{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T23:57:52Z","timestamp":1772495872201,"version":"3.50.1"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100011447","name":"Science and Technology Department of Henan Province","doi-asserted-by":"publisher","award":["242102110071"],"award-info":[{"award-number":["242102110071"]}],"id":[{"id":"10.13039\/501100011447","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006407","name":"Henan Province Natural Science Foundation","doi-asserted-by":"publisher","award":["162300410228"],"award-info":[{"award-number":["162300410228"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography B"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.jchromb.2025.124646","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T11:55:32Z","timestamp":1747396532000},"page":"124646","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Optimized plasmid extraction through controlled temperature, prolonged alkaline lysis, and gentle mixing"],"prefix":"10.1016","volume":"1262","author":[{"given":"Ruijie","family":"Hao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengzhen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjie","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengpeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bijie","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefeng","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jchromb.2025.124646_bb0005","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.chroma.2007.06.061","article-title":"Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process","volume":"1164","author":"Duarte","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2025.124646_bb0010","series-title":"Plasmid analysis, Molecular bacteriology: protocols and clinical applications","first-page":"51","author":"Johnson","year":"1998"},{"key":"10.1016\/j.jchromb.2025.124646_bb0015","doi-asserted-by":"crossref","first-page":"3088","DOI":"10.1038\/nprot.2006.452","article-title":"A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis","volume":"1","author":"Zhu","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jchromb.2025.124646_bb0020","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1006\/abio.1994.1542","article-title":"A high-throughput plasmid DNA preparation method","volume":"223","author":"Huang","year":"1994","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2025.124646_bb0025","first-page":"57","article-title":"To plate or to simply unfreeze, that is the question for optimal plasmid extraction","volume":"32","author":"Thean","year":"2021","journal-title":"Journal of Biomolecular Techniques: JBT"},{"key":"10.1016\/j.jchromb.2025.124646_bb0030","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ab.2018.11.005","article-title":"A modified method for plasmid extraction from lactobacillus plantarum contained lysozyme removal step","volume":"566","author":"Yao","year":"2019","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2025.124646_bb0035","first-page":"135","article-title":"Isolation of plasmid DNA from bacteria, methods in enzymology","author":"Andreou","year":"2013","journal-title":"Elsevier"},{"key":"10.1016\/j.jchromb.2025.124646_bb0040","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s00253-007-0925-9","article-title":"An automated process to extract plasmid DNA by alkaline lysis","volume":"75","author":"Li","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jchromb.2025.124646_bb0045","series-title":"Preparing Plasmid DNA from bacteria","author":"Figueroa-Bossi","year":"2022"},{"key":"10.1016\/j.jchromb.2025.124646_bb0050","series-title":"Preparation of bacterial plasmid DNA, Current protocols in immunology","volume":"27","author":"Engebrecht","year":"1998"},{"key":"10.1016\/j.jchromb.2025.124646_bb0055","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/nprot.2007.526","article-title":"A continuous process to extract plasmid DNA based on alkaline lysis","volume":"3","author":"Li","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jchromb.2025.124646_bb0060","series-title":"Isolation of Plasmid DNA by Alkaline Lysis, DNA and RNA Isolation Techniques for Non-Experts","first-page":"55","author":"Gautam","year":"2022"},{"key":"10.1016\/j.jchromb.2025.124646_bb0065","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1016\/j.vaccine.2009.10.061","article-title":"Preparation of pharmaceutical-grade plasmid DNA using methacrylate monolithic columns","volume":"28","author":"Smrekar","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.jchromb.2025.124646_bb0070","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/bit.21142","article-title":"Alkaline-cell lysis through in-line static mixer reactor for the production of plasmid DNA for gene therapy","volume":"96","author":"Chamsart","year":"2007","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jchromb.2025.124646_bb0075","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s00253-009-2088-3","article-title":"Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction","volume":"84","author":"Tschapalda","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jchromb.2025.124646_bb0080","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0167-7799(98)01291-8","article-title":"Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks","volume":"17","author":"Prazeres","year":"1999","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.jchromb.2025.124646_bb0085","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1002\/bit.28198","article-title":"New horizons in developing cell lysis methods: a review","volume":"119","author":"Danaeifar","year":"2022","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jchromb.2025.124646_bb0090","doi-asserted-by":"crossref","first-page":"533","DOI":"10.18782\/2320-7051.6988","article-title":"A comparative study on different plasmid isolation procedures","volume":"6","author":"Roy","year":"2018","journal-title":"Int J Pure Appl Biosci"},{"key":"10.1016\/j.jchromb.2025.124646_bb0095","doi-asserted-by":"crossref","first-page":"498","DOI":"10.3390\/mi12050498","article-title":"Review of microfluidic methods for cellular lysis","volume":"12","author":"Grigorov","year":"2021","journal-title":"Micromachines"},{"key":"10.1016\/j.jchromb.2025.124646_bb0100","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/bit.20114","article-title":"Impact of engineering flow conditions on plasmid DNA yield and purity in chemical cell lysis operations","volume":"87","author":"Meacle","year":"2004","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jchromb.2025.124646_bb0105","series-title":"Optimisation of an alkaline lysis process for a plug-and play plasmid DNA production","author":"Almulla","year":"2022"},{"key":"10.1016\/j.jchromb.2025.124646_bb0110","series-title":"Plasmid DNA purification, The Journal of Gene Medicine: A cross-disciplinary journal for research on the science of gene transfer and its clinical applications","first-page":"S54","volume":"6","author":"Stadler","year":"2004"},{"key":"10.1016\/j.jchromb.2025.124646_bb0115","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1002\/(SICI)1097-0290(19981220)60:6<768::AID-BIT13>3.0.CO;2-D","article-title":"Time course of SDS\u2013alkaline lysis of recombinant bacterial cells for plasmid release","volume":"60","author":"Ciccolini","year":"1998","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jchromb.2025.124646_bb0120","article-title":"A home made kit for plasmid DNA mini-preparation","volume":"2","author":"Kotchoni","year":"2003","journal-title":"Afr. J. Biotechnol."},{"key":"10.1016\/j.jchromb.2025.124646_bb0125","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1006\/abio.1993.1346","article-title":"A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli","volume":"212","author":"Feliciello","year":"1993","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2025.124646_bb0130","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ab.2012.03.013","article-title":"Application of magnetic hydroxyapatite nanoparticles for solid phase extraction of plasmid DNA","volume":"425","author":"Shan","year":"2012","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jchromb.2025.124646_bb0135","first-page":"558","article-title":"A novel method of plasmid isolation using laundry detergent","volume":"49","author":"Yadav","year":"2011","journal-title":"Indian J. Exp. Biol."},{"key":"10.1016\/j.jchromb.2025.124646_bb0140","series-title":"Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Minipreps","volume":"2016","author":"Green","year":"2016"},{"key":"10.1016\/j.jchromb.2025.124646_bb0145","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.aca.2014.06.002","article-title":"Novel polymeric ionic liquid microspheres with high exchange capacity for fast extraction of plasmid DNA","volume":"837","author":"Wang","year":"2014","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jchromb.2025.124646_bb0150","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1007\/s10529-011-0612-x","article-title":"Large-scale plasmid preparation for transient gene expression","volume":"33","author":"Cheng","year":"2011","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.jchromb.2025.124646_bb0155","series-title":"Preparation of Plasmid Dna by Alkaline Lysis with Sodium Dodecyl Sulfate: Maxipreps","volume":"2018","author":"Green","year":"2018"},{"key":"10.1016\/j.jchromb.2025.124646_bb0160","series-title":"Large-scale preparation of plasmid DNA, Current protocols in molecular biology","volume":"41","author":"Heilig","year":"1998"},{"key":"10.1016\/j.jchromb.2025.124646_bb0165","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1007\/s00232-013-9580-5","article-title":"Comparison of alkaline lysis with electroextraction and optimization of electric pulses to extract plasmid DNA from Escherichia coli","volume":"246","author":"Haberl","year":"2013","journal-title":"J. Membr. Biol."},{"key":"10.1016\/j.jchromb.2025.124646_bb0170","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/bit.10046","article-title":"The impact of fluid-dynamic-generated stresses on chDNA and pDNA stability during alkaline cell lysis for gene therapy products","volume":"75","author":"Chamsart","year":"2001","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jchromb.2025.124646_bb0175","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1042\/BA20030002","article-title":"Optimizing alkaline lysis for DNA plasmid recovery","volume":"37","author":"Clemson","year":"2003","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.1016\/j.jchromb.2025.124646_bb0180","series-title":"A Cell Lysis Reactor for the Production of Plasmid DNA from Recombinant E. coli for Gene Therapy","author":"Chamsart","year":"2001"},{"key":"10.1016\/j.jchromb.2025.124646_bb0185","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1023\/A:1013106032341","article-title":"Extraction of plasmid DNA using reactor scale alkaline lysis and selective precipitation for scalable transient transfection","volume":"35","author":"Wright","year":"2001","journal-title":"Cytotechnology"},{"key":"10.1016\/j.jchromb.2025.124646_bb0190","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1007\/s10529-012-0994-4","article-title":"Plasmid size can affect the ability of Escherichia coli to produce high-quality plasmids","volume":"34","author":"Yang","year":"2012","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.jchromb.2025.124646_bb0195","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.chroma.2003.10.028","article-title":"Extraction of plasmid DNA from Escherichia coli cell lysate in a thermoseparating aqueous two-phase system","volume":"1024","author":"Kepka","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jchromb.2025.124646_bb0200","doi-asserted-by":"crossref","first-page":"5771","DOI":"10.1093\/nar\/22.25.5771","article-title":"Simple methods for preparation of plasmid DNA yielding long and accurate sequence data","volume":"22","author":"Kovalenko","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jchromb.2025.124646_bb0205","first-page":"99","article-title":"Plasmid preparation methods for DNA sequencing, DNA sequencing: optimizing the process and analysis","author":"Flick","year":"2005","journal-title":"Jones and Bartlett Publishers, Sudbury MA"},{"key":"10.1016\/j.jchromb.2025.124646_bb0210","doi-asserted-by":"crossref","DOI":"10.2144\/02323pf02","article-title":"High-speed plasmid isolation using 96-well, size-exclusion filter plates","volume":"32","author":"Harris","year":"2002","journal-title":"Biotechniques"},{"key":"10.1016\/j.jchromb.2025.124646_bb0215","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12985-016-0552-0","article-title":"Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA","volume":"13","author":"Lemire","year":"2016","journal-title":"Virol. J."},{"key":"10.1016\/j.jchromb.2025.124646_bb0220","series-title":"E. coli Plasmid Vectors: Methods and Applications","first-page":"75","article-title":"Isolation of plasmids from E. Coli by alkaline lysis","author":"Ehrt","year":"2003"},{"key":"10.1016\/j.jchromb.2025.124646_bb0225","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1085\/jgp.49.6.103","article-title":"Physical and topological properties of circular DNA","volume":"49","author":"Vinograd","year":"1966","journal-title":"J. Gen. Physiol."},{"key":"10.1016\/j.jchromb.2025.124646_bb0230","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/BF03245921","article-title":"Thermodynamics of protein denaturation by sodium dodecyl sulfate","volume":"2","author":"Moosavi-Movahedi","year":"2005","journal-title":"J. Iran. Chem. Soc."}],"container-title":["Journal of Chromatography B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023225002004?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1570023225002004?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T22:11:59Z","timestamp":1762553519000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1570023225002004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":46,"alternative-id":["S1570023225002004"],"URL":"https:\/\/doi.org\/10.1016\/j.jchromb.2025.124646","relation":{},"ISSN":["1570-0232"],"issn-type":[{"value":"1570-0232","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimized plasmid extraction through controlled temperature, prolonged alkaline lysis, and gentle mixing","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography B","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jchromb.2025.124646","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"124646"}}