{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:43:42Z","timestamp":1787237022958,"version":"build-2736575974"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2021,7]]},"DOI":"10.1016\/j.jcv.2021.104839","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T03:57:21Z","timestamp":1619150241000},"page":"104839","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Classification of HIV-1 virological treatment failure using the Roche cobas plasma separation card on cobas 8800 compared to dried blood spots on Abbott RealTime HIV-1"],"prefix":"10.1016","volume":"140","author":[{"given":"Lucia","family":"Hans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1326-5177","authenticated-orcid":false,"given":"Ed G.","family":"Marins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian O.","family":"Simon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieketseng","family":"Magubane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Seiverth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Carmona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcv.2021.104839_bib0001","series-title":"Consolidated Guidelines on the Use of Antiretroviral Drugs for Treating and Preventing HIV infection: Recommendations for a Public Health Approach \u20132nd ed","year":"2016"},{"key":"10.1016\/j.jcv.2021.104839_bib0002","series-title":"Guidelines for the Use of Antiretroviral Agents in Adults and Adolescents Living With HIV","year":"2018"},{"key":"10.1016\/j.jcv.2021.104839_bib0003","series-title":"European Guidelines for Treatment of HIV-Positive Adults in Europe, Version 10.0","year":"2019"},{"key":"10.1016\/j.jcv.2021.104839_bib0004","series-title":"90\u201390\u201390 - An ambitious Treatment Target to Help End the AIDS Epidemic","year":"2017"},{"key":"10.1016\/j.jcv.2021.104839_bib0005","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1093\/cid\/ciw001","article-title":"Scale-up of routine viral load testing in resource-poor settings: current and future implementation challenges","volume":"62","author":"Roberts","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.jcv.2021.104839_bib0006","doi-asserted-by":"crossref","first-page":"e86461","DOI":"10.1371\/journal.pone.0086461","article-title":"Systematic review of the use of dried blood spots for monitoring HIV viral load and for early infant diagnosis","volume":"9","author":"Smit","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jcv.2021.104839_bib0007","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1128\/JCM.01919-06","article-title":"Use of dried spots of whole blood, plasma, and mother\u2019s milk collected on filter paper for measurement of human immunodeficiency virus type 1 burden","volume":"45","author":"Ayele","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0008","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1128\/JCM.41.5.1888-1893.2003","article-title":"Multicenter evaluation of use of dried blood and plasma spot specimens in quantitative assays for human immunodeficiency virus RNA: measurement, precision, and RNA stability","volume":"41","author":"Brambilla","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0009","series-title":"20th Conference on Retroviruses and Opportunistic Infections","first-page":"608","article-title":"Use of finger-prick dried blood spots (DBS) for quantifying HIV viral load: a diagnostic accuracy study conducted in Thyolo, Malawi","author":"Metcalf","year":"2013"},{"key":"10.1016\/j.jcv.2021.104839_bib0010","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.arcmed.2005.03.010","article-title":"High correlation of human immunodeficiency virus type-1 viral load measured in dried-blood spot samples and in plasma under different storage conditions","volume":"36","author":"Alvarez-Munoz","year":"2005","journal-title":"Arch. Med. Res."},{"key":"10.1016\/j.jcv.2021.104839_bib0011","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.jviromet.2007.11.011","article-title":"Quantitation of HIV-1 RNA in dried blood spots by the real-time NucliSENS EasyQ HIV-1 assay in Senegal","volume":"148","author":"Kane","year":"2008","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2021.104839_bib0012","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1177\/135965350901400513","article-title":"Dried fluid spots for HIV type-1 viral load and resistance genotyping: a systematic review","volume":"14","author":"Hamers","year":"2009","journal-title":"Antivir. Ther."},{"key":"10.1016\/j.jcv.2021.104839_bib0013","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1128\/JCM.02860-13","article-title":"Field evaluation of dried blood spots for routine HIV-1 viral load and drug resistance monitoring in patients receiving antiretroviral therapy in Africa and Asia","volume":"52","author":"Monleau","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0014","series-title":"The ANRS 12235 study. 20th Conference on Retroviruses and Opportunistic Infections","article-title":"Dried blood spots (DBS) for HIV-1 viral load and drug resistance monitoring in pa)ents receiving HAART in Africa and Asia","author":"Ayouba","year":"2013"},{"key":"10.1016\/j.jcv.2021.104839_bib0015","first-page":"160","article-title":"Measurement of HIV-1 viral load for drug resistance surveillance using dried blood spots: literature review and modeling of contribution of DNA and RNA","volume":"16","author":"Parkin","year":"2014","journal-title":"AIDS Rev."},{"key":"10.1016\/j.jcv.2021.104839_bib0016","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jviromet.2011.07.001","article-title":"Comparison of HIV-1 detection in plasma specimens and dried blood spots using the Roche COBAS Ampliscreen HIV-1 test in Kisumu, Kenya","volume":"179","author":"Okonji","year":"2012","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2021.104839_bib0017","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1111\/j.1468-1293.2007.00484.x","article-title":"Evaluation of the use of dried spots and of different storage conditions of plasma for HIV-1 RNA quantification","volume":"8","author":"Amellal","year":"2007","journal-title":"HIV Med."},{"key":"10.1016\/j.jcv.2021.104839_bib0018","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1128\/JCM.00425-17","article-title":"Evaluation of the Aptima HIV-1 Quant Dx Assay for HIV-1 RNA quantitation in different biological specimen types","volume":"55","author":"Yek","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0019","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1128\/JCM.02354-08","article-title":"Dried-plasma transport using a novel matrix and collection system for human immunodeficiency virus and hepatitis C virus virologic testing","volume":"47","author":"Lloyd","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0020","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jcv.2010.08.017","article-title":"Comparative effectiveness of dried plasma HIV-1 viral load testing in Brazil using ViveST for sample collection","volume":"49","author":"Zanoni","year":"2010","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2021.104839_bib0021","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1128\/JCM.01848-13","article-title":"Higher specificity of nucleic acid sequence-based amplification isothermal technology than of real-time PCR for quantification of HIV-1 RNA on dried blood spots","volume":"52","author":"Mercier-Delarue","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0022","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1128\/JCM.03519-13","article-title":"Prospective evaluation of diagnostic accuracy of dried blood spots from finger prick samples for determination of HIV-1 load with the NucliSENS Easy-Q HIV-1 version 2.0 assay in Malawi","volume":"52","author":"Fajardo","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0023","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jcv.2017.05.002","article-title":"HIV-1 viral load measurement in venous blood and fingerprick blood using Abbott RealTime HIV-1 DBS assay","volume":"92","author":"Tang","year":"2017","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2021.104839_bib0024","series-title":"WHO Prequalification of Diagnostics Programme Public Report","year":"2017"},{"key":"10.1016\/j.jcv.2021.104839_bib0025","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.jviromet.2011.09.015","article-title":"A novel RealTime HIV-1 qualitative assay for the detection of HIV-1 nucleic acids in dried blood spots and plasma","volume":"178","author":"Huang","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2021.104839_bib0026","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jcv.2015.01.022","article-title":"A simple method to elute cell-free HIV from dried blood spots improves their usefulness for monitoring therapy","volume":"65","author":"Wu","year":"2015","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2021.104839_bib0027","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1128\/JCM.00176-17","article-title":"A simple phosphate-buffered-saline-based extraction method improves specificity of HIV viral load monitoring using dried blood spots","volume":"55","author":"Makadzange","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0028","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jviromet.2017.10.014","article-title":"Comparison of different nucleic acid preparation methods to improve specific HIV-1 RNA isolation for viral load testing on dried blood spots","volume":"251","author":"Guichet","year":"2018","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jcv.2021.104839_bib0029","first-page":"57","article-title":"Separation of plasma from whole blood by use of the cobas plasma separation card: a compelling alternative to dried blood spots for quantification of HIV-1 viral load","author":"Carmona","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0030","unstructured":"Vaks J. Probability of detection of target nucleic acid sequence with PCR assay in molecular diagnostics: math model derivation, validation and applications. joint statistical meetings. Baltimore, MD 2017, pp. 2460\u201376."},{"key":"10.1016\/j.jcv.2021.104839_bib0031","series-title":"Joint Statistical Meetings. Vancouver, BC","first-page":"529","article-title":"New method of evaluation of limit of detection (LoD) in molecular diagnostics","author":"Vaks","year":"2018"},{"key":"10.1016\/j.jcv.2021.104839_bib0032","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jcv.2017.09.007","article-title":"Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep\/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load","volume":"96","author":"Wirden","year":"2017","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jcv.2021.104839_bib0033","first-page":"56","article-title":"Multicenter evaluation of two next-generation HIV-1 quantitation assays, Aptima Quant Dx and Cobas 6800, in comparison to the RealTime HIV-1 reference assay","author":"Wiesmann","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2021.104839_bib0034","series-title":"Cost-Effectiveness of HIV Viral Load Sample Collection and Testing Methods in Malawi","author":"Babigumira","year":"2018"},{"key":"10.1016\/j.jcv.2021.104839_bib0035","doi-asserted-by":"crossref","first-page":"e438","DOI":"10.1016\/S2352-3018(18)30132-2","article-title":"Viral suppression and HIV transmission in serodiscordant male couples: an international, prospective, observational, cohort study","volume":"5","author":"Bavinton","year":"2018","journal-title":"Lancet HIV"},{"key":"10.1016\/j.jcv.2021.104839_bib0036","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1056\/NEJMoa1600693","article-title":"Antiretroviral therapy for the prevention of HIV-1 transmission","volume":"375","author":"Cohen","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jcv.2021.104839_bib0037","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1001\/jama.2016.5148","article-title":"Sexual Activity without condoms and risk of HIV transmission in serodifferent couples when the HIV-positive partner is using suppressive antiretroviral therapy","volume":"316","author":"Rodger","year":"2016","journal-title":"JAMA"}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653221001062?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653221001062?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T03:14:33Z","timestamp":1761362073000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653221001062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7]]},"references-count":37,"alternative-id":["S1386653221001062"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2021.104839","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2021,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Classification of HIV-1 virological treatment failure using the Roche cobas plasma separation card on cobas 8800 compared to dried blood spots on Abbott RealTime HIV-1","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2021.104839","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"104839"}}