{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:51:49Z","timestamp":1772121109615,"version":"3.50.1"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.jcv.2021.104764","type":"journal-article","created":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T12:11:23Z","timestamp":1613563883000},"page":"104764","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"special_numbering":"C","title":["Extraction-free RT-LAMP to detect SARS-CoV-2 is less sensitive but highly specific compared to standard RT-PCR in 101 samples"],"prefix":"10.1016","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3970-1608","authenticated-orcid":false,"given":"John J.","family":"Schellenberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Ormond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4948-4707","authenticated-orcid":false,"given":"Yoav","family":"Keynan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.jcv.2021.104764_bib0005","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Euro Surveill."},{"issue":"1","key":"10.1016\/j.jcv.2021.104764_bib0010","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1080\/22221751.2020.1719902","article-title":"Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan","volume":"9","author":"Chan","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"10.1016\/j.jcv.2021.104764_bib0015","series-title":"Serology-based and Molecular-based Tests Tracker","year":"2020"},{"key":"10.1016\/j.jcv.2021.104764_bib0020","series-title":"COVID-19 In Vitro Diagnostic Devices and Test Methods Database","year":"2020"},{"key":"10.1016\/j.jcv.2021.104764_bib0025","series-title":"Coronavirus Test Tracker: Commercially Available COVID-19 Diagnostic Tests. 360Dx.com","year":"2020"},{"issue":"2","key":"10.1016\/j.jcv.2021.104764_bib0030","first-page":"97","article-title":"COVID-19 diagnostic approaches: different roads to the same destination","volume":"31","author":"Kumar","year":"2020","journal-title":"Virus Dis."},{"issue":"September","key":"10.1016\/j.jcv.2021.104764_bib0035","article-title":"Put an end to asymptomatic testing, before the system gets overwhelmed","author":"Chagla","year":"2020","journal-title":"National Post"},{"issue":"3","key":"10.1016\/j.jcv.2021.104764_bib0040","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s40258-020-00579-4","article-title":"Why only test symptomatic patients? Consider random screening for COVID-19","volume":"18","author":"Padula","year":"2020","journal-title":"Appl. Health Econ. Health Policy"},{"key":"10.1016\/j.jcv.2021.104764_bib0045","doi-asserted-by":"crossref","DOI":"10.1056\/NEJMoa2006100","article-title":"Spread of SARS-CoV-2 in the icelandic population","author":"Gudbjartsson","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jcv.2021.104764_bib0050","article-title":"The effectiveness of population-wide, rapid antigen test based screening in reducing SARS-CoV-2 infection prevalence in Slovakia","author":"Pavelka","year":"2020","journal-title":"medRxiv."},{"key":"10.1016\/j.jcv.2021.104764_bib0055","article-title":"Comparison of the NeuMoDX, Diasorin Simplexa, Cepheid and Roche CDC SARS-CoV EUA assays using nasopharyngeal\/nasal swabs in universal transport media (UTM) and sputum and tracheal aspirates","author":"Tibbetts","year":"2020","journal-title":"bioRxiv."},{"issue":"8","key":"10.1016\/j.jcv.2021.104764_bib0060","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00783-20","article-title":"Clinical evaluation of three sample-to-Answer platforms for the detection of SARS-CoV-2","volume":"58","author":"Zhen","year":"2020","journal-title":"J. Clin. Microbiol."},{"issue":"1820","key":"10.1016\/j.jcv.2021.104764_bib0065","article-title":"Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples","volume":"11","author":"Alekseenko","year":"2021","journal-title":"Sci. Rep."},{"issue":"8","key":"10.1016\/j.jcv.2021.104764_bib0070","doi-asserted-by":"crossref","DOI":"10.3390\/biology9080182","article-title":"Loop-mediated isothermal amplification (LAMP): a rapid, sensitive, specific, and cost-effective point-of-Care test for coronaviruses in the context of COVID-19 pandemic","volume":"9","author":"Augustine","year":"2020","journal-title":"Biology"},{"key":"10.1016\/j.jcv.2021.104764_bib0075","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jviromet.2017.12.005","article-title":"Evaluation of clinical applicability of reverse transcription-loop-mediated isothermal amplification assay for detection and subtyping of Influenza A viruses","volume":"253","author":"Sharma","year":"2018","journal-title":"J. Virol. Method"},{"key":"10.1016\/j.jcv.2021.104764_bib0080","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.trac.2017.10.015","article-title":"Recombinase polymerase amplification: basics, applications and recent advances","volume":"98","author":"Lobato","year":"2018","journal-title":"Trends Anal. Chem."},{"issue":"12","key":"10.1016\/j.jcv.2021.104764_bib0085","doi-asserted-by":"crossref","first-page":"1806","DOI":"10.1261\/rna.072629.119","article-title":"RNA detection with high specificity and sensitivity using nested fluorogenic Mango NASBA","volume":"25","author":"Abdolahzadeh","year":"2019","journal-title":"RNA"},{"issue":"10","key":"10.1016\/j.jcv.2021.104764_bib0090","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01669-20","article-title":"Performance characteristics of a high throughput automated transcription mediated amplification test for SARS-CoV-2 detection","volume":"58","author":"Pham","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104764_bib0095","article-title":"Room temperature isothermal colorimetric padlock probe rolling circle amplification for viral RNA detection","author":"Huang","year":"2020","journal-title":"bioRxiv"},{"key":"10.1016\/j.jcv.2021.104764_bib0100","article-title":"Point-of-care testing for COVID-19 using SHERLOCK diagnostics","author":"Joung","year":"2020","journal-title":"medRxiv"},{"key":"10.1016\/j.jcv.2021.104764_bib0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2020.104428","article-title":"Comparison of cepheid xpert xpress and abbott ID now to Roche cobas for the rapid detection of SARS-CoV-2","volume":"128","author":"Smithgall","year":"2020","journal-title":"J. Clin. Virol."},{"issue":"1","key":"10.1016\/j.jcv.2021.104764_bib0110","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-020-19258-y","article-title":"An enhanced isothermal amplification assay for viral detection","volume":"11","author":"Qian","year":"2020","journal-title":"Nat. Commun."},{"issue":"eabc7075","key":"10.1016\/j.jcv.2021.104764_bib0115","article-title":"A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples","volume":"12","author":"Loan Dao Thi","year":"2020","journal-title":"Sci Trans Med."},{"key":"10.1016\/j.jcv.2021.104764_bib0120","article-title":"Rapid molecular detection of SARS-CoV-2 (COVID-19) virus RNA using colorimetric LAMP","author":"Zhang","year":"2020","journal-title":"medRxiv"},{"issue":"12","key":"10.1016\/j.jcv.2021.104764_bib0125","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0244882","article-title":"Optimizing direct RT-LAMP to detect transmissible SARS-CoV-2 from primary patient samples","volume":"15","author":"Dudley","year":"2020","journal-title":"PLoS One"},{"issue":"15","key":"10.1016\/j.jcv.2021.104764_bib0130","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21155380","article-title":"A rapid, simple, inexpensive, and mobile colorimetric assay COVID-19-LAMP for mass on-site screening of COVID-19","volume":"21","author":"Chow","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"7","key":"10.1016\/j.jcv.2021.104764_bib0135","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1093\/clinchem\/hvaa102","article-title":"Rapid detection of COVID-19 coronavirus using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform","volume":"66","author":"Yu","year":"2020","journal-title":"Clin. Chem."},{"issue":"1","key":"10.1016\/j.jcv.2021.104764_bib0140","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1186\/s12879-020-05484-8","article-title":"Detecting SARS-CoV-2 at point of care: preliminary data comparing loop-mediated isothermal amplification (LAMP) to polymerase chain reaction (PCR)","volume":"20","author":"\u00d6sterdahl","year":"2020","journal-title":"BMC Infect. Dis."},{"issue":"4","key":"10.1016\/j.jcv.2021.104764_bib0145","doi-asserted-by":"crossref","DOI":"10.1128\/msphere.00808-20","article-title":"Development and clinical application of a rapid and sensitive loop-mediated isothermal amplification test for SARS-CoV-2 infection","volume":"5","author":"Hu","year":"2020","journal-title":"mSphere"},{"key":"10.1016\/j.jcv.2021.104764_bib0150","doi-asserted-by":"crossref","first-page":"m4323","DOI":"10.1136\/bmj.m4323","article-title":"Rapid test missed over 50% of positive cases in Manchester pilot","volume":"371","author":"Iacobucci","year":"2020","journal-title":"BMJ"},{"key":"10.1016\/j.jcv.2021.104764_bib0155","doi-asserted-by":"crossref","DOI":"10.1093\/cid\/ciaa638","article-title":"Predicting infectious SARS-CoV-2 from diagnostic samples","author":"Bullard","year":"2020","journal-title":"Clin. Infect. Dis."},{"issue":"November","key":"10.1016\/j.jcv.2021.104764_bib0160","article-title":"Lucira health\u2019s At-Home covid test, explained","year":"2020","journal-title":"Bloomberg"},{"issue":"October","key":"10.1016\/j.jcv.2021.104764_bib0165","article-title":"COVID-19 diagnostics: how do saliva tests compare to swabs?","author":"Heidt","year":"2020","journal-title":"Scientist"},{"key":"10.1016\/j.jcv.2021.104764_bib0170","article-title":"Evaluation of a rapid antigen test (PanbioTM COVID-19 Ag rapid test device) for SARS-CoV-2 detection in asymptomatic close contacts of COVID-19 patients","author":"Torres","year":"2020","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.jcv.2021.104764_bib0175","article-title":"Evaluation of the PanbioTM rapid antigen test for SARS-CoV-2 in primary health care centers and test sites","author":"Bulilete","year":"2020","journal-title":"medRxiv"},{"key":"10.1016\/j.jcv.2021.104764_bib0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2020.104659","article-title":"Panbio antigen rapid test is reliable to diagnose SARS-CoV-2 infection in the first 7 days after the onset of symptoms","volume":"133","author":"Linares","year":"2020","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2021.104764_bib0185","article-title":"Evaluation of the rapid antigen test Panbio COVID-19 in saliva and nasal swabs in a population-based point-of-care study","author":"Agullo","year":"2020","journal-title":"J. Infect."},{"key":"10.1016\/j.jcv.2021.104764_bib0190","unstructured":"Harrison, A. FDA Issues Alert Regarding SARS-CoV-2 Viral Mutation to Health Care Providers and Clinical Laboratory Staff (Press release, Jan. 8, 2021). URL: https:\/\/www.fda.gov\/news-events\/press-announcements\/fda-issues-alert-regarding-sars-cov-2-viral-mutation-health-care-providers-and-clinical-laboratory, Accessed Oct. 15, 2020."},{"issue":"1","key":"10.1016\/j.jcv.2021.104764_bib0195","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-70812-6","article-title":"Genome-wide analysis of SARS-CoV-2 virus strains circulating worldwide implicates heterogeneity","volume":"10","author":"Islam","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jcv.2021.104764_bib0200","article-title":"COVID-19 CG: tracking SARS-CoV-2 mutations by locations and dates of interest","author":"Chen","year":"2020","journal-title":"bioRxiv"},{"issue":"3","key":"10.1016\/j.jcv.2021.104764_bib0205","doi-asserted-by":"crossref","first-page":"179","DOI":"10.2144\/btn-2020-0078","article-title":"Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride","volume":"69","author":"Zhang","year":"2020","journal-title":"BioTechniques"},{"issue":"11","key":"10.1016\/j.jcv.2021.104764_bib0210","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0238612","article-title":"Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay","volume":"15","author":"Schermer","year":"2020","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.jcv.2021.104764_bib0215","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1038\/s41591-020-0891-7","article-title":"\u2019Test, re-test, re-test\u2019: using inaccurate tests to greatly increase the accuracy of COVID-19 testing","volume":"26","author":"Ramdas","year":"2020","journal-title":"Nat. Med."},{"issue":"6","key":"10.1016\/j.jcv.2021.104764_bib0220","article-title":"Combined point-of-Care nucleic acid and antibody testing for SARS-CoV-2 following emergence of D614G spike variant","volume":"1","author":"Mlcochova","year":"2020","journal-title":"Cell Rep. Med."},{"key":"10.1016\/j.jcv.2021.104764_bib0225","article-title":"Evaluation of ID NOW and RT-PCR for detection of SARS-CoV-2 in an ambulatory population","author":"Tu","year":"2020","journal-title":"medRxiv"},{"key":"10.1016\/j.jcv.2021.104764_bib0230","article-title":"An extended laboratory validation study and comparative performance evaluation of the Abbott ID NOW TM COVID-19 assay in a coastal California tertiary care medical center","author":"Comer","year":"2020","journal-title":"medRxiv"},{"key":"10.1016\/j.jcv.2021.104764_bib0235","article-title":"Evaluation of the Panbio Covid-19 rapid antigen detection test device for the screening of patients with Covid-19","author":"Fenollar","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104764_bib0240","doi-asserted-by":"crossref","DOI":"10.1016\/j.eclinm.2020.100677","article-title":"Real-life validation of the Panbio COVID-19 antigen rapid test (Abbott) in community-dwelling subjects with symptoms of potential SARS-CoV-2 infection","volume":"31","author":"Gremmels","year":"2021","journal-title":"EClinicalMedicine"}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653221000317?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653221000317?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T03:13:45Z","timestamp":1761362025000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653221000317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":48,"alternative-id":["S1386653221000317"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2021.104764","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.12.07.20239558","asserted-by":"object"}]},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Extraction-free RT-LAMP to detect SARS-CoV-2 is less sensitive but highly specific compared to standard RT-PCR in 101 samples","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2021.104764","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104764"}}