{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:34:18Z","timestamp":1789752858265,"version":"4.0.1"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2017,6]]},"DOI":"10.1016\/j.jchromb.2017.04.009","type":"journal-article","created":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T20:24:26Z","timestamp":1491337466000},"page":"80-92","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"special_numbering":"C","title":["The combined use of analytical tools for exploring tetanus toxin and tetanus toxoid structures"],"prefix":"10.1016","volume":"1054","author":[{"given":"Caroline","family":"Bayart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Peronin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Jean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Paladino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Talaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc Le","family":"Borgne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2017.04.009_bib0005","doi-asserted-by":"publisher","unstructured":"Global, regional, and national age\u2013sex specific all-cause and cause-specific mortality for 240 causes of death, 1990\u20132013: a systematic analysis for the Global Burden of Disease Study 2013, The Lancet. 385 (2015) 117-171. 10.1016\/S0140-6736(14)61682-2.","DOI":"10.1016\/S0140-6736(14)61682-2"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0010","doi-asserted-by":"publisher","first-page":"8889","DOI":"10.1074\/jbc.275.12.8889","article-title":"The Structures of the HC Fragment of Tetanus Toxin with Carbohydrate Subunit Complexes Provide Insight into Ganglioside Binding","volume":"275","author":"Emsley","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0015","doi-asserted-by":"publisher","first-page":"25173","DOI":"10.1074\/jbc.274.35.25173","article-title":"Neuronal Sensitivity to Tetanus Toxin Requires Gangliosides","volume":"274","author":"Williamson","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0020","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1002\/j.1460-2075.1986.tb04527.x","article-title":"Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins","volume":"5","author":"Eisel","year":"1986","journal-title":"EMBO J."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0025","doi-asserted-by":"crossref","first-page":"3588","DOI":"10.1128\/IAI.57.11.3588-3593.1989","article-title":"Isolation purification, and characterization of fragment B, the NH2-terminal half of the heavy chain of tetanus toxin","volume":"57","author":"Matsuda","year":"1989","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0030","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1365-2958.1994.tb00396.x","article-title":"Mechanism of action of tetanus and botulinum neurotoxins","volume":"13","author":"Montecucco","year":"1994","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0035","doi-asserted-by":"publisher","first-page":"2622","DOI":"10.3390\/toxins2112622","article-title":"Tetanus toxin C-fragment: the courier and the cure?","volume":"2","author":"Toivonen","year":"2010","journal-title":"Toxins."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0040","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.toxicon.2013.08.055","article-title":"Engineering Clostridia Neurotoxins with elevated catalytic activity","volume":"74","author":"Guo","year":"2013","journal-title":"Toxicon Off J. Int. Soc. Toxinol."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0045","first-page":"1045","article-title":"The design of semi-synthetic and synthetic glycoconjugate vaccines, Expert Opin","volume":"6","author":"Costantino","year":"2011","journal-title":"Drug Discov."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0050","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1046\/j.1365-2249.2000.01109.x","article-title":"Conjugate vaccines","volume":"119","author":"Goldblatt","year":"2000","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0055","doi-asserted-by":"publisher","first-page":"2213","DOI":"10.1016\/j.vaccine.2006.12.033","article-title":"Investigation of the detoxification mechanism of formaldehyde-treated tetanus toxin","volume":"25","author":"Thaysen-Andersen","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0060","doi-asserted-by":"publisher","first-page":"6235","DOI":"10.1074\/jbc.M310752200","article-title":"Identification of formaldehyde-induced modifications in proteins: reactions with model peptides","volume":"279","author":"Metz","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0065","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1021\/bc050340f","article-title":"Identification of formaldehyde-induced modifications in proteins: reactions with insulin","volume":"17","author":"Metz","year":"2006","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0070","series-title":"Guanidino Compd. Biol. Med.","first-page":"167","article-title":"Antagonistic reactions of arginine and lysine against formaldehyde and their relation to cell proliferation, apoptosis, folate cycle and photosynthesis","author":"Tr\u00e9zl","year":"2003"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0075","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1042\/bj0380309","article-title":"The reaction between histidine and formaldehyde","volume":"38","author":"Neuberger","year":"1944","journal-title":"Biochem. J."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0080","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1021\/ja01222a023","article-title":"The Reaction of Formaldehyde with Proteins","volume":"67","author":"Fraenkel-Conrat","year":"1945","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0085","doi-asserted-by":"crossref","first-page":"5851","DOI":"10.1016\/S0021-9258(18)96350-7","article-title":"The Mechanism of the condensation of formaldehyde with tetrahydrofolic acid","volume":"241","author":"Kallen","year":"1966","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0090","first-page":"231","article-title":"Physicochemical and immunochemical assays for monitoring consistent production of tetanus toxoid","volume":"41","author":"Metz","year":"2013","journal-title":"Biol. J. Int. Assoc. Biol. Stand."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0095","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1016\/j.vaccine.2015.01.046","article-title":"Structural correlates of carrier protein recognition in tetanus toxoid-conjugated bacterial polysaccharide vaccines","volume":"33","author":"Lockyer","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0100","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1515\/znc-2004-11-1222","article-title":"An improved micromethod for tyrosine estimation","volume":"59","author":"Acharya","year":"2004","journal-title":"Z. Naturforschung C J. Biosci."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0105","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/0003-2697(79)90792-9","article-title":"Ellman\u2019s reagent: 5, 5\u2032-dithiobis(2-nitrobenzoic acid)\u2013a reexamination","volume":"94","author":"Riddles","year":"1979","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0110","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1016\/0003-2697(66)90275-2","article-title":"Determination of free amino groups in proteins by trinitrobenzenesulfonic acid","volume":"14","author":"Habeeb","year":"1966","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0115","series-title":"Curr Protoc. Protein Sci.","article-title":"Amino Acid Analysis","author":"Rutherfurd","year":"2001"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0120","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein Measurement with the Folin Phenol Reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0125","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.chroma.2007.06.063","article-title":"Analysis of post-translational modifications in recombinant monoclonal antibody IgG1 by reversed-phase liquid chromatography\/mass spectrometry","author":"Yan","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0130","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/S0021-9673(98)00721-3","article-title":"Hydrolysis and amino acid composition analysis of proteins","volume":"826","author":"Fountoulakis","year":"1998","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0135","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/S0076-6879(83)91007-8","article-title":"Single hydrolysis method for all amino acids, including cysteine and tryptophan","volume":"91","author":"Inglis","year":"1983","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0140","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/0003-2697(89)90612-X","article-title":"Hydrolysis of proteins and peptides in a hermetically sealed microcapillary tube: High recovery of labile amino acids","volume":"182","author":"Liu","year":"1989","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0145","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1021\/jf60176a010","article-title":"Methods of tryptophan analysis","volume":"19","author":"Friedman","year":"1971","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0150","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/S0021-9258(18)72759-2","article-title":"A New and Highly Specific Colorimetric Test for Methionine","volume":"141","author":"McCarthy","year":"1941","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0155","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/j.tibtech.2005.07.008","article-title":"Field-flow fractionation and biotechnology","volume":"23","author":"Reschiglian","year":"2005","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0160","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1002\/(SICI)1097-0282(199812)46:7<489::AID-BIP6>3.0.CO;2-E","article-title":"Buffer dependence of refractive index increments of protein solutions","volume":"46","author":"Ball","year":"1998","journal-title":"Biopolymers"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0165","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0003-2670(93)80373-S","article-title":"Light scattering and the absolute characterization of macromolecules","volume":"272","author":"Wyatt","year":"1993","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jchromb.2017.04.009_bib0170","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1016\/j.toxicon.2005.02.032","article-title":"Structural analysis of the catalytic domain of tetanus neurotoxin, Toxicon Off","volume":"45","author":"Rao","year":"2005","journal-title":"J. Int. Soc. Toxinol."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0175","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.1021\/js960148+","article-title":"The secondary structure and aggregation of lyophilized tetanus toxoid","volume":"85","author":"Costantino","year":"1996","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.jchromb.2017.04.009_bib0180","first-page":"1722","article-title":"Reversible transition between alpha-helix and beta-sheet conformation of a transmembrane domain","volume":"2009","author":"Yassine","year":"1788","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023217301642?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023217301642?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T05:48:30Z","timestamp":1658900910000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023217301642"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":36,"alternative-id":["S1570023217301642"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2017.04.009","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2017,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The combined use of analytical tools for exploring tetanus toxin and tetanus toxoid structures","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2017.04.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}