{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T01:14:13Z","timestamp":1780535653955,"version":"3.54.1"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["journalofclinicalvirology.com","clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2015,8]]},"DOI":"10.1016\/j.jcv.2015.06.090","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T12:53:14Z","timestamp":1434718394000},"page":"150-155","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Identification of newly detected, drug-related HCMV UL97- and UL54-mutations using a modified plaque reduction assay"],"prefix":"10.1016","volume":"69","author":[{"given":"Lena","family":"Fischer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin Laib","family":"Sampaio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Jahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Hamprecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"G\u00f6hring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcv.2015.06.090_bib0005","doi-asserted-by":"crossref","first-page":"847","DOI":"10.3851\/IMP1299","article-title":"Genetic analysis and putative role in resistance to antivirals of the human cytomegalovirus DNA polymerase UL44 processivity factor","volume":"14","author":"Boutolleau","year":"2009","journal-title":"Antivir. Ther."},{"key":"10.1016\/j.jcv.2015.06.090_bib0010","doi-asserted-by":"crossref","first-page":"3752","DOI":"10.1128\/AAC.00165-09","article-title":"Fluorescence-based assay for phenotypic characterization of human cytomegalovirus polymerase mutations regarding drug susceptibility and viral replicative fitness","volume":"53","author":"Chevillotte","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jcv.2015.06.090_bib0015","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1093\/infdis\/171.3.576","article-title":"Analysis of the UL97 phosphotransferase coding sequence in clinical cytomegalovirus isolates and identification of mutations conferring ganciclovir resistance","volume":"171","author":"Chou","year":"1995","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2015.06.090_bib0020","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1093\/infdis\/172.1.239","article-title":"Frequency of UL97 phosphotransferase mutations related to ganciclovir resistance in clinical cytomegalovirus isolates","volume":"172","author":"Chou","year":"1995","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2015.06.090_bib0025","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1086\/517302","article-title":"Evolution of mutations conferring multidrug resistance during prophylaxis and therapy for cytomegalovirus disease","volume":"176","author":"Chou","year":"1997","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2015.06.090_bib0030","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1128\/AAC.43.6.1500","article-title":"Interstrain variation in the human cytomegalovirus DNA polymerase sequence and its effect on genotypic diagnosis of antiviral drug resistance Adult AIDS Clinical Trials Group CMV Laboratories","volume":"43","author":"Chou","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jcv.2015.06.090_bib0035","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1086\/338362","article-title":"Cytomegalovirus UL97 phosphotransferase mutations that affect susceptibility to ganciclovir","volume":"185","author":"Chou","year":"2002","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2015.06.090_bib0040","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1086\/375743","article-title":"Viral DNA polymerase mutations associated with drug resistance in human cytomegalovirus","volume":"188","author":"Chou","year":"2003","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2015.06.090_bib0045","doi-asserted-by":"crossref","first-page":"2710","DOI":"10.1128\/AAC.49.7.2710-2715.2005","article-title":"Phenotyping of cytomegalovirus drug resistance muations by using recombinant virus incorporating a reporter gene","volume":"49","author":"Chou","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jcv.2015.06.090_bib0050","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jcv.2008.04.005","article-title":"Contrasting drug resistance phenotypes resulting from cytomegalovirus DNA polymerase mutations at the same exonuclease locus","volume":"43","author":"Chou","year":"2008","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2015.06.090_bib0055","doi-asserted-by":"crossref","first-page":"5927","DOI":"10.1128\/JVI.72.7.5927-5936.1998","article-title":"Characterization of drug resistance-associated mutations in the human cytomegalovirus DNA polymerase gene by using recombinant mutant viruses generated from overlapping DNA fragments","volume":"72","author":"Cihlar","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.jcv.2015.06.090_bib0060","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1128\/JCM.01275-13","article-title":"Novel method based on \u201cen passant\u201d mutagenesis coupled with a gaussia luciferase reporter assay for studying the combined effects of human cytomegalovirus mutations","volume":"51","author":"Drouot","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2015.06.090_bib0065","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.1182\/blood.V96.9.3286","article-title":"Drug-resistant human cytomegalovirus infection in children after allogeneic stem cell transplantation may have different clinical outcomes","volume":"96","author":"Eckle","year":"2000","journal-title":"Blood"},{"key":"10.1016\/j.jcv.2015.06.090_bib0070","doi-asserted-by":"crossref","first-page":"8039","DOI":"10.1073\/pnas.140123497","article-title":"Prediction of cytomegalovirus load and resistance patterns after antiviral chemotherapy","volume":"97","author":"Emery","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jcv.2015.06.090_bib0075","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/CMR.12.2.286","article-title":"Resistance of human cytomegalovirus to antiviral drugs","volume":"12","author":"Erice","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.jcv.2015.06.090_bib0080","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.antiviral.2013.09.026","article-title":"Generation and characterization of a GCV resistant HCMV UL97-mutation and a drug sensitive UL54-mutation","volume":"100","author":"Fischer","year":"2013","journal-title":"Antiviral Res."},{"key":"10.1016\/j.jcv.2015.06.090_bib0085","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jcv.2013.01.003","article-title":"Dynamics of coexisting HCMV-UL97 and UL54 drug-resistance associated mutations in patients after haematopoietic cell transplantation","volume":"57","author":"G\u00f6hring","year":"2013","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2015.06.090_bib0090","first-page":"1064","article-title":"Virus-host interactions: methods and protocols","author":"Laib Sampaio","year":"2013","journal-title":"Methods Mol. Bio."},{"key":"10.1016\/j.jcv.2015.06.090_bib0095","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1086\/315851","article-title":"Prevalence of antiviral drug resistance in untreated patients with cytomegalovirus retinitis","volume":"182","author":"Liu","year":"2000","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2015.06.090_bib0100","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.1128\/AAC.45.10.2775-2780.2001","article-title":"Sequencing of cytomegalovirus UL97 gene for genotypic antiviral resistance testing","volume":"45","author":"Lurain","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jcv.2015.06.090_bib0105","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1128\/CMR.00009-10","article-title":"Antiviral drug resistance of human cytomegalovirus","volume":"23","author":"Lurain","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.jcv.2015.06.090_bib0110","series-title":"Virology Methods Manual","author":"Mahy","year":"1996"},{"key":"10.1016\/j.jcv.2015.06.090_bib0115","doi-asserted-by":"crossref","first-page":"579","DOI":"10.3851\/IMP1565","article-title":"Opposite effects of two cytomegalovirus DNA polymerase mutations on replicative capacity and polymerase activity","volume":"15","author":"Martin","year":"2010","journal-title":"Antivir. Ther."},{"key":"10.1016\/j.jcv.2015.06.090_bib0120","doi-asserted-by":"crossref","first-page":"5663","DOI":"10.1128\/JVI.73.7.5663-5670.1999","article-title":"A recombinant human cytomegalovirus with a large deletion in UL97 has a severe replication deficiency","volume":"73","author":"Pichard","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.jcv.2015.06.090_bib0125","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0928-0197(98)00043-9","article-title":"A simplified assay for screening of drug resistance of cell-associated cytomegalovirus strains","volume":"11","author":"Prix","year":"1998","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2015.06.090_bib0130","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1128\/AAC.00633-06","article-title":"Multidrug resistance conferred by novel DNA polymerase mutations in human cytomegalovirus isolates","volume":"51","author":"Scott","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jcv.2015.06.090_bib0135","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1002\/prot.21005","article-title":"Three-dimensional modelling of cytomegalovirus DNA polymerase and preliminary analysis of drug resistance","volume":"64","author":"Shi","year":"2006","journal-title":"Proteins"},{"key":"10.1016\/j.jcv.2015.06.090_bib0140","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jcv.2007.12.002","article-title":"Entry route of HCMV into endothelial cells","volume":"41","author":"Sinzger","year":"2007","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2015.06.090_bib0145","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1086\/514041","article-title":"High-level resistance of cytomegalovirus to ganciclovir is associated with alterations in both the UL97 and DNA polymerase genes","volume":"176","author":"Smith","year":"1997","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2015.06.090_bib0150","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2144\/000112096","article-title":"Two-step mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli","volume":"40","author":"Tischer","year":"2006","journal-title":"Biotechniques"},{"key":"10.1016\/j.jcv.2015.06.090_bib0155","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/978-1-60761-652-8_30","article-title":"En passant mutagenesis: a two stepp markerless recombination system","volume":"634","author":"Tischer","year":"2010","journal-title":"Methods Mol. Bio"},{"issue":"50","key":"10.1016\/j.jcv.2015.06.090_bib0160","doi-asserted-by":"crossref","first-page":"20037","DOI":"10.1073\/pnas.0709704104","article-title":"Human cytomegalovirus uses two distinct pathways to enter retinal pigmented epithelial cells","volume":"104","author":"Wang","year":"2007","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.jcv.2015.06.090_bib0165","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1172\/JCI117648","article-title":"Mutations in human cytomegalovirus UL97 gene confer clinical resistance to ganciclovir and can be detected directly in patient plasma","volume":"95","author":"Wolf","year":"1995","journal-title":"J. Clin. Invest."}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653215002759?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653215002759?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T17:53:26Z","timestamp":1599155606000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653215002759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":33,"alternative-id":["S1386653215002759"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2015.06.090","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2015,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of newly detected, drug-related HCMV UL97- and UL54-mutations using a modified plaque reduction assay","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2015.06.090","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}