{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T21:39:33Z","timestamp":1793914773959,"version":"4.8.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["10631"],"award-info":[{"award-number":["10631"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2023,8]]},"DOI":"10.1016\/j.jchromb.2023.123834","type":"journal-article","created":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T04:26:15Z","timestamp":1689567975000},"page":"123834","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"special_numbering":"C","title":["Simultaneous estimation of five biomarkers of neuroprotective herb Ashwagandha NMITLI-118R AF1 in rat plasma and brain using LC-ESI-MS\/MS: Application to its pharmacokinetic and stability studies"],"prefix":"10.1016","volume":"1228","author":[{"given":"Shailesh D.","family":"Dadge","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neerja","family":"Tiwari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athar","family":"Husain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh","family":"Verma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richa","family":"Garg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srikanta K.","family":"Rath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karuna","family":"Shanker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7703-9307","authenticated-orcid":false,"given":"Jiaur R.","family":"Gayen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2023.123834_b0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2021.113819","article-title":"Elucidation of plasma protein binding, blood partitioning, permeability, CYP phenotyping and CYP inhibition studies of Withanone using validated UPLC method: An active constituent of neuroprotective herb Ashwagandha","volume":"270","author":"Singh","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jchromb.2023.123834_b0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2020.113766","article-title":"Investigation of CYP2B6, 3A4 and \u03b2-esterase interactions of Withania somnifera (L.) dunal in human liver microsomes and HepG2 cells","volume":"270","author":"Kumar","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jchromb.2023.123834_b0015","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.phytol.2021.12.007","article-title":"In silico evaluation of pharmacokinetics and acute toxicity of withanolides in Ashawagandha","volume":"47","author":"Liu","year":"2022","journal-title":"Phytochem. Lett."},{"key":"10.1016\/j.jchromb.2023.123834_b0020","doi-asserted-by":"crossref","first-page":"3824","DOI":"10.1016\/0031-9422(91)80125-K","article-title":"Two withanolides fromWithania somnifera","volume":"30","author":"Jamal","year":"1991","journal-title":"Phytochemistry"},{"key":"10.1016\/j.jchromb.2023.123834_b0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2022.154137","article-title":"Withania somnifera-A magic plant targeting multiple pathways in cancer related inflammation","author":"Kumar","year":"2022","journal-title":"Phytomedicine"},{"key":"10.1016\/j.jchromb.2023.123834_b0030","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/ddr.21463","article-title":"Determination of permeability, plasma protein binding, blood partitioning, pharmacokinetics and tissue distribution of Withanolide A in rats: A neuroprotective steroidal lactone","volume":"79","author":"Singh","year":"2018","journal-title":"Drug Dev. Res."},{"key":"10.1016\/j.jchromb.2023.123834_b0035","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.3390\/jcm9082544","article-title":"Telomeres, DNA damage and ageing: potential leads from ayurvedic rasayana (anti-ageing) drugs","author":"Sharma","year":"2020","journal-title":"Journal of Clinical Medicine,"},{"key":"10.1016\/j.jchromb.2023.123834_b0040","doi-asserted-by":"crossref","first-page":"e27265","DOI":"10.1371\/journal.pone.0027265","article-title":"Protective role of Ashwagandha leaf extract and its component withanone on scopolamine-induced changes in the brain and brain-derived cells","volume":"6","author":"Konar","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.jchromb.2023.123834_b0045","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s00299-006-0260-0","article-title":"Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera","volume":"26","author":"Bandyopadhyay","year":"2007","journal-title":"Plant Cell Rep."},{"key":"10.1016\/j.jchromb.2023.123834_b0050","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0021-9673(01)94423-1","article-title":"Semi-quantitative reversed-phase high-performance liquid chromatographic analysis of the ecotypes of Withania somnifera chemotype III","volume":"389","author":"Bessalle","year":"1987","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2023.123834_b0055","doi-asserted-by":"crossref","first-page":"2433","DOI":"10.1002\/jssc.200600147","article-title":"Advantages of ultra performance liquid chromatography over high\u2010performance liquid chromatography: Comparison of different analytical approaches during analysis of diclofenac gel","volume":"29","author":"Nov\u00e1kov\u00e1","year":"2006","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jchromb.2023.123834_b0060","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.3390\/molecules27051476","article-title":"Pharmacokinetic study of withanosides and withanolides from Withania somnifera using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS\/MS)","volume":"27","author":"Modi","year":"2022","journal-title":"molecules"},{"key":"10.1016\/j.jchromb.2023.123834_b0065","doi-asserted-by":"crossref","first-page":"e4573","DOI":"10.1002\/bmc.4573","article-title":"Studies on oral bioavailability and first-pass metabolism of withaferin A in rats using LC\u2013MS\/MS and Q-TRAP","volume":"33","author":"Dai","year":"2019","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.jchromb.2023.123834_b0070","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1002\/ijc.25938","article-title":"Withaferin A inhibits breast cancer invasion and metastasis at sub-cytotoxic doses by inducing vimentin disassembly and serine 56 phosphorylation","volume":"129","author":"Thaiparambil","year":"2011","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.jchromb.2023.123834_b0075","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.taap.2015.09.014","article-title":"Thiol dependent NF-\u03baB suppression and inhibition of T-cell mediated adaptive immune responses by a naturally occurring steroidal lactone Withaferin A","volume":"289","author":"Gambhir","year":"2015","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.jchromb.2023.123834_b0080","doi-asserted-by":"crossref","first-page":"26","DOI":"10.18203\/2319-2003.ijbcp20214831","article-title":"A comparative pharmacokinetics study of Ashwagandha (Withania somnifera) Root Extract sustained-release capsules: an open-label, randomized, two treatment, two-sequence, two period, single-dose crossover clinical study","volume":"11","author":"Alluri","year":"2022","journal-title":"International Journal of Basic and Clinical Pharmacology"},{"key":"10.1016\/j.jchromb.2023.123834_b0085","first-page":"461","article-title":"Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha)","author":"Sangwan","year":"2004","journal-title":"Curr. Sci."},{"key":"10.1016\/j.jchromb.2023.123834_b0090","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jchromb.2016.07.042","article-title":"Pharmacokinetics and bioavailability assessment of Miltefosine in rats using high performance liquid chromatography tandem mass spectrometry","volume":"1031","author":"Valicherla","year":"2016","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jchromb.2023.123834_b0095","article-title":"Development and validation of LC-MS\/MS method for quantification of novel PP2A\u2013\u03b2-catenin signalling inhibitor, S011\u20132111 in mice plasma: Application to its preclinical pharmacokinetic studies","volume":"1130","author":"Riyazuddin","year":"2019","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jchromb.2023.123834_b0100","doi-asserted-by":"crossref","first-page":"2198","DOI":"10.1016\/j.jchromb.2009.01.003","article-title":"Validation of bioanalytical LC\u2013MS\/MS assays: evaluation of matrix effects","volume":"877","author":"Van Eeckhaut","year":"2009","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jchromb.2023.123834_b0105","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1124\/dmd.111.044446","article-title":"disposition, Metabolism, pharmacokinetics, tissue distribution, and stability studies of the prodrug analog of an anti-hepatitis B virus dinucleoside phosphorothioate","volume":"40","author":"Coughlin","year":"2012","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.jchromb.2023.123834_b0110","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jpba.2013.03.001","article-title":"B. Analysis, Determination of withaferin A and withanolide A in mice plasma using high-performance liquid chromatography-tandem mass spectrometry: application to pharmacokinetics after oral administration of Withania somnifera aqueous extract","volume":"80","author":"Patil","year":"2013","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2023.123834_b0115","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jep.2011.01.002","article-title":"LC\u2013MS\/MS determination and pharmacokinetic study of five flavone components after solvent extraction\/acid hydrolysis in rat plasma after oral administration of Verbena officinalis L. extract","volume":"135","author":"Duan","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jchromb.2023.123834_b0120","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jchromb.2018.01.035","article-title":"LC-MS\/MS method for the simultaneous quantification of luteolin, wedelolactone and apigenin in mice plasma using hansen solubility parameters for liquid-liquid extraction: Application to pharmacokinetics of Eclipta alba chloroform fraction","volume":"1081","author":"Cheruvu","year":"2018","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jchromb.2023.123834_b0125","doi-asserted-by":"crossref","first-page":"530","DOI":"10.3109\/00498254.2014.994053","article-title":"Pharmacokinetics and tissue distribution study of novel potent antiplatelet agent S007\u2013867 in mice using HPLC-MS\/MS","volume":"45","author":"Chandasana","year":"2015","journal-title":"Xenobiotica"},{"key":"10.1016\/j.jchromb.2023.123834_b0130","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.exger.2016.03.004","article-title":"Withanolide A offers neuroprotection, ameliorates stress resistance and prolongs the life expectancy of Caenorhabditis elegans","volume":"78","author":"Akhoon","year":"2016","journal-title":"Exp. Gerontol."},{"key":"10.1016\/j.jchromb.2023.123834_b0135","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jpba.2018.06.047","article-title":"In-vitro metabolism, CYP profiling and metabolite identification of E-and Z-guggulsterone, a potent hypolipidmic agent","volume":"160","author":"Chhonker","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2023.123834_b0140","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.ijpharm.2005.03.022","article-title":"Development and application of high throughput plasma stability assay for drug discovery","volume":"297","author":"Di","year":"2005","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.jchromb.2023.123834_b0145","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/S0091-3057(03)00110-2","article-title":"Behavior, Adaptogenic activity of Withania somnifera: an experimental study using a rat model of chronic stress","volume":"75","author":"Bhattacharya","year":"2003","journal-title":"Pharmacology Biochemistry and Behaviour"},{"key":"10.1016\/j.jchromb.2023.123834_b0150","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0378-8741(99)00206-8","article-title":"Immunomodulatory activity of Withania somnifera","volume":"71","author":"Davis","year":"2000","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jchromb.2023.123834_b0155","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1080\/09712119.1993.9705964","article-title":"Anti-inflammatory activity of Withania somnifera: Possible mode of action","volume":"3","author":"Sahni","year":"1993","journal-title":"J. Appl. Anim. Res."},{"key":"10.1016\/j.jchromb.2023.123834_b0160","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.phytochem.2015.02.029","article-title":"Hypoglycemic activity of withanolides and elicitated Withania somnifera","volume":"116","author":"Gorelick","year":"2015","journal-title":"Phytochemistry"},{"key":"10.1016\/j.jchromb.2023.123834_b0165","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.3109\/13880209.2016.1157193","article-title":"Hepatoprotective effect of withanolide-rich fraction in acetaminophen-intoxicated rat: decisive role of TNF-\u03b1, IL-1\u03b2, COX-II and iNOS","volume":"54","author":"Devkar","year":"2016","journal-title":"Pharm. Biol."},{"key":"10.1016\/j.jchromb.2023.123834_b0170","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/BF02864860","article-title":"R.J.I.J.o.C.B.A biologically active constituent of withania somnifera (ashwagandha) with antistress activity","volume":"16","author":"Kaur","year":"2001","journal-title":"Indian J. Clin. Biochem."},{"key":"10.1016\/j.jchromb.2023.123834_b0175","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1191\/0960327105ht509oa","article-title":"e. toxicology, Neuroprotective effects of Withania somnifera on 6-hydroxydopamine induced Parkinsonism in rats","volume":"24","author":"Ahmad","year":"2005","journal-title":"Hum. Exp. Toxicol."},{"key":"10.1016\/j.jchromb.2023.123834_b0180","article-title":"Efficacy and safety of Ashwagandha (Withania somnifera) root extract in insomnia and anxiety: a double-blind, randomized, placebo-controlled study","volume":"11","author":"Langade","year":"2019","journal-title":"Cureus"},{"key":"10.1016\/j.jchromb.2023.123834_b0185","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/4076430","article-title":"Effects of Withania somnifera on reproductive system: a systematic review of the available evidence","author":"Nasimi Doost Azgomi","year":"2018","journal-title":"Biomed Res. Int."},{"key":"10.1016\/j.jchromb.2023.123834_b0190","doi-asserted-by":"crossref","first-page":"255","DOI":"10.4103\/0253-7176.106022","article-title":"A prospective, randomized double-blind, placebo-controlled study of safety and efficacy of a high-concentration full-spectrum extract of ashwagandha root in reducing stress and anxiety in adults","volume":"34","author":"Chandrasekhar","year":"2012","journal-title":"Indian J. Psychol. Med."},{"key":"10.1016\/j.jchromb.2023.123834_b0195","doi-asserted-by":"crossref","first-page":"241","DOI":"10.4103\/0975-9476.146566","article-title":"Subtle changes in thyroid indices during a placebo-controlled study of an extract of Withania somnifera in persons with bipolar disorder","volume":"5","author":"Gannon","year":"2014","journal-title":"Journal of Ayurveda and Integrative Medicine."},{"key":"10.1016\/j.jchromb.2023.123834_b0200","doi-asserted-by":"crossref","first-page":"111","DOI":"10.4103\/0975-9476.100168","article-title":"Exploratory study to evaluate tolerability, safety, and activity of Ashwagandha (Withania somnifera) in healthy volunteers","volume":"3","author":"Raut","year":"2012","journal-title":"Journal of Ayurveda and Integrative Medicine."}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023223002441?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023223002441?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T23:11:51Z","timestamp":1761606711000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023223002441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":40,"alternative-id":["S1570023223002441"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2023.123834","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simultaneous estimation of five biomarkers of neuroprotective herb Ashwagandha NMITLI-118R AF1 in rat plasma and brain using LC-ESI-MS\/MS: Application to its pharmacokinetic and stability studies","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2023.123834","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"123834"}}