{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T03:11:32Z","timestamp":1778814692459,"version":"3.51.4"},"reference-count":25,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004352","name":"Suranaree University of Technology","doi-asserted-by":"publisher","award":["47\/2564"],"award-info":[{"award-number":["47\/2564"]}],"id":[{"id":"10.13039\/501100004352","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["celltherapyjournal.org","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cytotherapy"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.jcyt.2025.02.006","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T11:32:20Z","timestamp":1740396740000},"page":"782-789","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Efficient large-scale expansion of cord blood-derived NK cells: leveraging lipopolysaccharide for enhanced NK cell production"],"prefix":"10.1016","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0545-3901","authenticated-orcid":false,"given":"Hataiwan","family":"Kunkanjanawan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6156-5819","authenticated-orcid":false,"given":"Sirilak","family":"Somredngan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanut","family":"Kunkanjanawan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6070-1843","authenticated-orcid":false,"given":"Patompon","family":"Wongtrakoongate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wannida","family":"Wongsakmanee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veerapol","family":"Khemarangsan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-ichi","family":"Masuyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4764-9101","authenticated-orcid":false,"given":"Rangsun","family":"Parnpai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"21","key":"10.1016\/j.jcyt.2025.02.006_bib0001","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1182\/blood-2009-05-222190","article-title":"NK cells mediate reduction of GVHD by inhibiting activated, alloreactive T cells while retaining GVT effects","volume":"115","author":"Olson","year":"2010","journal-title":"Blood"},{"issue":"4","key":"10.1016\/j.jcyt.2025.02.006_bib0002","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.bbmt.2015.12.028","article-title":"Phase II study of haploidentical natural killer cell infusion for treatment of relapsed or persistent myeloid malignancies following allogeneic hematopoietic cell transplantation","volume":"22","author":"Shaffer","year":"2016","journal-title":"Biol Blood Marrow Transplant"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0003","article-title":"Current perspectives on \u201coff-the-shelf\u201d allogeneic NK and CAR-NK cell therapies","volume":"12","author":"Heipertz","year":"2021","journal-title":"Front Immunol"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0004","first-page":"93","article-title":"Cord blood as a source of natural killer cells","volume":"2","author":"Mehta","year":"2016","journal-title":"Front Med"},{"issue":"3","key":"10.1016\/j.jcyt.2025.02.006_bib0005","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.humimm.2011.12.015","article-title":"The unique profile of cord blood natural killer cells balances incomplete maturation and effective killing function upon activation","volume":"73","author":"Luevano","year":"2012","journal-title":"Hum Immunol"},{"issue":"7","key":"10.1016\/j.jcyt.2025.02.006_bib0006","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1097\/CJI.0b013e3181e475e9","article-title":"Cord blood natural killer cells exhibit impaired lytic immunological synapse formation that is reversed with IL-2 exvivo expansion","volume":"33","author":"Xing","year":"2010","journal-title":"J Immunotherapy"},{"issue":"2","key":"10.1016\/j.jcyt.2025.02.006_bib0007","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009221","article-title":"High log-scale expansion of functional human natural killer cells from umbilical cord blood CD34-positive cells for adoptive cancer immunotherapy","volume":"5","author":"Spanholtz","year":"2010","journal-title":"PLOS ONE"},{"issue":"5","key":"10.1016\/j.jcyt.2025.02.006_bib0008","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/leu.2011.345","article-title":"Expansion of NK cells from cord blood with antileukemic activity using GMP-compliant substances without feeder cells","volume":"26","author":"Tanaka","year":"2012","journal-title":"Leukemia"},{"issue":"4","key":"10.1016\/j.jcyt.2025.02.006_bib0009","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1007\/s13577-018-0213-2","article-title":"Zoledronate increases enrichment, activation and expansion of natural killer cells from umbilical cord blood","volume":"31","author":"Ma","year":"2018","journal-title":"Human Cell"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0010","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s12896-019-0564-0","article-title":"A simple method for in vitro preparation of natural killer cells from cord blood","volume":"19","author":"Mu","year":"2019","journal-title":"BMC Biotechnol"},{"issue":"2","key":"10.1016\/j.jcyt.2025.02.006_bib0011","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.bbmt.2008.11.031","article-title":"Activated notch supports development of cytokine producing NK cells which are hyporesponsive and fail to acquire NK cell effector functions","volume":"15","author":"Bachanova","year":"2009","journal-title":"Biol Blood Marrow Transplant"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0012","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.imlet.2006.07.005","article-title":"The potential involvement of Notch signaling in NK cell development","volume":"107","author":"Rolink","year":"2006","journal-title":"Immunol Lett"},{"issue":"8","key":"10.1016\/j.jcyt.2025.02.006_bib0013","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1002\/stem.2843","article-title":"A novel 2-phenylamino-quinazoline-based compound expands the neural stem cell pool and promotes the hippocampal neurogenesis and the cognitive ability of adult mice","volume":"36","author":"Lu","year":"2018","journal-title":"Stem Cells"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0014","doi-asserted-by":"crossref","first-page":"139","DOI":"10.4049\/jimmunol.165.1.139","article-title":"Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10","volume":"165","author":"Goodier","year":"2000","journal-title":"J Immunol"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0015","first-page":"11","article-title":"Lipopolysaccharide induces IFN-\u03b3 production in human NK cells","volume":"4","author":"Kanevskiy","year":"2013","journal-title":"Front Immunol"},{"issue":"4","key":"10.1016\/j.jcyt.2025.02.006_bib0016","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.jcyt.2025.02.006_bib0017","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.omtm.2020.12.010","article-title":"Human-specific GAPDH qRT-PCR is an accurate and sensitive method of xenograft metastasis quantification","volume":"20","author":"Dahn","year":"2021","journal-title":"Mol Ther Methods Clin Dev"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0018","doi-asserted-by":"crossref","first-page":"139","DOI":"10.4049\/jimmunol.165.1.139","article-title":"Lipopolysaccharide stimulates the proliferation of human CD56+CD3\u2212 NK cells: a regulatory role of monocytes and IL-101","volume":"165","author":"Goodier","year":"2000","journal-title":"J Immunol"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0019","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.3389\/fimmu.2018.01869","article-title":"Natural killer cells: development, maturation, and clinical utilization","volume":"9","author":"Abel","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.jcyt.2025.02.006_bib0020","article-title":"Manufacturing of natural killer cells for treating solid malignancies","volume":"10(1)","author":"Temples","year":"2020","journal-title":"J Immunol Regen Med"},{"issue":"7","key":"10.1016\/j.jcyt.2025.02.006_bib0021","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.jcyt.2020.12.002","article-title":"Generating natural killer cells for adoptive transfer: expanding horizons","volume":"23","author":"Kundu","year":"2021","journal-title":"Cytotherapy"},{"issue":"7","key":"10.1016\/j.jcyt.2025.02.006_bib0022","doi-asserted-by":"crossref","first-page":"3344","DOI":"10.4049\/jimmunol.1400534","article-title":"Notch signaling at later stages of NK cell development enhances KIR expression and functional maturation","volume":"193","author":"Felices","year":"2014","journal-title":"J Immunol"},{"issue":"7","key":"10.1016\/j.jcyt.2025.02.006_bib0023","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1002\/stem.94","article-title":"Notch increases T\/NK potential of human hematopoietic progenitors and inhibits B cell differentiation at a pro-B stage","volume":"27","author":"Benne","year":"2009","journal-title":"Stem Cells"},{"issue":"1","key":"10.1016\/j.jcyt.2025.02.006_bib0024","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.3389\/fphar.2019.01396","article-title":"Antiproliferative and immunoregulatory effects of azelaic acid against acute myeloid leukemia via the activation of notch signaling pathway","volume":"10","author":"Dongdong","year":"2019","journal-title":"Front Pharmacol"},{"issue":"6","key":"10.1016\/j.jcyt.2025.02.006_bib0025","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1586\/14760584.2015.1026808","article-title":"Biosynthetically engineered lipopolysaccharide as vaccine adjuvant","volume":"14","author":"Zariri","year":"2015","journal-title":"Expert Rev Vaccines"}],"container-title":["Cytotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324925000659?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1465324925000659?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T02:43:09Z","timestamp":1778812989000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1465324925000659"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["S1465324925000659"],"URL":"https:\/\/doi.org\/10.1016\/j.jcyt.2025.02.006","relation":{},"ISSN":["1465-3249"],"issn-type":[{"value":"1465-3249","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Efficient large-scale expansion of cord blood-derived NK cells: leveraging lipopolysaccharide for enhanced NK cell production","name":"articletitle","label":"Article Title"},{"value":"Cytotherapy","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jcyt.2025.02.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}