{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T16:06:17Z","timestamp":1793462777851,"version":"4.5.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.jcv.2020.104513","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T11:59:43Z","timestamp":1592308783000},"page":"104513","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"special_numbering":"C","title":["Assessing the burden of viral co-infections in acute gastroenteritis in children: An eleven-year-long investigation"],"prefix":"10.1016","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7748-0880","authenticated-orcid":false,"given":"Simona","family":"De Grazia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Floriana","family":"Bonura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celestino","family":"Bonura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Mangiaracina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Filizzolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5740-6947","authenticated-orcid":false,"given":"Vito","family":"Martella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni M.","family":"Giammanco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcv.2020.104513_bib0005","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1473-3099(19)30401-3","article-title":"Quantifying risks and interventions that have affected the burden of diarrhoea among children younger than 5 years: an analysis of the Global Burden of Disease Study 2017","volume":"20","author":"Troeger","year":"2020","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jcv.2020.104513_bib0010","doi-asserted-by":"crossref","DOI":"10.3390\/v8020042","article-title":"Viruses causing gastroenteritis: the known, the new and those beyond","volume":"8","author":"Oude Munnink","year":"2016","journal-title":"Viruses"},{"key":"10.1016\/j.jcv.2020.104513_bib0015","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s40249-016-0157-2","article-title":"Impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest China","volume":"5","author":"Zhang","year":"2016","journal-title":"Infect. Dis. Poverty"},{"key":"10.1016\/j.jcv.2020.104513_bib0020","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1099\/jgv.0.001318","article-title":"Updated classification of norovirus genogroups and genotypes","volume":"100","author":"Chhabra","year":"2019","journal-title":"J. Gen Virol."},{"key":"10.1016\/j.jcv.2020.104513_bib0025","series-title":"Astroviruses Pp609-650","author":"Mendez","year":"2013"},{"key":"10.1016\/j.jcv.2020.104513_bib0030","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1556\/EuJMI.4.2014.1.2","article-title":"Human adenovirus: viral pathogen with increasing importance","volume":"4","author":"Ghebremedhin","year":"2014","journal-title":"Eur. J. Microbiol. Immunol. (Bp)"},{"key":"10.1016\/j.jcv.2020.104513_bib0035","doi-asserted-by":"crossref","first-page":"3723","DOI":"10.1128\/JCM.02103-15","article-title":"Multiplex molecular panels for diagnosis of gastrointestinal infection: performance, result interpretation, and cost-effectiveness","volume":"53","author":"Binnicker","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0040","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1017\/S0950268812002622","article-title":"Simultaneous detection of viral and bacterial enteric pathogens using the Seeplex(R) Diarrhea ACE detection system","volume":"141","author":"Coupland","year":"2013","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.jcv.2020.104513_bib0045","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.bdq.2014.07.001","article-title":"A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens","volume":"1","author":"Reddington","year":"2014","journal-title":"Biomol. Detect. Quantif."},{"key":"10.1016\/j.jcv.2020.104513_bib0050","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.cll.2015.02.006","article-title":"Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis","volume":"35","author":"Zhang","year":"2015","journal-title":"Clin. Lab. Med."},{"key":"10.1016\/j.jcv.2020.104513_bib0055","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1128\/JCM.02697-13","article-title":"Real-time PCR threshold cycle cutoffs help to identify agents causing acute childhood diarrhea in Zanzibar","volume":"52","author":"Elfving","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0060","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0166-0934(98)00168-2","article-title":"Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses","volume":"78","author":"Iturriza-Gomara","year":"1999","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2020.104513_bib0065","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1002\/jmv.20009","article-title":"Increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea","volume":"72","author":"Pang","year":"2004","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jcv.2020.104513_bib0070","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1128\/JCM.41.4.1548-1557.2003","article-title":"Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR","volume":"41","author":"Kageyama","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0075","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1128\/jcm.33.4.797-801.1995","article-title":"Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing","volume":"33","author":"Noel","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0080","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1128\/jcm.30.6.1365-1373.1992","article-title":"Identification of group A rotavirus gene 4 types by polymerase chain reaction","volume":"30","author":"Gentsch","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0085","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1128\/jcm.28.2.276-282.1990","article-title":"Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens","volume":"28","author":"Gouvea","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0090","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.jcv.2004.04.009","article-title":"Rotavirus genotyping: keeping up with an evolving population of human rotaviruses","volume":"31","author":"Iturriza-Gomara","year":"2004","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2020.104513_bib0095","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0166-0934(01)00404-9","article-title":"Genogroup-specific PCR primers for detection of Norwalk-like viruses","volume":"100","author":"Kojima","year":"2002","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2020.104513_bib0100","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1007\/s00705-013-1708-5","article-title":"Proposal for a unified norovirus nomenclature and genotyping","volume":"158","author":"Kroneman","year":"2013","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jcv.2020.104513_bib0105","doi-asserted-by":"crossref","first-page":"4523","DOI":"10.1128\/AEM.68.9.4523-4533.2002","article-title":"Environmental factors influencing human viral pathogens and their potential indicator organisms in the blue mussel, Mytilus edulis: the first Scandinavian report","volume":"68","author":"Hernroth","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0110","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.resmic.2003.09.013","article-title":"Quantification of human astroviruses in sewage using real-time RT-PCR","volume":"155","author":"Le Cann","year":"2004","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0115","first-page":"11","article-title":"Quantitative real-time polymerase chain reaction for the core facility using TaqMan and the Perkin-Elmer\/Applied Biosystems Division 7700 Sequence Detector","volume":"10","author":"Grove","year":"1999","journal-title":"J. Biomol. Tech."},{"key":"10.1016\/j.jcv.2020.104513_bib0120","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/JCM.02438-07","article-title":"Acute infantile gastroenteritis associated with human enteric viruses in Tunisia","volume":"46","author":"Sdiri-Loulizi","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0125","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jviromet.2012.09.021","article-title":"The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting rotavirus A and norovirus","volume":"187","author":"Dung","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2020.104513_bib0130","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.4269\/ajtmh.17-0473","article-title":"Coinfection with enteric pathogens in east african children with acute gastroenteritis-associations and interpretations","volume":"98","author":"Andersson","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.jcv.2020.104513_bib0135","doi-asserted-by":"crossref","first-page":"486","DOI":"10.4269\/ajtmh.17-0142","article-title":"Visceral Leishmaniasis and HIV Co-infection in Northwest Ethiopia: antiretroviral treatment and burden of disease among patients enrolled in HIV care","volume":"98","author":"van Griensven","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.jcv.2020.104513_bib0140","doi-asserted-by":"crossref","first-page":"4011","DOI":"10.1128\/JCM.01044-08","article-title":"Aichi virus, norovirus, astrovirus, enterovirus, and rotavirus involved in clinical cases from a French oyster-related gastroenteritis outbreak","volume":"46","author":"Le Guyader","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0145","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1007\/s10096-019-03646-4","article-title":"Multicenter evaluation of the new QIAstat Gastrointestinal Panel for the rapid syndromic testing of acute gastroenteritis","volume":"38","author":"Hannet","year":"2019","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jcv.2020.104513_bib0150","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jviromet.2019.03.010","article-title":"Performance evaluation of gastrointestinal viral ELIte panel multiplex RT-PCR assay for the diagnosis of rotavirus, adenovirus and astrovirus infection","volume":"268","author":"De Grazia","year":"2019","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2020.104513_bib0155","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.jcv.2013.09.028","article-title":"Epidemiology and genetic diversity of human astrovirus infection among hospitalized patients with acute diarrhea in Bangladesh from 2010 to 2012","volume":"58","author":"Afrad","year":"2013","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2020.104513_bib0160","doi-asserted-by":"crossref","first-page":"4306","DOI":"10.1128\/JCM.00797-10","article-title":"Outbreak of neonatal gastroenteritis associated with astrovirus serotype 1 at a hospital in Inner Mongolia, china","volume":"48","author":"Li","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0165","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1007\/s11262-013-1013-0","article-title":"Phylogenetic analysis of bovine astrovirus in Korean cattle","volume":"48","author":"Oem","year":"2014","journal-title":"Virus Genes"},{"key":"10.1016\/j.jcv.2020.104513_bib0170","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.vetmic.2015.05.002","article-title":"High prevalence and diversity of bovine astroviruses in the faeces of healthy and diarrhoeic calves in South West Scotland","volume":"178","author":"Sharp","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0175","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1128\/jcm.19.5.623-630.1984","article-title":"Astrovirus and Breda virus infections of dome cell epithelium of bovine ileum","volume":"19","author":"Woode","year":"1984","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104513_bib0180","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1007\/s00705-016-2798-7","article-title":"Temporal variation in the distribution of type-1 human astrovirus lineages in a settled population over 14 years","volume":"161","author":"De Grazia","year":"2016","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jcv.2020.104513_bib0185","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1471-2334-9-63","article-title":"Diagnosing norovirus-associated infectious intestinal disease using viral load","volume":"9","author":"Phillips","year":"2009","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.jcv.2020.104513_bib0190","doi-asserted-by":"crossref","first-page":"S50","DOI":"10.1097\/INF.0b013e3181967bee","article-title":"Rotavirus overview","volume":"28","author":"Bernstein","year":"2009","journal-title":"Pediatr. Infect. Dis. J."},{"key":"10.1016\/j.jcv.2020.104513_bib0195","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1007\/BF02849868","article-title":"Rotavirus gastroenteritis","volume":"22","author":"Leung","year":"2005","journal-title":"Adv. Ther."},{"key":"10.1016\/j.jcv.2020.104513_bib0200","first-page":"188","article-title":"Rotavirus gastroenteritis","volume":"6","author":"Leung","year":"1988","journal-title":"J. Diarrhoeal Dis. Res."}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653220302559?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653220302559?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T03:14:25Z","timestamp":1761362065000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653220302559"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":40,"alternative-id":["S1386653220302559"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2020.104513","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Assessing the burden of viral co-infections in acute gastroenteritis in children: An eleven-year-long investigation","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2020.104513","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104513"}}