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Nanobiotechnol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0375","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jconrel.2018.03.002","article-title":"Insights into maleimide-thiol conjugation chemistry: conditions for efficient surface functionalization of nanoparticles for receptor targeting","volume":"282","author":"Mart\u00ednez-Jothar","year":"2018","journal-title":"J. Control. Release"},{"key":"10.1016\/j.jconrel.2026.115363_bb0380","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1007\/s12274-023-6225-6","article-title":"Dynamic shielding of bacterial outer membrane vesicles for safe and efficient chemo-immunotherapy against tumors","volume":"17","author":"Li","year":"2024","journal-title":"Nano Res."},{"key":"10.1016\/j.jconrel.2026.115363_bb0385","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2403460121","article-title":"Bacterial outer membrane vesicle nanorobot","volume":"121","author":"Tang","year":"2024","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.jconrel.2026.115363_bb0390","doi-asserted-by":"crossref","first-page":"2455","DOI":"10.1021\/cr800323s","article-title":"Amino acid-protecting groups","volume":"109","author":"Isidro-Llobet","year":"2009","journal-title":"Chem. Rev."},{"key":"10.1016\/j.jconrel.2026.115363_bb0395","doi-asserted-by":"crossref","first-page":"3394","DOI":"10.1039\/c2cs35453e","article-title":"Biomolecule-functionalized polymer brushes","volume":"42","author":"Jiang","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.jconrel.2026.115363_bb0400","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2025.123725","article-title":"Engineered bacterial outer membrane vesicles enhanced tumor immunotherapy through remodeling tumor stroma and targeted delivery of CD73 siRNA","volume":"327","author":"Cheng","year":"2026","journal-title":"Biomaterials"},{"key":"10.1016\/j.jconrel.2026.115363_bb0405","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbagen.2020.129763","article-title":"A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles","volume":"1865","author":"Jiang","year":"2021","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.jconrel.2026.115363_bb0410","article-title":"Rapid surface display of mRNA antigens by bacteria-derived outer membrane vesicles for a personalized tumor vaccine","volume":"34","author":"Li","year":"2022","journal-title":"Adv. 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Med."},{"key":"10.1016\/j.jconrel.2026.115363_bb0435","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1111\/1751-7915.13572","article-title":"Glycine significantly enhances bacterial membrane vesicle production: a powerful approach for isolation of LPS-reduced membrane vesicles of probiotic Escherichia coli","volume":"13","author":"Hirayama","year":"2020","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0440","doi-asserted-by":"crossref","DOI":"10.1002\/biot.201800528","article-title":"Scalable production and isolation of extracellular vesicles: available sources and lessons from current industrial bioprocesses","volume":"14","author":"Paganini","year":"2019","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.jconrel.2026.115363_bb0445","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1093\/ibd\/izaf107","article-title":"Therapeutic potential and translational challenges for bacterial extracellular vesicles in inflammatory bowel disease","volume":"31","author":"Pirolli","year":"2025","journal-title":"Inflamm. Bowel Dis."},{"key":"10.1016\/j.jconrel.2026.115363_bb0455","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1038\/nmeth.2926","article-title":"Principles of genetic circuit design","volume":"11","author":"Brophy","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jconrel.2026.115363_bb0460","doi-asserted-by":"crossref","first-page":"1.5.1","DOI":"10.1002\/0471142727.mb0105s41","article-title":"Introduction to plasmid biology","volume":"41","author":"Feinbaum","year":"1998","journal-title":"Curr. Protoc. Mol. 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Biol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0480","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0003647","article-title":"A one pot, one step, precision cloning method with high throughput capability","volume":"3","author":"Engler","year":"2008","journal-title":"PLoS One"},{"key":"10.1016\/j.jconrel.2026.115363_bb0485","series-title":"Increased Cloning Efficiency by Cycle Restriction\u2212Ligation (CRL)","first-page":"35","volume":"2","author":"Pusch","year":"1997"},{"key":"10.1016\/j.jconrel.2026.115363_bb0490","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0022-2836(83)80284-8","article-title":"Studies on transformation of Escherichia coli with plasmids","volume":"166","author":"Hanahan","year":"1983","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0495","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1074\/jbc.M307628200","article-title":"Incorporation of heterologous outer membrane and periplasmic proteins into Escherichia coli outer membrane vesicles","volume":"279","author":"Kesty","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jconrel.2026.115363_bb0500","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jmb.2008.03.076","article-title":"Engineered bacterial outer membrane vesicles with enhanced functionality","volume":"380","author":"Kim","year":"2008","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0505","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.606629","article-title":"Conjugative DNA transfer from E. coli to transformation-resistant lactobacilli","volume":"12","author":"Samperio","year":"2021","journal-title":"Front. 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Biotechnol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0525","doi-asserted-by":"crossref","first-page":"583","DOI":"10.3390\/bioengineering10050583","article-title":"Different strategies affect enzyme packaging into bacterial outer membrane vesicles","volume":"10","author":"Dean","year":"2023","journal-title":"Bioengineering"},{"key":"10.1016\/j.jconrel.2026.115363_bb0530","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/nbt.2508","article-title":"RNA-guided editing of bacterial genomes using CRISPR-Cas systems","volume":"31","author":"Jiang","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0535","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1128\/jb.156.3.1292-1300.1983","article-title":"Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria","volume":"156","author":"Selvaraj","year":"1983","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0540","doi-asserted-by":"crossref","first-page":"5978","DOI":"10.1073\/pnas.100127597","article-title":"An efficient recombination system for chromosome engineering in Escherichia coli","volume":"97","author":"Yu","year":"2000","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.jconrel.2026.115363_bb0545","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.mimet.2009.10.018","article-title":"The ClosTron: mutagenesis in Clostridium refined and streamlined","volume":"80","author":"Heap","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.jconrel.2026.115363_bb0550","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.mimet.2007.05.021","article-title":"The ClosTron: a universal gene knock-out system for the genus Clostridium","volume":"70","author":"Heap","year":"2007","journal-title":"J. Microbiol. 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Biol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0570","doi-asserted-by":"crossref","first-page":"844","DOI":"10.3390\/microorganisms9040844","article-title":"Genome editing in bacteria: CRISPR-Cas and beyond","volume":"9","author":"Arroyo-Olarte","year":"2021","journal-title":"Microorganisms"},{"key":"10.1016\/j.jconrel.2026.115363_bb0575","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2026.1882312","article-title":"Advances in gene editing tools for four typical Gram-positive bacteria","volume":"17","author":"Liang","year":"2026","journal-title":"Front. 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Sci."},{"key":"10.1016\/j.jconrel.2026.115363_bb0600","doi-asserted-by":"crossref","DOI":"10.1080\/21505594.2024.2367783","article-title":"Outer membrane vesicles from genetically engineered Salmonella enterica serovar Typhimurium presenting Helicobacter pylori antigens UreB and CagA induce protection against Helicobacter pylori infection in mice","volume":"15","author":"Liu","year":"2024","journal-title":"Virulence"},{"key":"10.1016\/j.jconrel.2026.115363_bb0605","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1038\/nrmicro2814","article-title":"The twin-arginine translocation (Tat) protein export pathway","volume":"10","author":"Palmer","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0610","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.VMBF-0012-2015","article-title":"Bacterial secretion systems \u2013 an overview","volume":"4","author":"Green","year":"2016","journal-title":"Microbiol. 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Commun."},{"key":"10.1016\/j.jconrel.2026.115363_bb0625","doi-asserted-by":"crossref","first-page":"4223","DOI":"10.3390\/ijms26094223","article-title":"Hypervesiculation meets sec-targeting: enhancing heterologous protein loading in Salmonella Typhi outer membrane vesicles for delivery and immune response","volume":"26","author":"Fuentes","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jconrel.2026.115363_bb0630","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v3.24015","article-title":"Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen","volume":"3","author":"Fantappi\u00e8","year":"2014","journal-title":"J. Extracell. 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Release"},{"key":"10.1016\/j.jconrel.2026.115363_bb0650","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.resmic.2016.10.001","article-title":"Affinity purification of bacterial outer membrane vesicles (OMVs) utilizing a His-tag mutant","volume":"168","author":"Alves","year":"2017","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0655","doi-asserted-by":"crossref","first-page":"369","DOI":"10.5483\/BMBRep.2024-0056","article-title":"Structural stability for surface display of antigen 43 and application to bacterial outer membrane vesicles production","volume":"57","author":"Ahn","year":"2024","journal-title":"BMB Rep."},{"key":"10.1016\/j.jconrel.2026.115363_bb0645","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1128\/JB.06055-11","article-title":"Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ\/PhoP system in Escherichia coli","volume":"194","author":"Lippa","year":"2012","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0660","doi-asserted-by":"crossref","first-page":"35296","DOI":"10.1021\/acsnano.4c11412","article-title":"Utilizing engineered Bacteria as \u201ccell factories\u201d in vivo for intracellular RNA-loaded outer membrane vesicles\u2019 self-assembly in tumor treatment","volume":"18","author":"Sun","year":"2024","journal-title":"ACS Nano"},{"key":"10.1016\/j.jconrel.2026.115363_bb0665","doi-asserted-by":"crossref","DOI":"10.1002\/adma.70627","article-title":"Engineering CRISPR system-based bacterial outer membrane vesicle potentiates T cell immunity for enhanced cancer immunotherapy","volume":"37","author":"Wang","year":"2025","journal-title":"Adv. Mater."},{"key":"10.1016\/j.jconrel.2026.115363_bb0670","doi-asserted-by":"crossref","first-page":"8267","DOI":"10.1021\/acs.nanolett.5c01359","article-title":"Synthetic bacterial membrane vesicles as versatile antigen-display platforms against respiratory syncytial virus","volume":"25","author":"Meng","year":"2025","journal-title":"Nano Lett."},{"key":"10.1016\/j.jconrel.2026.115363_bb0675","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s12951-025-03191-7","article-title":"RBD-displaying OMV nanovaccine boosts immunity against SARS-CoV-2","volume":"23","author":"Feng","year":"2025","journal-title":"J. Nanobiotechnol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0680","first-page":"1","article-title":"Engineered outer membrane vesicles enhance solid tumour CAR-T cell therapy","author":"Li","year":"2026","journal-title":"Nat. Biomed. 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J. Mol. Sci."},{"key":"10.1016\/j.jconrel.2026.115363_bb0710","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3109\/1040841X.2013.804032","article-title":"Autotransporter-based cell surface display in Gram-negative bacteria","volume":"41","author":"Nicolay","year":"2015","journal-title":"Crit. Rev. Microbiol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0715","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.921272","article-title":"Phylogenetic classification and functional review of autotransporters","volume":"13","author":"Clarke","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jconrel.2026.115363_bb0720","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1128\/JB.182.13.3726-3733.2000","article-title":"Autodisplay: functional display of active \u03b2-lactamase on the surface of Escherichia coli by the AIDA-I autotransporter","volume":"182","author":"Lattemann","year":"2000","journal-title":"J. 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