{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:08:54Z","timestamp":1789427334524,"version":"build-2803163510"},"reference-count":37,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["journalofclinicalvirology.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1016\/j.jcv.2013.08.033","type":"journal-article","created":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T13:15:38Z","timestamp":1378991738000},"page":"528-534","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["Development and evaluation of NS1 specific monoclonal antibody based antigen capture ELISA and its implications in clinical diagnosis of West Nile virus infection"],"prefix":"10.1016","volume":"58","author":[{"given":"Divyasha","family":"Saxena","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti S.","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manmohan","family":"Parida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rathinam R.","family":"Sivakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishan K.","family":"Patro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Pt 1","key":"10.1016\/j.jcv.2013.08.033_bib0005","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1099\/0022-1317-70-1-37","article-title":"Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera","volume":"70","author":"Calisher","year":"1989","journal-title":"J Gen Virol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0010","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1126\/science.286.5448.2333","article-title":"Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States","volume":"286","author":"Lanciotti","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.jcv.2013.08.033_bib0015","doi-asserted-by":"crossref","first-page":"306","DOI":"10.3201\/eid1102.040817","article-title":"West Nile virus in Morocco","volume":"11","author":"Schuffenecker","year":"2003","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.jcv.2013.08.033_bib0020","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1001\/jama.290.4.511","article-title":"Neurologic manifestations and outcome of West Nile virus infection","volume":"290","author":"Sejvar","year":"2003","journal-title":"JAMA"},{"key":"10.1016\/j.jcv.2013.08.033_bib0025","doi-asserted-by":"crossref","first-page":"788","DOI":"10.3201\/eid0907.030129","article-title":"Acute flaccid paralysis and West Nile virus infection","volume":"9","author":"Sejvar","year":"2003","journal-title":"Emerg Infect Dis"},{"issue":"Pt 3","key":"10.1016\/j.jcv.2013.08.033_bib0030","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1099\/0022-1317-66-3-559","article-title":"Synthesis of proteins and glycoproteins in dengue type 2 virus-infected vero and Aedes albopictus cells","volume":"66","author":"Smith","year":"1985","journal-title":"J Gen Virol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0035","doi-asserted-by":"crossref","first-page":"13924","DOI":"10.1128\/JVI.79.22.13924-13933.2005","article-title":"NS1 protein secretion during the acute phase of West Nile virus infection","volume":"79","author":"Macdonald","year":"2005","journal-title":"J Virol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0040","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/0042-6822(89)90544-8","article-title":"Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane associated after dimerization","volume":"171","author":"Winkler","year":"1989","journal-title":"Virology"},{"key":"10.1016\/j.jcv.2013.08.033_bib0045","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1128\/JCM.40.02.376-381.2002","article-title":"Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections","volume":"40","author":"Alcon","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0050","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.jcv.2013.08.033_bib0055","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/978-1-4613-0641-2_6","article-title":"Purification of recombinant protein with metal chelate adsorbent","volume":"12","author":"Hochuli","year":"1990","journal-title":"Genet Eng (NY)"},{"key":"10.1016\/j.jcv.2013.08.033_bib0060","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/0003-2697(89)90101-2","article-title":"Protein measurement using bicinchoninic acid: elimination of interfering substances","volume":"180","author":"Brown","year":"1989","journal-title":"Anal Biochem"},{"key":"10.1016\/j.jcv.2013.08.033_bib0065","first-page":"119","article-title":"Enzyme linked immunosorbent assay (ELISA) for antibody titers against chikungunya virus on human sera from Kenya","volume":"25","author":"Nakitare","year":"1983","journal-title":"Trop Med"},{"key":"10.1016\/j.jcv.2013.08.033_bib0070","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/256495a0","article-title":"Continuous cultures of fused cells secreting antibody of predefined specificity","volume":"256","author":"Kohler","year":"1975","journal-title":"Nature"},{"key":"10.1016\/j.jcv.2013.08.033_bib0075","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/0003-2697(83)90035-0","article-title":"A simple and rapid dot-immunobinding assay for zein and other prolamins","volume":"132","author":"Esen","year":"1983","journal-title":"Anal Biochem"},{"key":"10.1016\/j.jcv.2013.08.033_bib0080","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jcv.2013.08.033_bib0085","doi-asserted-by":"crossref","DOI":"10.1002\/0471143030.cb0403s00","article-title":"Immunofluorescence staining","volume":"4","author":"Donaldson","year":"2001","journal-title":"Curr Protoc Cell Biol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0090","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0166-0934(85)90139-9","article-title":"Detection of chikungunya virus antigen in Aedes albopictus mosquitoes by enzyme-linked immunosorbent assay","volume":"12","author":"Konishi","year":"1985","journal-title":"J Virol Methods"},{"key":"10.1016\/j.jcv.2013.08.033_bib0095","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/0019-2791(71)90454-X","article-title":"Enzyme linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G","volume":"8","author":"Engvall","year":"1971","journal-title":"Immunochemistry"},{"issue":"3","key":"10.1016\/j.jcv.2013.08.033_bib0100","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1743-422X-3-55","article-title":"Reemergence of Dengue Virus Type-3 (Subtype-III) in India: implications for increased incidences of DHF and DSS","volume":"6","author":"Dash","year":"2006","journal-title":"Virol J"},{"key":"10.1016\/j.jcv.2013.08.033_bib0105","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1089\/vbz.2006.0648","article-title":"East Central South African genotype as the causative agent in reemergence of chikungunya outbreak in India","volume":"4","author":"Dash","year":"2007","journal-title":"Vector Borne Zoonotic Dis"},{"key":"10.1016\/j.jcv.2013.08.033_bib0110","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.3201\/eid1209.060200","article-title":"Japanese Encephalitis outbreak, India, 2005","volume":"12","author":"Parida","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.jcv.2013.08.033_bib0115","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jcv.2007.04.015","article-title":"Development and evaluation of SYBR Green I-based one-step real-time RTPCR assay for detection and quantification of chikungunya virus","volume":"39","author":"Santhosh","year":"2007","journal-title":"J Clin Virol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0120","doi-asserted-by":"crossref","first-page":"4066","DOI":"10.1128\/JCM.38.11.4066-4071.2000","article-title":"Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay","volume":"38","author":"Lanciotti","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.jcv.2013.08.033_bib0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijid.2011.09.020","article-title":"Molecular detection and characterization of West Nile virus associated with multifocal retinitis in patients from southern India","volume":"16","author":"Shukla","year":"2012","journal-title":"Int J Infect Dis"},{"issue":"4","key":"10.1016\/j.jcv.2013.08.033_bib0130","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.diagmicrobio.2012.12.007","article-title":"Cloning and expression of an envelope gene of West Nile virus and evaluation of the protein for use in an IgM ELISA","volume":"75","author":"Saxena","year":"2013","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"1","key":"10.1016\/j.jcv.2013.08.033_bib0135","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3109\/08923973.2012.736521","article-title":"West Nile virus diagnosis and vaccination: using unique viral peptide sequences to evoke specific immune responses","volume":"35","author":"Capone","year":"2013","journal-title":"Immunopharmacol Immunotoxicol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0140","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/1743-422X-9-81","article-title":"West Nile virus: characterization and diagnostic applications of monoclonal antibodies","volume":"9","author":"Lelli","year":"2012","journal-title":"Virol J"},{"issue":"1","key":"10.1016\/j.jcv.2013.08.033_bib0145","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.tvjl.2012.08.019","article-title":"Review of diagnostic plaque reduction neutralization tests for flavivirus infection","volume":"195","author":"Maeda","year":"2013","journal-title":"Vet J"},{"key":"10.1016\/j.jcv.2013.08.033_bib0150","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1128\/JCM.38.3.1053-1057.2000","article-title":"An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients","volume":"38","author":"Young","year":"2000","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jcv.2013.08.033_bib0155","doi-asserted-by":"crossref","first-page":"5087","DOI":"10.1128\/JCM.42.11.5087-5093.2004","article-title":"Development and evaluation of an enzyme-linked immunosorbent assay for quantifying antibodies to Japanese encephalitis virus nonstructural 1 protein to detect subclinical infections in vaccinated horses","volume":"42","author":"Konishi","year":"2004","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.jcv.2013.08.033_bib0160","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1177\/104063870601800112","article-title":"Evaluation of antigen-capture ELISA and immunohistochemical methods for avian surveillance of West Nile virus","volume":"18","author":"Godhardt","year":"2006","journal-title":"J Vet Diagn Invest"},{"issue":"6","key":"10.1016\/j.jcv.2013.08.033_bib0165","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1128\/JCM.40.6.2023-2030.2002","article-title":"Detection of West Nile virus antigen in mosquitoes and avian tissues by a monoclonal antibody-based capture enzyme immunoassay","volume":"40","author":"Hunt","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"2","key":"10.1016\/j.jcv.2013.08.033_bib0170","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1128\/CVI.05382-11","article-title":"New sensitive competitive enzyme-linked immunosorbent assay using a monoclonal antibody against nonstructural protein 1 of West Nile virus NY99","volume":"19","author":"Hirota","year":"2012","journal-title":"Clin Vaccine Immunol"},{"issue":"1\u20132","key":"10.1016\/j.jcv.2013.08.033_bib0175","first-page":"23","article-title":"Homology among eleven flavivirus by comparative nucleotide sequence of geomic RNAs and deduced amino acid sequences of viral proteins","volume":"33","author":"Tanaka","year":"1991","journal-title":"Trop Med"},{"key":"10.1016\/j.jcv.2013.08.033_bib0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.ophtha.2013.02.006","article-title":"Molecular diagnosis and ocular imaging of West Nile virus retinitis and neuroretinitis","author":"Sivakumar","year":"2013","journal-title":"Ophthalmology"},{"key":"10.1016\/j.jcv.2013.08.033_bib0185","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1006\/viro.2002.1449","article-title":"Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe, and the Middle East","volume":"298","author":"Lanciotti","year":"2002","journal-title":"Virology"}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653213003570?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653213003570?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T22:54:33Z","timestamp":1596495273000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653213003570"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["S1386653213003570"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2013.08.033","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and evaluation of NS1 specific monoclonal antibody based antigen capture ELISA and its implications in clinical diagnosis of West Nile virus infection","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2013.08.033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}