{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:00:58Z","timestamp":1792173658433,"version":"4.4.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:00:00Z","timestamp":1750377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Dentistry"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.jdent.2025.105927","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T11:41:58Z","timestamp":1750851718000},"page":"105927","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Development and characterisation of a novel antimicrobial peptide GA-C16G2 targeting Streptococcus mutans"],"prefix":"10.1016","volume":"161","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6060-8945","authenticated-orcid":false,"given":"Yipeng","family":"Fu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5398-2066","authenticated-orcid":false,"given":"Richard D.","family":"Cannon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5411-3065","authenticated-orcid":false,"given":"Kai Chun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9156-3727","authenticated-orcid":false,"given":"Manikandan","family":"Ekambaram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1305-7287","authenticated-orcid":false,"given":"Paul R.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7745-257X","authenticated-orcid":false,"given":"May L.","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"10482","key":"10.1016\/j.jdent.2025.105927_bib0001","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/S0140-6736(24)02811-3","article-title":"Oral Disorders Collaborators, Trends in the global, regional, and national burden of oral conditions from 1990 to 2021: a systematic analysis for the Global Burden of Disease Study 2021","volume":"405","year":"2025","journal-title":"Lancet"},{"issue":"9555","key":"10.1016\/j.jdent.2025.105927_bib0002","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0140-6736(07)60031-2","article-title":"Dental caries","volume":"369","author":"Selwitz","year":"2007","journal-title":"Lancet"},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0003","doi-asserted-by":"crossref","DOI":"10.1038\/nrdp.2017.30","article-title":"Dental caries","volume":"3","author":"Pitts","year":"2017","journal-title":"Nat. Rev. Dis. Primers."},{"issue":"5","key":"10.1016\/j.jdent.2025.105927_bib0004","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1159\/000330510","article-title":"Clinical efficacy of a specifically targeted antimicrobial peptide mouth rinse: targeted elimination of Streptococcus mutans and prevention of demineralization","volume":"45","author":"Sullivan","year":"2011","journal-title":"Caries. Res."},{"issue":"4","key":"10.1016\/j.jdent.2025.105927_bib0005","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1128\/mr.50.4.353-380.1986","article-title":"Role of Streptococcus mutans in human dental decay","volume":"50","author":"Loesche","year":"1986","journal-title":"Microbiol. Rev."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0006","doi-asserted-by":"crossref","first-page":"5254","DOI":"10.1038\/s41598-020-61912-4","article-title":"Effects of Chlorhexidine mouthwash on the oral microbiome","volume":"10","author":"Bescos","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jdent.2025.105927_bib0007","doi-asserted-by":"crossref","first-page":"587","DOI":"10.3389\/fmicb.2019.00587","article-title":"Resistance Toward Chlorhexidine in Oral Bacteria - Is There Cause for Concern?","volume":"10","author":"Cieplik","year":"2019","journal-title":"Front. Microbiol."},{"issue":"2","key":"10.1016\/j.jdent.2025.105927_bib0008","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1177\/0022034512453725","article-title":"Targeted antimicrobial treatment to re-establish a healthy microbial flora for long-term protection","volume":"24","author":"Eckert","year":"2012","journal-title":"Adv. Dent. Res."},{"key":"10.1016\/j.jdent.2025.105927_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.drup.2023.100954","article-title":"Antimicrobial peptides for combating drug-resistant bacterial infections","volume":"68","author":"Xuan","year":"2023","journal-title":"Drug. Resist. Updates."},{"key":"10.1016\/j.jdent.2025.105927_bib0010","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.drup.2016.04.002","article-title":"Mechanisms and consequences of bacterial resistance to antimicrobial peptides","volume":"26","author":"Andersson","year":"2016","journal-title":"Drug. Resist. Updates."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0011","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nrd3591","article-title":"Designing antimicrobial peptides: form follows function","volume":"11","author":"Fjell","year":"2011","journal-title":"Nat Rev Drug Discov"},{"issue":"9","key":"10.1016\/j.jdent.2025.105927_bib0012","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.tibtech.2011.05.001","article-title":"The expanding scope of antimicrobial peptide structures and their modes of action","volume":"29","author":"Nguyen","year":"2011","journal-title":"Trends. Biotechnol."},{"key":"10.1016\/j.jdent.2025.105927_bib0013","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.cis.2017.06.001","article-title":"The interaction of antimicrobial peptides with membranes","volume":"247","author":"Travkova","year":"2017","journal-title":"Adv. Colloid. Interface. Sci."},{"issue":"11","key":"10.1016\/j.jdent.2025.105927_bib0014","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1128\/AAC.00622-06","article-title":"Targeted killing of Streptococcus mutans by a pheromone-guided \"smart\" antimicrobial peptide","volume":"50","author":"Eckert","year":"2006","journal-title":"Antimicrob. Agents. Chemother."},{"issue":"11","key":"10.1016\/j.jdent.2025.105927_bib0015","doi-asserted-by":"crossref","DOI":"10.3390\/pharmaceutics15112560","article-title":"Development of a novel peptide with antimicrobial and mineralising properties for caries management","volume":"15","author":"Zhang","year":"2023","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.jdent.2025.105927_bib0016","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.micpath.2016.08.004","article-title":"Antimicrobial and enhancement of the antibiotic activity by phenolic compounds: gallic acid, caffeic acid and pyrogallol","volume":"99","author":"Lima","year":"2016","journal-title":"Microb. Pathog."},{"issue":"8","key":"10.1016\/j.jdent.2025.105927_bib0017","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1002\/adhm.201500878","article-title":"Tunicate-inspired gallic acid\/metal ion complex for instant and efficient treatment of dentin hypersensitivity","volume":"5","author":"Prajatelistia","year":"2016","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.jdent.2025.105927_bib0023","doi-asserted-by":"crossref","DOI":"10.1016\/j.jdent.2021.103729","article-title":"A novel dual-action antimicrobial peptide for caries management","volume":"111","author":"Niu","year":"2021","journal-title":"J. Dent."},{"key":"10.1016\/j.jdent.2025.105927_bib0018","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.archoralbio.2017.03.017","article-title":"De novo synthetic short antimicrobial peptides against cariogenic bacteria","volume":"80","author":"Wang","year":"2017","journal-title":"Arch. Oral. Biol."},{"key":"10.1016\/j.jdent.2025.105927_bib0019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vascn.2014.11.003","article-title":"Use of the viability reagent PrestoBlue in comparison with alamarBlue and MTT to assess the viability of human corneal epithelial cells","volume":"71","author":"Xu","year":"2015","journal-title":"J. Pharmacol. Toxicol. Methods."},{"issue":"6","key":"10.1016\/j.jdent.2025.105927_bib0020","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1093\/jac\/dkl120","article-title":"Effect of MUC7 peptides on the growth of bacteria and on Streptococcus mutans biofilm","volume":"57","author":"Wei","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0021","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1159\/000442898","article-title":"Activity of synthetic antimicrobial peptide GH12 against oral streptococci","volume":"50","author":"Tu","year":"2016","journal-title":"Caries. Res."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0022","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1747-0285.2009.00904.x","article-title":"Design and characterization of an acid-activated antimicrobial peptide","volume":"75","author":"Li","year":"2010","journal-title":"Chem. Biol. Drug. Des."},{"key":"10.1016\/j.jdent.2025.105927_bib0024","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2024.158037","article-title":"Study on bionic mineralization of dentin using gallic acid as a linker for calcium phosphate nanocluster formation and collagen modification","volume":"502","author":"Dong","year":"2024","journal-title":"Chem. Eng. J."},{"issue":"7","key":"10.1016\/j.jdent.2025.105927_bib0025","doi-asserted-by":"crossref","first-page":"3446","DOI":"10.1128\/AAC.00342-11","article-title":"Selective membrane disruption: mode of action of C16G2, a specifically targeted antimicrobial peptide","volume":"55","author":"Kaplan","year":"2011","journal-title":"Antimicrob. Agents. Chemother."},{"issue":"11","key":"10.1016\/j.jdent.2025.105927_bib0026","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1021\/jp9911189","article-title":"Solvent Effects on the Secondary Structures of Proteins","volume":"104","author":"Park","year":"2000","journal-title":"J. Phys. Chem. A."},{"issue":"19","key":"10.1016\/j.jdent.2025.105927_bib0027","doi-asserted-by":"crossref","first-page":"12179","DOI":"10.1073\/pnas.182199699","article-title":"Mechanism by which 2,2,2-trifluoroethanol\/water mixtures stabilize secondary-structure formation in peptides: a molecular dynamics study","volume":"99","author":"Roccatano","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"5","key":"10.1016\/j.jdent.2025.105927_bib0028","doi-asserted-by":"crossref","first-page":"425","DOI":"10.2174\/1389203720666190214152439","article-title":"About TFE: old and new findings","volume":"20","author":"Vincenzi","year":"2019","journal-title":"Curr. Protein. Pept. Sci."},{"key":"10.1016\/j.jdent.2025.105927_bib0029","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2022.105407","article-title":"An evaluation of a new high-sensitivity PrestoBlue assay for measuring cell viability and drug cytotoxicity using EA.hy926 endothelial cells","volume":"83","author":"Luzak","year":"2022","journal-title":"Toxicol. Vitro."},{"issue":"6","key":"10.1016\/j.jdent.2025.105927_bib0030","first-page":"807","article-title":"Artificial saliva and its use in biological experiments","volume":"68","author":"Pytko-Polonczyk. J","year":"2017","journal-title":"J. Physiol. Pharmacol."},{"issue":"11","key":"10.1016\/j.jdent.2025.105927_bib0031","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1128\/JCM.43.11.5721-5732.2005","article-title":"Defining the Normal Bacterial Flora of the Oral Cavity","volume":"43","author":"Aas","year":"2005","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0032","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1186\/s13568-022-01499-3","article-title":"Streptococcus mutans membrane vesicles inhibit the biofilm formation of Streptococcus gordonii and Streptococcus sanguinis","volume":"12","author":"Cui","year":"2022","journal-title":"AMB. Express."},{"issue":"13","key":"10.1016\/j.jdent.2025.105927_bib0033","doi-asserted-by":"crossref","first-page":"4632","DOI":"10.1128\/JB.00276-08","article-title":"Streptococcal antagonism in oral biofilms: streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans","volume":"190","author":"Kreth","year":"2008","journal-title":"J. Bacteriol."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0034","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1128\/AEM.71.1.354-362.2005","article-title":"Interactions between oral bacteria: inhibition of Streptococcus mutans bacteriocin production by Streptococcus gordonii","volume":"71","author":"Wang","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"issue":"24","key":"10.1016\/j.jdent.2025.105927_bib0035","doi-asserted-by":"crossref","first-page":"7569","DOI":"10.1073\/pnas.1506207112","article-title":"Precision-guided antimicrobial peptide as a targeted modulator of human microbial ecology","volume":"112","author":"Guo","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"10","key":"10.1016\/j.jdent.2025.105927_bib0036","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1002\/j.0022-0337.2001.65.10.tb03446.x","article-title":"The microbiology of primary dental caries in humans","volume":"65","author":"Tanzer","year":"2001","journal-title":"J. Dent. Educ."},{"issue":"4","key":"10.1016\/j.jdent.2025.105927_bib0037","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1111\/j.1365-2958.2009.06693.x","article-title":"Peptide alarmone signalling triggers an auto-active bacteriocin necessary for genetic competence","volume":"72","author":"Perry","year":"2009","journal-title":"Mol. Microbiol."},{"issue":"5","key":"10.1016\/j.jdent.2025.105927_bib0038","doi-asserted-by":"crossref","first-page":"435","DOI":"10.4161\/viru.2.5.16140","article-title":"Bacterial interactions in dental biofilm","volume":"2","author":"Huang","year":"2011","journal-title":"Virulence"},{"key":"10.1016\/j.jdent.2025.105927_bib0039","doi-asserted-by":"crossref","DOI":"10.3402\/jom.v6.26189","article-title":"Comparing the cariogenic species Streptococcus sobrinus and S. mutans on whole genome level","volume":"6","author":"Conrads","year":"2014","journal-title":"J. Oral. Microbiol."},{"key":"10.1016\/j.jdent.2025.105927_bib0040","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.micpath.2016.08.004","article-title":"Antimicrobial and enhancement of the antibiotic activity by phenolic compounds: gallic acid, caffeic acid and pyrogallol","volume":"99","author":"Lima","year":"2016","journal-title":"Microb. Pathog."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0041","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.lwt.2013.11.037","article-title":"The antimicrobial, mechanical, physical and structural properties of chitosan\u2013gallic acid films","volume":"57","author":"Sun","year":"2014","journal-title":"LWT-Food. Sci. Technol."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0042","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jdsr.2017.08.002","article-title":"Role of Streptococcus mutans surface proteins for biofilm formation","volume":"54","author":"Matsumoto-Nakano","year":"2018","journal-title":"Jpn. Dent. Sci. Rev."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0043","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1124\/pr.55.1.2","article-title":"Mechanisms of antimicrobial peptide action and resistance","volume":"55","author":"Yeaman","year":"2003","journal-title":"Pharmacol. Rev."},{"issue":"1","key":"10.1016\/j.jdent.2025.105927_bib0044","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nrd3591","article-title":"Designing antimicrobial peptides: form follows function","volume":"11","author":"Fjell","year":"2012","journal-title":"Nat. Rev. Drug. Discov."},{"issue":"3","key":"10.1016\/j.jdent.2025.105927_bib0045","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/omi.12333","article-title":"The multifaceted roles of antimicrobial peptides in oral diseases","volume":"36","author":"Niu","year":"2021","journal-title":"Mol. Oral. Microbiol."},{"issue":"3","key":"10.1016\/j.jdent.2025.105927_bib0046","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1177\/0022034516679973","article-title":"Antimicrobial peptides: mechanisms of action and resistance","volume":"96","author":"Bechinger","year":"2017","journal-title":"J. Dent. Res."}],"container-title":["Journal of Dentistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300571225003719?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0300571225003719?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T23:39:42Z","timestamp":1765841982000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0300571225003719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":46,"alternative-id":["S0300571225003719"],"URL":"https:\/\/doi.org\/10.1016\/j.jdent.2025.105927","relation":{},"ISSN":["0300-5712"],"issn-type":[{"value":"0300-5712","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and characterisation of a novel antimicrobial peptide GA-C16G2 targeting Streptococcus mutans","name":"articletitle","label":"Article Title"},{"value":"Journal of Dentistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jdent.2025.105927","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"105927"}}