{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:43:50Z","timestamp":1791308630891,"version":"4.3.1"},"reference-count":43,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2004,6]]},"DOI":"10.1016\/j.jcv.2003.10.002","type":"journal-article","created":{"date-parts":[[2003,12,11]],"date-time":"2003-12-11T11:49:25Z","timestamp":1071143365000},"page":"157-164","source":"Crossref","is-referenced-by-count":66,"title":["Assessment of automated DNA extraction coupled with real-time PCR for measuring Epstein\u2013Barr virus load in whole blood, peripheral mononuclear cells and plasma"],"prefix":"10.1016","volume":"30","author":[{"given":"Samira","family":"Fafi-Kremer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Brengel-Pesce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Bargu\u00e8s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Josette","family":"Bourgeat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odile","family":"Genoulaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Seigneurin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Morand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcv.2003.10.002_BIB1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1128\/JCM.38.2.613-619.2000","article-title":"High levels of Epstein\u2013Barr virus DNA in blood of solid-organ transplant recipients and their value in predicting posttransplant lymphoproliferative disorders","volume":"38","author":"Baldanti","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1006\/mcpr.1993.1014","article-title":"Rapid in situ hybridization for the diagnosis of latent Epstein\u2013Barr virus infection","volume":"7","author":"Barletta","year":"1993","journal-title":"Mol. Cell Probes"},{"key":"10.1016\/j.jcv.2003.10.002_BIB3","doi-asserted-by":"crossref","first-page":"4105","DOI":"10.1128\/JCM.40.11.4105-4113.2002","article-title":"Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented","volume":"40","author":"Boom","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB4","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1002\/jmv.2153","article-title":"Routine use of real-time quantitative PCR for laboratory diagnosis of Epstein\u2013Barr virus infections","volume":"66","author":"Brengel-Pesce","year":"2002","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB5","doi-asserted-by":"crossref","first-page":"4152","DOI":"10.1128\/JCM.39.11.4152-4154;2001","article-title":"Epstein\u2013Barr virus (EBV) DNA in sera of patients with primary EBV infection","volume":"39","author":"Chan","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB6","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1056\/NEJM200008173430707","article-title":"Epstein\u2013Barr virus infection","volume":"343","author":"Cohen","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jcv.2003.10.002_BIB7","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1002\/jmv.2071","article-title":"Quantification of Epstein\u2013Barr virus load in peripheral blood of human immunodeficiency virus-infected patients using real-time PCR","volume":"65","author":"Dehee","year":"2001","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB8","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/(SICI)1097-0215(19990820)84:4<442::AID-IJC20>3.0.CO;2-J","article-title":"Detection of Epstein\u2013Barr virus (EBV) genomes in the serum of patients with EBV-associated Hodgkin\u2019s disease","volume":"84","author":"Gallagher","year":"1999","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.jcv.2003.10.002_BIB9","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1093\/infdis\/170.2.436","article-title":"Detection of cell-free Epstein\u2013Barr virus DNA in serum during acute infectious mononucleosis","volume":"170","author":"Gan","year":"1994","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2003.10.002_BIB10","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1128\/JCM.40.2.351-358.2002","article-title":"Evaluation of use of Epstein\u2013Barr viral load in patients after allogeneic stem cell transplantation to diagnose and monitor post-transplant lymphoproliferative disease","volume":"40","author":"Gartner","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB11","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1182\/blood.V95.3.807.003k24_807_814","article-title":"Epstein\u2013Barr virus (EBV) load in bone marrow transplant recipients at risk to develop post-transplant lymphoproliferative disease: prophylactic infusion of EBV-specific cytotoxic T cells","volume":"95","author":"Gustafsson","year":"2000","journal-title":"Blood"},{"key":"10.1016\/j.jcv.2003.10.002_BIB12","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1046\/j.1365-2141.2001.03087.x","article-title":"Prospective monitoring of the Epstein\u2013Barr virus DNA by a real-time quantitative polymerase chain reaction after allogenic stem cell transplantation","volume":"115","author":"Hoshino","year":"2001","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB13","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1128\/JCM.39.2.564-569.2001","article-title":"Normalized quantification by real-time PCR of Epstein\u2013Barr virus load in patients at risk for post-transplant lymphoproliferative disorders","volume":"39","author":"Jabs","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB14","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S1525-1578(10)60446-1","article-title":"Development of real-time PCR assays for the quantitative detection of Epstein\u2013Barr virus and cytomegalovirus, comparison of TaqMan probes, and molecular beacons","volume":"5","author":"Jebbink","year":"2003","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.jcv.2003.10.002_BIB15","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1128\/JCM.37.1.132-136.1999","article-title":"Quantitative analysis of Epstein\u2013Barr virus load by using a real-time PCR assay","volume":"37","author":"Kimura","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB16","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1002\/jmv.1890460115","article-title":"Measurement by the polymerase chain reaction of the Epstein\u2013Barr virus load in infectious mononucleosis and AIDS-related non-Hodgkin\u2019s lymphomas","volume":"46","author":"Laroche","year":"1995","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0022-1759(02)00333-2","article-title":"Use of real-time PCR to measure Epstein\u2013Barr virus genomes in whole blood","volume":"270","author":"Leung","year":"2002","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jcv.2003.10.002_BIB18","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1128\/JCM.37.4.1113-1116.1999","article-title":"Detection of Epstein\u2013Barr virus DNA in sera from transplant recipients with lymphoproliferative disorders","volume":"37","author":"Limaye","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB19","first-page":"1188","article-title":"Quantitative analysis of cell-free Epstein\u2013Barr virus DNA in plasma of patients with nasopharyngeal carcinoma","volume":"59","author":"Lo","year":"1999","journal-title":"Cancer Res."},{"key":"10.1016\/j.jcv.2003.10.002_BIB20","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1086\/314691","article-title":"Persistently high Epstein\u2013Barr virus (EBV) loads in peripheral blood lymphocytes from patients with chronic active EBV infection","volume":"179","author":"Maeda","year":"1999","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2003.10.002_BIB21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1040-8428(02)00078-1","article-title":"Pathogenic roles for Epstein\u2013Barr virus (EBV) gene products in EBV-associated proliferative disorders","volume":"45","author":"Middeldorp","year":"2003","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB22","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1097\/00007890-200003150-00048","article-title":"Quantitation of Epstein\u2013Barr virus DNA in the blood of adult liver transplant recipients","volume":"69","author":"Mutimer","year":"2000","journal-title":"Transplantation"},{"key":"10.1016\/j.jcv.2003.10.002_BIB23","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1365-2613.2001.iep190.x","article-title":"Epstein\u2013Barr virus infection and human malignancies","volume":"82","author":"Niedobitek","year":"2001","journal-title":"Int. J. Exp. Pathol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB24","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1128\/JCM.38.2.712-715.2000","article-title":"Development of a real-time quantitative assay for detection of Epstein\u2013Barr virus","volume":"38","author":"Niesters","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB25","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1034\/j.1399-0012.2000.140406.x","article-title":"Usefulness of quantitative real-time polymerase chain reaction in following up patients with Epstein\u2013Barr virus infection after liver transplantation","volume":"14","author":"Orii","year":"2000","journal-title":"Clin. Transplant"},{"issue":"Suppl. 1","key":"10.1016\/j.jcv.2003.10.002_BIB26","doi-asserted-by":"crossref","first-page":"S38","DOI":"10.1086\/320903","article-title":"Diagnosis and management of post-transplant lymphoproliferative disorder in solid-organ transplant recipients","volume":"33","author":"Preiksaitis","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.jcv.2003.10.002_BIB27","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/0092-8674(86)90803-2","article-title":"The structure of the termini of the Epstein\u2013Barr virus as a marker of clonal cellular proliferation","volume":"47","author":"Raab-Traub","year":"1986","journal-title":"Cell"},{"key":"10.1016\/j.jcv.2003.10.002_BIB28","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0168-1702(01)00436-1","article-title":"Epstein\u2013Barr virus","volume":"82","author":"Rickinson","year":"2002","journal-title":"Virus Res."},{"key":"10.1016\/j.jcv.2003.10.002_BIB29","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1034\/j.1399-3062.2001.003002079.x","article-title":"Epstein\u2013Barr virus load monitoring: its role in the prevention and management of post-transplant lymphoproliferative disease","volume":"3","author":"Rowe","year":"2001","journal-title":"Transplant. Infect. Dis."},{"key":"10.1016\/j.jcv.2003.10.002_BIB30","first-page":"1046","article-title":"Epstein\u2013Barr virus DNA in serum\/plasma as a tumor marker for nasopharyngeal cancer","volume":"6","author":"Shotelersuk","year":"2000","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.jcv.2003.10.002_BIB31","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.1182\/blood.V98.13.3739","article-title":"Asymptomatic primary Epstein\u2013Barr virus infection occurs in the absence of blood T-cell repertoire perturbations despite high levels of systemic viral load","volume":"98","author":"Silins","year":"2001","journal-title":"Blood"},{"key":"10.1016\/j.jcv.2003.10.002_BIB32","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/sj.bmt.1703331","article-title":"A prospective study of Epstein\u2013Barr virus load in 85 hematopoietic stem cell transplants","volume":"29","author":"Sirvent-Von Bueltzingsloewen","year":"2002","journal-title":"Bone Marrow Transplant."},{"key":"10.1016\/j.jcv.2003.10.002_BIB33","doi-asserted-by":"crossref","first-page":"669","DOI":"10.3109\/10428190109060358","article-title":"Epstein\u2013Barr virus DNA quantitation assessed by a real-time polymerase chain reaction in a case of Burkitt\u2019s lymphoma","volume":"41","author":"Solassol","year":"2001","journal-title":"Leuk Lymphoma"},{"key":"10.1016\/j.jcv.2003.10.002_BIB34","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1128\/JCM.39.4.1211-1216.2001","article-title":"Towards standardization of Epstein\u2013Barr virus DNA load monitoring: unfractionated whole blood as preferred clinical specimen","volume":"39","author":"Stevens","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB35","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1182\/blood.V97.5.1165","article-title":"Frequent monitoring of Epstein\u2013Barr virus DNA load in unfractionated whole blood is essential for early detection of post-transplant lymphoproliferative disease in high-risk patients","volume":"97","author":"Stevens","year":"2001","journal-title":"Blood"},{"key":"10.1016\/j.jcv.2003.10.002_BIB36","doi-asserted-by":"crossref","first-page":"3986","DOI":"10.1128\/JCM.40.11.3986-3992.2002","article-title":"Comparison of quantitative competitive PCR with LightCycler-based PCR for measuring Epstein\u2013Barr virus DNA load in clinical specimens","volume":"40","author":"Stevens","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB37","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1086\/341460","article-title":"Absolute level of Epstein\u2013Barr virus DNA in human immunodeficiency virus type 1 infection is not predictive of AIDS-related non-Hodgkin lymphoma","volume":"186","author":"Van Baarle","year":"2002","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2003.10.002_BIB38","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1001\/archinte.162.7.837","article-title":"Epstein\u2013Barr viral load assessment in immunocompetent patients with fulminant infectious mononucleosis","volume":"162","author":"Van Laar","year":"2002","journal-title":"Arch. Intern. Med."},{"key":"10.1016\/j.jcv.2003.10.002_BIB39","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1055\/s-2000-9678","article-title":"Real-time polymerase chain reaction (RQ-PCR) for the monitoring of Epstein\u2013Barr virus (EBV) load in peripheral blood mononuclear cells","volume":"212","author":"Wagner","year":"2000","journal-title":"Klin. Padiatr."},{"key":"10.1016\/j.jcv.2003.10.002_BIB40","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1016\/S0959-8049(01)00152-6","article-title":"Detection of Epstein\u2013Barr virus DNA in peripheral blood of paediatric patients with Hodgkin\u2019s disease by real-time polymerase chain reaction","volume":"37","author":"Wagner","year":"2001","journal-title":"Eur. J. Cancer"},{"key":"10.1016\/j.jcv.2003.10.002_BIB41","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1097\/00007890-200109270-00006","article-title":"Patients at risk for development of post-transplant lymphoproliferative disorder: plasma versus peripheral blood mononuclear cells as material for quantification of Epstein\u2013Barr viral load by using real-time quantitative polymerase chain reaction","volume":"72","author":"Wagner","year":"2001","journal-title":"Transplantation"},{"key":"10.1016\/j.jcv.2003.10.002_BIB42","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1128\/JCM.33.7.1765-1768.1995","article-title":"Detection and quantification of virus DNA in plasma of patients with Epstein\u2013Barr virus-associated diseases","volume":"33","author":"Yamamoto","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2003.10.002_BIB43","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1099\/0022-1317-69-5-1051","article-title":"Epstein\u2013Barr virus gene expression in nasopharyngeal carcinoma","volume":"69","author":"Young","year":"1988","journal-title":"J. Gen. Virol."}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653203002828?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653203002828?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,3,28]],"date-time":"2020-03-28T00:16:04Z","timestamp":1585354564000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653203002828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["S1386653203002828"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2003.10.002","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}