{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:28:47Z","timestamp":1770881327948,"version":"3.50.1"},"reference-count":34,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Maruho-Takagi Dermatology Foundation","award":["19K08753"],"award-info":[{"award-number":["19K08753"]}]},{"name":"Lydia O\u2019leary Memorial Pias Dermatological Foundation"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jdsjournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Dermatological Science"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.jdermsci.2020.07.006","type":"journal-article","created":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T11:46:35Z","timestamp":1596627995000},"page":"185-192","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Novel ex vivo disease model for extramammary Paget\u2019s disease using the cancer tissue-originated spheroid method"],"prefix":"10.1016","volume":"99","author":[{"given":"Takahiro","family":"Arita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jumpei","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miho","family":"Tsutsumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai","family":"Kanemaru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Matsui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiyasu","family":"Arakawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norito","family":"Katoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Asai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jdermsci.2020.07.006_bib0005","first-page":"187","article-title":"Paget\u2019s disease affecting the scrotum and penis","volume":"40","author":"Crocker","year":"1889","journal-title":"Trans. Pathol. Soc. Lond."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0010","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1097\/00000372-199604000-00003","article-title":"Gross cystic disease fluid protein-15 reactivity in extramammary Paget\u2019s disease with and without associated internal malignancy","volume":"18","author":"Kohler","year":"1996","journal-title":"Am. J. Dermatopathol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0015","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1136\/jcp.53.10.742","article-title":"Mammary and extramammary Paget\u2019s disease","volume":"53","author":"Lloyd","year":"2000","journal-title":"J. Clin. Pathol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0020","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1046\/j.1365-2133.2000.03291.x","article-title":"The use of cytokeratins 7 and 20 in the diagnosis of primary and secondary extramammary Paget\u2019s disease","volume":"142","author":"Ohnishi","year":"2000","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0025","first-page":"313","article-title":"Extramammary Paget\u2019s disease: treatment, prognostic factors and outcome in 76 patients","volume":"158","author":"Hatta","year":"2008","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0030","doi-asserted-by":"crossref","first-page":"1938","DOI":"10.1111\/j.1524-4725.2012.02584.x","article-title":"Prognostic indicators in 35 patients with extramammary Paget\u2019s disease","volume":"38","author":"Ito","year":"2012","journal-title":"Dermatol. Surg."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0035","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1186\/s12885-018-4257-1","article-title":"Survival analysis of patients with invasive extramammary Paget disease: implications of anatomic sites","volume":"18","author":"Yao","year":"2018","journal-title":"BMC Cancer"},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0040","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/j.1365-2133.2005.07046.x","article-title":"Metastatic extramammary Paget\u2019s disease successfully controlled with tumour dormancy therapy using docetaxel","volume":"154","author":"Fujisawa","year":"2006","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0045","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1111\/bjd.12788","article-title":"Combination chemotherapy for metastatic extramammary Paget disease","volume":"170","author":"Oashi","year":"2014","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0050","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s10147-014-0686-2","article-title":"Combination chemotherapy of low-dose 5-fluorouracil and cisplatin for advanced extramammary Paget\u2019s disease","volume":"20","author":"Tokuda","year":"2015","journal-title":"Int. J. Clin. Oncol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0055","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1346-8138.1999.tb03477.x","article-title":"A case of metastatic extramammary Paget\u2019s disease that responded to combination chemotherapy","volume":"26","author":"Yamazaki","year":"1999","journal-title":"J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0060","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1111\/j.1346-8138.2010.01149.x","article-title":"Successful treatment of metastatic extramammary Paget\u2019s disease with S-1 and docetaxel combination chemotherapy","volume":"38","author":"Matsushita","year":"2011","journal-title":"J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0065","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1111\/1346-8138.15255","article-title":"Weekly docetaxel monotherapy for metastatic extramammary Paget\u2019s disease: retrospective single-institute analysis","volume":"40","author":"Nakamura","year":"2020","journal-title":"J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0070","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/1346-8138.13200","article-title":"Usefulness of docetaxel as first-line chemotherapy for metastatic extramammary","volume":"43","author":"Yoshino","year":"2016","journal-title":"J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0075","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1111\/bjd.17922","article-title":"Single-agent taxane is useful in palliative chemotherapy for advanced extramammary Paget disease: a case series","volume":"181","author":"Kato","year":"2019","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0080","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1046\/j.1365-4362.2001.01026.x","article-title":"Overexpression of transforming growth factor-beta3 immunohistochemical staining in extramammary Paget\u2019s disease, but downregulated expression in Bowen\u2019s disease","volume":"40","author":"Kawakami","year":"2001","journal-title":"Int. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0085","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1111\/j.1365-2133.2006.07366.x","article-title":"Expression of insulin-like growth factor-1 receptor, p-AKT and p-ERK1\/2 protein in extramammary Paget\u2019s disease","volume":"155","author":"Liu","year":"2006","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0090","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1111\/j.1600-0560.2010.01563.x","article-title":"Nerve growth factor, brain-derived neurotrophic factor and their high-affinity receptors are overexpressed in extramammary Paget\u2019s disease","volume":"37","author":"Qian","year":"2010","journal-title":"J. Cutan. Pathol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0095","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1111\/bjd.12249","article-title":"Human epidermal growth factor receptor 2 protein overexpression and gene amplification in extramammary Paget disease","volume":"168","author":"Tanaka","year":"2013","journal-title":"Br. J. Dermatol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0100","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jdermsci.2017.03.012","article-title":"Podoplanin expression in peritumoral keratinocytes predicts aggressive behavior in extramammary Paget\u2019s disease","volume":"87","author":"Cho","year":"2017","journal-title":"J. Dermatol. Sci."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0105","doi-asserted-by":"crossref","first-page":"6235","DOI":"10.1073\/pnas.1015938108","article-title":"Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer","volume":"108","author":"Kondo","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0110","doi-asserted-by":"crossref","first-page":"470","DOI":"10.3390\/cells8050470","article-title":"Application of Cancer organoid model for drug screening and personalized therapy","volume":"8","author":"Kondo","year":"2019","journal-title":"Cells"},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0115","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1097\/JTO.0b013e3182779ccf","article-title":"Spheroid culture of primary lung cancer cells with neuregulin 1\/HER3 pathway activation","volume":"8","author":"Endo","year":"2013","journal-title":"J. Thorac. Oncol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0120","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.juro.2012.12.106","article-title":"Involvement of heregulin\/HER3 in the primary culture of human urothelial cancer","volume":"190","author":"Okuyama","year":"2013","journal-title":"J. Urol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0125","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1002\/ijc.29349","article-title":"Radiation sensitivity assay with a panel of patient-derived spheroids of small cell carcinoma of the cervix","volume":"136","author":"Nakajima","year":"2015","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0130","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1111\/cas.12898","article-title":"Drug screening and grouping by sensitivity with a panel of primary cultured cancer spheroids derived from endometrial cancer","volume":"107","author":"Kiyohara","year":"2016","journal-title":"Cancer Sci."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0135","doi-asserted-by":"crossref","first-page":"4111","DOI":"10.1182\/blood-2007-03-082586","article-title":"Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling","volume":"110","author":"Wang","year":"2007","journal-title":"Blood"},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0140","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/S0014-4827(02)00101-5","article-title":"The deaf and the dumb: the biology of ErbB-2 and ErbB-3","volume":"284","author":"Citri","year":"2003","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0145","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/cas.12567","article-title":"High-dose chemotherapeutics of intravesical chemotherapy rapidly induce mitochondrial dysfunction in bladder cancer-derived spheroids","volume":"106","author":"Yoshida","year":"2015","journal-title":"Cancer Sci."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0150","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1126\/science.2470152","article-title":"Studies of the HER-2\/neu proto-oncogene in human breast and ovarian cancer","volume":"244","author":"Slamon","year":"1989","journal-title":"Science"},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0155","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1200\/JCO.1993.11.10.1936","article-title":"Clinical significance of HER-2\/neu oncogene amplification in primary breast cancer. The South Australian Breast Cancer Study Group","volume":"11","author":"Seshadri","year":"1993","journal-title":"J. Clin. Oncol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0160","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1016\/j.humpath.2005.09.009","article-title":"Extramammary Paget\u2019s disease: analysis of growth signal pathway from the human epidermal growth factor receptor 2 protein","volume":"36","author":"Ogawa","year":"2005","journal-title":"Hum. Pathol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0165","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.ygyno.2007.12.014","article-title":"HER-2\/neu targeting for recurrent vulvar Paget\u2019s disease A case report and literature review","volume":"111","author":"Karam","year":"2008","journal-title":"Gynecol. Oncol."},{"key":"10.1016\/j.jdermsci.2020.07.006_bib0170","article-title":"Dramatic clinical response of relapsed metastatic extramammary Paget\u2019s disease to trastuzumab monotherapy","volume":"2012","author":"Wakabayashi","year":"2012","journal-title":"Case Rep. Dermatol. Med."}],"container-title":["Journal of Dermatological Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0923181120302371?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0923181120302371?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T04:50:34Z","timestamp":1760331034000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0923181120302371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["S0923181120302371"],"URL":"https:\/\/doi.org\/10.1016\/j.jdermsci.2020.07.006","relation":{},"ISSN":["0923-1811"],"issn-type":[{"value":"0923-1811","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Novel ex vivo disease model for extramammary Paget\u2019s disease using the cancer tissue-originated spheroid method","name":"articletitle","label":"Article Title"},{"value":"Journal of Dermatological Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jdermsci.2020.07.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}