{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:24:06Z","timestamp":1791429846229,"version":"4.3.3"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2025,4]]},"DOI":"10.1016\/j.jchromb.2025.124541","type":"journal-article","created":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T12:13:51Z","timestamp":1740658431000},"page":"124541","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Profiling neuroactive steroids in pregnancy. A non-derivatised liquid chromatography tandem mass spectrometry method for the quantitation of allopregnanolone and four related isomers in maternal serum."],"prefix":"10.1016","volume":"1256","author":[{"given":"Edward","family":"Hinchliffe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Heazell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2025.124541_bb0005","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jsbmb.2013.03.008","article-title":"Steroid profiling in pregnancy: A focus on the human fetus","volume":"139","author":"Hill","year":"2014","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0074-7742(01)46057-0","article-title":"Neurosteroids: Beginning of a story","volume":"46","author":"Baulieu","year":"2001","journal-title":"Int. Rev. Neurobiol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0015","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1126\/science.2422758","article-title":"Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor","volume":"232","author":"Majewska","year":"1986","journal-title":"Science"},{"key":"10.1016\/j.jchromb.2025.124541_bb0020","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jsbmb.2015.09.012","article-title":"Loss of neurosteroid-mediated protection following stress during fetal life","volume":"160","author":"Hirst","year":"2016","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0025","first-page":"301","article-title":"Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on fetal guinea pig brain development","volume":"208","author":"Kelleher","year":"2011","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0030","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0304-3940(01)01861-4","article-title":"Effect of finasteride on behavioural arousal and somatosensory evoked potentials in fetal sheep","volume":"306","author":"Nicol","year":"2001","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.jchromb.2025.124541_bb0035","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1159\/000097990","article-title":"Stress in pregnancy activates neurosteroid production in the fetal brain","volume":"84","author":"Hirst","year":"2006","journal-title":"Neuroendocrinology"},{"key":"10.1016\/j.jchromb.2025.124541_bb0040","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1113\/jphysiol.2004.069336","article-title":"Increased allopregnanolone levels in the fetal sheep brain following umbilical cord occlusion","volume":"560","author":"Nguyen","year":"2004","journal-title":"J. Physiol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0045","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1016\/j.neuroscience.2007.03.023","article-title":"Inhibition of neurosteroid synthesis increases asphyxia-induced brain injury in the late gestation fetal sheep","volume":"146","author":"Yawno","year":"2007","journal-title":"Neuroscience"},{"key":"10.1016\/j.jchromb.2025.124541_bb0050","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1203\/01.PDR.0000088066.47755.36","article-title":"Changes in 5\u03b1-pregnane steroids and neurosteroidogenic enzyme expression in fetal sheep with umbilicoplacental embolization","volume":"54","author":"Nguyen","year":"2003","journal-title":"Pediatr. Res."},{"key":"10.1016\/j.jchromb.2025.124541_bb0055","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0091-3057(95)02133-7","article-title":"Stress-induced increase in brain neuroactive steroids: Antagonism by abercarnil","volume":"54","author":"Barbaccia","year":"1996","journal-title":"Pharmacol. Biochem. Behav."},{"key":"10.1016\/j.jchromb.2025.124541_bb0060","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1111\/j.1365-2826.2009.01949.x","article-title":"The effect of bethamethasone treatment on neuroactive steroid synthesis in a fetal guinea pig model of growth restriction","volume":"22","author":"McKendry","year":"2010","journal-title":"J. Neuroendocrinol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0065","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1210\/er.2010-0013","article-title":"The molecular biology, biochemistry and physiology of human steroidogenesis and its disorders","volume":"32","author":"Miller","year":"2011","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.jchromb.2025.124541_bb0070","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.pneurobio.2013.08.005","article-title":"Allopregnanolone in the brain: Protecting pregnancy and birth outcomes","volume":"1134","author":"Brunton","year":"2014","journal-title":"Prog. Neurobiol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0075","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.ajog.2010.08.009","article-title":"The great obstetrical syndromes are associated with disorders of deep placentation","volume":"204","author":"Brosens","year":"2011","journal-title":"Am. J. Obstet. Gynecol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0080","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.steroids.2016.11.007","article-title":"A sensitive and accurate LC-MS\/MS assay with the derivatization of 1-amino-4-mthylepiperazine applied to serum allopregnanolone, pregnenolone and androsterone in pre- and postmenopausal women","volume":"118","author":"Ke","year":"2017","journal-title":"Steroids"},{"key":"10.1016\/j.jchromb.2025.124541_bb0085","doi-asserted-by":"crossref","first-page":"527","DOI":"10.4155\/bio-2016-0262","article-title":"LC-MS\/MS simultaneous analysis of allopregnanolone, epipregnanolone, pregnanolone, DHEA and DHEAS in human plasma","volume":"9","author":"Lionetto","year":"2017","journal-title":"Bioanalysis"},{"key":"10.1016\/j.jchromb.2025.124541_bb0090","doi-asserted-by":"crossref","first-page":"5247","DOI":"10.1007\/s00216-021-03523-0","article-title":"Development and validation of an LC-MS\/MS assay for the quantification of allopregnanolone and its progesterone-derived isomers, precursors, and cortisol\/cortisone in pregnancy","volume":"413","author":"Mayne","year":"2021","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jchromb.2025.124541_bb0095","doi-asserted-by":"crossref","first-page":"9497","DOI":"10.1007\/s00216-013-7391-2","article-title":"A sensitive and selective LC-differential mobility-mass spectrometric analysis of allopregnanolone and pregnanolone in human plasma","volume":"405","author":"Jin","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jchromb.2025.124541_bb0100","doi-asserted-by":"crossref","first-page":"225","DOI":"10.33549\/physiolres.932077","article-title":"Reduced progesterone metabolites in human late pregnancy","volume":"60","author":"Hill","year":"2011","journal-title":"Physiol. Res."},{"key":"10.1016\/j.jchromb.2025.124541_bb0105","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/1471-2393-13-95","article-title":"A randomised controlled trial comparing standard intensive management of reduced fetal movements after 36 weeks gestation \u2013 a feasibility study","volume":"13","author":"Heazell","year":"2013","journal-title":"BMC Pregnancy Childbirth"},{"key":"10.1016\/j.jchromb.2025.124541_bb0110","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s40814-020-0561-z","article-title":"Standard care informed by the result of a placental growth factor blood test versus standard care alone in women with reduced fetal movements at or after 36 weeks\u2019 gestation: a pilot randomised controlled trial","volume":"6","author":"Armstrong-Buisseret","year":"2020","journal-title":"Pilot Feasibility Stud"},{"key":"10.1016\/j.jchromb.2025.124541_bb0115","author":"Food and Drug Administration"},{"key":"10.1016\/j.jchromb.2025.124541_bb0120","author":"Intergrowth-21st"},{"key":"10.1016\/j.jchromb.2025.124541_bb0125","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jpba.2017.02.007","article-title":"Stress-induced changes of neurosteroid profiles in rat brain and plasma under immobilised condition","volume":"138","author":"Park","year":"2017","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jchromb.2025.124541_bb0130","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.steroids.2016.06.011","article-title":"Precise and accurate assay of pregnenolone and five other neurosteroids in monkey brain tissue by LC-MS","volume":"113","author":"Dury","year":"2016","journal-title":"Steroids"},{"key":"10.1016\/j.jchromb.2025.124541_bb0135","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.psyneuen.2016.05.010","article-title":"Peripartum neuroactive steroid and gamma-aminobutyric acid profiles in women at-risk for postpartum depression","volume":"70","author":"Deligiannidis","year":"2016","journal-title":"Psychoneuroendocrinology"},{"key":"10.1016\/j.jchromb.2025.124541_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2021.122358","article-title":"Simultaneous quantification of progestogens in plasma and serum by UHPL-HRMS employing multiplexed targeted single ion monitoring","volume":"232","author":"Rehm","year":"2021","journal-title":"Talanta"},{"key":"10.1016\/j.jchromb.2025.124541_bb0145","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.psyneuen.2006.11.008","article-title":"Long-term stability of maternal prenatal steroid hormones from the National Collaborative Perinatal Project: Still valid after all these years","volume":"32","author":"Stroud","year":"2007","journal-title":"Psychoneuroendocrinology"},{"key":"10.1016\/j.jchromb.2025.124541_bb0150","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1080\/02841860701592400","article-title":"Effect of long-term storage on hormone measurements in samples from pregnant women: The experience of the Finnish maternity cohort","volume":"47","author":"Holl","year":"2008","journal-title":"Acta Oncol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0155","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.jsbmb.2006.10.010","article-title":"Circulating levels of pregnanolone isomers during the third trimester of human pregnancy","volume":"105","author":"Hill","year":"2007","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0160","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.cbi.2014.11.012","article-title":"Important roles of the AKR1C2 and SRD5A1 enzymes in progesterone metabolism in endometrial cancer model cell lines","volume":"234","author":"Sinreih","year":"2015","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.jchromb.2025.124541_bb0165","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1507\/endocrj.49.539","article-title":"Progesterone metabolism in human leukemic monoblast U937 cells","volume":"49","author":"Suzuki","year":"2002","journal-title":"Endocr. J."},{"key":"10.1016\/j.jchromb.2025.124541_bb0170","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1210\/jcem.85.7.6675","article-title":"Serum allopregnanolone levels in pregnant women: changes during pregnancy, at delivery, and in hypertensive patients","volume":"85","author":"Luisi","year":"2000","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"10.1016\/j.jchromb.2025.124541_bb0175","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.placenta.2018.07.004","article-title":"Abnormal steroidogenesis and aromatase activity in preeclampsia","volume":"69","author":"Berkane","year":"2018","journal-title":"Placenta"},{"key":"10.1016\/j.jchromb.2025.124541_bb0180","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1016\/j.placenta.2009.08.008","article-title":"Changes in the metabolic footprint of placental explant-conditioned culture medium identifies metabolic disturbances related to hypoxia and pre-eclampsia","volume":"30","author":"Dunn","year":"2009","journal-title":"Placenta"},{"key":"10.1016\/j.jchromb.2025.124541_bb0185","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.3390\/ijms21031043","article-title":"The metabolomic signature of the placenta in spontaneous preterm birth","volume":"21","author":"Elshenawy","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jchromb.2025.124541_bb0190","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/9362820","article-title":"Maternal plasma metabolomic profiles in spontaneous preterm birth: Preliminary results","author":"Lizewska","year":"2018","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.jchromb.2025.124541_bb0195","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1080\/09513590500361747","article-title":"Sellers et al, 3\u03b1-reduced neuroactive steroids and their precursors during pregnancy and the postpartum period","volume":"21","author":"Gilbert Evans","year":"2005","journal-title":"Gynecol. Endocrinol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0200","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1111\/j.1365-2826.2011.02219.x","article-title":"Inhibition of 5\u03b1-reductase activity in late pregnancy decreases gestational length and fecundity and impairs object memory and central progestogen milieu of juvenile rat offspring","volume":"23","author":"Paris","year":"2011","journal-title":"J. Neuroendocrinol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0205","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.lfs.2018.10.033","article-title":"Profiling of GABAA and GABAB receptor expression in the myometrium of the human uterus","volume":"214","author":"Soderhielm","year":"2018","journal-title":"Life Sci."},{"key":"10.1016\/j.jchromb.2025.124541_bb0210","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1385\/ENDO:15:1:063","article-title":"Nongenomic action of steroids in myometrial contractility","volume":"15","author":"Perusquia","year":"2001","journal-title":"Endocrine"},{"key":"10.1016\/j.jchromb.2025.124541_bb0215","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1111\/j.1365-2826.2012.02295.x","article-title":"Allopregnanolone and induction of endogenous opioid inhibition of oxytocin responses to immune stress in pregnant rats","volume":"24","author":"Brunton","year":"2012","journal-title":"J. Neuroendocrinol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0220","first-page":"1","article-title":"A nested case-control study of allopregnanolone and preterm birth in the healthy start cohort","volume":"7","author":"Mayne","year":"2023","journal-title":"J Endocr Soc"},{"key":"10.1016\/j.jchromb.2025.124541_bb0225","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.pnpbp.2013.09.005","article-title":"Multifunctional aspects of allopregnanolone in stress and related disorders","volume":"48","author":"Bali","year":"2014","journal-title":"Prog. Neuro-Psychopharmacol. Biol. Psychiatry"},{"key":"10.1016\/j.jchromb.2025.124541_bb0230","doi-asserted-by":"crossref","unstructured":"A. Concas, M.C. Mostallino, P. Porcu et al, Role of brain allopregnanolone in the plasticity of \u03b3-aminobutyric acid type a receptor in rat brain during pregnancy and after delivery, Proc. Natl. Acad. Sci., 95 (1998) 13284\u201313289, https:\/\/doi.org.uk\/https:\/\/doi.org\/10.1073\/pnas.95.22.13284.","DOI":"10.1073\/pnas.95.22.13284"},{"key":"10.1016\/j.jchromb.2025.124541_bb0235","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0014-2999(99)00232-0","article-title":"Physiological modulation of GABAA receptor plasticity by progesterone metabolites","volume":"375","author":"Concas","year":"1999","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.jchromb.2025.124541_bb0240","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1016\/S0140-6736(18)31551-4","article-title":"Brexanolone injection in post-partum depression: Two multicentre, double-blind, randomised, placebo-controlled, phase 3 trials","volume":"392","author":"Meltzer-Brody","year":"2018","journal-title":"Lancet"}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023225000935?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023225000935?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T19:02:19Z","timestamp":1746817339000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023225000935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":48,"alternative-id":["S1570023225000935"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2025.124541","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Profiling neuroactive steroids in pregnancy. A non-derivatised liquid chromatography tandem mass spectrometry method for the quantitation of allopregnanolone and four related isomers in maternal serum.","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2025.124541","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"124541"}}