{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:25:09Z","timestamp":1788909909745,"version":"build-2803163510"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011099","name":"Deutsche Gesellschaft f\u00fcr Internationale Zusammenarbeit","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011099","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["ERANet17\/HLH-0231"],"award-info":[{"award-number":["ERANet17\/HLH-0231"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003042","name":"Else Kr\u00f6ner-Fresenius-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007865","name":"University of Texas Medical Branch","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006456","name":"Bundesministerium f\u00fcr Wirtschaftliche Zusammenarbeit und Entwicklung","doi-asserted-by":"publisher","award":["81262528"],"award-info":[{"award-number":["81262528"]}],"id":[{"id":"10.13039\/501100006456","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Clinical Virology"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.jcv.2025.105872","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T02:41:57Z","timestamp":1758595317000},"page":"105872","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Syndromic approach for rapid detection and differentiation of human pathogenic alphaviruses"],"prefix":"10.1016","volume":"181","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9163-2406","authenticated-orcid":false,"given":"Carlo","family":"Fischer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anges","family":"Yadouleton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Mauricio","family":"Cabada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miladi Gatty","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Piche-Ovares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephane","family":"Sohou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar Augusto","family":"Cabezas S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia T.","family":"Bozza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Paquita Garc\u00eda","family":"Mendoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Gotuzzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Augusto","family":"Bozza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3509-0232","authenticated-orcid":false,"given":"Jan Felix","family":"Drexler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcv.2025.105872_bib1","unstructured":"Launch of the Global Arbovirus Initiative [press release], 2022."},{"issue":"5","key":"10.1016\/j.jcv.2025.105872_bib2","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1038\/s41564-019-0376-y","article-title":"Past and future spread of the arbovirus vectors Aedes aegypti and Aedes albopictus","volume":"4","author":"Kraemer","year":"2019","journal-title":"Nat. Microbiol."},{"issue":"1","key":"10.1016\/j.jcv.2025.105872_bib3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S1473-3099(19)30697-8","article-title":"Viral emergence and immune interplay in flavivirus vaccines","volume":"20","author":"Fischer","year":"2020","journal-title":"Lancet Infect. Dis."},{"issue":"3","key":"10.1016\/j.jcv.2025.105872_bib4","doi-asserted-by":"crossref","first-page":"e101","DOI":"10.1016\/S1473-3099(16)30518-7","article-title":"Epidemic arboviral diseases: priorities for research and public health","volume":"17","author":"Wilder-Smith","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jcv.2025.105872_bib5","unstructured":"Chikungunya cases by Country or Territory Cumulative Cases [Internet], Pan American Health Organization, 2024 [cited January 25, 2024]. Available from: \u3008https:\/\/www3.paho.org\/data\/index.php\/en\/mnu-topics\/chikv-en\/550-chikv-weekly-en.html\u3009."},{"issue":"9","key":"10.1016\/j.jcv.2025.105872_bib6","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.cmi.2021.05.047","article-title":"Challenges towards serologic diagnostics of emerging arboviruses","volume":"27","author":"Fischer","year":"2021","journal-title":"Clin. Microbiol. Infect."},{"issue":"6","key":"10.1016\/j.jcv.2025.105872_bib7","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1099\/jgv.0.001072","article-title":"ICTV virus taxonomy profile: togaviridae","volume":"99","author":"Chen","year":"2018","journal-title":"J. Gen. Virol."},{"issue":"6","key":"10.1016\/j.jcv.2025.105872_bib8","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/s41579-022-00825-7","article-title":"A molecular understanding of alphavirus entry and antibody protection","volume":"21","author":"Kim","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"issue":"1","key":"10.1016\/j.jcv.2025.105872_bib9","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s12879-020-4834-1","article-title":"Prioritising pathogens for the management of severe febrile patients to improve clinical care in low- and middle-income countries","volume":"20","author":"Osborn","year":"2020","journal-title":"BMC Infect. Dis."},{"issue":"6","key":"10.1016\/j.jcv.2025.105872_bib10","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1590\/0074-0276140108","article-title":"Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil","volume":"109","author":"Zuchi","year":"2014","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"6","key":"10.1016\/j.jcv.2025.105872_bib11","doi-asserted-by":"crossref","first-page":"721","DOI":"10.2217\/fvl.11.50","article-title":"Endemic Venezuelan equine encephalitis in the Americas: hidden under the dengue umbrella","volume":"6","author":"Aguilar","year":"2011","journal-title":"Future Virol."},{"issue":"3","key":"10.1016\/j.jcv.2025.105872_bib12","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofz001","article-title":"Chikungunya and O\u2032nyong-nyong viruses in Uganda: implications for diagnostics","volume":"6","author":"Clements","year":"2019","journal-title":"Open Forum Infect. Dis."},{"issue":"9","key":"10.1016\/j.jcv.2025.105872_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004073","article-title":"Syndromic approach to arboviral diagnostics for global travelers as a basis for infectious disease surveillance","volume":"9","author":"Cleton","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.jcv.2025.105872_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(25)00110-0","article-title":"The spatiotemporal ecology of Oropouche virus across Latin America: a multidisciplinary, laboratory-based, modelling study","author":"Fischer","year":"2025","journal-title":"Lancet Infect. Dis."},{"issue":"1","key":"10.1016\/j.jcv.2025.105872_bib15","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12866-017-1080-9","article-title":"Improved detection of genus-specific Alphavirus using a generic TaqMan(R) assay","volume":"17","author":"Giry","year":"2017","journal-title":"BMC Microbiol."},{"issue":"9","key":"10.1016\/j.jcv.2025.105872_bib16","doi-asserted-by":"crossref","first-page":"3386","DOI":"10.1128\/JCM.00317-10","article-title":"Detection of all species of the genus Alphavirus by reverse transcription-PCR with diagnostic sensitivity","volume":"48","author":"Grywna","year":"2010","journal-title":"J. Clin. Microbiol."},{"issue":"12","key":"10.1016\/j.jcv.2025.105872_bib17","doi-asserted-by":"crossref","first-page":"880","DOI":"10.2471\/BLT.16.175950","article-title":"Assay optimization for molecular detection of Zika virus","volume":"94","author":"Corman","year":"2016","journal-title":"Bull. World Health Organ."},{"issue":"1","key":"10.1016\/j.jcv.2025.105872_bib18","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jcv.2009.02.010","article-title":"Development and validation of real-time one-step reverse transcription-PCR for the detection and typing of Dengue viruses","volume":"45","author":"Leparc-Goffart","year":"2009","journal-title":"J. Clin. Virol."},{"issue":"7","key":"10.1016\/j.jcv.2025.105872_bib19","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.3201\/eid2207.160326","article-title":"Single-Reaction multiplex reverse transcription PCR for detection of Zika, Chikungunya, and Dengue viruses","volume":"22","author":"Waggoner","year":"2016","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.jcv.2025.105872_bib20","doi-asserted-by":"crossref","first-page":"121","DOI":"10.4269\/ajtmh.17-0027","article-title":"Development of a real-time reverse transcription polymerase chain reaction for O\u2032nyong-nyong virus and evaluation with clinical and mosquito specimens from Kenya","volume":"97","author":"Waggoner","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.jcv.2025.105872_bib21","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-36043-6","article-title":"A quantitative reverse transcription-polymerase chain reaction for detection of Getah virus","volume":"8","author":"Sam","year":"2018","journal-title":"Sci. Rep."},{"issue":"12","key":"10.1016\/j.jcv.2025.105872_bib22","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00152-23","article-title":"Real-time RT-PCR for Venezuelan equine encephalitis complex, Madariaga, and Eastern equine encephalitis viruses: application in human and mosquito public health surveillance in Panama","volume":"61","author":"Carrera","year":"2023","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.jcv.2025.105872_bib23","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.02676-20","article-title":"Variable sensitivity of SARS-CoV-2 molecular detection in European expert laboratories: external quality assessment, June and July 2020","volume":"59","author":"Fischer","year":"2021","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"10.1016\/j.jcv.2025.105872_bib24","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3201\/eid2502.180166","article-title":"Differential shedding and antibody kinetics of Zika and Chikungunya viruses, Brazil","volume":"25","author":"Bozza","year":"2019","journal-title":"Emerg. Infect. Dis."},{"issue":"5","key":"10.1016\/j.jcv.2025.105872_bib25","doi-asserted-by":"crossref","first-page":"995","DOI":"10.3201\/eid3105.241694","article-title":"Venezuelan equine encephalitis, Peruvian Amazon, 2020","volume":"31","author":"Piche-Ovares","year":"2025","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.jcv.2025.105872_bib26","doi-asserted-by":"crossref","first-page":"6735","DOI":"10.1038\/s41467-021-26707-9","article-title":"Model-based assessment of Chikungunya and O\u2032nyong-nyong virus circulation in Mali in a serological cross-reactivity context","volume":"12","author":"Hoze","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jcv.2025.105872_bib27","article-title":"GloPID-R report on Chikungunya, O\u2032nyong-nyong and Mayaro virus, part 2: epidemiological distribution of O\u2032nyong-nyong virus","volume":"172","author":"Pezzi","year":"2019","journal-title":"Antivir. Res."},{"issue":"3","key":"10.1016\/j.jcv.2025.105872_bib28","doi-asserted-by":"crossref","first-page":"622","DOI":"10.4269\/ajtmh.18-0407","article-title":"Etiologies of acute undifferentiated febrile illness in Bangkok, Thailand","volume":"100","author":"Luvira","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"2","key":"10.1016\/j.jcv.2025.105872_bib29","doi-asserted-by":"crossref","first-page":"e256","DOI":"10.1016\/S2214-109X(24)00419-4","article-title":"Emergence and spread of the SARS-CoV-2 omicron (BA.1) variant across Africa: an observational study","volume":"13","author":"Fischer","year":"2025","journal-title":"Lancet Glob. Health"},{"issue":"7","key":"10.1016\/j.jcv.2025.105872_bib30","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.29788","article-title":"An improved alphaviruses-specific RT-qPCR facilitates monitoring and prevention of alphaviruses","volume":"96","author":"Xie","year":"2024","journal-title":"J. Med. Virol."},{"issue":"43","key":"10.1016\/j.jcv.2025.105872_bib31","article-title":"Venezuelan equine encephalitis virus non-structural protein 3 dictates superinfection exclusion in mammalian cells","volume":"2","author":"Hick","year":"2024","journal-title":"npj Viruses"}],"container-title":["Journal of Clinical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653225001143?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1386653225001143?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T15:30:27Z","timestamp":1764257427000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1386653225001143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":31,"alternative-id":["S1386653225001143"],"URL":"https:\/\/doi.org\/10.1016\/j.jcv.2025.105872","relation":{},"ISSN":["1386-6532"],"issn-type":[{"value":"1386-6532","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Syndromic approach for rapid detection and differentiation of human pathogenic alphaviruses","name":"articletitle","label":"Article Title"},{"value":"Journal of Clinical Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcv.2025.105872","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"105872"}}