{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:52:26Z","timestamp":1790236346433,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Controlled Release"],"published-print":{"date-parts":[[2004,4]]},"DOI":"10.1016\/j.jconrel.2004.01.022","type":"journal-article","created":{"date-parts":[[2004,3,25]],"date-time":"2004-03-25T13:59:20Z","timestamp":1080223160000},"page":"309-323","source":"Crossref","is-referenced-by-count":85,"title":["Hydrophobized dextran-spermine conjugate as potential vector for in vitro gene transfection"],"prefix":"10.1016","volume":"96","author":[{"given":"Tony","family":"Azzam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagit","family":"Eliyahu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arik","family":"Makovitzki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Linial","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham J","family":"Domb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.jconrel.2004.01.022_BIB1","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1021\/bc010033j","article-title":"In vitro cationic lipid-mediated gene delivery with fluorinated glycerophosphoethanolamine helper lipids","volume":"12","author":"Gaucheron","year":"2001","journal-title":"Bioconjugate Chem."},{"issue":"9","key":"10.1016\/j.jconrel.2004.01.022_BIB2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S1461-5347(99)00186-8","article-title":"Taking polycation gene delivery systems from in vitro to in vivo","volume":"2","author":"Kabanov","year":"1999","journal-title":"Pharm. Sci. Technol. Today"},{"key":"10.1016\/j.jconrel.2004.01.022_BIB3","series-title":"Polymers in Gene Therapy: Frontiers in Biological Polymer Application","author":"Domb","year":"1999"},{"issue":"3","key":"10.1016\/j.jconrel.2004.01.022_BIB4","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1073\/pnas.031577698","article-title":"Polymer-based gene delivery with low cytotoxicity by a unique balance of side-chain termini","volume":"98","author":"Putnam","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"9","key":"10.1016\/j.jconrel.2004.01.022_BIB5","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.1246\/bcsj.68.2709","article-title":"Preparation and characterization of DNA\u2013lipoglutamate complexes","volume":"68","author":"Sato","year":"1995","journal-title":"Bull. Chem. Soc. Jpn."},{"issue":"16","key":"10.1016\/j.jconrel.2004.01.022_BIB6","doi-asserted-by":"crossref","first-page":"7297","DOI":"10.1073\/pnas.92.16.7297","article-title":"A versatile vector for gene and oligonucleotide transfer into cells in culture and in-vivo-polyethylenimine","volume":"92","author":"Boussif","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"4","key":"10.1016\/j.jconrel.2004.01.022_BIB7","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1021\/bc990143e","article-title":"Steric stabilization of poly-l-lysine\/DNA complexes by the covalent attachment of semitelechelic poly N-(2-hydroxypropyl)methacrylamide","volume":"11","author":"Oupicky","year":"2000","journal-title":"Bioconjugate Chem."},{"issue":"9","key":"10.1016\/j.jconrel.2004.01.022_BIB8","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/S0142-9612(99)00229-X","article-title":"Application of membrane-based dendrimer\/DNA complexes for solid phase transfection in vitro and in vivo","volume":"21","author":"Bielinska","year":"2000","journal-title":"Biomaterials"},{"issue":"5","key":"10.1016\/j.jconrel.2004.01.022_BIB9","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1023\/A:1016039330870","article-title":"Polyvinyl derivatives as novel interactive polymers for controlled gene delivery to muscle","volume":"13","author":"Mumper","year":"1996","journal-title":"Pharm. Res."},{"issue":"1\u20133","key":"10.1016\/j.jconrel.2004.01.022_BIB10","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0168-3659(97)00252-6","article-title":"DNA-polycation nanospheres as non-viral gene delivery vehicles","volume":"53","author":"Leong","year":"1998","journal-title":"J. Control. Release"},{"issue":"18","key":"10.1016\/j.jconrel.2004.01.022_BIB11","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.1002\/1521-3773(20010917)40:18<3364::AID-ANIE3364>3.0.CO;2-S","article-title":"Atomic force microscope studies on condensation of plasmid DNA with functionalized fullerenes","volume":"40","author":"Isobe","year":"2001","journal-title":"Angew. Chem. Int. Ed."},{"issue":"5","key":"10.1016\/j.jconrel.2004.01.022_BIB12","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1021\/bc0000177","article-title":"pH-sensitive cationic polymer gene delivery vehicle: N-Ac-poly(l-histidine)-graft-poly(l-lysine) comb shaped polymer","volume":"11","author":"Benns","year":"2000","journal-title":"Bioconjugate Chem."},{"issue":"9","key":"10.1016\/j.jconrel.2004.01.022_BIB13","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1023\/A:1011933117509","article-title":"Target specific optimization of cationic lipid-based systems for pulmonary gene therapy","volume":"15","author":"Deshpande","year":"1998","journal-title":"Pharm. Res."},{"issue":"1","key":"10.1016\/j.jconrel.2004.01.022_BIB14","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1021\/ja016440n","article-title":"Laterally stabilized complexes of DNA with linear reducible polycations: strategy for triggered intracellular activation of DNA delivery vectors","volume":"124","author":"Oupicky","year":"2002","journal-title":"J. Am. Chem. Soc."},{"issue":"1\u20133","key":"10.1016\/j.jconrel.2004.01.022_BIB15","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0169-409X(00)00133-2","article-title":"Key issues in non-viral gene delivery","volume":"46","author":"Pouton","year":"2001","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"2","key":"10.1016\/j.jconrel.2004.01.022_BIB16","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1106\/MVEK-KHWR-QUDG-CTYA","article-title":"Synthesis and evaluation of poly(ethylene glycol)-polylysine block copolymers as carriers for gene delivery","volume":"15","author":"Vanderkerken","year":"2000","journal-title":"J. Bioact. Compat. Polym."},{"issue":"4","key":"10.1016\/j.jconrel.2004.01.022_BIB17","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/BF00146856","article-title":"In-vitro evaluation of biocompatibility of different wound dressing materials","volume":"6","author":"Berscht","year":"1995","journal-title":"J. Mater. Sci. Mater. Med."},{"issue":"2","key":"10.1016\/j.jconrel.2004.01.022_BIB18","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0378-5173(96)04847-8","article-title":"Evaluation of the biological properties of soluble chitosan and chitosan microspheres","volume":"148","author":"Carreno Gomez","year":"1997","journal-title":"Int. J. Pharm."},{"issue":"9","key":"10.1016\/j.jconrel.2004.01.022_BIB19","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1021\/jm0105528","article-title":"Polysaccharide-oligoamine based conjugates for gene delivery","volume":"45","author":"Azzam","year":"2002","journal-title":"J. Med. Chem."},{"issue":"27","key":"10.1016\/j.jconrel.2004.01.022_BIB20","doi-asserted-by":"crossref","first-page":"9947","DOI":"10.1021\/ma0209592","article-title":"Cationic polysaccharides for gene delivery","volume":"35","author":"Azzam","year":"2002","journal-title":"Macromolecules"},{"key":"10.1016\/j.jconrel.2004.01.022_BIB21","series-title":"Dextran Prodrugs","author":"Larsen","year":"1990"},{"key":"10.1016\/j.jconrel.2004.01.022_BIB22","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/masy.200390130","article-title":"Dextran-spermine conjugate: an efficient vector for gene delivery","volume":"195","author":"Azzam","year":"2003","journal-title":"Macromol. Symp."},{"issue":"2","key":"10.1016\/j.jconrel.2004.01.022_BIB23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1615\/CritRevTherDrugCarrierSyst.v14.i2.20","article-title":"Nonviral vectors for in vivo gene delivery: physicochemical and pharmacokinetic considerations","volume":"14","author":"Mahato","year":"1997","journal-title":"Crit. Rev. Ther. Drug Carrier Syst."},{"issue":"2\u20133","key":"10.1016\/j.jconrel.2004.01.022_BIB24","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/0168-3659(95)00166-2","article-title":"Controllable gene therapy\u2014pharmaceutics of non-viral gene delivery systems","volume":"39","author":"Tomlinson","year":"1996","journal-title":"J. Control. Release"},{"issue":"1","key":"10.1016\/j.jconrel.2004.01.022_BIB25","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(98)00048-9","article-title":"Key issues in non-viral gene delivery","volume":"34","author":"Pouton","year":"1998","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"4","key":"10.1016\/j.jconrel.2004.01.022_BIB26","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/sj.gt.3300900","article-title":"PEGylated DNA\/transferrin\u2013PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery","volume":"6","author":"Ogris","year":"1999","journal-title":"Gene Ther."},{"issue":"4","key":"10.1016\/j.jconrel.2004.01.022_BIB27","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1006\/mthe.2002.0568","article-title":"Importance of lateral and steric stabilization of polyelectrolyte gene delivery vectors for extended systemic circulation","volume":"5","author":"Oupicky","year":"2002","journal-title":"Mol. Ther."},{"issue":"1","key":"10.1016\/j.jconrel.2004.01.022_BIB28","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1021\/bc0256087","article-title":"Novel shielded transferrin-polyethylene glycol-polyethylenimine\/DNA complexes for systemic tumor-targeted gene transfer","volume":"14","author":"Kursa","year":"2003","journal-title":"Bioconjugate Chem."},{"key":"10.1016\/j.jconrel.2004.01.022_BIB29","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/sj.gt.3302159","article-title":"Dextran-spermine polycation: an efficient non-viral vector for in vitro and in vivo gene transfection","volume":"11","author":"Hosseinkhani","year":"2004","journal-title":"Gene Ther."},{"issue":"2","key":"10.1016\/j.jconrel.2004.01.022_BIB30","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1002\/jps.10052","article-title":"Long-acting forms of sonic hedgehog with improved pharmacokinetic and pharmacodynamic properties are efficacious in a nerve injury model","volume":"91","author":"Pepinsky","year":"2002","journal-title":"J. Pharm. Sci."},{"issue":"10\u201312","key":"10.1016\/j.jconrel.2004.01.022_BIB31","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1002\/pat.245","article-title":"Molecular mass distribution of polycations and dextrans by high-performance size exclusion chromatography","volume":"13","author":"Kumar","year":"2002","journal-title":"Polym. Adv. Technol."},{"issue":"1","key":"10.1016\/j.jconrel.2004.01.022_BIB32","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/0003-2697(75)90431-5","article-title":"An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines","volume":"64","author":"Snyder","year":"1975","journal-title":"Anal. Biochem."},{"issue":"3","key":"10.1016\/j.jconrel.2004.01.022_BIB33","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/0092-8674(78)90254-4","article-title":"Biochemical transfer of single-copy eukaryotic genes using total cellular DNA as donor","volume":"14","author":"Wigler","year":"1978","journal-title":"Cell"},{"issue":"3","key":"10.1016\/j.jconrel.2004.01.022_BIB34","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1073\/pnas.76.3.1373","article-title":"DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian-cells","volume":"76","author":"Wigler","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"6","key":"10.1016\/j.jconrel.2004.01.022_BIB35","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1021\/bc990077g","article-title":"Fluorescent neoglycoproteins: antibody\u2013aminodextran\u2013phycobiliprotein conjugates","volume":"10","author":"Siiman","year":"1999","journal-title":"Bioconjugate Chem."},{"issue":"17","key":"10.1016\/j.jconrel.2004.01.022_BIB36","doi-asserted-by":"crossref","first-page":"8015","DOI":"10.1016\/S0021-9258(19)70595-X","article-title":"Monoclonal-antibody covalently coupled with fatty-acid\u2014a reagent for in vitro liposome targeting","volume":"255","author":"Huang","year":"1980","journal-title":"J. Biol. Chem."},{"issue":"20","key":"10.1016\/j.jconrel.2004.01.022_BIB37","doi-asserted-by":"crossref","first-page":"7859","DOI":"10.1021\/la000633r","article-title":"A new class of amphiphilic poly-l-lysine based polymers forms nanoparticles on probe sonication in aqueous media","volume":"16","author":"Wang","year":"2000","journal-title":"Langmuir"},{"issue":"2","key":"10.1016\/j.jconrel.2004.01.022_BIB38","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1006\/jcis.2001.7463","article-title":"The level of hydrophobic substitution and the molecular weight of amphiphilic poly-l-lysine-based polymers strongly affects their assembly into polymeric bilayer vesicles","volume":"237","author":"Wang","year":"2001","journal-title":"J. Colloid Interf. Sci."},{"issue":"1","key":"10.1016\/j.jconrel.2004.01.022_BIB39","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0168-3659(02)00412-1","article-title":"In vitro gene expression by cationized derivatives of an artificial protein with repeated RGD sequences, pronectin (R)","volume":"86","author":"Hosseinkhani","year":"2003","journal-title":"J. Control. Release"},{"issue":"5373","key":"10.1016\/j.jconrel.2004.01.022_BIB40","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1126\/science.281.5373.78","article-title":"An inverted hexagonal phase of cationic liposome\u2013DNA complexes related to DNA release and delivery","volume":"281","author":"Koltover","year":"1998","journal-title":"Science"},{"issue":"26","key":"10.1016\/j.jconrel.2004.01.022_BIB41","doi-asserted-by":"crossref","first-page":"14046","DOI":"10.1073\/pnas.97.26.14046","article-title":"DNA condensation in two dimensions","volume":"97","author":"Koltover","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"21","key":"10.1016\/j.jconrel.2004.01.022_BIB42","doi-asserted-by":"crossref","first-page":"7413","DOI":"10.1073\/pnas.84.21.7413","article-title":"Lipofection\u2014a highly efficient, lipid-mediated DNA\u2014transfection procedure","volume":"84","author":"Felgner","year":"1987","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"41","key":"10.1016\/j.jconrel.2004.01.022_BIB43","doi-asserted-by":"crossref","first-page":"13537","DOI":"10.1021\/bi00041a033","article-title":"A novel series of amphiphilic imidazolinium compounds for in-vitro and in-vivo gene delivery","volume":"34","author":"Solodin","year":"1995","journal-title":"Biochemistry"},{"issue":"7","key":"10.1016\/j.jconrel.2004.01.022_BIB44","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/nbt0797-647","article-title":"Improved DNA: liposome complexes for increased systemic delivery and gene expression","volume":"15","author":"Templeton","year":"1997","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"10.1016\/j.jconrel.2004.01.022_BIB45","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1021\/bc000089y","article-title":"Highly fluorinated lipospermines for gene transfer: synthesis and evaluation of their in vitro transfection efficiency","volume":"12","author":"Gaucheron","year":"2001","journal-title":"Bioconjugate Chem."}],"container-title":["Journal of Controlled Release"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168365904000434?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168365904000434?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T07:19:52Z","timestamp":1623741592000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168365904000434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,4]]}},"alternative-id":["S0168365904000434"],"URL":"https:\/\/doi.org\/10.1016\/j.jconrel.2004.01.022","relation":{},"ISSN":["0168-3659"],"issn-type":[{"value":"0168-3659","type":"print"}],"subject":[],"published":{"date-parts":[[2004,4]]}}}