{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:06:33Z","timestamp":1790971593596,"version":"4.1.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81373973"],"award-info":[{"award-number":["81373973"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573872"],"award-info":[{"award-number":["81573872"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010618","name":"Guangzhou University of Chinese Medicine","doi-asserted-by":"publisher","award":["XH20170108"],"award-info":[{"award-number":["XH20170108"]}],"id":[{"id":"10.13039\/501100010618","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Major program for the fundamental research of the education department of Guangdong province","award":["2017KZDXM018"],"award-info":[{"award-number":["2017KZDXM018"]}]},{"name":"Guangdong Provincial Science and Technology projects","award":["2013B032500015"],"award-info":[{"award-number":["2013B032500015"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1016\/j.jchromb.2018.06.027","type":"journal-article","created":{"date-parts":[[2018,6,18]],"date-time":"2018-06-18T19:17:55Z","timestamp":1529349475000},"page":"320-327","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Identification of active ingredients mediating anti-platelet aggregation effects of BuyangHuanwu decoction using a platelet binding assay, solid phase extraction, and HPLC-MS\/MS"],"prefix":"10.1016","volume":"1092","author":[{"given":"Fengyun","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiming","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianye","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinai","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huazhu","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjiao","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2018.06.027_bb0005","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.jpba.2016.06.030","article-title":"Microdialysis combined with UPLC-MS\/MS method for determination of tetramethylpyrazine and ferulic acid in striatum of awake and anesthetic rats subjected to cerebral ischemia","volume":"128","author":"Liao","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0010","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jpba.2013.07.046","article-title":"Effect of BuyangHuanwu decoction on amino acid content in cerebrospinal fluid of rats during ischemic\/reperfusion injury","volume":"86","author":"Wang","year":"2013","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0015","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1186\/s12967-015-0630-x","article-title":"Platelets, gender and acute cerebral infarction","volume":"13","author":"J\u00e4remo","year":"2015","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0020","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1016\/j.jstrokecerebrovasdis.2015.06.018","article-title":"The role of circulating platelets microparticles and platelet parameters in acute ischemic stroke patients","volume":"24","author":"Chen","year":"2015","journal-title":"J. Stroke Cerebrovasc. Dis."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0025","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1177\/1076029616660762","article-title":"Antiplatelet and anticoagulant therapies for prevention of ischemic stroke","volume":"23","author":"Kapil","year":"2017","journal-title":"Clin. Appl. Thromb. Hemost."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0030","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1186\/1472-6882-13-309","article-title":"Neuroprotective effect of BuyangHuanwu Decoction on spinal ischemia-reperfusion injury in rats is linked with inhibition of cyclin-dependent kinase 5","volume":"13","author":"Wang","year":"2013","journal-title":"BMC Complement. Altern. Med."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0035","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.4103\/1673-5374.141791","article-title":"Neuroprotective effects of BuyangHuanwu decoction on cerebral ischemia-induced neuronal damage","volume":"9","author":"Mu","year":"2014","journal-title":"Neural Regen. Res."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0040","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.jep.2011.05.008","article-title":"The effects of BuyangHuanwu Decoction on hemorheological disorders and energy metabolism in rats with coronary heart disease","volume":"137","author":"Wang","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0045","first-page":"299","article-title":"Effect of BuyangHuanwu decoction on platelet activating factor content in arterial blood pre- and post-arterial thrombosis in rats","volume":"21","author":"Zhang","year":"2001","journal-title":"J. Tradit. Chin. Med."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0050","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/138484","article-title":"A systematic review and meta-analysis of BuyangHuanwu decoction in animal model of focal cerebral ischemia","volume":"2013","author":"Wei","year":"2013","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0055","first-page":"236","article-title":"Effects of four active parts of BuyangHuanwu Decoction on cerebral infarct volume in rats with focal cerebral ischemia","volume":"36","author":"Tang","year":"2005","journal-title":"Chin. Herb. Med."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0060","doi-asserted-by":"crossref","first-page":"532","DOI":"10.4314\/ajtcam.v10i6.24","article-title":"A review of the pharmacological mechanism of traditional Chinese medicine in the intervention of coronary heart disease and stroke","volume":"10","author":"Zhang","year":"2013","journal-title":"Afr. J. Tradit. Complement. Altern. Med."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0065","doi-asserted-by":"crossref","first-page":"3351","DOI":"10.1039\/c2ay25703c","article-title":"Screening active components acting on a1A adrenergic receptors from agrimony using a Sprague-Dawley rat prostate cell membrane chromatography online coupled HPLC\/MS method","volume":"4","author":"Han","year":"2012","journal-title":"Anal. Methods"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0070","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.jpba.2013.04.001","article-title":"Screening of target compounds from FructusPiperis using high \u03b1\u2081A adrenoreceptor expression cell membrane chromatography online coupled with high performance liquid chromatography tandem mass spectrometry","volume":"81-82","author":"Han","year":"2013","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"2\u20133","key":"10.1016\/j.jchromb.2018.06.027_bb0075","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1007\/s00216-012-6353-4","article-title":"Development of new chromatographic tools based on A2A adenosine receptor subtype for ligand characterization and screening by FAC-MS","volume":"405","author":"Temporini","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0080","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.talanta.2017.11.018","article-title":"Biospecific isolation and characterization of angiogenesis-promoting ingredients in Buyang Huanwu decoction using affinity chromatography on rat brain microvascular endothelial cells combined with solid-phase extraction, and HPLC-MS\/MS","volume":"179","author":"Liao","year":"2018","journal-title":"Talanta"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0085","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.jpba.2005.02.008","article-title":"Hypothesis of potential active components in Angelica sinensis by using biomembrane extraction and high performance liquid chromatography","volume":"38","author":"Dong","year":"2005","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0090","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.jpba.2006.01.014","article-title":"Live cell extraction and HPLC-MS analysis for predicting bioactive components of traditional Chinese medicines","volume":"41","author":"Li","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0095","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1002\/jssc.200700507","article-title":"Screening of anti-platelet aggregation agents from Panaxnotoginseng using human platelet extraction and HPLC-DAD-ESI-MS\/MS","volume":"31","author":"Wang","year":"2008","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0100","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.jpba.2015.06.009","article-title":"A Platelet\/CMC coupled with offline UPLC-QTOF-MS\/MS for screening antiplatelet activity components from aqueous extract of Danshen","volume":"117","author":"Chen","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0105","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1002\/pca.1260","article-title":"Analysis of marker compounds with anti-platelet aggregation effects in Mailuoning injection using platelet binding assay combined with HPLC-DAD-ESI-MS and solid-phase extraction technique","volume":"22","author":"Yu","year":"2011","journal-title":"Phytochem. Anal."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0110","series-title":"Reference Manual for Blood Physiology and Biochemistry of Experimental Animals","author":"Wang","year":"2011"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0115","first-page":"27","article-title":"Determination of physiological indexes of blood in pigs","volume":"5","author":"Liang","year":"2011","journal-title":"Chin. Pig Ind."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0120","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1046\/j.1538-7836.2003.00457.x","article-title":"Differences and similarities in tyrosine phosphorylation of proteins in platelets from human and pig species","volume":"1","author":"Zurbano","year":"2003","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0125","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.2147\/NDT.S111108","article-title":"Mean platelet volume and red cell distribution width levels in initial evaluation of panic disorder","volume":"12","author":"Asoglu","year":"2016","journal-title":"Neuropsychiatr. Dis. Treat."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0130","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.fitote.2016.11.016","article-title":"Curdione attenuates thrombin-induced human platelet activation: \u03b21-tubulin as a potential therapeutic target","volume":"116","author":"Zhang","year":"2017","journal-title":"Fitoterapia"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0135","doi-asserted-by":"crossref","first-page":"6591717","DOI":"10.1155\/2016\/6591717","article-title":"Platelet-Rich Plasma: the choice of activation method affects the release of bioactive molecule","volume":"2016","author":"Cavallo","year":"2016","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0140","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0123597","article-title":"Metabolic plasticity in resting and thrombin activated platelets","volume":"10","author":"Ravi","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0145","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1002\/bmc.1193","article-title":"Rapid separation and identification of 54 major constituents in BuyangHuanwu decoction by ultra-fast HPLC system coupled with DAD-TOF\/MS","volume":"23","author":"Liu","year":"2009","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0150","first-page":"333","article-title":"Study on flavonoids in the flower of Carthamus tinctorius L.","volume":"46","author":"F","year":"2011","journal-title":"Chin. Pharm. J."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0155","doi-asserted-by":"crossref","first-page":"7523","DOI":"10.1007\/s00216-013-7182-9","volume":"405","author":"Um","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0160","first-page":"2601","article-title":"Chemical constituents in roots of Astragalus membranaceus","volume":"48","author":"Li","year":"2017","journal-title":"Chin. Tradit. Herb. Drug"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0165","first-page":"661","article-title":"Studies on chemical constituents of Paeonia veitchii L","volume":"42","author":"Wang","year":"2007","journal-title":"Chin. Pharm. J."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0170","doi-asserted-by":"crossref","first-page":"3246","DOI":"10.1161\/STROKEAHA.113.002565","article-title":"Antiplatelet loading improves behavioral outcome in a rabbit model of stroke","volume":"44","author":"Meyer","year":"2013","journal-title":"Stroke"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0175","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1111\/jth.13377","article-title":"A rabbit model of cerebral microembolic signals for translational research: preclinical validation for aspirin and clopidogrel","volume":"14","author":"Zhou","year":"2016","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0180","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0167-5273(92)90106-D","article-title":"The effect of antiplatelet therapy on platelet accumulation after experimental angioplasty in the rabbit iliac model","volume":"36","author":"Faxon","year":"1992","journal-title":"Int. J. Cardiol."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0185","first-page":"4418","article-title":"Mechanism study on preventive and curative effects of BuyangHuanwu decoction in Qi deficiency and blood stasis diseases based on network analysis","volume":"39","author":"Ding","year":"2014","journal-title":"Zhongguo Zhong Yao ZaZhi"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0190","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jns.2014.06.033","article-title":"Formononetin mediates neuroprotection against cerebral ischemia\/reperfusion in rats via down regulation of the Bax\/Bcl-2 ratio and upregulation PI3K\/Akt signaling pathway","volume":"344","author":"Liang","year":"2014","journal-title":"J. Neurol. Sci."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0195","first-page":"1355","article-title":"Antithrombotic activity of Formononetin sodium and its mechanism","volume":"25","author":"Wan","year":"2016","journal-title":"Chin. J. New Drugs"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0200","first-page":"624","article-title":"Platelet anti-aggregatory and blood anti-coagulant effects of compounds isolated from Paeonialactiflora and Paeoniasuffruticosa","volume":"65","author":"Koo","year":"2010","journal-title":"Pharmazie"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0205","doi-asserted-by":"crossref","first-page":"3335","DOI":"10.1002\/jssc.201000340","article-title":"Roles of paeoniflorin and senkyunolide I in SiWu decoction on antiplatelet and anticoagulation activities","volume":"33","author":"Zhu","year":"2010","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jchromb.2018.06.027_bb0210","series-title":"A Dictionary of the Traditional Chinese Medicines","first-page":"992","author":"Jiangsu New Medical College","year":"1986"},{"key":"10.1016\/j.jchromb.2018.06.027_bb0215","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.jep.2016.06.009","article-title":"Effects of extracts and isolated compounds from safflower on some index of promoting blood circulation and regulating menstruation","volume":"191","author":"Yao","year":"2016","journal-title":"J. Ethnopharmacol."}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023217319396?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023217319396?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,8]],"date-time":"2019-08-08T18:40:44Z","timestamp":1565289644000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023217319396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":43,"alternative-id":["S1570023217319396"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2018.06.027","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of active ingredients mediating anti-platelet aggregation effects of BuyangHuanwu decoction using a platelet binding assay, solid phase extraction, and HPLC-MS\/MS","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2018.06.027","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}