{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T17:02:00Z","timestamp":1789232520547,"version":"build-2803163510"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013804","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.jchromb.2024.124325","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T11:58:50Z","timestamp":1726833530000},"page":"124325","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Preparative separation of ten flavonoids from Scutellaria baicalensis Georgi roots using two-dimensional countercurrent chromatography with an online-storage, dilution, and mixing interface"],"prefix":"10.1016","volume":"1247","author":[{"given":"Lanjie","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxing","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1109-9531","authenticated-orcid":false,"given":"Zhi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2024.124325_b0005","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1126\/science.167.3916.281","article-title":"R L., Countercurrent chromatography: liquid-liquid partition chromatography without solid support","volume":"167","author":"Ito","year":"1970","journal-title":"Sci."},{"key":"10.1016\/j.jchromb.2024.124325_b0010","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.trac.2015.08.006","article-title":"An overview of recent progress in elution mode of counter current chromatography","volume":"77","author":"Huang","year":"2016","journal-title":"Trac-Trend Anal. Chem."},{"issue":"2","key":"10.1016\/j.jchromb.2024.124325_b0015","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.chroma.2007.01.105","article-title":"Sample capacity in preparative high-speed counter-current chromatography","volume":"1146","author":"Zhao","year":"2007","journal-title":"J. Chromatogr. A"},{"issue":"7","key":"10.1016\/j.jchromb.2024.124325_b0020","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1021\/np501065h","article-title":"Countercurrent separation of natural products: An update","volume":"78","author":"Friesen","year":"2015","journal-title":"J. Nat. Prod."},{"issue":"17","key":"10.1016\/j.jchromb.2024.124325_b0025","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.1080\/10826070500225234","article-title":"GUESS - a generally useful estimate of solvent systems in CCC","volume":"28","author":"Friesen","year":"2005","journal-title":"J. Liq. Chromatogr. Relat. Technol."},{"key":"10.1016\/j.jchromb.2024.124325_b0030","first-page":"186","article-title":"Comprehensive multidimensional liquid chromatography for advancing environmental and natural products research, Trac-Trend","volume":"116","author":"Brandao","year":"2019","journal-title":"Anal. Chem."},{"issue":"8","key":"10.1016\/j.jchromb.2024.124325_b0035","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1002\/pca.3168","article-title":"Two-dimensional chromatography in screening of bioactive components from natural products","volume":"33","author":"Xiang","year":"2022","journal-title":"Phytochem. Analysis"},{"issue":"1\u20132","key":"10.1016\/j.jchromb.2024.124325_b0040","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/S0021-9673(97)01273-9","article-title":"Multidimensional counter-current chromatographic system and its application","volume":"803","author":"Yang","year":"1998","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2024.124325_b0045","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.chroma.2013.10.095","article-title":"Comprehensive multi-channel multi-dimensional counter-current chromatography for separation of tanshinones from Salvia miltiorrhiza Bunge","volume":"1323","author":"Meng","year":"2014","journal-title":"J. Chromatogr. A"},{"issue":"20","key":"10.1016\/j.jchromb.2024.124325_b0050","doi-asserted-by":"crossref","first-page":"10172","DOI":"10.1021\/acs.analchem.5b02859","article-title":"Heart-cut two-dimensional countercurrent chromatography with a single instrument","volume":"87","author":"Englert","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jchromb.2024.124325_b0055","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.chroma.2019.03.012","article-title":"Improving the resolution of overlapping peaks by heartcut two-dimensional countercurrent chromatography with the same solvent system in both dimensions","volume":"1596","author":"Muller","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2024.124325_b0060","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.indcrop.2018.09.034","article-title":"An efficient method for the preparative separation and isolation of ginkgolic acids from the sarcotesta of Ginkgo biloba L by pH-zone-refining countercurrent chromatography coupled with inner-recycling mode","volume":"126","author":"Wang","year":"2018","journal-title":"Ind. Crop Prod."},{"issue":"7","key":"10.1016\/j.jchromb.2024.124325_b0065","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1007\/s00217-021-03751-9","article-title":"Polyphenols separated from Enteromorpha clathrata by one-dimensional coupled with inner-recycling high-speed counter-current chromatography and their antioxidant activities","volume":"247","author":"Wang","year":"2021","journal-title":"Eur. Food Res. Technol."},{"issue":"2","key":"10.1016\/j.jchromb.2024.124325_b0070","doi-asserted-by":"crossref","DOI":"10.3390\/molecules23020505","article-title":"Separation of seven polyphenols from the rhizome of smilax glabra by offline two dimension recycling HSCCC with extrusion mode","volume":"23","author":"Guo","year":"2018","journal-title":"Molecules"},{"issue":"3","key":"10.1016\/j.jchromb.2024.124325_b0075","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.seppur.2011.01.003","article-title":"Two-step preparation of ginsenoside-Re, Rb-1, Rc and Rb-2 from the root of Panax ginseng by high-performance counter-current chromatography","volume":"77","author":"Cheng","year":"2011","journal-title":"Sep. Purif. Technol."},{"issue":"46","key":"10.1016\/j.jchromb.2024.124325_b0080","doi-asserted-by":"crossref","first-page":"15840","DOI":"10.1021\/acs.iecr.8b04167","article-title":"Preparative separation of ginkgolic acids from the sarcotesta of Ginkgo biloba L. by beta-cyclodextrin clathration coupled with pH-zone-refining and recycling countercurrent chromatography","volume":"57","author":"Yan","year":"2018","journal-title":"Ind. Eng. Chem. Res."},{"issue":"1\u20132","key":"10.1016\/j.jchromb.2024.124325_b0085","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.chroma.2007.02.099","article-title":"Two-dimensional counter-current chromatography for the preparative separation of prenylflavonoids from Artocarpus altilis","volume":"1151","author":"Lu","year":"2007","journal-title":"J. Chromatogr. A"},{"issue":"7","key":"10.1016\/j.jchromb.2024.124325_b0090","doi-asserted-by":"crossref","first-page":"3081","DOI":"10.1021\/ac100121j","article-title":"Integrated countercurrent extraction of natural products: a combination of liquid and solid supports","volume":"82","author":"Lu","year":"2010","journal-title":"Anal. Chem."},{"issue":"36","key":"10.1016\/j.jchromb.2024.124325_b0095","doi-asserted-by":"crossref","first-page":"6085","DOI":"10.1016\/j.chroma.2010.12.084","article-title":"Two-dimensional counter-current chromatography: 1st traditional counter-current chromatography, 2nd acid-base elution counter-current chromatography","volume":"1218","author":"Hu","year":"2011","journal-title":"J. Chromatogr. A"},{"issue":"3","key":"10.1016\/j.jchromb.2024.124325_b0100","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.ijantimicag.2009.03.007","article-title":"In vitro antifungal activity of the aqueous extract of Scutellaria baicalensis Georgi root against Candida albicans","volume":"34","author":"SingWong","year":"2009","journal-title":"Int. J. Antimicrob. Ag."},{"key":"10.1016\/j.jchromb.2024.124325_b0105","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2021.697704","article-title":"Exploring the potential molecular mechanism of Scutellaria baicalensis Georgi in the treatment of gastric cancer based on network pharmacological analysis and molecular docking technology","volume":"12","author":"Tu","year":"2021","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.jchromb.2024.124325_b0110","article-title":"Research progress of active ingredients of Scutellaria baicalensis in the treatment of type 2 diabetes and its complications","volume":"148","author":"Wang","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.jchromb.2024.124325_b0115","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.ejmech.2017.03.004","article-title":"Therapeutic potentials of baicalin and its aglycone, baicalein against inflammatory disorders","volume":"131","author":"Dinda","year":"2017","journal-title":"Eur. J. Med. Chem."},{"issue":"3","key":"10.1016\/j.jchromb.2024.124325_b0120","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/S0304-4165(99)00152-X","article-title":"Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi","volume":"1472","author":"Gao","year":"1999","journal-title":"BBA-Gen. Subjects"},{"issue":"18","key":"10.1016\/j.jchromb.2024.124325_b0125","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1007\/s11434-016-1136-5","article-title":"Scutellaria baicalensis, the golden herb from the garden of Chinese medicinal plants","volume":"61","author":"Zhao","year":"2016","journal-title":"Sci. Bull."},{"issue":"1\u20132","key":"10.1016\/j.jchromb.2024.124325_b0130","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.chroma.2003.08.012","article-title":"Application of high-speed counter-current chromatography to the preparative separation and purification of baicalin from the chinese medicinal plant Scutellaria baicalensis","volume":"1017","author":"Lu","year":"2003","journal-title":"J. Chromatogr. A"},{"issue":"6","key":"10.1016\/j.jchromb.2024.124325_b0135","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1002\/jssc.202100918","article-title":"An efficient strategy based on two-stage chromatography and in vitro evaluation for rapid screening and isolation of acetylcholinesterase inhibitors from Scutellaria baicalensis Georgi","volume":"45","author":"Hou","year":"2022","journal-title":"J. Sep. Sci."},{"issue":"16","key":"10.1016\/j.jchromb.2024.124325_b0140","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1080\/10826076.2013.830269","article-title":"Preparative isolation of baicalin and wogonoside from scutellariae radix by high speed counter-current chromatography by using ionic liquids as the modifier of two-phase solvent system","volume":"37","author":"Wang","year":"2014","journal-title":"J. Liq. Chromatogr. Relat. Technol."},{"issue":"1\u20132","key":"10.1016\/j.jchromb.2024.124325_b0145","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.chroma.2005.01.054","article-title":"Separation and purification of baicalin and wogonoside from the Chinese medicinal plant Scutellaria baicalensis Georgi by high-speed counter-current chromatography","volume":"1066","author":"Wu","year":"2005","journal-title":"J. Chromatogr. A"},{"issue":"1\u20132","key":"10.1016\/j.jchromb.2024.124325_b0150","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.chroma.2005.03.088","article-title":"Isolation and puritication of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography","volume":"1074","author":"Li","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2024.124325_b0155","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aca.2018.01.014","article-title":"Bioactivity-guided cut countercurrent chromatography for isolation of lysine-specific demethylase 1 inhibitors from Scutellaria baicalensis Georgi","volume":"1016","author":"Han","year":"2018","journal-title":"Anal. Chim. Acta"},{"issue":"2","key":"10.1016\/j.jchromb.2024.124325_b0160","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.chroma.2004.12.044","article-title":"Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography","volume":"1065","author":"Ito","year":"2005","journal-title":"J. Chromatogr. A"},{"issue":"6","key":"10.1016\/j.jchromb.2024.124325_b0165","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1002\/jssc.201201038","article-title":"Novel linear and step-gradient counter-current chromatography for bio-guided isolation and purification of cytotoxic podophyllotoxins from Dysosma versipellis (Hance)","volume":"36","author":"Yang","year":"2013","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jchromb.2024.124325_b0170","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.chroma.2019.06.055","article-title":"Methanol linear gradient counter-current chromatography for the separation of natural products: Sinopodophyllum hexandrum as samples","volume":"1603","author":"Yang","year":"2019","journal-title":"J. Chromatogr. A"},{"issue":"2","key":"10.1016\/j.jchromb.2024.124325_b0175","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s00216-005-0016-7","article-title":"Alkane effect in the Arizona liquid systems used in countercurrent chromatography","volume":"383","author":"Berthod","year":"2005","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jchromb.2024.124325_b0180","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jchromb.2015.06.011","article-title":"Online polar two phase countercurrent chromatography \u00d7 high performance liquid chromatography for preparative isolation of polar polyphenols from tea extract in a single step","volume":"997","author":"Chen","year":"2015","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jchromb.2024.124325_b0185","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.chroma.2014.01.029","article-title":"Two-dimensional countercurrent chromatography \u00d7 high performance liquid chromatography for preparative isolation of toad venom","volume":"1331","author":"Qiu","year":"2014","journal-title":"J. Chromatogr. A"},{"issue":"36","key":"10.1016\/j.jchromb.2024.124325_b0190","doi-asserted-by":"crossref","first-page":"6122","DOI":"10.1016\/j.chroma.2011.06.073","article-title":"Comparison of preparative reversed phase liquid chromatography and countercurrent chromatography for the kilogram scale purification of crude spinetoram insecticide","volume":"1218","author":"DeAmicis","year":"2011","journal-title":"J. Chromatogr. A"},{"issue":"1","key":"10.1016\/j.jchromb.2024.124325_b0195","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9673(96)00565-1","article-title":"pH-zone-refining countercurrent chromatography","volume":"753","author":"Ito","year":"1996","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2024.124325_b0200","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.chroma.2016.02.079","article-title":"A targeted strategy to analyze untargeted mass spectral data: Rapid chemical profiling of Scutellaria baicalensis using ultra-high performance liquid chromatography coupled with hybrid quadrupole orbitrap mass spectrometry and key ion filtering","volume":"1441","author":"Qiao","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2024.124325_b0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2022.154076","article-title":"Two hydroxyflavanones isolated from Scutellaria baicalensis roots prevent colitis-associated colon cancer in C57BL\/6 J mice by inhibiting programmed cell death-1, interleukin 10, and thymocyte selection-associated high mobility group box proteins TOX\/TOX2","volume":"100","author":"Kimura","year":"2022","journal-title":"Phytomedicine"},{"key":"10.1016\/j.jchromb.2024.124325_b0210","unstructured":"H. B.H.;, L. Y.L., Studies on the structures of new flavonoids from the root of Scutellaria amoena, Acta Pharm. Sin. 24(3) (1989) 200-206. Doi: 10.16438\/j.0513-4870.1989.03.008."},{"issue":"16","key":"10.1016\/j.jchromb.2024.124325_b0215","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1080\/14786410701823967","article-title":"Preparation of high-purity baicalein from Scutellaria baicalensis Georgi","volume":"22","author":"Jiang","year":"2008","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.jchromb.2024.124325_b0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2021.112326","article-title":"Immunosuppressant flavonoids from Scutellaria baicalensis","volume":"144","author":"Syafni","year":"2021","journal-title":"Biomed. Pharmacother."}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023224003349?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023224003349?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T18:22:13Z","timestamp":1737310933000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023224003349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":44,"alternative-id":["S1570023224003349"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2024.124325","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preparative separation of ten flavonoids from Scutellaria baicalensis Georgi roots using two-dimensional countercurrent chromatography with an online-storage, dilution, and mixing interface","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2024.124325","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"124325"}}