{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T11:48:17Z","timestamp":1793620097905,"version":"4.5.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100005363","name":"Universidad de Buenos Aires","doi-asserted-by":"publisher","award":["20020170100030BA"],"award-info":[{"award-number":["20020170100030BA"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 11220130100119CO"],"award-info":[{"award-number":["PIP 11220130100119CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003033","name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n Productiva","doi-asserted-by":"publisher","award":["PICT\u20102016\u20100751"],"award-info":[{"award-number":["PICT\u20102016\u20100751"]}],"id":[{"id":"10.13039\/501100003033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.jchromb.2020.122070","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T14:10:16Z","timestamp":1584367816000},"page":"122070","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Use of a phosphopeptide as a ligand to purify phospholipase A2 from the venom of Crotalus durisuss terrificus by affinity chromatography"],"prefix":"10.1016","volume":"1146","author":[{"given":"Soledad L.","family":"Saavedra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"Acosta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda","family":"\u00c1vila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana L.","family":"Giudicessi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia A.","family":"Camperi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Albericio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osvaldo","family":"Cascone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda C.","family":"Mart\u00ednez Ceron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2020.122070_b0005","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1042\/bj0321375","article-title":"The molecular weight of crotoxin","volume":"32","author":"Gral\u00e9n","year":"1938","journal-title":"Biochem. J."},{"key":"10.1016\/j.jchromb.2020.122070_b0010","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.toxicon.2018.10.306","article-title":"Crotoxin from Crotalus durissus terrificus venom: In vitro cytotoxic activity of a heterodimeric phospholipase A2 on human cancer-derived cell lines","volume":"156","author":"Muller","year":"2018","journal-title":"Toxicon."},{"key":"10.1016\/j.jchromb.2020.122070_b0015","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/142213a0","article-title":"Two active proteins from rattlesnake venom [9]","volume":"142","author":"Slotta","year":"1938","journal-title":"Nature"},{"key":"10.1016\/j.jchromb.2020.122070_b0020","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1432-1033.1994.tb18978.x","article-title":"The origin of the diversity of crotoxin isoforms in the venom of Crotalus durissus terrificus","volume":"223","author":"Faure","year":"1994","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jchromb.2020.122070_b0025","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0041-0101(78)90056-9","article-title":"The crotoxin complex\u2014an example of biochemical and pharmacological protein complementation","volume":"16","author":"Habermann","year":"1978","journal-title":"Toxicon."},{"key":"10.1016\/j.jchromb.2020.122070_b0030","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s10930-005-6718-z","article-title":"Biochemical, pharmacological and structural characterization of a new PLA2 from Crotalus durissus terrificus (South American Rattlesnake) venom","volume":"24","author":"Hernandez-Oliveira","year":"2005","journal-title":"Protein J."},{"key":"10.1016\/j.jchromb.2020.122070_b0035","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.toxicon.2011.05.021","article-title":"Crotoxin and phospholipases A2 from Crotalus durissus terrificus showed antiviral activity against dengue and yellow fever viruses","volume":"59","author":"Muller","year":"2012","journal-title":"Toxicon."},{"key":"10.1016\/j.jchromb.2020.122070_b0040","doi-asserted-by":"crossref","first-page":"e112351","DOI":"10.1371\/journal.pone.0112351","article-title":"Phospholipase A2 isolated from the venom of Crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope","volume":"9","author":"Muller","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jchromb.2020.122070_b0045","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1007\/s00705-019-04172-6","article-title":"Expression, purification and virucidal activity of two recombinant isoforms of phospholipase A 2 from Crotalus durissus terrificus venom","volume":"164","author":"Russo","year":"2019","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jchromb.2020.122070_b0050","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/ddr.21456","article-title":"Harnessing snake venom phospholipases A 2 to novel approaches for overcoming antibiotic resistance","volume":"80","author":"Almeida","year":"2019","journal-title":"Drug Dev. Res."},{"key":"10.1016\/j.jchromb.2020.122070_b0055","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1186\/1471-2334-6-100","article-title":"In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei","volume":"6","author":"Perumal Samy","year":"2006","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.jchromb.2020.122070_b0060","doi-asserted-by":"crossref","first-page":"6150","DOI":"10.2174\/0929867311209066150","article-title":"Snake venom phospholipases A(2): a novel tool against bacterial diseases","volume":"19","author":"Samy","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.jchromb.2020.122070_b0065","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.bcp.2017.03.005","article-title":"Animal venoms as antimicrobial agents","volume":"134","author":"Samy","year":"2017","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.jchromb.2020.122070_b0070","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1021\/bi00402a036","article-title":"Crotoxin, a phospholipase A2 neurotoxin from the South American rattlesnake Crotalus durissus terrificus: purification of several isoforms and comparison of their molecular structure and of their biological activities","volume":"27","author":"Faure","year":"1988","journal-title":"Biochemistry"},{"key":"10.1016\/j.jchromb.2020.122070_b0075","doi-asserted-by":"crossref","first-page":"2540","DOI":"10.2174\/156802611797633384","article-title":"Therapeutic potential of peptides with neutralizing ability towards the venom and toxin (CaTx-I) of Crotalus adamanteus","volume":"11","author":"Samy","year":"2011","journal-title":"Curr. Top. Med. Chem."},{"key":"10.1016\/j.jchromb.2020.122070_b0080","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1002\/cpch.2","article-title":"Combinatorial library screening coupled to mass spectrometry to identify valuable cyclic peptides","volume":"8","author":"Camperi","year":"2016","journal-title":"Curr. Protoc. Chem. Biol."},{"key":"10.1016\/j.jchromb.2020.122070_b0085","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.2174\/1381612824666180223172854","article-title":"Natural snake venom inhibitors and their pharmaceutical uses: challenges and possibilities","volume":"24","author":"Saavedra","year":"2018","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.jchromb.2020.122070_b0090","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1002\/btpr.2645","article-title":"Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography","volume":"34","author":"Saavedra","year":"2018","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.jchromb.2020.122070_b0095","doi-asserted-by":"crossref","first-page":"e3128","DOI":"10.1002\/psc.3128","article-title":"Recombinant human follicle stimulating hormone purification by a short peptide affinity chromatography","author":"Gurevich Messina","year":"2018","journal-title":"J. Pept. Sci. 24"},{"key":"10.1016\/j.jchromb.2020.122070_b0100","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1021\/acs.bioconjchem.8b00395","article-title":"Development and characterization of peptide ligands of immunoglobulin G Fc region","volume":"29","author":"Kruljec","year":"2018","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.jchromb.2020.122070_b0105","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20010161","article-title":"Optimization of sequence, display, and mode of operation of IgG-binding peptide ligands to develop robust, high-capacity affinity adsorbents that afford high IgG product quality","volume":"20","author":"Islam","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jchromb.2020.122070_b0110","doi-asserted-by":"crossref","unstructured":"M.C. Mart\u00ednez-Ceron, S.L. Saavedra, L. \u00c1vila, S.L. Giudicessi, F. Albericio, S.A. Camperi, O. Cascone, One-Bead-One-Peptide Library to Purify Crotalus durissus terrificus Phosholipase A2, (2015). https:\/\/doi.org\/10.17952\/24APS.2015.137.","DOI":"10.17952\/24APS.2015.137"},{"key":"10.1016\/j.jchromb.2020.122070_b0115","doi-asserted-by":"crossref","first-page":"4085","DOI":"10.1021\/acsnano.5b00133","article-title":"Spontaneous structural transition in phospholipid-inspired aromatic phosphopeptide nanostructures","volume":"9","author":"Pellach","year":"2015","journal-title":"ACS Nano"},{"key":"10.1016\/j.jchromb.2020.122070_b0120","series-title":"Fmoc Solid Phase Pept. Synth.","first-page":"41","article-title":"Basic procedures","author":"Chan","year":"2000"},{"key":"10.1016\/j.jchromb.2020.122070_b0125","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/0003-2697(70)90146-6","article-title":"Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides","volume":"34","author":"Kaiser","year":"1970","journal-title":"Anal Biochem."},{"key":"10.1016\/j.jchromb.2020.122070_b0130","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1021\/co1000663","article-title":"Affinity chromatography based on a combinatorial strategy for rerythropoietin purification","volume":"13","author":"Mart\u00ednez-Ceron","year":"2011","journal-title":"ACS Comb. Sci."},{"key":"10.1016\/j.jchromb.2020.122070_b0135","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.jchromb.2020.122070_b0140","doi-asserted-by":"crossref","first-page":"e26","DOI":"10.21769\/BioProtoc.26","article-title":"Silver Staining","volume":"1","author":"Aboulaich","year":"2011","journal-title":"Bio-Protocol."},{"key":"10.1016\/j.jchromb.2020.122070_b0145","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/nprot.2006.4","article-title":"Tricine\u2013SDS-PAGE","volume":"1","author":"Sch\u00e4gger","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jchromb.2020.122070_b0150","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2020.122070_b0155","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1016\/S0022-2275(20)32226-4","article-title":"A simple assay for a human serum phospholipase A2 that is associated with high-density lipoproteins","volume":"42","author":"Petrovic","year":"2001","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jchromb.2020.122070_b0160","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1002\/prot.21980","article-title":"Insights into the role of oligomeric state on the biological activities of crotoxin: Crystal structure of a tetrameric phospholipase A2 formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom","volume":"72","author":"Marchi-Salvador","year":"2008","journal-title":"Proteins Struct. Funct. Bioinforma."},{"key":"10.1016\/j.jchromb.2020.122070_b0165","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.jmb.2011.07.027","article-title":"Crystal Structure of Crotoxin Reveals Key Residues Involved in the Stability and Toxicity of This Potent Heterodimeric \u03b2-Neurotoxin","volume":"412","author":"Faure","year":"2011","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jchromb.2020.122070_b0170","first-page":"473","article-title":"Targets and Therapeutic Properties","author":"Beeton","year":"2013","journal-title":"Handb. Biol. Act. Pept."},{"key":"10.1016\/j.jchromb.2020.122070_b0175","doi-asserted-by":"crossref","first-page":"675985","DOI":"10.1155\/2014\/675985","article-title":"State of the art in the studies on crotamine, a cell penetrating peptide from South American rattlesnake","volume":"2014","author":"Kerkis","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.jchromb.2020.122070_b0180","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.bbamem.2005.06.014","article-title":"Inhibition of crotoxin binding to synaptosomes by a receptor-like protein from Crotalus durissus terrificus (the South American rattlesnake)","volume":"1717","author":"Dos Santos","year":"2005","journal-title":"Biochim. Biophys. Acta - Biomembr."},{"key":"10.1016\/j.jchromb.2020.122070_b0185","doi-asserted-by":"crossref","unstructured":"G. Faure, D. Porowinska, F. Saul, Crotoxin from Crotalus durissus terrificus and Crotoxin-Related Proteins: Structure and Function Relationship, in: 2017, pp. 3\u201320. https:\/\/doi.org\/10.1007\/978-94-007-6452-1_7.","DOI":"10.1007\/978-94-007-6452-1_7"},{"key":"10.1016\/j.jchromb.2020.122070_b0190","doi-asserted-by":"crossref","unstructured":"L. Alves de Oliveira, R. Seabra Ferreira Jr, B. Barraviera, F. Capel Tavares de Carvalho, L. Curtolo de Barros, L. Delazari dos Santos, D. Carvalho Pimenta, Crotalus durissus terrificus crotapotin naturally displays preferred positions for amino acid substitutions, (n.d.). https:\/\/doi.org\/10.1186\/s40409-017-0136-5.","DOI":"10.1186\/s40409-017-0136-5"},{"key":"10.1016\/j.jchromb.2020.122070_b0195","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0167-7306(08)60337-3","article-title":"Chapter 9 Phospholipases","volume":"20","author":"Waite","year":"1991","journal-title":"New Compr. Biochem."},{"key":"10.1016\/j.jchromb.2020.122070_b0200","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.toxicon.2013.01.006","article-title":"Individual venom profiling of Crotalus durissus terrificus specimens from a geographically limited region: Crotamine assessment and captivity evaluation on the biological activities","volume":"69","author":"Louren\u00e7o","year":"2013","journal-title":"Toxicon."}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023219315430?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023219315430?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T22:59:58Z","timestamp":1761605998000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023219315430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":40,"alternative-id":["S1570023219315430"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2020.122070","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Use of a phosphopeptide as a ligand to purify phospholipase A2 from the venom of Crotalus durisuss terrificus by affinity chromatography","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2020.122070","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"122070"}}