{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:01:36Z","timestamp":1775779296661,"version":"3.50.1"},"reference-count":25,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T00:00:00Z","timestamp":1593043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004337","name":"Roche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004337","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.jcv.2020.104525","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T03:38:32Z","timestamp":1593229112000},"page":"104525","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Performance of Roche qualitative HEV assay on the cobas 6800 platform for quantitative measurement of HEV RNA"],"prefix":"10.1016","volume":"129","author":[{"given":"Viktoria","family":"Thodou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Bremer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olympia E.","family":"Anastasiou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9141-8001","authenticated-orcid":false,"given":"Markus","family":"Cornberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Maasoumy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heiner","family":"Wedemeyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.jcv.2020.104525_bib0005","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1128\/JCM.02334-16","article-title":"Hepatitis e virus (HEV) detection and quantification by a real-time reverse Transcription-PCR assay calibrated to the world health organization standard for HEV RNA","volume":"55","author":"Germer","year":"2017","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.jcv.2020.104525_bib0010","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/rmv.522","article-title":"Hepatitis e virus","volume":"17","author":"Panda","year":"2007","journal-title":"Rev. Med. Virol."},{"issue":"6","key":"10.1016\/j.jcv.2020.104525_bib0015","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.jinf.2018.09.012","article-title":"Epidemiologic estimates of hepatitis E virus infection in European countries","volume":"77","author":"Horn","year":"2018","journal-title":"J. Infect."},{"issue":"35\u201336","key":"10.1016\/j.jcv.2020.104525_bib0020","first-page":"577","article-title":"Hepatitis E in Germany--an under-reported infectious disease","volume":"111","author":"Pischke","year":"2014","journal-title":"Arztebl. Int."},{"issue":"10","key":"10.1016\/j.jcv.2020.104525_bib0025","first-page":"1836","article-title":"Hepatitis e virus infection as a possible cause of acute liver failure in Europe","volume":"13","author":"Manka","year":"2015","journal-title":"Clin. Gastroenterol. Hepatol. Off. Clin. Pract. J. Am. Gastroenterol. Assoc."},{"issue":"5","key":"10.1016\/j.jcv.2020.104525_bib0030","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1053\/j.gastro.2011.02.050","article-title":"Factors associated with chronic hepatitis in patients with hepatitis E virus infection who have received solid organ transplants","volume":"140","author":"Kamar","year":"2011","journal-title":"Gastroenterology"},{"issue":"8","key":"10.1016\/j.jcv.2020.104525_bib0035","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1111\/j.1600-6143.2008.02286.x","article-title":"Hepatitis E virus-related cirrhosis in kidney- and kidney-pancreas-transplant recipients","volume":"8","author":"Kamar","year":"2008","journal-title":"Am. J. Transplant. Off. J. Am. Soc. Transplant. Am. Soc. Transplant Surgeons"},{"issue":"8","key":"10.1016\/j.jcv.2020.104525_bib0040","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1056\/NEJMoa0706992","article-title":"Hepatitis E virus and chronic hepatitis in organ-transplant recipients","volume":"358","author":"Kamar","year":"2008","journal-title":"N. Engl. J. Med."},{"issue":"4","key":"10.1016\/j.jcv.2020.104525_bib0045","doi-asserted-by":"crossref","first-page":"467","DOI":"10.4142\/jvs.2013.14.4.467","article-title":"Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus","volume":"14","author":"Lee","year":"2013","journal-title":"J. Vet. Sci."},{"issue":"3","key":"10.1016\/j.jcv.2020.104525_bib0050","doi-asserted-by":"crossref","first-page":"e24","DOI":"10.1086\/654801","article-title":"Serologic assays specific to immunoglobulin M antibodies against hepatitis E virus: pangenotypic evaluation of performances","volume":"51","author":"Drobeniuc","year":"2010","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"issue":"4","key":"10.1016\/j.jcv.2020.104525_bib0055","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/j.jcv.2013.10.003","article-title":"Performance of anti-HEV assays for diagnosing acute hepatitis E in immunocompromised patients","volume":"58","author":"Abravanel","year":"2013","journal-title":"J. Clin. Virol. Off. Publ. Pan Am. Soc. Clin. Virol."},{"issue":"4","key":"10.1016\/j.jcv.2020.104525_bib0060","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1128\/JCM.02578-10","article-title":"Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance","volume":"49","author":"Baylis","year":"2011","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.jcv.2020.104525_bib0065","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1128\/JCM.05942-11","article-title":"Genotype 3 diversity and quantification of hepatitis E virus RNA","volume":"50","author":"Abravanel","year":"2012","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.jcv.2020.104525_bib0070","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1099\/jmm.0.000706","article-title":"Laboratory challenges in the diagnosis of hepatitis E virus","volume":"67","author":"Al-Sadeq","year":"2018","journal-title":"J. Med. Microbiol."},{"issue":"2","key":"10.1016\/j.jcv.2020.104525_bib0075","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1111\/trf.15058","article-title":"Hepatitis E virus blood donor NAT screening: as much as possible or as much as needed?","volume":"59","author":"Vollmer","year":"2019","journal-title":"Transfusion"},{"issue":"12","key":"10.1016\/j.jcv.2020.104525_bib0080","article-title":"Hepatitis E virus (HEV) in Scotland: evidence of recent increase in viral circulation in humans","volume":"23","author":"Thom","year":"2018","journal-title":"Euro Surveill. Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Dis. Bull."},{"issue":"Suppl 1","key":"10.1016\/j.jcv.2020.104525_bib0085","first-page":"1","article-title":"PCR-Screening of blood donations for hepatitis E with the cobas HEV test performed on the new Roche cobas 8800 platform in minipools of 6","volume":"42","author":"M\u00fcller","year":"2015","journal-title":"Transfus. Med. Hemother."},{"key":"10.1016\/j.jcv.2020.104525_bib0090","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.bdq.2015.01.005","article-title":"How good is a PCR efficiency estimate: recommendations for precise and robust qPCR efficiency assessments","volume":"3","author":"Svec","year":"2015","journal-title":"Biomol. Detect. Quantif."},{"key":"10.1016\/j.jcv.2020.104525_bib0095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jcv.2018.10.003","article-title":"Performance of a commercial assay for detecting and quantifying HEV RNA in faeces","volume":"109","author":"Abravanel","year":"2018","journal-title":"J. Clin. Virol. Off. Pub. Pan Am. Soc. Clin. Virol."},{"key":"10.1016\/j.jcv.2020.104525_bib0100","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1186\/1471-2334-12-142","article-title":"Seroprevalence of anti-hepatitis E virus (HEV) in a Korean population: comparison of two commercial anti-HEV assays","volume":"12","author":"Park","year":"2012","journal-title":"BMC Infect. Dis."},{"issue":"1","key":"10.1016\/j.jcv.2020.104525_bib0105","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0166-0934(99)00131-7","article-title":"Inhibitors of RT-PCR in serum","volume":"84","author":"Konet","year":"2000","journal-title":"J. Virol. Methods"},{"issue":"6","key":"10.1016\/j.jcv.2020.104525_bib0110","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1128\/JCM.00661-13","article-title":"Performance of two commercial assays for detecting hepatitis E virus RNA in acute or chronic infections","volume":"51","author":"Abravanel","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2020.104525_bib0115","doi-asserted-by":"crossref","first-page":"S124","DOI":"10.1016\/j.jcv.2015.07.289","article-title":"Clinical evaluation of a novel Fast-track diagnostics multiplex real-time PCR assay for detection and quantification of Hepatitis E virus","volume":"70","author":"Gruhn","year":"2015","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2020.104525_bib0120","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.coviro.2018.10.001","article-title":"Hepatitis E virus treatment and ribavirin therapy: viral mechanisms of nonresponse","volume":"32","author":"Todt","year":"2018","journal-title":"Curr. Opin. Virol."},{"issue":"6","key":"10.1016\/j.jcv.2020.104525_bib0125","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.jhep.2018.03.005","article-title":"EASL clinical practice guidelines on hepatitis e virus infection","volume":"68","author":"European Association for the Study of the Liver","year":"2018","journal-title":"J. Hepatol."}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653220302675?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653220302675?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T03:14:51Z","timestamp":1761362091000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653220302675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":25,"alternative-id":["S1386653220302675"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2020.104525","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Performance of Roche qualitative HEV assay on the cobas 6800 platform for quantitative measurement of HEV RNA","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2020.104525","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"104525"}}