{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T22:24:39Z","timestamp":1761431079871,"version":"build-2065373602"},"reference-count":31,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["celltherapyjournal.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cytotherapy"],"published-print":{"date-parts":[[2014,11]]},"DOI":"10.1016\/j.jcyt.2014.05.004","type":"journal-article","created":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T15:30:28Z","timestamp":1403623828000},"page":"1545-1557","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of a flow cytometry\u2013based pulse-width assay for detection of aggregates in cellular therapeutics to be infused by catheter"],"prefix":"10.1016","volume":"16","author":[{"given":"Darren H.M.","family":"Hickerson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah Storrie","family":"White","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Nieman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norin","family":"Meadows","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy","family":"Gentry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra J.","family":"Foster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew E.","family":"Balber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Fiordalisi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"James M.","family":"Hinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"first-page":"8","year":"2010","series-title":"Guidance for Industry: Cellular Therapy for Cardiac Disease","key":"10.1016\/j.jcyt.2014.05.004_bib1"},{"key":"10.1016\/j.jcyt.2014.05.004_bib2","unstructured":"Study of ALD-401 Via Intracarotid Infusion in Ischemic Stroke Subjects. ClinicalTrials.gov identifier: NCT01273337; clinical trial identification number IS-001, Submitted January 2011, currently listed as active, not recruiting."},{"key":"10.1016\/j.jcyt.2014.05.004_bib3","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.ahj.2011.11.020","article-title":"Randomized, double-blind pilot study of transendocardial injection of autologous aldehyde dehydrogenase-bright stem cells in patients with ischemic heart failure","volume":"163","author":"Perin","year":"2012","journal-title":"Am Heart J"},{"key":"10.1016\/j.jcyt.2014.05.004_bib4","doi-asserted-by":"crossref","first-page":"2248","DOI":"10.1002\/stem.1206","article-title":"Umbilical cord blood-derived aldehyde dehydrogenase-expressing progenitor cells promote recovery from acute ischemic injury","volume":"30","author":"Putman","year":"2012","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2014.05.004_bib5","first-page":"1060","article-title":"A\u00a0randomized, controlled study of autologous therapy with bone marrow-derived aldehyde dehydrogenase bright cells in patients with critical limb ischemia","volume":"78","author":"Perin","year":"2011","journal-title":"Catheter Cardiovasc Interv"},{"key":"10.1016\/j.jcyt.2014.05.004_bib6","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1002\/stem.613","article-title":"Concise review: aldehyde dehydrogenase bright stem and progenitor cell populations from normal tissues: characteristics, activities, and emerging uses in regenerative medicine","volume":"29","author":"Balber","year":"2011","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2014.05.004_bib7","article-title":"Mechanisms of action of human aldehyde dehydrogenase bright cells in therapy of cardiovascular diseases: expression analysis of angiogenic factors and aldehyde dehydrogenase isozymes","author":"White","year":"2011","journal-title":"J\u00a0Stem Cell Res Ther"},{"key":"10.1016\/j.jcyt.2014.05.004_bib8","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1111\/j.1751-7133.2009.00101.x","article-title":"Bone marrow-derived aldehyde dehydrogenase-bright stem and progenitor cells for ischemic repair","volume":"15","author":"Keller","year":"2009","journal-title":"Congest Heart Fail"},{"key":"10.1016\/j.jcyt.2014.05.004_bib9","doi-asserted-by":"crossref","first-page":"5340","DOI":"10.1182\/blood-2008-04-154567","article-title":"Revascularization of ischemic limbs after transplantation of human bone marrow cells with high aldehyde dehydrogenase activity","volume":"113","author":"Capoccia","year":"2009","journal-title":"Blood"},{"key":"10.1016\/j.jcyt.2014.05.004_bib10","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1038\/mt.2009.204","article-title":"Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities","volume":"17","author":"Vauchez","year":"2009","journal-title":"Mol Ther"},{"key":"10.1016\/j.jcyt.2014.05.004_bib11","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1634\/stemcells.2007-0429","article-title":"Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity","volume":"26","author":"Hess","year":"2008","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2014.05.004_bib12","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1080\/14653240701466347","article-title":"Isolation of early hematopoietic cells, including megakaryocyte progenitors, in the ALDH-bright cell population of cryopreserved, banked UC blood","volume":"9","author":"Gentry","year":"2007","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2014.05.004_bib13","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.expneurol.2009.10.010","article-title":"Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection","volume":"221","author":"Barbosa da Fonseca","year":"2010","journal-title":"Exp Neurol"},{"key":"10.1016\/j.jcyt.2014.05.004_bib14","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.jvir.2010.01.008","article-title":"Intraarterial autologous implantation of adult stem cells for patients with Parkinson disease","volume":"21","author":"Brazzini","year":"2010","journal-title":"J\u00a0Vasc Interv Radiol"},{"key":"10.1016\/j.jcyt.2014.05.004_bib15","first-page":"5","article-title":"A\u00a0rapid and reliable method of counting neurons and other cells in brain tissue: a comparison of flow cytometry and manual counting methods","volume":"4","author":"Collins","year":"2010","journal-title":"Front Neuroanat"},{"key":"10.1016\/j.jcyt.2014.05.004_bib16","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s11022-004-2382-5","article-title":"Coulter counter use in the enumeration of muscle and fat stem cells","volume":"25","author":"Fernyhough","year":"2003","journal-title":"Methods Cell Sci"},{"key":"10.1016\/j.jcyt.2014.05.004_bib17","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1183\/09031936.94.07081527","article-title":"Electronic cell counting to measure total cell numbers in bronchoalveolar lavage fluid","volume":"7","author":"Heaney","year":"1994","journal-title":"Eur Respir J"},{"key":"10.1016\/j.jcyt.2014.05.004_bib18","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/0014-4894(72)90063-X","article-title":"Counting methods for low concentrations of trypanosomes in blood, II: accuracy of the indirect method","volume":"32","author":"Creemers","year":"1972","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.jcyt.2014.05.004_bib19","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1177\/27.1.374594","article-title":"Laser flow cytometric light scatter and fluorescence pulse width and pulse rise-time sizing of mammalian cells","volume":"27","author":"Leary","year":"1979","journal-title":"J\u00a0Histochem Cytochem"},{"key":"10.1016\/j.jcyt.2014.05.004_bib20","first-page":"577","article-title":"Flow cytofluorimetry: discrimination between single cells and cell aggregates by direct size measurements","volume":"19","author":"Sharpless","year":"1975","journal-title":"Acta Cytol"},{"key":"10.1016\/j.jcyt.2014.05.004_bib21","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1093\/ajcp\/96.5.615","article-title":"Improved sensitivity and resolution in the flow cytometric DNA analysis of human solid tumor specimens: use of in vitro fine-needle aspiration and uniform staining reagents","volume":"96","author":"Bach","year":"1991","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.jcyt.2014.05.004_bib22","first-page":"5096","article-title":"Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements","volume":"53","author":"Gong","year":"1993","journal-title":"Cancer Res"},{"key":"10.1016\/j.jcyt.2014.05.004_bib23","first-page":"S72","article-title":"DNA ploidy and cell cycle analysis in breast cancer","volume":"120","author":"Ross","year":"2003","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.jcyt.2014.05.004_bib24","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1002\/cyto.1171","article-title":"Doublet discrimination in DNA cell-cycle analysis","volume":"46","author":"Wersto","year":"2001","journal-title":"Cytometry"},{"issue":"1","key":"10.1016\/j.jcyt.2014.05.004_bib25","doi-asserted-by":"crossref","DOI":"10.1177\/24.1.1254921","article-title":"Estimation of cell size from pulse shape in flow cytofluorometry","volume":"24","author":"Sharpless","year":"1976","journal-title":"J\u00a0Histochem Cytochem"},{"key":"10.1016\/j.jcyt.2014.05.004_bib26","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/cyto.990060211","article-title":"Single-cell analysis of the relationship among transferrin receptors, proliferation, and cell cycle phase in K562 cells","volume":"6","author":"Rudolph","year":"1985","journal-title":"Cytometry"},{"key":"10.1016\/j.jcyt.2014.05.004_bib27","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1002\/cyto.990140410","article-title":"Forward scatter pulse width signals resolve multiple populations of endosomes","volume":"14","author":"Hammond","year":"1993","journal-title":"Cytometry"},{"issue":"1","key":"10.1016\/j.jcyt.2014.05.004_bib28","first-page":"23","article-title":"Pulse width for particle sizing","volume":"1","author":"Hoffman","year":"2009","journal-title":"Curr Protoc Cytom"},{"key":"10.1016\/j.jcyt.2014.05.004_bib29","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1080\/14653240701466321","article-title":"Modification of a commercial cell sorter to support efficient and reliable preparation of ALDH-bright cells for clinical use","volume":"9","author":"Hickerson","year":"2007","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2014.05.004_bib30","series-title":"Biostatistical Analysis","first-page":"261","author":"Zar","year":"1984"},{"key":"10.1016\/j.jcyt.2014.05.004_bib31","unstructured":"IUPAC. Compendium of Chemical Terminology, 2nd edition. (the \u201cGold Book\u201d). Compiled by McNaught AD, Wilkinson A. Oxford: Blackwell Scientific Publications; 1997. XML on-line corrected version: http:\/\/goldbook.iupac.org (2006-) created by Nic M, Jirat J, Kosata B; updates compiled by Jenkins A."}],"container-title":["Cytotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324914006021?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324914006021?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T22:19:49Z","timestamp":1761430789000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1465324914006021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":31,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["S1465324914006021"],"URL":"https:\/\/doi.org\/10.1016\/j.jcyt.2014.05.004","relation":{},"ISSN":["1465-3249"],"issn-type":[{"type":"print","value":"1465-3249"}],"subject":[],"published":{"date-parts":[[2014,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a flow cytometry\u2013based pulse-width assay for detection of aggregates in cellular therapeutics to be infused by catheter","name":"articletitle","label":"Article Title"},{"value":"Cytotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcyt.2014.05.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}