{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T09:02:43Z","timestamp":1772096563835,"version":"3.50.1"},"reference-count":76,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"NIAMS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000146","name":"NSF CBET","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000146","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007904","name":"Texas A&M University System","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007904","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.es","clinicalkey.com.au","clinicalkey.com","celltherapyjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cytotherapy"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.jcyt.2024.01.009","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T14:37:40Z","timestamp":1708007860000},"page":"372-382","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Scalable manufacture of therapeutic mesenchymal stromal cell products on customizable microcarriers in vertical wheel bioreactors that improve direct visualization, product harvest, and cost"],"prefix":"10.1016","volume":"26","author":[{"given":"Andrew","family":"Haskell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berkley P.","family":"White","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Rogers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"Goebel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan G.","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew E.","family":"Syvyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela A.","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather A.","family":"Barreda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua","family":"Benton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar R.","family":"Benavides","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujata","family":"Dalal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EunHye","family":"Bae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen","family":"Maitland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zivko","family":"Nikolov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryang Hwa","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Kaunas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl A.","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"7","key":"10.1016\/j.jcyt.2024.01.009_bib0001","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1002\/stem.3016","article-title":"Concise review: mesenchymal stem cells: from roots to boost","volume":"37","author":"Andrzejewska","year":"2019","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jcyt.2016.09.008","article-title":"The exciting prospects of new therapies with mesenchymal stromal cells","volume":"19","author":"Prockop","year":"2017","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0003","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.1002\/stem.1806","article-title":"Are clinical trials with mesenchymal stem\/progenitor cells too far ahead of the science? Lessons from experimental hematology","volume":"32","author":"Prockop","year":"2014","journal-title":"Stem Cells"},{"issue":"3","key":"10.1016\/j.jcyt.2024.01.009_bib0004","doi-asserted-by":"crossref","first-page":"386","DOI":"10.3390\/biom12030386","article-title":"Potential of using infrapatellar\u2013fat\u2013pad\u2013derivedmesenchymal stem cells for therapy in degenerative arthritis: chondrogenesis, exosomes, and transcription regulation","volume":"12","author":"Liao","year":"2022","journal-title":"Biomolecules"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0005","doi-asserted-by":"crossref","first-page":"8830834","DOI":"10.1155\/2021\/8830834","article-title":"Recent developed strategies for enhancing chondrogenic differentiation of MSC: impact on MSC-based therapy for cartilage regeneration","volume":"2021","author":"Zha","year":"2021","journal-title":"Stem Cells International"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0006","doi-asserted-by":"crossref","first-page":"132ra155","DOI":"10.1126\/scitranslmed.3003396","article-title":"Human mesenchymal stem cell-derived matrices for enhanced osteoregeneration","volume":"4","author":"Zeitouni","year":"2012","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0007","doi-asserted-by":"crossref","first-page":"243","DOI":"10.2174\/1574888X11308030009","article-title":"A systematic review on preclinical and clinical studies on the use of scaffolds for bone repair in skeletal defects","volume":"8","author":"Crowley","year":"2013","journal-title":"Curr Stem Cell Res Ther"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0008","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s40001-023-01034-5","article-title":"Emerging role of mesenchymal stem\/stromal cells (MSCs) and MSCs-derived exosomes in bone- and joint-associated musculoskeletal disorders: a new frontier","volume":"28","author":"Gerami","year":"2023","journal-title":"Eur J Med Res"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0009","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.2106\/JBJS.H.00303","article-title":"Mesenchymal stem cell concentration and bone repair: potential pitfalls from bench to bedside","volume":"91","author":"Cuomo","year":"2009","journal-title":"J Bone Joint Surg Am"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0010","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/jor.1100160202","article-title":"Bone regeneration by implantation of purified, culture-expanded human mesenchymal stem cells","volume":"16","author":"Bruder","year":"1998","journal-title":"J Orthop Res"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0011","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1634\/stemcells.2005-0567","article-title":"In vivo bone formation by human bone marrow stromal cells: reconstruction of the mouse calvarium and mandible","volume":"24","author":"Mankani","year":"2006","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0012","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.2106\/00004623-200310000-00010","article-title":"Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect","volume":"85","author":"Arinzeh","year":"2003","journal-title":"J Bone Joint Surg Am"},{"issue":"4","key":"10.1016\/j.jcyt.2024.01.009_bib0013","doi-asserted-by":"crossref","first-page":"284","DOI":"10.2174\/1574888X12666170915121137","article-title":"A systematic review of clinical studies investigating mesenchymal stem cells for fracture non-union and bone defects","volume":"13","author":"Killington","year":"2018","journal-title":"Curr Stem Cell Res Ther"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0014","doi-asserted-by":"crossref","first-page":"312","DOI":"10.2174\/1574888X11666160614104500","article-title":"The use of growth factors and mesenchymal stem cells in orthopaedics: in particular, their use in fractures and non-unions: a systematic review","volume":"12","author":"Sinclair","year":"2017","journal-title":"Curr Stem Cell Res Ther"},{"issue":"Suppl. 1","key":"10.1016\/j.jcyt.2024.01.009_bib0015","doi-asserted-by":"crossref","first-page":"S63","DOI":"10.1016\/j.injury.2020.02.070","article-title":"Early efficacy evaluation of mesenchymal stromal cells (MSC) combined to biomaterials to treat long bone non-unions","volume":"51","author":"Gomez-Barrena","year":"2020","journal-title":"Injury"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0016","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1146\/annurev-bioeng-070909-105309","article-title":"Mesenchymal stem cells as therapeutics","volume":"12","author":"Parekkadan","year":"2010","journal-title":"Ann Rev Biomed Eng"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0017","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1016\/j.biopha.2018.11.056","article-title":"Amelioration of clinical symptoms of patients with refractory rheumatoid arthritis following treatment with autologous bone marrow-derived mesenchymal stem cells: A successful clinical trial in Iran","volume":"109","author":"Ghoryani","year":"2019","journal-title":"Biomed Pharmacother"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0018","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/S0140-6736(16)31203-X","article-title":"Expanded allogeneic adipose-derived mesenchymal stem cells (Cx601) for complex perianal fistulas in Crohn's disease: a phase 3 randomised, double-blind controlled trial","volume":"388","author":"Pan\u00e9s","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0019","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.4161\/cc.6.23.5095","article-title":"Biochemical heterogeneity of mesenchymal stem cell populations: clues to their therapeutic efficacy","volume":"6","author":"Phinney","year":"2007","journal-title":"Cell Cycle"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0020","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1089\/scd.2009.0288","article-title":"Functional differences between mesenchymal stem cell populations are reflected by their transcriptome","volume":"19","author":"Jansen","year":"2010","journal-title":"Stem Cells Dev"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0021","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.jcyt.2017.11.013","article-title":"Variation in primary and culture-expanded cells derived from connective tissue progenitors in human bone marrow space, bone trabecular surface and adipose tissue","volume":"20","author":"Qadan","year":"2018","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0022","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s00441-015-2116-9","article-title":"Response of stem cells from different origins to biphasic calcium phosphate bioceramics","volume":"361","author":"Lobo","year":"2015","journal-title":"Cell Tissue Res"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0023","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1093\/cvr\/cvr113","article-title":"Human relevance of pre-clinical studies in stem cell therapy: systematic review and meta-analysis of large animal models of ischaemic heart disease","volume":"91","author":"van der Spoel","year":"2011","journal-title":"Cardiovasc Res"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0024","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.bbmt.2011.07.023","article-title":"Long-term complications, immunologic effects, and role of passage for outcome in mesenchymal stromal cell therapy","volume":"18","author":"von Bahr","year":"2012","journal-title":"Biol Blood Marrow Transplant"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0025","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement","volume":"8","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0026","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.jcyt.2022.07.010","article-title":"An International Society for Cell and Gene Therapy Mesenchymal Stromal Cells Committee editorial on overcoming limitations in clinical trials of mesenchymal stromal cell therapy for coronavirus disease-19: time for a global registry","volume":"24","author":"Weiss","year":"2022","journal-title":"Cytotherapy"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0027","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1073\/pnas.1423008112","article-title":"MSCs derived from iPSCs with a modified protocol are tumor-tropic but have much less potential to promote tumors than bone marrow MSCs","volume":"112","author":"Zhao","year":"2014","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0028","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1002\/stem.1084","article-title":"Derivation of mesenchymal stem cells from human induced pluripotent stem cells cultured on synthetic substrates","volume":"30","author":"Villa-Diaz","year":"2012","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0029","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/9601623","article-title":"Generation and applications of induced pluripotent stem cell-derived mesenchymal stem cells","volume":"2018","author":"Zhao","year":"2018","journal-title":"Stem Cells Int"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0030","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1089\/scd.2013.0111","article-title":"Generation of functional mesenchymal stem cells from different induced pluripotent stem cell lines","volume":"23","author":"Hynes","year":"2014","journal-title":"Stem Cells Dev"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0031","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1186\/s13287-015-0137-7","article-title":"Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity","volume":"6","author":"Kang","year":"2015","journal-title":"Stem Cell Res Ther"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0032","first-page":"7","article-title":"Meeting lot-size challenges of manufacturing adherent cells for therapy","volume":"10","author":"Rowley","year":"2012","journal-title":"Bioprocess Int"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0033","doi-asserted-by":"crossref","unstructured":"C. A. Gregory, Prockop, D.J., in Culture of human stem cells, R. I. Freshney, Stacey, G.N., Averback, J.M., editor. (John Wiley & Sons, Hoboken, NJ, 2007 207-232.","DOI":"10.1002\/9780470167526.ch9"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0034","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.bej.2018.02.018","article-title":"Technologies for large-scale umbilical cord-derived MSC expansion: experimental performance and cost of goods analysis","volume":"135","author":"Mizukami","year":"2018","journal-title":"Biochem Eng J"},{"issue":"8","key":"10.1016\/j.jcyt.2024.01.009_bib0035","article-title":"Scalable manufacturing of human mesenchymal stromal cells in the vertical-wheel bioreactor system: an experimental and economic approach","volume":"14","author":"de Sousa Pinta","year":"2019","journal-title":"Biotechnol J"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0036","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1002\/bit.25008","article-title":"Allogeneic cell therapy bioprocess economics and optimization: single-use cell expansion technologies","volume":"111","author":"Simaria","year":"2013","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0037","doi-asserted-by":"crossref","first-page":"4760414","DOI":"10.1155\/2016\/4760414","article-title":"Theoretical and practical issues that are relevant when scaling Up hMSC microcarrier production processes","volume":"2016","author":"Jossen","year":"2016","journal-title":"Stem Cells Int"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0038","article-title":"Disposable rocking bioreactors: recent applications and progressive perspectives","author":"Wierzchowski","year":"2023","journal-title":"Trends Biotechnol"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0039","doi-asserted-by":"crossref","DOI":"10.1002\/biot.201800716","article-title":"Scalable manufacturing of human mesenchymal stromal cells in the vertical-wheel bioreactor system: an experimental and economic approach","volume":"14","author":"de Sousa Pinto","year":"2019","journal-title":"Biotechnol J"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0040","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.memsci.2014.12.041","article-title":"Filtration methodologies for the clarification and concentration of human mesenchymal stem cells","volume":"478","author":"Chuna","year":"2015","journal-title":"J Membr Sci"},{"issue":"1","key":"10.1016\/j.jcyt.2024.01.009_bib0041","doi-asserted-by":"crossref","first-page":"12481","DOI":"10.1038\/s41598-018-31019-y","article-title":"Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions","volume":"8","author":"Moloudi","year":"2018","journal-title":"Sci Rep"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0042","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1002\/sctm.21-0151","article-title":"A scalable system for generation of mesenchymal stem cells derived from induced pluripotent cells employing bioreactors and degradable microcarriers","volume":"10","author":"Rogers","year":"2021","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0043","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0282298","article-title":"Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth","volume":"18","author":"Benavides","year":"2023","journal-title":"PLoS One"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0044","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ab.2004.02.002","article-title":"An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction","volume":"329","author":"Gregory","year":"2004","journal-title":"Anal Biochem"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0045","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1089\/ars.2012.4536","article-title":"Increased mitochondrial activity in BMP7-treated brown adipocytes, due to increased CPT1- and CD36-mediated fatty acid uptake","volume":"19","author":"Townsend","year":"2013","journal-title":"Antioxid Redox Signal"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0046","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.1038\/s41467-020-16646-2","article-title":"Characterization of a pluripotent stem cell-derived matrix with powerful osteoregenerative capabilities","volume":"11","author":"McNeill","year":"2020","journal-title":"Nat Commun"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0047","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/bs.mcb.2019.10.009","article-title":"Preparation of osteogenic matrices from cultured cells","volume":"156","author":"Gregory","year":"2020","journal-title":"Methods Cell Biol"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0048","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.spinee.2007.01.011","article-title":"Vertebral osteolysis after posterior interbody lumbar fusion with recombinant human bone morphogenetic protein 2: a report of five cases","volume":"7","author":"Lewandrowski","year":"2007","journal-title":"Spine J"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0049","doi-asserted-by":"crossref","first-page":"E1020","DOI":"10.1097\/BRS.0b013e3182982f8e","article-title":"Complications associated with the use of the recombinant human bone morphogenetic proteins for posterior interbody fusions of the lumbar spine","volume":"38","author":"Chrastil","year":"2013","journal-title":"Spine (Phila Pa 1976)"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0050","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.spinee.2013.08.060","article-title":"Complications with the use of bone morphogenetic protein 2 (BMP-2) in spine surgery","volume":"14","author":"Tannoury","year":"2014","journal-title":"Spine J"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0051","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1007\/s00264-014-2301-6","article-title":"Bone union rate with recombinant human bone morphogenic protein-2 versus autologous iliac bone in PEEK cages for anterior lumbar interbody fusion","volume":"38","author":"Flouzat-Lachaniette","year":"2014","journal-title":"Int Orthop"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0052","doi-asserted-by":"crossref","first-page":"16766","DOI":"10.1073\/pnas.1416121111","article-title":"TSG-6 as a biomarker to predict efficacy of human mesenchymal stem\/progenitor cells (hMSCs) in modulating sterile inflammation in vivo","volume":"111","author":"Lee","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0053","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.stem.2018.05.004","article-title":"Mesenchymal stromal cells: clinical challenges and therapeutic opportunities","volume":"22","author":"Galipeau","year":"2018","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0054","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1002\/sctm.19-0406","article-title":"Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors","volume":"9","author":"Borys","year":"2020","journal-title":"Stem Cells Transl Med"},{"issue":"8","key":"10.1016\/j.jcyt.2024.01.009_bib0055","doi-asserted-by":"crossref","first-page":"386","DOI":"10.3390\/bioengineering9080386","article-title":"Characterization of the aeration and hydrodynamics in vertical-wheel bioreactors","volume":"9","author":"Neto","year":"2022","journal-title":"Bioengineering (Basel)"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0056","doi-asserted-by":"crossref","first-page":"553444","DOI":"10.3389\/fcell.2020.553444","article-title":"Scalable production of human mesenchymal stromal cell-derived extracellular vesicles under serum-\/xeno-free conditions in a microcarrier-based bioreactor culture system","volume":"8","author":"de Almeida Fuzeta","year":"2020","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0057","first-page":"732","article-title":"Upscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile","volume":"25","author":"Jeske","year":"2022","journal-title":"Bioact Mat"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0058","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.bej.2016.11.020","article-title":"Process development for expansion of human mesenchymal stromal cells in a 50 L single-use stirred tank bioreactor","volume":"120","author":"Lawson","year":"2017","journal-title":"Biochem Eng J"},{"issue":"3","key":"10.1016\/j.jcyt.2024.01.009_bib0059","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3390\/bioengineering7030073","article-title":"Bioreactor parameters for microcarrier-based human MSC expansion under xeno-free conditions in a vertical-wheel system","volume":"7","author":"Lembong","year":"2020","journal-title":"Bioengineering"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0060","doi-asserted-by":"crossref","first-page":"pe37","DOI":"10.1126\/stke.2942005pe37","article-title":"Adult bone marrow stem\/progenitor cells (MSCs) are preconditioned by microenvironmental \"niches\" in culture: a two-stage hypothesis for regulation of MSC fate","volume":"2005","author":"Gregory","year":"2005","journal-title":"Sci STKE"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0061","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1089\/ten.tea.2010.0085","article-title":"Sox11 is expressed in early progenitor human multipotent stromal cells and decreases with extensive expansion of the cells","volume":"16","author":"Larson","year":"2010","journal-title":"Tissue Eng Part A"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0062","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1634\/stemcells.20-6-530","article-title":"J.Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality","volume":"20","author":"Sekiya","year":"2002","journal-title":"Stem Cells"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0063","article-title":"Bone substitutes: a review of their characteristics, clinical use, and perspectives for large bone defects management","volume":"9","author":"de Grado","year":"2018","journal-title":"J Tissue Eng"},{"issue":"6","key":"10.1016\/j.jcyt.2024.01.009_bib0064","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.jhsa.2018.10.032","article-title":"Bone graft substitutes: current concepts and future expectations","volume":"44","author":"Lobb","year":"2019","journal-title":"J Hand Surg"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0065","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.bjps.2012.08.015","article-title":"Alveolar bone healing accompanied by severe swelling in cleft children treated with bone morphogenetic protein-2 delivered by hydrogel","volume":"66","author":"Neovius","year":"2013","journal-title":"J Plast Reconstr Aesthet Surg"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0066","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1097\/01.brs.0000245863.52371.c2","article-title":"Increased swelling complications associated with off-label usage of rhBMP-2 in the anterior cervical spine","volume":"31","author":"Smucker","year":"2006","journal-title":"Spine (Phila Pa 1976)"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0067","doi-asserted-by":"crossref","first-page":"534","DOI":"10.21037\/jss.2018.06.19","article-title":"Early term effects of rhBMP-2 on pedicle screw fixation in a sheep model: histomorphometric and biomechanical analyses","volume":"4","author":"Toth","year":"2018","journal-title":"J Spine Surg"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0068","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s00264-004-0579-5","article-title":"Cadaveric allograft microbiology","volume":"28","author":"Ibrahim","year":"2004","journal-title":"Int Orthop"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0069","doi-asserted-by":"crossref","first-page":"3225","DOI":"10.1007\/s11033-019-04782-y","article-title":"The effect of organic solvents on selected microorganisms and model liposome membrane","volume":"46","author":"Dyrda","year":"2019","journal-title":"Mol Biol Rep"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0070","first-page":"623","article-title":"Adaptation of the bacterial action of chloroform to the preparation of bacterins","volume":"34","author":"Bunyea","year":"1927","journal-title":"J Agric Res"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0071","doi-asserted-by":"crossref","first-page":"146293","DOI":"10.1016\/j.brainres.2019.06.012","article-title":"Mesenchymal stem cells derived from induced pluripotent stem cells play a key role in immunomodulation during cardiopulmonary resuscitation","volume":"1720","author":"Yu","year":"2019","journal-title":"Brain Res"},{"issue":"9","key":"10.1016\/j.jcyt.2024.01.009_bib0072","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.3390\/biomedicines10092281","article-title":"Recent advances in extracellular vesicle-based therapies using induced pluripotent stem cell-derived mesenchymal stromal cells","volume":"10","author":"Bertolino","year":"2022","journal-title":"Biomedicines"},{"issue":"7","key":"10.1016\/j.jcyt.2024.01.009_bib0073","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1016\/j.ymthe.2020.04.020","article-title":"Comprehensive molecular profiles of functionally effective MSC-derived extracellular vesicles in immunomodulation","volume":"28","author":"Kim","year":"2020","journal-title":"Mol Ther"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0074","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/nprot.2016.037","article-title":"Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms","volume":"11","author":"Loessner","year":"2016","journal-title":"Nat Protoc"},{"issue":"12","key":"10.1016\/j.jcyt.2024.01.009_bib0075","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1002\/sctm.21-0151","article-title":"A scalable system for generation of mesenchymal stem cells derived from induced pluripotent cells employing bioreactors and degradable microcarriers","volume":"10","author":"Rogers","year":"2021","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.jcyt.2024.01.009_bib0076","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1002\/bit.25187","article-title":"A xenogeneic-free bioreactor system for the clinical-scale expansion of human mesenchymal stem\/stromal cells","volume":"111","author":"Dos Santos","year":"2014","journal-title":"Biotechnol Bioeng"}],"container-title":["Cytotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324924000276?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324924000276?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T05:36:28Z","timestamp":1712208988000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1465324924000276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":76,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["S1465324924000276"],"URL":"https:\/\/doi.org\/10.1016\/j.jcyt.2024.01.009","relation":{},"ISSN":["1465-3249"],"issn-type":[{"value":"1465-3249","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Scalable manufacture of therapeutic mesenchymal stromal cell products on customizable microcarriers in vertical wheel bioreactors that improve direct visualization, product harvest, and cost","name":"articletitle","label":"Article Title"},{"value":"Cytotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcyt.2024.01.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 International Society for Cell & Gene Therapy. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}