiotec.2026.10.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}