{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:24:01Z","timestamp":1791289441651,"version":"4.3.0"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["journalofclinicalvirology.com","clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1016\/j.jcv.2016.07.008","type":"journal-article","created":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T13:52:05Z","timestamp":1468936325000},"page":"70-75","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Utility of droplet digital PCR for the quantitative detection of polyomavirus JC in clinical samples"],"prefix":"10.1016","volume":"82","author":[{"given":"Irene","family":"Giovannelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunziata","family":"Ciccone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guendalina","family":"Vaggelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunzia Della","family":"Malva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Torricelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Giannecchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcv.2016.07.008_bib0005","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1128\/CMR.05031-11","article-title":"Molecular biology, epidemiology, and pathogenesis of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain","volume":"25","author":"Ferenczy","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.jcv.2016.07.008_bib0010","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1056\/NEJMoa1107829","article-title":"Risk of natalizumab-associated progressive multifocal leukoencephalopathy","volume":"366","author":"Bloomgren","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jcv.2016.07.008_bib0015","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1056\/NEJMoa0904267","article-title":"Asymptomatic reactivation of JC virus in patients treated with natalizumab","volume":"361","author":"Chen","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jcv.2016.07.008_bib0020","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/ana.22128","article-title":"Anti-JC virus antibodies: implications for PML risk stratification","volume":"68","author":"Gorelik","year":"2010","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0025","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S1474-4422(10)70040-5","article-title":"Progressive multifocal leukoencephalopathy and other disorders caused by JC virus: clinical features and pathogenesis","volume":"9","author":"Tan","year":"2010","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0030","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1002\/ana.22107","article-title":"Assessment of JC virus DNA in blood and urine from natalizumab-treated patients","volume":"68","author":"Rudick","year":"2010","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0035","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1007\/s00415-009-5444-4","article-title":"JCV detection in multiple sclerosis patients treated with natalizumab","volume":"257","author":"Sadiq","year":"2010","journal-title":"J. Neurol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0040","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1212\/WNL.0b013e31828c2fa1","article-title":"PML diagnostic criteria: consensus statement from the AAN Neuroinfectious Disease Section","volume":"80","author":"Berger","year":"2013","journal-title":"Neurology"},{"key":"10.1016\/j.jcv.2016.07.008_bib0045","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jcv.2013.03.009","article-title":"Multiplex qPCR assay for ultra sensitive detection of JCV DNA with simultaneous identification of genotypes that discriminates non-virulent from virulent variants","volume":"57","author":"Ryschkewitsch","year":"2013","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.diagmicrobio.2012.10.009","article-title":"Viral diagnostics in the era of digital polymerase chain reaction","volume":"75","author":"Sedlak","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jcv.2016.07.008_bib0055","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1016\/j.clinbiochem.2015.03.015","article-title":"Digital PCR analysis of circulating nucleic acids","volume":"48","author":"Hudecova","year":"2015","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.jcv.2016.07.008_bib0060","doi-asserted-by":"crossref","first-page":"8604","DOI":"10.1021\/ac202028g","article-title":"High-throughput droplet digital PCR system for absolute quantitation of DNA copy number","volume":"83","author":"Hindson","year":"2011","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jcv.2016.07.008_bib0065","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1021\/ac202578x","article-title":"Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification","volume":"84","author":"Pinheiro","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jcv.2016.07.008_bib0070","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/ana.22137","article-title":"JC virus persistence following progressive multifocal leukoencephalopathy in multiple sclerosis patients treated with natalizumab","volume":"68","author":"Ryschkewitsch","year":"2010","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0075","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1128\/JVI.02565-14","article-title":"JC virus quasispecies analysis reveals a complex viral population underlying progressive multifocal leukoencephalopathy and supports viral dissemination via the hematogenous route","volume":"89","author":"Van Loy","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0080","doi-asserted-by":"crossref","first-page":"e82","DOI":"10.1093\/nar\/gks203","article-title":"Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation","volume":"40","author":"Whale","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jcv.2016.07.008_bib0085","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/1471-2164-10-116","article-title":"Digital PCR provides sensitive and absolute calibration for high throughput sequencing","volume":"10","author":"White","year":"2009","journal-title":"BMC Genom."},{"key":"10.1016\/j.jcv.2016.07.008_bib0090","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1126\/science.1200758","article-title":"Probing individual environmental bacteria for viruses by using microfluidic digital PCR","volume":"333","author":"Tadmor","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.jcv.2016.07.008_bib0095","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1128\/JCM.06800-11","article-title":"Clinical significance of low cytomegalovirus DNA levels in human plasma","volume":"50","author":"Waggoner","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2016.07.008_bib0100","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jviromet.2011.09.017","article-title":"Digital PCR provides absolute quantitation of viral load for an occult RNA virus","volume":"179","author":"White","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2016.07.008_bib0105","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1039\/c1lc20128j","article-title":"Quantitative and sensitive detection of rare mutations using droplet-based microfluidics","volume":"11","author":"Pekin","year":"2011","journal-title":"Lab Chip"}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653216301780?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653216301780?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T07:25:12Z","timestamp":1536564312000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653216301780"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":21,"alternative-id":["S1386653216301780"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2016.07.008","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Utility of droplet digital PCR for the quantitative detection of polyomavirus JC in clinical samples","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2016.07.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}