{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:42:16Z","timestamp":1788910936113,"version":"build-2803163510"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T00:00:00Z","timestamp":1543536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Grand Challenges Explorations Initiative","award":["OPP1060394"],"award-info":[{"award-number":["OPP1060394"]}]},{"name":"Grand Challenges Explorations Initiative","award":["OPP1119024"],"award-info":[{"award-number":["OPP1119024"]}]},{"DOI":"10.13039\/501100008777","name":"Fundaci\u00f3n Bunge y Born","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008777","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Controlled Release"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.jconrel.2018.11.025","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T06:28:18Z","timestamp":1543300098000},"page":"158-171","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["Oral co-administration of a bacterial protease inhibitor in the vaccine formulation increases antigen delivery at the intestinal epithelial barrier"],"prefix":"10.1016","volume":"293","author":[{"given":"Lorena M.","family":"Coria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela S.","family":"Risso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco F.","family":"Guaimas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana C.","family":"Ferrero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bruno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina A.","family":"Pasquevich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7122-869X","authenticated-orcid":false,"given":"Juliana","family":"Cassataro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jconrel.2018.11.025_bb0005","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1600-065X.2010.00970.x","article-title":"Immunology of gut mucosal vaccines","volume":"239","author":"Pasetti","year":"2011","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0010","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1126\/science.1068284","article-title":"Outbreak of poliomyelitis in Hispaniola associated with circulating type 1 vaccine-derived poliovirus","volume":"296","author":"Kew","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0015","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/nri3251","article-title":"Recent progress in mucosal vaccine development: potential and limitations","volume":"12","author":"Lycke","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0020","series-title":"Oral Delivery of Macromolecular Drugs: Barriers, Strategies, and Future Trends","author":"Bernkop-Schn\u00fcrch","year":"2009"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0025","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1615\/CritRevTherDrugCarrierSyst.v20.i23.30","article-title":"Emerging trends in oral delivery of peptide and protein drugs","volume":"20","author":"Mahato","year":"2003","journal-title":"Crit. Rev. Ther. Drug Carrier Syst."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0030","series-title":"Physiology of the Gastrointestinal Tract","article-title":"Front-matter","author":"Leonard","year":"2012"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0035","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jconrel.2015.10.011","article-title":"A bacterial protease inhibitor protects antigens delivered in oral vaccines from digestion while triggering specific mucosal immune responses","volume":"220","author":"Ibanez","year":"2015","journal-title":"J. Control. Release"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0040","doi-asserted-by":"crossref","first-page":"4014","DOI":"10.4049\/jimmunol.1501188","article-title":"A Brucella spp. protease inhibitor limits antigen lysosomal proteolysis, increases cross-presentation, and enhances CD8+ T cell responses","volume":"196","author":"Coria","year":"2016","journal-title":"J. Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0045","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3389\/fimmu.2017.00171","article-title":"U-Omp19 from Brucella abortus is a useful adjuvant for vaccine formulations against Salmonella infection in mice","volume":"8","author":"Risso","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0016203","article-title":"An oral vaccine based on U-Omp19 induces protection against B. abortus mucosal challenge by inducing an adaptive IL-17 immune response in mice","volume":"6","author":"Pasquevich","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0055","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1128\/IAI.01151-08","article-title":"Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection","volume":"77","author":"Pasquevich","year":"2009","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0060","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/0005-2736(78)90440-6","article-title":"A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems","volume":"506","author":"Kessler","year":"1978","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0065","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev.cb.02.110186.001351","article-title":"Anchoring and biosynthesis of stalked brush border membrane proteins: glycosidases and peptidases of enterocytes and renal tubuli","volume":"2","author":"Semenza","year":"1986","journal-title":"Annu. Rev. Cell Biol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0070","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1136\/gut.30.12.1667","article-title":"Intestinal brush border revisited","volume":"30","author":"Holmes","year":"1989","journal-title":"Gut"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0075","doi-asserted-by":"crossref","first-page":"3098","DOI":"10.1002\/eji.201343740","article-title":"Dendritic cell subsets in the intestinal lamina propria: ontogeny and function","volume":"43","author":"Persson","year":"2013","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0080","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1038\/mi.2017.8","article-title":"Development of conventional dendritic cells: from common bone marrow progenitors to multiple subsets in peripheral tissues","volume":"10","author":"Sichien","year":"2017","journal-title":"Mucosal Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0085","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/mi.2017.22","article-title":"Diversity and functions of intestinal mononuclear phagocytes","volume":"10","author":"Joeris","year":"2017","journal-title":"Mucosal Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0090","doi-asserted-by":"crossref","first-page":"974","DOI":"10.3390\/toxins7030974","article-title":"Cholera toxin B: one subunit with many pharmaceutical applications","volume":"7","author":"Baldauf","year":"2015","journal-title":"Toxins"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0095","doi-asserted-by":"crossref","first-page":"4687","DOI":"10.1016\/j.vaccine.2010.05.008","article-title":"Cholera vaccines: WHO position paper-Recommendations","volume":"28","author":"Publication","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0100","first-page":"G558","article-title":"Horseradish peroxidase transport across adult rabbit jejunum in vitro","volume":"242","author":"Heyman","year":"1982","journal-title":"Am. J. Phys."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0105","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1002\/eji.200425882","article-title":"Immunomodulatory dendritic cells in intestinal lamina propria","volume":"35","author":"Chirdo","year":"2005","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0110","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1006\/smim.1999.0172","article-title":"M cells at locations outside the gut","volume":"11","author":"Gebert","year":"1999","journal-title":"Semin. Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0115","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.0818-9641.2004.01272.x","article-title":"Vaccine adjuvants: current state and future trends","volume":"82","author":"Petrovsky","year":"2004","journal-title":"Immunol. Cell Biol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0120","doi-asserted-by":"crossref","first-page":"736","DOI":"10.3109\/14756366.2015.1060483","article-title":"Interaction exists between matriptase inhibitors and intestinal epithelial cells","volume":"31","author":"Paszti-Gere","year":"2016","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0125","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1053\/j.gastro.2009.08.054","article-title":"HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cells","volume":"138","author":"Wu","year":"2010","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0130","doi-asserted-by":"crossref","first-page":"3097","DOI":"10.2174\/1381612821666150531163944","article-title":"Multifunctional delivery systems for advanced oral uptake of peptide\/protein drugs","volume":"21","author":"Park","year":"2015","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0135","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.addr.2016.01.010","article-title":"Oral delivery of macromolecular drugs: where we are after almost 100\u202fyears of attempts","volume":"101","author":"Moroz","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0140","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1248\/bpb.19.897","article-title":"Oral administration of insulin as poly(vinyl alcohol)-gel spheres in diabetic rats","volume":"19","author":"Kimura","year":"1996","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0145","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1023\/A:1018968611962","article-title":"Effects of various protease inhibitors on the intestinal absorption and degradation of insulin in rats","volume":"11","author":"Yamamoto","year":"1994","journal-title":"Pharm. Res."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0150","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1126\/science.1132742","article-title":"Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells","volume":"314","author":"Mora","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0155","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.1084\/jem.20091925","article-title":"Intestinal CD103+, but not CX3CR1+, antigen sampling cells migrate in lymph and serve classical dendritic cell functions","volume":"206","author":"Schulz","year":"2009","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0160","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.immuni.2004.08.011","article-title":"Retinoic acid imprints gut-homing specificity on T cells","volume":"21","author":"Iwata","year":"2004","journal-title":"Immunity"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0165","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/mi.2014.40","article-title":"Lymph-borne CD8alpha+ dendritic cells are uniquely able to cross-prime CD8+ T cells with antigen acquired from intestinal epithelial cells","volume":"8","author":"Cerovic","year":"2015","journal-title":"Mucosal Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0170","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/nri.2016.116","article-title":"Dendritic cell migration in health and disease","volume":"17","author":"Worbs","year":"2017","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0175","doi-asserted-by":"crossref","first-page":"15","DOI":"10.7774\/cevr.2017.6.1.15","article-title":"The development of mucosal vaccines for both mucosal and systemic immune induction and the roles played by adjuvants","volume":"6","author":"Kim","year":"2017","journal-title":"Clin. Exp. Vaccine Res."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0180","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0966-842X(98)01337-7","article-title":"Studying M cells and their role in infection","volume":"6","author":"Jepson","year":"1998","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0185","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1128\/IAI.67.2.946-953.1999","article-title":"Human intestinal M cells display the sialyl Lewis A antigen","volume":"67","author":"Giannasca","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0190","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1016\/j.vaccine.2004.11.010","article-title":"Mucosal adjuvants","volume":"23","author":"Freytag","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.jconrel.2018.11.025_bb0195","doi-asserted-by":"crossref","first-page":"8536","DOI":"10.1073\/pnas.161273098","article-title":"A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity","volume":"98","author":"Aman","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jconrel.2018.11.025_bb0200","doi-asserted-by":"crossref","first-page":"10094","DOI":"10.1073\/pnas.92.22.10094","article-title":"Transcytosis of cholera toxin subunits across model human intestinal epithelia","volume":"92","author":"Lencer","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."}],"container-title":["Journal of Controlled Release"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168365918306862?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168365918306862?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T02:02:53Z","timestamp":1605492173000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168365918306862"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":40,"alternative-id":["S0168365918306862"],"URL":"https:\/\/doi.org\/10.1016\/j.jconrel.2018.11.025","relation":{},"ISSN":["0168-3659"],"issn-type":[{"value":"0168-3659","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Oral co-administration of a bacterial protease inhibitor in the vaccine formulation increases antigen delivery at the intestinal epithelial barrier","name":"articletitle","label":"Article Title"},{"value":"Journal of Controlled Release","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jconrel.2018.11.025","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}