{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T02:16:47Z","timestamp":1793413007527,"version":"4.5.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["22-2102 Pj"],"award-info":[{"award-number":["22-2102 Pj"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007232","name":"Radiumhemmets Forskningsfonder","doi-asserted-by":"publisher","award":["181103"],"award-info":[{"award-number":["181103"]}],"id":[{"id":"10.13039\/501100007232","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Controlled Release"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.jconrel.2025.113665","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T12:14:58Z","timestamp":1742991298000},"page":"113665","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["A fusion protein that targets antigen-loaded extracellular vesicles to B cells enhances antigen-specific T cell expansion"],"prefix":"10.1016","volume":"382","author":[{"given":"Annemarijn","family":"Offens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loes","family":"Teeuwen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gozde","family":"Gucluler Akpinar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Steiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander","family":"Kooijmans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doste","family":"Mamand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Weissinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"K\u00e4ll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Eldh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar P.B.","family":"Wiklander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"El-Andaloussi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael C.I.","family":"Karlsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"Vader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Gabrielsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.jconrel.2025.113665_bb0005","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1158\/2326-6066.CIR-22-0540","article-title":"Antigen-loaded extracellular vesicles induce responsiveness to anti-PD-1 and anti-PD-L1 treatment in a checkpoint refractory melanoma model","volume":"11","author":"Veerman","year":"2023","journal-title":"Cancer Immunol. Res."},{"issue":"5","key":"10.1016\/j.jconrel.2025.113665_bb0010","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1038\/nm0598-594","article-title":"Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell-derived exosomes","volume":"4","author":"Zitvogel","year":"1998","journal-title":"Nat. Med."},{"issue":"4","key":"10.1016\/j.jconrel.2025.113665_bb0015","doi-asserted-by":"crossref","DOI":"10.1080\/2162402X.2015.1071008","article-title":"Dendritic cell-derived exosomes as maintenance immunotherapy after first line chemotherapy in NSCLC","volume":"5","author":"Besse","year":"2016","journal-title":"Oncoimmunology"},{"issue":"1","key":"10.1016\/j.jconrel.2025.113665_bb0020","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1479-5876-3-9","article-title":"A phase I study of dexosome immunotherapy in patients with advanced non-small cell lung cancer","volume":"3","author":"Morse","year":"2005","journal-title":"J. Transl. Med."},{"issue":"1","key":"10.1016\/j.jconrel.2025.113665_bb0025","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1479-5876-3-10","article-title":"Vaccination of metastatic melanoma patients with autologous dendritic cell (DC) derived-exosomes: results of thefirst phase I clinical trial","volume":"3","author":"Escudier","year":"2005","journal-title":"J. Transl. Med."},{"issue":"13","key":"10.1016\/j.jconrel.2025.113665_bb0030","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1158\/0008-5472.CAN-12-3918","article-title":"Synergistic induction of adaptive antitumor immunity by codelivery of antigen with \u03b1-galactosylceramide on exosomes","volume":"73","author":"Gehrmann","year":"2013","journal-title":"Cancer Res."},{"issue":"6","key":"10.1016\/j.jconrel.2025.113665_bb0035","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.4049\/jimmunol.1203082","article-title":"Dendritic cell-derived exosomes need to activate both T and B cells to induce antitumor immunity","volume":"190","author":"N\u00e4slund","year":"2013","journal-title":"J. Immunol."},{"issue":"7","key":"10.1016\/j.jconrel.2025.113665_bb0040","doi-asserted-by":"crossref","first-page":"4006","DOI":"10.4049\/jimmunol.0903009","article-title":"B cells are required for optimal CD4+ and CD8+ T cell tumor immunity: therapeutic B cell depletion enhances B16 melanoma growth in mice","volume":"184","author":"DiLillo","year":"2010","journal-title":"J. Immunol."},{"issue":"6","key":"10.1016\/j.jconrel.2025.113665_bb0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2020.02.015","article-title":"Complement signals determine opposite effects of B cells in chemotherapy-induced immunity","volume":"180","author":"Lu","year":"2020","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.jconrel.2025.113665_bb0050","doi-asserted-by":"crossref","first-page":"73","DOI":"10.4049\/jimmunol.1001145","article-title":"Exosomes containing glycoprotein 350 released by EBV-transformed B cells selectively target B cells through CD21 and block EBV infection in vitro","volume":"186","author":"Vallhov","year":"2011","journal-title":"J. Immunol."},{"issue":"5","key":"10.1016\/j.jconrel.2025.113665_bb0055","doi-asserted-by":"crossref","first-page":"2413","DOI":"10.1039\/C7NR06966A","article-title":"Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach","volume":"10","author":"Kooijmans","year":"2018","journal-title":"Nanoscale"},{"key":"10.1016\/j.jconrel.2025.113665_bb0060","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.omtm.2019.12.003","article-title":"Targeting antigen to the surface of EVs improves the in vivo immunogenicity of human and non-human adenoviral vaccines in mice","volume":"16","author":"Bliss","year":"2020","journal-title":"Mol. Ther. Methods Clin. Dev."},{"issue":"13","key":"10.1016\/j.jconrel.2025.113665_bb0065","doi-asserted-by":"crossref","first-page":"6507","DOI":"10.7150\/thno.56367","article-title":"Targeted delivery of neural progenitor cell-derived extracellular vesicles for anti-inflammation after cerebral ischemia","volume":"11","author":"Tian","year":"2021","journal-title":"Theranostics"},{"issue":"4","key":"10.1016\/j.jconrel.2025.113665_bb0070","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.4049\/jimmunol.177.4.2056","article-title":"Cutting edge: Epstein-Barr virus transactivates the HERV-K18 superantigen by docking to the human complement receptor 2 (CD21) on primary B cells","volume":"177","author":"Hsiao","year":"2006","journal-title":"J. Immunol."},{"issue":"6\u20137","key":"10.1016\/j.jconrel.2025.113665_bb0075","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.molimm.2010.12.019","article-title":"Human complement receptor type 2 (CR2\/CD21) transgenic mice provide an in vivo model to study immunoregulatory effects of receptor antagonists","volume":"48","author":"Kulik","year":"2011","journal-title":"Mol. Immunol."},{"issue":"12","key":"10.1016\/j.jconrel.2025.113665_bb0080","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1182\/blood-2008-04-153536","article-title":"Antigen-loaded exosomes alone induce Th1-type memory through a B-cell-dependent mechanism","volume":"113","author":"Qazi","year":"2009","journal-title":"Blood"},{"issue":"11","key":"10.1016\/j.jconrel.2025.113665_bb0085","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1038\/s41551-024-01214-6","article-title":"Antibody-displaying extracellular vesicles for targeted cancer therapy","volume":"8","author":"Wiklander","year":"2024","journal-title":"Nat. Biomed. Eng."},{"issue":"4","key":"10.1016\/j.jconrel.2025.113665_bb0090","doi-asserted-by":"crossref","first-page":"825","DOI":"10.4049\/jimmunol.1801628","article-title":"Allogenicity boosts extracellular vesicle-induced antigen-specific immunity and mediates tumor protection and long-term memory in vivo","volume":"203","author":"Larssen","year":"2019","journal-title":"J. Immunol."},{"issue":"1","key":"10.1016\/j.jconrel.2025.113665_bb0095","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-10646-x","article-title":"Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography","volume":"7","author":"Corso","year":"2017","journal-title":"Sci. Rep."},{"issue":"6","key":"10.1016\/j.jconrel.2025.113665_bb0100","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12238","article-title":"Identification of storage conditions stabilizing extracellular vesicles preparations","volume":"11","author":"G\u00f6rgens","year":"2022","journal-title":"J. Extracell Vesicles"},{"issue":"1","key":"10.1016\/j.jconrel.2025.113665_bb0105","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2020.1800222","article-title":"Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging","volume":"9","author":"Gupta","year":"2020","journal-title":"J. Extracellular Vesicles"},{"issue":"6","key":"10.1016\/j.jconrel.2025.113665_bb0110","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.4049\/jimmunol.172.6.3509","article-title":"Distinct sequences in the cytoplasmic domain of complement receptor 2 are involved in antigen internalization and presentation","volume":"172","author":"Barrault","year":"2004","journal-title":"J. Immunol."},{"issue":"8","key":"10.1016\/j.jconrel.2025.113665_bb0115","doi-asserted-by":"crossref","first-page":"2617","DOI":"10.1182\/blood.V95.8.2617","article-title":"Tracing uptake of C3dg-conjugated antigen into B cells via complement receptor type 2 (CR2, CD21)","volume":"95","author":"Hess","year":"2000","journal-title":"Blood"},{"issue":"9","key":"10.1016\/j.jconrel.2025.113665_bb0120","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1016\/j.molimm.2006.10.014","article-title":"Internalization and molecular interactions of human CD21 receptor","volume":"44","author":"Tessier","year":"2007","journal-title":"Mol. Immunol."},{"issue":"4","key":"10.1016\/j.jconrel.2025.113665_bb0125","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1002\/eji.1830170413","article-title":"Activation of human platelets through gp140, the C3d\/EBV receptor (CR2)","volume":"17","author":"Nunez","year":"1987","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.jconrel.2025.113665_bb0130","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1034\/j.1600-065X.2001.1800109.x","article-title":"CR1 and CR1-like: the primate immune adherence receptors","volume":"180","author":"Birmingham","year":"2001","journal-title":"Immunol. Rev."},{"issue":"12","key":"10.1016\/j.jconrel.2025.113665_bb0135","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1038\/nri2214","article-title":"Engulfment of apoptotic cells: signals for a good meal","volume":"7","author":"Ravichandran","year":"2007","journal-title":"Nat. Rev. Immunol."},{"issue":"25","key":"10.1016\/j.jconrel.2025.113665_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2021.11.007","article-title":"Neoantigen-driven B cell and CD4 T follicular helper cell collaboration promotes anti-tumor CD8 T cell responses","volume":"184","author":"Cui","year":"2021","journal-title":"Cell"},{"issue":"4","key":"10.1016\/j.jconrel.2025.113665_bb0145","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.immuni.2018.08.012","article-title":"B cells are the dominant antigen-presenting cells that activate naive CD4(+) T cells upon immunization with a virus-derived nanoparticle antigen","volume":"49","author":"Hong","year":"2018","journal-title":"Immunity"},{"issue":"5","key":"10.1016\/j.jconrel.2025.113665_bb0150","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1002\/eji.200737758","article-title":"Simultaneous presentation and cross-presentation of immune-stimulating complex-associated cognate antigen by antigen-specific B cells","volume":"38","author":"Robson","year":"2008","journal-title":"Eur. J. Immunol."},{"issue":"11","key":"10.1016\/j.jconrel.2025.113665_bb0155","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.2337\/db12-0006","article-title":"B-cell cross-presentation of autologous antigen precipitates diabetes","volume":"61","author":"Mari\u00f1o","year":"2012","journal-title":"Diabetes"},{"issue":"8","key":"10.1016\/j.jconrel.2025.113665_bb0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.109591","article-title":"B cells promote CD8 T cell primary and memory responses to subunit vaccines","volume":"36","author":"Klarquist","year":"2021","journal-title":"Cell Rep."},{"issue":"1","key":"10.1016\/j.jconrel.2025.113665_bb0165","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1038\/ni1542","article-title":"Follicular shuttling of marginal zone B cells facilitates antigen transport","volume":"9","author":"Cinamon","year":"2008","journal-title":"Nat. Immunol."},{"issue":"8","key":"10.1016\/j.jconrel.2025.113665_bb0170","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1038\/s41590-023-01559-1","article-title":"Long-term retention of antigens in germinal centers is controlled by the spatial organization of the follicular dendritic cell network","volume":"24","author":"Mart\u00ednez-Ria\u00f1o","year":"2023","journal-title":"Nat. Immunol."},{"issue":"10","key":"10.1016\/j.jconrel.2025.113665_bb0175","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1016\/j.immuni.2023.09.005","article-title":"CD8(+) T cells in the cancer-immunity cycle","volume":"56","author":"Giles","year":"2023","journal-title":"Immunity"},{"issue":"8","key":"10.1016\/j.jconrel.2025.113665_bb0180","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1038\/s41590-024-01896-9","article-title":"T cell dysfunction and therapeutic intervention in cancer","volume":"25","author":"Zebley","year":"2024","journal-title":"Nat. Immunol."},{"issue":"8","key":"10.1016\/j.jconrel.2025.113665_bb0185","first-page":"911","article-title":"Cancer vaccines: the next immunotherapy frontier","volume":"3","author":"Lin","year":"2022","journal-title":"Nat. Can."}],"container-title":["Journal of Controlled Release"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168365925002858?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168365925002858?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:00:34Z","timestamp":1777870834000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168365925002858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":37,"alternative-id":["S0168365925002858"],"URL":"https:\/\/doi.org\/10.1016\/j.jconrel.2025.113665","relation":{},"ISSN":["0168-3659"],"issn-type":[{"value":"0168-3659","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A fusion protein that targets antigen-loaded extracellular vesicles to B cells enhances antigen-specific T cell expansion","name":"articletitle","label":"Article Title"},{"value":"Journal of Controlled Release","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jconrel.2025.113665","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"113665"}}