{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:34:01Z","timestamp":1786991641881,"version":"build-2736575974"},"reference-count":73,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["411774929"],"award-info":[{"award-number":["411774929"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FR 830\/21-1"],"award-info":[{"award-number":["FR 830\/21-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1016\/j.jchromb.2021.122853","type":"journal-article","created":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T03:44:47Z","timestamp":1625629487000},"page":"122853","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Isolation of primary human B lymphocytes from tonsils compared to blood as alternative source for ex vivo application"],"prefix":"10.1016","volume":"1179","author":[{"given":"M.","family":"Helm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"A. B. Riedl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Gollner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Gollner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"J\u00e9r\u00f4me","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Freitag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2021.122853_b0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2019.02458","article-title":"Challenges and opportunities for consistent classification of human B cell and plasma cell populations","volume":"10","author":"Sanz","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0010","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jbiotec.2010.03.001","article-title":"Immunological substance testing on human lymphatic micro-organoids in vitro","volume":"148","author":"Giese","year":"2010","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jchromb.2021.122853_b0015","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1182\/blood-2008-09-177139","article-title":"Engineering human hematopoietic stem\/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytes","volume":"113","author":"Luo","year":"2009","journal-title":"Blood"},{"key":"10.1016\/j.jchromb.2021.122853_b0020","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/978-1-62703-586-6_15","article-title":"Antigen-specific in vitro immunization: a source for human monoclonal antibodies","volume":"1060","author":"Tomimatsu","year":"2014","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jchromb.2021.122853_b0025","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/s41577-020-0365-7","article-title":"Passive antibody therapy in COVID-19","volume":"20","author":"Abraham","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0030","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.57877","article-title":"Antibodies to SARS-CoV-2 and their potential for therapeutic passive immunization","volume":"9","author":"Klasse","year":"2020","journal-title":"eLife"},{"key":"10.1016\/j.jchromb.2021.122853_b0035","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/s41577-020-00434-6","article-title":"Immunological considerations for COVID-19 vaccine strategies","volume":"20","author":"Jeyanathan","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0040","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.immuni.2020.06.013","article-title":"An integrated multi-omic single-cell atlas of human B cell identity","volume":"53","author":"Glass","year":"2020","journal-title":"Immunity"},{"key":"10.1016\/j.jchromb.2021.122853_b0045","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1084\/jem.170.6.2159","article-title":"CD19 is functionally and physically associated with surface immunoglobulin","volume":"170","author":"Pesando","year":"1989","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jchromb.2021.122853_b0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0162209","article-title":"Novel strategy for phenotypic characterization of human B lymphocytes from precursors to effector cells by flow cytometry","volume":"11","author":"Clavarino","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.jchromb.2021.122853_b0055","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1126\/science.7537388","article-title":"Generation of memory B cells and plasma cells in vitro","volume":"268","author":"Arpin","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.jchromb.2021.122853_b0060","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1600-065X.1997.tb00963.x","article-title":"Germinal center development","volume":"156","author":"Liu","year":"1997","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.jchromb.2021.122853_b0065","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1038\/342929a0","article-title":"Mechanism of antigen-driven selection in germinal centres","volume":"342","author":"Liu","year":"1989","journal-title":"Nature"},{"key":"10.1016\/j.jchromb.2021.122853_b0070","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1084\/jem.180.1.329","article-title":"Analysis of somatic mutation in five B cell subsets of human tonsil","volume":"180","author":"Pascual","year":"1994","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jchromb.2021.122853_b0075","doi-asserted-by":"crossref","first-page":"7867","DOI":"10.4049\/jimmunol.175.12.7867","article-title":"IL-21 induces differentiation of human naive and memory B cells into antibody-secreting plasma cells","volume":"175","author":"Ettinger","year":"2005","journal-title":"J. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0080","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1182\/blood.V81.10.2658.2658","article-title":"Phenotypic difference of normal plasma cells from mature myeloma cells","volume":"81","author":"Harada","year":"1993","journal-title":"Blood"},{"key":"10.1016\/j.jchromb.2021.122853_b0085","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1172\/JCI18025","article-title":"BAFF selectively enhances the survival of plasmablasts generated from human memory B cells","volume":"112","author":"Avery","year":"2003","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jchromb.2021.122853_b0090","doi-asserted-by":"crossref","first-page":"8180","DOI":"10.4049\/jimmunol.179.12.8180","article-title":"Cytokine-mediated regulation of human B cell differentiation into Ig-secreting cells: Predominant role of IL-21 produced by CXCR5+ T follicular helper cells","volume":"179","author":"Bryant","year":"2007","journal-title":"J. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0095","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1002\/btpr.2265","article-title":"Prolonged Ex vivo expansion and differentiation of na\u00efve murine CD43\u2212 B splenocytes","volume":"32","author":"Zambrano","year":"2016","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.jchromb.2021.122853_b0100","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.4049\/jimmunol.1302783","article-title":"Tissue distribution and dependence of responsiveness of human antigen-specific memory B cells","volume":"192","author":"Giesecke","year":"2014","journal-title":"J. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0105","series-title":"Preparation of lymphocytes and identification of lymphocyte subpopulations","author":"Plebanksi","year":"1999"},{"key":"10.1016\/j.jchromb.2021.122853_b0110","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.4049\/jimmunol.150.9.3671","article-title":"Human B cell proliferation and Ig secretion induced by recombinant CD40 ligand are modulated by soluble cytokines","volume":"150","author":"Armitage","year":"1993","journal-title":"J. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0115","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1084\/jem.178.5.1473","article-title":"Interleukin 10 (IL-10) upregulates functional high affinity IL-2 receptors on normal and leukemic B lymphocytes","volume":"178","author":"Fluckiger","year":"1993","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jchromb.2021.122853_b0120","doi-asserted-by":"crossref","first-page":"7.8.1-","DOI":"10.1002\/0471142735.im0708s86","article-title":"Isolation of mononuclear cells from tonsillar tissue","volume":"86","author":"Johnston","year":"2009","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0125","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/S0167-5699(98)01307-3","article-title":"Immunology of the tonsils","volume":"19","author":"Perry","year":"1998","journal-title":"Immunol. Today"},{"key":"10.1016\/j.jchromb.2021.122853_b0130","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1177\/014107689008300216","article-title":"Immunology of the tonsil: a review","volume":"83","author":"Scadding","year":"1990","journal-title":"J. R. Soc. Med."},{"key":"10.1016\/j.jchromb.2021.122853_b0135","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1046\/j.1365-2249.1999.00850.x","article-title":"Age-dependent altered proportions in subpopulations of tonsillar lymphocytes","volume":"116","author":"Bergler","year":"1999","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0140","first-page":"17","article-title":"Evolving abundance and clonal pattern of human germinal center B cells during childhood","volume":"76","author":"Valles-Ayoub","year":"1990","journal-title":"Blood"},{"key":"10.1016\/j.jchromb.2021.122853_b0145","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/0090-1229(80)90028-8","article-title":"Age-dependent changes in human peripheral blood B cells and T-cell subsets: correlation with mitogen responsiveness","volume":"15","author":"Cobleigh","year":"1980","journal-title":"Clin. Immunol. Immunopathol."},{"key":"10.1016\/j.jchromb.2021.122853_b0150","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2019.02434","article-title":"CD maps\u2014Dynamic profiling of CD1\u2013CD100 surface expression on human leukocyte and lymphocyte subsets","volume":"10","author":"Kalina","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0155","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1002\/cyto.990110203","article-title":"High gradient magnetic cell separation with MACS","volume":"11","author":"Miltenyi","year":"1990","journal-title":"Cytometry"},{"key":"10.1016\/j.jchromb.2021.122853_b0160","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0029806","article-title":"Poke weed mitogen requires Toll-like receptor ligands for proliferative activity in human and murine B lymphocytes","volume":"7","author":"Bekeredjian-Ding","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.jchromb.2021.122853_b0165","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1126\/science.285.5425.260","article-title":"BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator","volume":"285","author":"Moore","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.jchromb.2021.122853_b0170","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0022-1759(01)00466-5","article-title":"Two-step negative enrichment of CD4+ and CD8+ T cells from murine spleen via nylon wool adherence and an optimized antibody cocktail","volume":"258","author":"Gunzer","year":"2001","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jchromb.2021.122853_b0175","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0022-1759(81)90134-4","article-title":"HLA-DR typing: A comparison between nylon wool adherence and T cell rosetting in the isolation of B cells","volume":"46","author":"Wong","year":"1981","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jchromb.2021.122853_b0180","doi-asserted-by":"crossref","first-page":"13877","DOI":"10.1038\/s41598-019-50455-y","article-title":"Synergy between B cell receptor\/antigen uptake and MHCII peptide editing relies on HLA-DO tuning","volume":"9","author":"Jiang","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jchromb.2021.122853_b0185","doi-asserted-by":"crossref","first-page":"571","DOI":"10.3109\/08820137209022965","article-title":"Isolation of pure human peripheral blood T-lymphocytes using nylon wool columns","volume":"1","author":"Eisen","year":"1972","journal-title":"Immunol. Commun."},{"key":"10.1016\/j.jchromb.2021.122853_b0190","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1002\/eji.1830031011","article-title":"A rapid method for the isolation of functional thymus-derived murine lymphocytes","volume":"3","author":"Julius","year":"1973","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0195","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.4049\/jimmunol.113.4.1093","article-title":"Separation of T and B lymphocytes by nylon wool columns: Evaluation of efficacy by functional assays in vivo","volume":"113","author":"Trizio","year":"1974","journal-title":"J. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0200","first-page":"544","article-title":"Separation of murine lymphoid cells using nylon wool columns","volume":"18","author":"Handwerger","year":"1974","journal-title":"Recovery of the B cell-enriched population, Transplantation"},{"key":"10.1016\/j.jchromb.2021.122853_b0205","doi-asserted-by":"crossref","DOI":"10.1002\/cti2.1090","article-title":"Distinguishing naive- from memory-derived human B cells during acute responses","volume":"8","author":"Auladell","year":"2019","journal-title":"Clin Trans Immunol"},{"key":"10.1016\/j.jchromb.2021.122853_b0210","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1073\/pnas.58.5.2020","article-title":"Isolation and characterization of a mitogen from pokeweek (Phytolacca americana)","volume":"58","author":"Reisfeld","year":"1967","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jchromb.2021.122853_b0215","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2011.00035","article-title":"Reproducible isolation of lymph node stromal cells reveals site-dependent differences in fibroblastic reticular cells","volume":"2","author":"Fletcher","year":"2011","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0220","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0167555","article-title":"A reproducible method for isolation and in vitro culture of functional human lymphoid stromal cells from tonsils","volume":"11","author":"Bar-Ephraim","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.jchromb.2021.122853_b0225","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2012.02.012","article-title":"New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes","volume":"379","author":"Quah","year":"2012","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jchromb.2021.122853_b0230","unstructured":"I. Terr\u00e9n, A. Orrantia, J. Vitall\u00e9, O. Zenarruzabeitia, F. Borrego, Chapter Twelve - CFSE dilution to study human T and NK cell proliferation in vitro, in: L. Galluzzi, N.-P. Rudqvist (Eds.) Methods Enzymol., Academic Press2020, pp. 239-255."},{"key":"10.1016\/j.jchromb.2021.122853_b0235","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1016\/S0002-9440(10)64841-9","article-title":"Human nasopharyngeal-associated lymphoreticular tissues. Functional analysis of subepithelial and intraepithelial B and T cells from adenoids and tonsils","volume":"157","author":"Boyaka","year":"2000","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.jchromb.2021.122853_b0240","first-page":"95","article-title":"Increased proportion of B lymphocytes in human tonsils and appendices","volume":"55","author":"Alexopoulos","year":"1976","journal-title":"Acta Haematol."},{"key":"10.1016\/j.jchromb.2021.122853_b0245","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/BF00469732","article-title":"Percent distribution of T- and B-cells in tonsils of children, juveniles and adults","volume":"218","author":"Sesterhenn","year":"1977","journal-title":"Arch. Otorhinolaryngol."},{"key":"10.1016\/j.jchromb.2021.122853_b0250","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1002\/cyto.b.21589","article-title":"Changes in tonsil B cell phenotypes and EBV receptor expression in children under 5-years-old","volume":"94","author":"Wohlford","year":"2018","journal-title":"Cytometry B Clin Cytom."},{"key":"10.1016\/j.jchromb.2021.122853_b0255","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/eji.1830080202","article-title":"Role of adherent cells in immune responses to phytohemagglutinin and concanavalin A","volume":"8","author":"Arala-Chaves","year":"1978","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0260","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1084\/jem.142.5.1041","article-title":"The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor","volume":"142","author":"Stout","year":"1975","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jchromb.2021.122853_b0265","doi-asserted-by":"crossref","DOI":"10.1002\/0471142735.im0705s75","article-title":"Isolation of human B cell populations","author":"Sims","year":"2006","journal-title":"Curr. Protoc. Immunol., Chapter 7"},{"key":"10.1016\/j.jchromb.2021.122853_b0270","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2019.00588","article-title":"Lessons to learn from low-dose cyclosporin-A: A new approach for unexpected clinical applications","volume":"10","author":"Flores","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0275","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1089\/aid.2017.0208","article-title":"An optimized and validated method for isolation and characterization of lymphocytes from HIV+ human gut biopsies","volume":"33","author":"Trapecar","year":"2017","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"10.1016\/j.jchromb.2021.122853_b0280","first-page":"378","article-title":"Greatly increased autoantibody production in myasthenia gravis by thymocyte suspensions prepared with proteolytic enzymes","volume":"54","author":"Willcox","year":"1983","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0285","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0165-5876(01)00422-0","article-title":"Lymphocyte subpopulations in hypertrophied adenoid in children","volume":"59","author":"Musiatowicz","year":"2001","journal-title":"Int. J. Pediatr. Otorhinolaryngol."},{"key":"10.1016\/j.jchromb.2021.122853_b0290","doi-asserted-by":"crossref","first-page":"4883","DOI":"10.1016\/j.febslet.2010.08.022","article-title":"Receptors and signaling mechanisms for B-lymphocyte activation, proliferation and differentiation \u2013 Insights from both in vivo and in vitro approaches","volume":"584","author":"Maddaly","year":"2010","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jchromb.2021.122853_b0295","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.3324\/haematol.2019.243543","article-title":"The regulation and function of CD20: an \u201cenigma\u201d of B-cell biology and targeted therapy","volume":"105","author":"Pavlasova","year":"2020","journal-title":"Haematologica"},{"key":"10.1016\/j.jchromb.2021.122853_b0300","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1016\/j.celrep.2020.02.022","article-title":"The interplay between CD27dull and CD27bright B cells ensures the flexibility, stability, and resilience of human B cell memory","volume":"30","author":"Grimsholm","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.jchromb.2021.122853_b0305","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s10238-015-0338-5","article-title":"Age-related differences in human palatine tonsillar B cell subsets and immunoglobulin isotypes","volume":"16","author":"Lee","year":"2016","journal-title":"Clin. Exp. Med."},{"key":"10.1016\/j.jchromb.2021.122853_b0310","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1182\/blood.V99.6.2154","article-title":"The heterogeneity shown by human plasma cells from tonsil, blood, and bone marrow reveals graded stages of increasing maturity, but local profiles of adhesion molecule expression","volume":"99","author":"Medina","year":"2002","journal-title":"Blood"},{"key":"10.1016\/j.jchromb.2021.122853_b0315","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.jim.2003.12.015","article-title":"Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system","volume":"286","author":"Crotty","year":"2004","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jchromb.2021.122853_b0320","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1038\/icb.2014.59","article-title":"Human memory B cells isolated from blood and tonsils are functionally distinctive","volume":"92","author":"P\u00e9rez","year":"2014","journal-title":"Immunol. Cell Biol."},{"key":"10.1016\/j.jchromb.2021.122853_b0325","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0213832","article-title":"Isolation of B-cells using Miltenyi MACS bead isolation kits","volume":"14","author":"Moore","year":"2019","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.jchromb.2021.122853_b0330","first-page":"455","article-title":"Characterization of the B lymphocyte response to pokeweed mitogen","volume":"12","author":"Schreck","year":"1982","journal-title":"Ann. Clin. Lab. Sci."},{"key":"10.1016\/j.jchromb.2021.122853_b0335","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.cell.2019.03.016","article-title":"B cell responses: Cell interaction dynamics and decisions","volume":"177","author":"Cyster","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.jchromb.2021.122853_b0340","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0065-2776(08)60876-7","article-title":"Human B lymphocytes: Phenotype, proliferation, and differentiation","author":"Banchereau","year":"1992","journal-title":"Adv. Immunol., Academic Press"},{"key":"10.1016\/j.jchromb.2021.122853_b0345","series-title":"Clinical Immunology (Fifth Edition)","first-page":"1253","article-title":"Assessment of functional immune responses in lymphocytes","author":"Abraham","year":"2019"},{"key":"10.1016\/j.jchromb.2021.122853_b0350","series-title":"Encyclopedia of Immunology (Second Edition)","first-page":"1978","article-title":"Pokeweed Mitogen (PWM)","author":"Horii","year":"1998"},{"key":"10.1016\/j.jchromb.2021.122853_b0355","doi-asserted-by":"crossref","first-page":"5886","DOI":"10.4049\/jimmunol.179.9.5886","article-title":"Essential Role of IL-21 in B Cell Activation, Expansion, and Plasma Cell Generation during CD4+ T Cell-B Cell Collaboration","volume":"179","author":"Kuchen","year":"2007","journal-title":"J Immunol."},{"key":"10.1016\/j.jchromb.2021.122853_b0360","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1056\/NEJM198008143030704","article-title":"Current concepts in immunology: Regulation of the immune response-inducer and suppressor T-lymphocyte subsets in human beings","volume":"303","author":"Reinherz","year":"1980","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jchromb.2021.122853_b0365","doi-asserted-by":"crossref","first-page":"261","DOI":"10.4049\/jimmunol.170.1.261","article-title":"A division-linked mechanism for the rapid generation of Ig-secreting cells from human memory B cells","volume":"170","author":"Tangye","year":"2003","journal-title":"J Immunol."}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023221003342?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023221003342?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T21:20:01Z","timestamp":1778620801000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023221003342"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8]]},"references-count":73,"alternative-id":["S1570023221003342"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2021.122853","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2021,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isolation of primary human B lymphocytes from tonsils compared to blood as alternative source for ex vivo application","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2021.122853","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"122853"}}