{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:07:52Z","timestamp":1790046472790,"version":"4.0.1"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008877","name":"Biosensors International Group","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008877","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2023,4]]},"DOI":"10.1016\/j.jcv.2023.105402","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:49:16Z","timestamp":1676393356000},"page":"105402","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"special_numbering":"C","title":["Rapid differential diagnosis of SARS-CoV-2, influenza A\/B and respiratory syncytial viruses: Validation of a novel RT-PCR assay"],"prefix":"10.1016","volume":"161","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9608-8892","authenticated-orcid":false,"given":"Alexander","family":"Domnich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca","family":"Bruzzone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo-Simone","family":"Trombetta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"De Pace","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Ricucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Varesano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giada","family":"Garzillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde","family":"Ogliastro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Orsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Icardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"16","key":"10.1016\/j.jcv.2023.105402_bib0001","doi-asserted-by":"crossref","first-page":"17","DOI":"10.2807\/1560-7917.ES.2018.23.16.17-00454","article-title":"Impact of infectious diseases on population health using incidence-based disability-adjusted life years (DALYs): results from the Burden of Communicable Diseases in Europe study, European Union and European Economic Area countries, 2009 to 2013","volume":"23","author":"Cassini","year":"2018","journal-title":"Euro Surveill."},{"issue":"2","key":"10.1016\/j.jcv.2023.105402_bib0002","doi-asserted-by":"crossref","first-page":"S143","DOI":"10.1093\/infdis\/jiac211","article-title":"The burden of respiratory syncytial virus lower respiratory tract disease in infants in the United States: a synthesis","volume":"226","author":"Sim\u00f5es","year":"2022","journal-title":"J. Infect. Dis."},{"issue":"11","key":"10.1016\/j.jcv.2023.105402_bib0003","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2021.26.11.2100221","article-title":"Very little influenza in the WHO European Region during the 2020\/21 season, weeks\u200940 2020 to 8 2021","volume":"26","author":"Adlhoch","year":"2021","journal-title":"Euro Surveill."},{"issue":"29","key":"10.1016\/j.jcv.2023.105402_bib0004","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2021.26.29.2100639","article-title":"Low levels of respiratory syncytial virus activity in Europe during the 2020\/21 season: what can we expect in the coming summer and autumn\/winter?","volume":"26","author":"van Summeren","year":"2021","journal-title":"Euro Surveill."},{"key":"10.1016\/j.jcv.2023.105402_bib0005","unstructured":"Australian Government. Department of Health and Aged Care. Australian influenza surveillance report - 2022 influenza season in Australia. Available online: https:\/\/www1.health.gov.au\/internet\/main\/publishing.nsf\/Content\/cda-ozflu-2022.htm."},{"issue":"3","key":"10.1016\/j.jcv.2023.105402_bib0006","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1002\/ppul.25802","article-title":"The return of RSV during the COVID-19 pandemic","volume":"57","author":"Ferrero","year":"2022","journal-title":"Pediatr. Pulmonol."},{"issue":"9","key":"10.1016\/j.jcv.2023.105402_bib0007","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1093\/infdis\/jiab485","article-title":"Quantifying the impact of COVID-19 nonpharmaceutical interventions on influenza transmission in the United States","volume":"224","author":"Qi","year":"2021","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2023.105402_bib0008","first-page":"jiac192","article-title":"Waning immunity against respiratory syncytial virus during the COVID-19 pandemic","author":"Reicherz","year":"2022","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jcv.2023.105402_bib0009","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.908525","article-title":"Influenza A, influenza B, and SARS-CoV-2 similarities and differences - A focus on diagnosis","volume":"13","author":"Havasi","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jcv.2023.105402_bib0010","first-page":"hcac205","article-title":"Respiratory syncytial virus pneumonia in an immunocompetent adult: an important differential diagnosis of COVID-19","author":"Tone","year":"2022","journal-title":"QJM"},{"issue":"10334","key":"10.1016\/j.jcv.2023.105402_bib0011","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1016\/S0140-6736(22)00383-X","article-title":"SARS-CoV-2 co-infection with influenza viruses, respiratory syncytial virus, or adenoviruses","volume":"399","author":"Swets","year":"2022","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.jcv.2023.105402_bib0012","doi-asserted-by":"crossref","first-page":"e00512","DOI":"10.1128\/JCM.00512-20","article-title":"Laboratory diagnosis of COVID-19: current issues and challenges","volume":"58","author":"Tang","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2023.105402_bib0013","series-title":"Report For the 11th Meeting of the WHO Working Group For the Molecular Detection and Subtyping of Influenza Viruses and the Use of NGS in GISRS","year":"2021"},{"issue":"1","key":"10.1016\/j.jcv.2023.105402_bib0014","doi-asserted-by":"crossref","first-page":"S63","DOI":"10.1093\/infdis\/jiab600","article-title":"Performance assessment of a rapid molecular respiratory syncytial virus point-of-care test: a prospective community study in older adults","volume":"226","author":"Zuurbier","year":"2022","journal-title":"J. Infect. Dis."},{"issue":"9","key":"10.1016\/j.jcv.2023.105402_bib0015","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.3390\/jcm11092465","article-title":"Comparative diagnostic accuracy of the STANDARD M10 assay for the molecular diagnosis of SARS-CoV-2 in the point-of-care and critical care settings","volume":"11","author":"Domnich","year":"2022","journal-title":"J. Clin. Med."},{"issue":"3","key":"10.1016\/j.jcv.2023.105402_bib0016","article-title":"Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection","volume":"3","author":"Dinnes","year":"2021","journal-title":"Cochrane Database Syst. Rev."},{"issue":"14","key":"10.1016\/j.jcv.2023.105402_bib0017","doi-asserted-by":"crossref","first-page":"3942","DOI":"10.3390\/jcm11143942","article-title":"Evaluation of four fully integrated molecular assays for the detection of respiratory viruses during the co-circulation of SARS-CoV-2, influenza and RSV","volume":"11","author":"Farfour","year":"2022","journal-title":"J. Clin. Med."},{"issue":"4","key":"10.1016\/j.jcv.2023.105402_bib0018","article-title":"Multi-center evaluation of Cepheid Xpert\u00ae Xpress SARS-CoV-2\/Flu\/RSV molecular point-of-care test","volume":"1","author":"Sluimer","year":"2021","journal-title":"J. Clin. Virol. Plus."},{"key":"10.1016\/j.jcv.2023.105402_bib0019","unstructured":"European Commission, Medical Device Coordination Group. Guidance on performance evaluation of SARS-CoV-2 in vitro diagnostic medical devices, revision 1 \u2013 February 2022. Available online: https:\/\/health.ec.europa.eu\/system\/files\/2022-02\/mdcg_2021-21_en.pdf."},{"issue":"9","key":"10.1016\/j.jcv.2023.105402_bib0020","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1007\/s10096-012-1602-1","article-title":"Methods and recommendations for evaluating and reporting a new diagnostic test","volume":"31","author":"Hess","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jcv.2023.105402_bib0021","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jcv.2018.05.006","article-title":"Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus","volume":"105","author":"Gimferrer","year":"2018","journal-title":"J. Clin. Virol."},{"issue":"1","key":"10.1016\/j.jcv.2023.105402_bib0022","doi-asserted-by":"crossref","first-page":"91","DOI":"10.7326\/0003-4819-126-1-199701010-00026","article-title":"Sensitivity\" and \"specificity\" reconsidered: the meaning of these terms in analytical and diagnostic settings","volume":"126","author":"Saah","year":"1997","journal-title":"Ann. Intern. Med."},{"issue":"11","key":"10.1016\/j.jcv.2023.105402_bib0023","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2016-012799","article-title":"STARD 2015 guidelines for reporting diagnostic accuracy studies: explanation and elaboration","volume":"6","author":"Cohen","year":"2016","journal-title":"BMJ. Open."},{"issue":"10","key":"10.1016\/j.jcv.2023.105402_bib0024","doi-asserted-by":"crossref","first-page":"5798","DOI":"10.1002\/jmv.27111","article-title":"European multicenter evaluation of Xpert\u00ae Xpress SARS-CoV-2\/Flu\/RSV test","volume":"93","author":"Wolters","year":"2021","journal-title":"J. Med. Virol."},{"issue":"4","key":"10.1016\/j.jcv.2023.105402_bib0025","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.02965-20","article-title":"Evaluation of the Xpert Xpress SARS-CoV-2\/Flu\/RSV assay for simultaneous detection of SARS-CoV-2, influenza A and B viruses, and respiratory syncytial virus in nasopharyngeal specimens","volume":"59","author":"Leung","year":"2021","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.jcv.2023.105402_bib0026","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.02955-20","article-title":"Multicenter evaluation of the Cepheid Xpert Xpress SARS-CoV-2\/Flu\/RSV test","volume":"59","author":"Mostafa","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jcv.2023.105402_bib0027","article-title":"Evaluation of the respiratory NeuMoDx\u2122 Flu A-B\/RSV\/SARS-CoV-2 Vantage and Alinity m Resp-4-Plex assays","volume":"150\u2013151","author":"Quinton","year":"2022","journal-title":"J. Clin. Virol."},{"issue":"8","key":"10.1016\/j.jcv.2023.105402_bib0028","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.3390\/diagnostics12081998","article-title":"STANDARD M10 SARS-CoV-2 assay for rapid detection of SARS-CoV-2: comparison of four real-time PCR assays","volume":"12","author":"Jeong","year":"2022","journal-title":"Diagnostics (Basel)"},{"issue":"1","key":"10.1016\/j.jcv.2023.105402_bib0029","article-title":"Evaluation of STANDARD\u2122 M10 SARS-CoV-2 assay as a diagnostic tool for SARS-CoV-2 in nasopharyngeal or oropharyngeal swab samples","volume":"3","author":"Parakatselaki","year":"2023","journal-title":"J. Clin. Virol. Plus."},{"key":"10.1016\/j.jcv.2023.105402_bib0030","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.999783","article-title":"Performance evaluation of a new on-demand molecular test for the rapid identification of severe acute respiratory syndrome coronavirus 2 in pediatric and adult patients","volume":"13","author":"Colagrossi","year":"2022","journal-title":"Front. Microbiol."},{"issue":"2","key":"10.1016\/j.jcv.2023.105402_bib0031","doi-asserted-by":"crossref","first-page":"360","DOI":"10.3947\/ic.2022.0047","article-title":"Performance of STANDARD\u2122 M10 SARS-CoV-2 assay for the diagnosis of COVID-19 from a nasopharyngeal swab","volume":"54","author":"Ham","year":"2022","journal-title":"Infect. Chemother."},{"issue":"6","key":"10.1016\/j.jcv.2023.105402_bib0032","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.3390\/v14061316","article-title":"SARS-CoV-2 Delta variant N gene mutations reduce sensitivity to the TaqPath COVID-19 multiplex molecular diagnostic assay","volume":"14","author":"Holland","year":"2022","journal-title":"Viruses"},{"issue":"1","key":"10.1016\/j.jcv.2023.105402_bib0033","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s12889-021-12426-9","article-title":"Characterizing the transmission patterns of seasonal influenza in Italy: lessons from the last decade","volume":"22","author":"Trentini","year":"2022","journal-title":"BMC Public Health"},{"issue":"1","key":"10.1016\/j.jcv.2023.105402_bib0034","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1186\/s13054-019-2491-9","article-title":"Influenza virus-related critical illness: prevention, diagnosis, treatment","volume":"23","author":"Chow","year":"2019","journal-title":"Crit. Care."},{"issue":"3A","key":"10.1016\/j.jcv.2023.105402_bib0035","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0002-9343(97)00014-4","article-title":"Prevention and treatment of respiratory syncytial virus and parainfluenza viruses in immunocompromised patients","volume":"102","author":"Englund","year":"1997","journal-title":"Am. J. Med."},{"key":"10.1016\/j.jcv.2023.105402_bib0036","article-title":"COVID antibody drugs work best when given as early as possible","author":"Callaway","year":"2022","journal-title":"Nature"}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653223000252?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653223000252?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T03:17:30Z","timestamp":1761362250000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653223000252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4]]},"references-count":36,"alternative-id":["S1386653223000252"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2023.105402","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2023,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid differential diagnosis of SARS-CoV-2, influenza A\/B and respiratory syncytial viruses: Validation of a novel RT-PCR assay","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2023.105402","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"105402"}}