{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:35:03Z","timestamp":1790235303015,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"F.E.S.R. Regione Liguria","award":["161"],"award-info":[{"award-number":["161"]}]},{"DOI":"10.13039\/100007388","name":"Compagnia di San Paolo","doi-asserted-by":"publisher","award":["2012.0312"],"award-info":[{"award-number":["2012.0312"]}],"id":[{"id":"10.13039\/100007388","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["celltherapyjournal.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cytotherapy"],"published-print":{"date-parts":[[2016,3]]},"DOI":"10.1016\/j.jcyt.2015.11.020","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T14:34:28Z","timestamp":1454682868000},"page":"438-451","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["A humanized system to expand in vitro amniotic fluid-derived stem cells intended for clinical application"],"prefix":"10.1016","volume":"18","author":[{"given":"Daniela","family":"Martinelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui Cruz","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Mogni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Benelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maddalena","family":"Mastrogiacomo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Coviello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranieri","family":"Cancedda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Gentili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jcyt.2015.11.020_bib0010","doi-asserted-by":"crossref","DOI":"10.1038\/nbt1274","article-title":"Isolation of amniotic stem cell lines with potential for therapy","author":"De Coppi","year":"2007","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0015","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-62703-508-8_16","article-title":"Isolation of c-Kit+ human amniotic fluid stem cells from second trimester","author":"Pozzobon","year":"2013","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0020","doi-asserted-by":"crossref","DOI":"10.1002\/jcb.20886","article-title":"Mesenchymal stem cells as trophic mediators","author":"Caplan","year":"2006","journal-title":"J Cell Biochem"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0025","doi-asserted-by":"crossref","DOI":"10.1161\/01.RES.0000118601.37875.AC","article-title":"Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms","author":"Kinnaird","year":"2004","journal-title":"Circ Res"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0030","doi-asserted-by":"crossref","DOI":"10.1634\/stemcells.2007-1122","article-title":"In search of the in vivo identity of mesenchymal stem cells","author":"da Silva Meirelles","year":"2008","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcyt.2012.11.005","article-title":"Mesenchymal stromal cells: misconceptions and evolving concepts","author":"Phinney","year":"2013","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0040","doi-asserted-by":"crossref","DOI":"10.2217\/rme.09.12","article-title":"Novel sources of fetal stem cells: where do they fit on the developmental continuum?","author":"Pappa","year":"2009","journal-title":"Regen Med"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0045","doi-asserted-by":"crossref","DOI":"10.1517\/14712598.2011.587800","article-title":"The potential of amniotic fluid stem cells for cellular therapy and tissue engineering","author":"Klemmt","year":"2011","journal-title":"Expert Opin Biol Ther"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0050","doi-asserted-by":"crossref","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0055","doi-asserted-by":"crossref","DOI":"10.1182\/blood-2007-02-069716","article-title":"Immunomodulatory properties of mesenchymal stromal cells","author":"Nauta","year":"2007","journal-title":"Blood"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0060","doi-asserted-by":"crossref","DOI":"10.1016\/S0091-679X(08)00005-8","article-title":"Characterization of human amniotic fluid stem cells and their pluripotential capability","author":"Perin","year":"2008","journal-title":"Methods Cell Biol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0065","doi-asserted-by":"crossref","first-page":"53","DOI":"10.14573\/altex.2010.1.53","article-title":"Gstraunthaler G. Serum-free cell culture: the serum-free media interactive online database","volume":"27","author":"Brunner","year":"2010","journal-title":"ALTEX"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2010.03.016","article-title":"Optimization of chemically defined cell culture media\u2013replacing fetal bovine serum in mammalian in vitro methods","author":"van der Valk","year":"2010","journal-title":"Toxicol Vitr"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0075","first-page":"105","article-title":"In vitro enhancement of peripheral blood mononuclear cell natural killer activity following short term incubation with fetal calf serum","volume":"12","author":"Lauer","year":"1983","journal-title":"J Clin Lab Immunol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0080","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1002\/ijc.2910220317","article-title":"Specific cytotoxic lymphocytes against syngeneic tumors are generated in culture in the presence of syngeneic, but not xenogeneic, serum","volume":"22","author":"Fogel","year":"1978","journal-title":"Int J Cancer"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.joca.2006.05.008","article-title":"Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis","author":"Akeda","year":"2006","journal-title":"Osteoarthr Cartil"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0090","doi-asserted-by":"crossref","first-page":"3095","DOI":"10.1016\/S0142-9612(03)00114-5","article-title":"Platelet-derived growth factors enhance proliferation of human stromal stem cells","volume":"24","author":"Lucarelli","year":"2003","journal-title":"Biomaterials"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0095","doi-asserted-by":"crossref","DOI":"10.1902\/jop.2004.75.9.1255","article-title":"Platelet-released supernatant increases matrix metalloproteinase-2 production, migration, proliferation, and tube formation of human umbilical vascular endothelial cells","author":"Kandler","year":"2004","journal-title":"J Periodontol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0100","doi-asserted-by":"crossref","DOI":"10.3109\/09537104.2013.803529","article-title":"Biological activity of a standardized freeze-dried platelet derivative to be used as cell culture medium supplement","author":"Muraglia","year":"2014","journal-title":"Platelets"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0105","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S1084952102000599","article-title":"Development of platelet secretory granules","volume":"13","author":"King","year":"2002","journal-title":"Semin Cell Dev Biol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0110","doi-asserted-by":"crossref","DOI":"10.1159\/000354061","article-title":"Platelet lysate as replacement for fetal bovine serum in mesenchymal stromal cell cultures","author":"Bieback","year":"2013","journal-title":"Transfus Med Hemother"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0115","doi-asserted-by":"crossref","first-page":"e26535","DOI":"10.1371\/journal.pone.0026535","article-title":"Cloned, CD117 selected human amniotic fluid stem cells are capable of modulating the immune response","volume":"6","author":"Moorefield","year":"2011","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0120","doi-asserted-by":"crossref","DOI":"10.3324\/haematol.2010.031633","article-title":"Relevance of the mevalonate biosynthetic pathway in the regulation of bone marrow mesenchymal stromal cell-mediated effects on T-cell proliferation and B-cell survival","author":"Musso","year":"2011","journal-title":"Haematologica"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2010.12.028","article-title":"Recruitment of host's progenitor cells to sites of human amniotic fluid stem cells implantation","author":"Mirabella","year":"2011","journal-title":"Biomaterials"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0130","doi-asserted-by":"crossref","first-page":"4435","DOI":"10.1073\/pnas.72.11.4435","article-title":"Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro","volume":"72","author":"Shin","year":"1975","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2011.01.071","article-title":"Amniotic liquid derived stem cells as reservoir of secreted angiogenic factors capable of stimulating neo-arteriogenesis in an ischemic model","author":"Mirabella","year":"2011","journal-title":"Biomaterials"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0140","doi-asserted-by":"crossref","DOI":"10.1002\/term.119","article-title":"Platelet lysate favours in vitro expansion of human bone marrow stromal cells for bone and cartilage engineering","author":"Zaky","year":"2008","journal-title":"J Tissue Eng Regen Med"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0145","doi-asserted-by":"crossref","DOI":"10.1089\/ten.tea.2012.0225","article-title":"Dual effect of platelet lysate on human articular cartilage: a maintenance of chondrogenic potential and a transient proinflammatory activity followed by an inflammation resolution","author":"Pereira","year":"2013","journal-title":"Tissue Eng Part A"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2013.08.018","article-title":"The effect of platelet lysate on osteoblast proliferation associated with a transient increase of the inflammatory response in bone regeneration","author":"Ruggiu","year":"2013","journal-title":"Biomaterials"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0155","article-title":"Platelet lysate induces in vitro wound healing of human keratinocytes associated with a strong proinflammatory response","author":"El Backly","year":"2011","journal-title":"Tissue Eng Part A"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.biocel.2013.07.024","article-title":"Adipogenic potential in human mesenchymal stem cells strictly depends on adult or foetal tissue harvest","author":"Ragni","year":"2013","journal-title":"Int J Biochem Cell Biol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0165","doi-asserted-by":"crossref","DOI":"10.1182\/blood-2009-05-219907","article-title":"Clinical-grade production of human mesenchymal stromal cells: occurrence of aneuploidy without transformation","author":"Tarte","year":"2010","journal-title":"Blood"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0170","doi-asserted-by":"crossref","DOI":"10.1634\/stemcells.2005-0330","article-title":"Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson's disease","author":"Weiss","year":"2006","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0175","doi-asserted-by":"crossref","first-page":"e35512","DOI":"10.1371\/journal.pone.0035512","article-title":"Activation of regulatory T cells during inflammatory response is not an exclusive property of stem cells","volume":"7","author":"Gosemann","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0180","article-title":"Dissecting paracrine effectors for mesenchymal stem cells","author":"Bruno","year":"2013","journal-title":"Adv Biochem Eng Biotechnol"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0185","doi-asserted-by":"crossref","DOI":"10.1089\/scd.2011.0639","article-title":"Proangiogenic soluble factors from amniotic fluid stem cells mediate the recruitment of endothelial progenitors in a model of ischemic fasciocutaneous flap","author":"Mirabella","year":"2012","journal-title":"Stem Cells Dev"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0190","doi-asserted-by":"crossref","DOI":"10.1182\/blood-2003-04-1291","article-title":"Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation","author":"In't Anker","year":"2003","journal-title":"Blood"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0195","doi-asserted-by":"crossref","first-page":"e54747","DOI":"10.1371\/journal.pone.0054747","article-title":"Spindle shaped human mesenchymal stem\/stromal cells from amniotic fluid promote neovascularization","volume":"8","author":"Roubelakis","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0200","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s12015-013-9494-8","article-title":"Platelet-rich plasma (PRP) promotes fetal mesenchymal stem\/stromal cell migration and wound healing process","volume":"10","author":"Roubelakis","year":"2014","journal-title":"Stem Cell Rev Reports"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.scr.2013.02.006","article-title":"Amniotic fluid stem cells in a bone microenvironment: driving host angiogenic response","author":"Mirabella","year":"2013","journal-title":"Stem Cell Res"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0210","doi-asserted-by":"crossref","DOI":"10.2217\/rme.10.96","article-title":"Effect of platelet-rich plasma on dental stem cells derived from human impacted third molars","author":"Lee","year":"2011","journal-title":"Regen Med"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0215","doi-asserted-by":"crossref","DOI":"10.1002\/jor.20896","article-title":"Endothelial cells incubated with platelet-rich plasma express PDGF-B and ICAM-1 and induce bone marrow stromal cell migration","author":"Cenni","year":"2009","journal-title":"J Orthop Res"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0220","doi-asserted-by":"crossref","DOI":"10.1089\/ten.tec.2013.0061","article-title":"Essential components for ex vivo proliferation of mesenchymal stromal cells","author":"Fekete","year":"2014","journal-title":"Tissue Eng Part C Methods"},{"key":"10.1016\/j.jcyt.2015.11.020_bib0225","doi-asserted-by":"crossref","first-page":"5308","DOI":"10.1016\/j.biomaterials.2012.04.007","article-title":"Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation","volume":"33","author":"Murphy","year":"2012","journal-title":"Biomaterials"}],"container-title":["Cytotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324915011536?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324915011536?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T22:23:33Z","timestamp":1761431013000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1465324915011536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["S1465324915011536"],"URL":"https:\/\/doi.org\/10.1016\/j.jcyt.2015.11.020","relation":{},"ISSN":["1465-3249"],"issn-type":[{"value":"1465-3249","type":"print"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A humanized system to expand in vitro amniotic fluid-derived stem cells intended for clinical application","name":"articletitle","label":"Article Title"},{"value":"Cytotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcyt.2015.11.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}