{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T14:05:33Z","timestamp":1789221933606,"version":"build-2803163510"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013414","name":"Regional Council of Pays de la Loire","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013414","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022077","name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022077","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Colloid and Interface Science"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.jcis.2026.140823","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T15:23:08Z","timestamp":1780154588000},"page":"140823","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Complexation of alginate with linear and branched polyethyleneimine"],"prefix":"10.1016","volume":"722","author":[{"given":"Asna","family":"Vakeri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Bouchoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Boire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Paul","family":"Chapel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joelle","family":"Davy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascaline","family":"Hamon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u00efd","family":"Bouhallab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Renard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcis.2026.140823_bb0005","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.cis.2016.10.004","article-title":"Structure, thermodynamic and kinetic signatures of a synthetic polyelectrolyte coacervating system","volume":"239","author":"Liu","year":"2017","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.1016\/j.jcis.2026.140823_bb0010","doi-asserted-by":"crossref","first-page":"7630","DOI":"10.1039\/C7OB01314K","article-title":"The effect of comb architecture on complex coacervation","volume":"15","author":"Johnston","year":"2017","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.jcis.2026.140823_bb0015","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.ijbiomac.2018.05.194","article-title":"Conformational change of lysozyme on the interaction with gene carrier polyethyleneimine","volume":"117","author":"Wang","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.jcis.2026.140823_bb0020","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ijbiomac.2010.04.003","article-title":"Polyethyleneimine-protein interactions and implications on protein stability","volume":"47","author":"Mazzaferro","year":"2010","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.jcis.2026.140823_bb0025","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1039\/C6SM01801G","article-title":"Complexation and synergistic boundary lubrication of porcine gastric mucin and branched poly(ethyleneimine) in neutral aqueous solution","volume":"13","author":"Patil","year":"2017","journal-title":"Soft Matter"},{"key":"10.1016\/j.jcis.2026.140823_bb0030","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/10601320500437045","article-title":"Solid-state polyelectrolyte complexes of branched poly(ethylenimine) and sodium lauryl sulfate","volume":"43","author":"Kharas","year":"2006","journal-title":"Journal of Macromolecular Science - Pure Appl. Chem."},{"key":"10.1016\/j.jcis.2026.140823_bb0035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1103\/PhysRevE.84.021805","article-title":"Phase behavior and structure of stable complexes between a long polyanion and a branched polycation","volume":"84","author":"Mengarelli","year":"2011","journal-title":"Phys. Rev. E Stat. Nonlin. Soft Matter Phys."},{"key":"10.1016\/j.jcis.2026.140823_bb0040","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.ijpharm.2012.06.052","article-title":"Proteins, polysaccharides, and their complexes used as stabilizers for emulsions: alternatives to synthetic surfactants in the pharmaceutical field?","volume":"436","author":"Bouyer","year":"2012","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.jcis.2026.140823_bb0045","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.progpolymsci.2011.06.003","article-title":"Alginate: properties and biomedical applications","volume":"37","author":"Lee","year":"2012","journal-title":"Progress in Polymer Science (Oxford)"},{"key":"10.1016\/j.jcis.2026.140823_bb0050","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.1021\/acs.biomac.3c00171","article-title":"Effects of salt on phase behavior and rheological properties of alginate-chitosan polyelectrolyte complexes","volume":"24","author":"Varadarajan","year":"2023","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.jcis.2026.140823_bb0055","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.carbpol.2013.09.025","article-title":"The effect of alginate on DNA delivery from layer-by-layer assembled films","volume":"101","author":"Hu","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.jcis.2026.140823_bb0060","doi-asserted-by":"crossref","first-page":"779","DOI":"10.3727\/000000003108747253","article-title":"The effects of poly(ethyleneimine) (PEI) molecular weight on reinforcement of alginate hydrogels","volume":"12","author":"Kong","year":"2003","journal-title":"Cell Transplant."},{"key":"10.1016\/j.jcis.2026.140823_bb0065","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.cis.2016.08.005","article-title":"Liquid-liquid and liquid-solid phase separation in protein-polyelectrolyte systems","volume":"239","author":"Comert","year":"2017","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.1016\/j.jcis.2026.140823_bb0070","doi-asserted-by":"crossref","DOI":"10.3390\/polym13213848","article-title":"Interfacial behavior of solid-and liquid-like polyelectrolyte complexes as a function of charge stoichiometry","volume":"13","author":"Li","year":"2021","journal-title":"Polymers (Basel)."},{"key":"10.1016\/j.jcis.2026.140823_bb0075","doi-asserted-by":"crossref","first-page":"3108","DOI":"10.1021\/ma500500q","article-title":"The polyelectrolyte complex\/coacervate continuum","volume":"47","author":"Wang","year":"2014","journal-title":"Macromolecules"},{"key":"10.1016\/j.jcis.2026.140823_bb0080","article-title":"Alginate-graft-PEI as a gene delivery vector with high efficiency and low cytotoxicity alginate-graft-PEI as a gene delivery vector with high","volume":"5063","author":"He","year":"2012","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"10.1016\/j.jcis.2026.140823_bb0085","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.ijpharm.2009.10.041","article-title":"PEI-alginate nanocomposites\u00a0: efficient non-viral vectors for nucleic acids","volume":"385","author":"Patnaik","year":"2010","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.jcis.2026.140823_bb0090","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.jconrel.2006.06.025","article-title":"PEI-alginate nanocomposites as efficient in vitro gene transfection agents","volume":"114","author":"Patnaik","year":"2006","journal-title":"J. Control. Release"},{"key":"10.1016\/j.jcis.2026.140823_bb0095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-021-97228-0","article-title":"Polyethyleneimine impregnated alginate capsule as a high capacity sorbent for the recovery of monovalent and trivalent gold","volume":"11","author":"Dangi","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jcis.2026.140823_bb0100","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1002\/mabi.201600214","article-title":"Non-covalently stabilized alginate hydrogels as functional cell scaffold material","volume":"16","author":"K\u00fchn","year":"2016","journal-title":"Macromol. Biosci."},{"key":"10.1016\/j.jcis.2026.140823_bb0105","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1021\/j100838a022","article-title":"Phase separation in polyelectrolyte systems. I. Complex coacervates of gelatin","volume":"64","author":"Veis","year":"1960","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.jcis.2026.140823_bb0110","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/12_2012_171","article-title":"Aggregation of charged colloidal particles","volume":"255","author":"Lebovka","year":"2014","journal-title":"Adv. Polym. Sci."},{"key":"10.1016\/j.jcis.2026.140823_bb0115","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.cis.2010.10.001","article-title":"Protein\/polysaccharide complexes and coacervates in food systems","volume":"167","author":"Schmitt","year":"2011","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.1016\/j.jcis.2026.140823_bb0120","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s11483-020-09645-9","article-title":"Optimization of a droplet-based Millifluidic device to investigate the phase behavior of biopolymers, including viscous conditions","volume":"15","author":"Amine","year":"2020","journal-title":"Food Biophys."},{"key":"10.1016\/j.jcis.2026.140823_bb0125","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.foodhyd.2017.03.035","article-title":"Droplets-based millifluidic for the rapid determination of biopolymers phase diagrams","volume":"70","author":"Amine","year":"2017","journal-title":"Food Hydrocoll."},{"key":"10.1016\/j.jcis.2026.140823_bb0130","doi-asserted-by":"crossref","first-page":"10950","DOI":"10.1021\/la7008545","article-title":"Polyelectrolyte complexes from polysaccharides: formation and stoichiometry monitoring","volume":"23","author":"Drogoz","year":"2007","journal-title":"Langmuir"},{"key":"10.1016\/j.jcis.2026.140823_bb0135","series-title":"Complexes de poly\u00e9lectrolytes: Morphologies, cin\u00e9tique et thermodynamique d'assemblage","author":"Haddou","year":"2017"},{"key":"10.1016\/j.jcis.2026.140823_bb0140","doi-asserted-by":"crossref","first-page":"9496","DOI":"10.1039\/C4SM01461H","article-title":"Evidence of a two-step process and pathway dependency in the thermodynamics of poly(diallyldimethylammonium chloride)\/poly(sodium acrylate) complexation","volume":"10","author":"Vitorazi","year":"2014","journal-title":"Soft Matter"},{"key":"10.1016\/j.jcis.2026.140823_bb0145","doi-asserted-by":"crossref","DOI":"10.3390\/polym14122404","article-title":"Complexation in aqueous solution of a hydrophobic polyanion (PSSNa) bearing different charge densities with a hydrophilic polycation (PDADMAC)","volume":"14","author":"Jemili","year":"2022","journal-title":"Polymers (Basel)."},{"key":"10.1016\/j.jcis.2026.140823_bb0150","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.jcis.2023.04.030","article-title":"Specific ion effects on the aggregation of polysaccharide-based polyelectrolyte complex particles induced by monovalent ions within Hofmeister series","volume":"643","author":"Yamazaki","year":"2023","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.jcis.2026.140823_bb0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodhyd.2024.110359","article-title":"Coacervation and aggregation in lysozyme \/ alginate mixtures","volume":"156","author":"Vakeri","year":"2024","journal-title":"Food Hydrocoll."},{"key":"10.1016\/j.jcis.2026.140823_bb0160","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.foodhyd.2019.01.026","article-title":"Associative properties of rapeseed napin and pectin: competition between liquid-liquid and liquid-solid phase separation","volume":"92","author":"Amine","year":"2019","journal-title":"Food Hydrocoll."},{"key":"10.1016\/j.jcis.2026.140823_bb0165","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodhyd.2021.107339","article-title":"Dynamic equilibrium of \u03b2-conglycinin\/lysozyme heteroprotein complex coacervates","volume":"124","author":"Zheng","year":"2022","journal-title":"Food Hydrocoll."},{"key":"10.1016\/j.jcis.2026.140823_bb0170","unstructured":"H. Li, L. Porcar, A. Bentaleb, C. Schatz, J. Chapel, Concentration-dependent coacervate characteristics of a complex, (n.d.)."},{"key":"10.1016\/j.jcis.2026.140823_bb0175","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0927-7765(00)00200-9","article-title":"Study of \u03b2-lactoglobulin\/acacia gum complex coacervation by diffusing-wave spectroscopy and confocal scanning laser microscopy","volume":"20","author":"Schmitt","year":"2001","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.jcis.2026.140823_bb0180","doi-asserted-by":"crossref","first-page":"15947","DOI":"10.1021\/la302729r","article-title":"Thermodynamic characterization of polypeptide complex coacervation","volume":"28","author":"Priftis","year":"2012","journal-title":"Langmuir"},{"key":"10.1016\/j.jcis.2026.140823_bb0185","doi-asserted-by":"crossref","DOI":"10.1016\/j.cossms.2020.100897","article-title":"Expanding the structural diversity of polyelectrolyte complexes and polyzwitterions","volume":"25","author":"Neitzel","year":"2021","journal-title":"Curr. Opin. Solid State Mater. Sci."},{"key":"10.1016\/j.jcis.2026.140823_bb0190","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1021\/j100889a003","article-title":"A conductometric study of polycation-polyanion reactions in dilute aqueous solution","volume":"69","author":"Michaels","year":"1965","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.jcis.2026.140823_bb0195","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0091-679X(07)84004-0","article-title":"Isothermal titration calorimetry: experimental design, data analysis, and probing macromolecule\/ligand binding and kinetic interactions","volume":"84","author":"Freyer","year":"2008","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.jcis.2026.140823_bb0200","doi-asserted-by":"crossref","first-page":"7859","DOI":"10.1021\/acs.jpcb.4c02484","article-title":"Mixing order asymmetry in nanoparticle-polymer complexation and precipitation revealed by isothermal titration calorimetry","volume":"128","author":"Vitorazi","year":"2024","journal-title":"J. Phys. Chem. B"},{"key":"10.1016\/j.jcis.2026.140823_bb0205","doi-asserted-by":"crossref","first-page":"2510","DOI":"10.1021\/acs.langmuir.9b03216","article-title":"Effect of pH on the complex coacervation and on the formation of layers of sodium alginate and PDADMAC","volume":"36","author":"Dos Santos de Macedo","year":"2020","journal-title":"Langmuir"},{"key":"10.1016\/j.jcis.2026.140823_bb0210","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1021\/jacs.5b11878","article-title":"Driving forces for oppositely charged Polyion Association in Aqueous Solutions: enthalpic, entropic, but not electrostatic","volume":"138","author":"Fu","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jcis.2026.140823_bb0215","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1021\/acsmacrolett.4c00167","article-title":"Impact of a lightly branched star polyelectrolyte architecture on polyelectrolyte complexes","volume":"13","author":"Stevens","year":"2024","journal-title":"ACS Macro Lett."},{"key":"10.1016\/j.jcis.2026.140823_bb0220","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.jcis.2024.06.062","article-title":"Distribution of polyelectrolytes and counterions upon polyelectrolyte complexation","volume":"672","author":"Allegri","year":"2024","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.jcis.2026.140823_bb0225","doi-asserted-by":"crossref","first-page":"6403","DOI":"10.1021\/acs.biomac.4c00447","article-title":"Structure and dynamics of water in polysaccharide (alginate) solutions and gels explained by the Core-Shell model","volume":"25","author":"Agles","year":"2024","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.jcis.2026.140823_bb0230","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1021\/acs.biomac.1c01591","article-title":"Competing effects of hydration and cation complexation in single-chain alginate","volume":"23","author":"Li","year":"2022","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.jcis.2026.140823_bb0235","doi-asserted-by":"crossref","first-page":"7393","DOI":"10.1039\/D5SM00310E","article-title":"Revisiting the solution properties of sodium alginate in aqueous media","volume":"21","author":"Yang","year":"2025","journal-title":"Soft Matter"},{"key":"10.1016\/j.jcis.2026.140823_bb0240","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-21181-9","article-title":"Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions","volume":"12","author":"Krainer","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jcis.2026.140823_bb0245","doi-asserted-by":"crossref","first-page":"16373","DOI":"10.1021\/jp106610t","article-title":"Influence of the formulation process in electrostatic assembly of nanoparticles and macromolecules in aqueous solution: the interaction pathway","volume":"114","author":"Qi","year":"2010","journal-title":"J. Phys. Chem. C"},{"key":"10.1016\/j.jcis.2026.140823_bb0250","article-title":"Concentration-dependent characteristics of a complex coacervate","author":"Li","year":"2022","journal-title":"ChemRxiv preprint"}],"container-title":["Journal of Colloid and Interface Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021979726010003?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021979726010003?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T13:06:39Z","timestamp":1789218399000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021979726010003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":50,"alternative-id":["S0021979726010003"],"URL":"https:\/\/doi.org\/10.1016\/j.jcis.2026.140823","relation":{},"ISSN":["0021-9797"],"issn-type":[{"value":"0021-9797","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Complexation of alginate with linear and branched polyethyleneimine","name":"articletitle","label":"Article Title"},{"value":"Journal of Colloid and Interface Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcis.2026.140823","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"140823"}}