{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T09:49:31Z","timestamp":1780566571386,"version":"3.54.1"},"reference-count":17,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,1,2]],"date-time":"2016-01-02T00:00:00Z","timestamp":1451692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371137"],"award-info":[{"award-number":["81371137"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Presidential Foundation of Nanfang Hospital, Southern Medical University","award":["2014C020"],"award-info":[{"award-number":["2014C020"]}]},{"name":"Scientific Research Staring Foundation of Southern Medical University","award":["PY2014N051"],"award-info":[{"award-number":["PY2014N051"]}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","e-jds.com","clinicalkey.es","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Dental Sciences"],"published-print":{"date-parts":[[2016,3]]},"DOI":"10.1016\/j.jds.2015.08.002","type":"journal-article","created":{"date-parts":[[2015,11,19]],"date-time":"2015-11-19T06:30:41Z","timestamp":1447914641000},"page":"54-58","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["The impact of different DNA extraction methods on the analysis of microbial diversity of oral saliva from healthy youths by polymerase chain reaction-denaturing gradient gel electrophoresis"],"prefix":"10.1016","volume":"11","author":[{"given":"Ming","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bu-Ling","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi-Long","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jds.2015.08.002_bib1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1146\/annurev.mi.39.100185.001541","article-title":"Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats","volume":"39","author":"Staley","year":"1985","journal-title":"Annu Rev Microbiol"},{"key":"10.1016\/j.jds.2015.08.002_bib2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1475-2891-9-23","article-title":"Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens","volume":"9","author":"Ariefdjohan","year":"2010","journal-title":"Nutr J"},{"key":"10.1016\/j.jds.2015.08.002_bib3","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1590\/S1517-83822014000100023","article-title":"Comparison of DNA extraction protocols for microbial communities from soil treated with biochar","volume":"45","author":"Leite","year":"2014","journal-title":"Braz J Microbiol"},{"key":"10.1016\/j.jds.2015.08.002_bib4","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.anaerobe.2013.09.015","article-title":"Effect of DNA extraction and sample preservation method on rumen bacterial population","volume":"29","author":"Fliegerova","year":"2014","journal-title":"Anaerobe"},{"key":"10.1016\/j.jds.2015.08.002_bib5","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1007\/s11274-015-1856-8","article-title":"Comparison of bacterial community structures of terrestrial cyanobacterium Nostoc flagelliforme in three different regions of China using PCR-DGGE analysis","volume":"31","author":"Han","year":"2015","journal-title":"World J Microb Biot"},{"key":"10.1016\/j.jds.2015.08.002_bib6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.ijfoodmicro.2015.03.033","article-title":"PCR-DGGE assessment of the bacterial diversity in Spanish-style green table-olive fermentations","volume":"205","author":"Lucena-Padros","year":"2015","journal-title":"Int J Food Microbiol"},{"key":"10.1016\/j.jds.2015.08.002_bib7","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/nprot.2006.142","article-title":"Isolation of DNA from bacterial samples of the human gastrointestinal tract","volume":"1","author":"Zoetendal","year":"2006","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jds.2015.08.002_bib8","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.mimet.2009.09.012","article-title":"Metagenomic study of the oral microbiota by Illumina high-throughput sequencing","volume":"79","author":"Lazarevic","year":"2009","journal-title":"J Microbiol Meth"},{"key":"10.1016\/j.jds.2015.08.002_bib9","doi-asserted-by":"crossref","first-page":"e88982","DOI":"10.1371\/journal.pone.0088982","article-title":"The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing","volume":"9","author":"Kennedy","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jds.2015.08.002_bib10","doi-asserted-by":"crossref","first-page":"130","DOI":"10.3389\/fmicb.2015.00130","article-title":"Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differences","volume":"6","author":"Wagner Mackenzie","year":"2015","journal-title":"Front Microbiol"},{"key":"10.1016\/j.jds.2015.08.002_bib11","doi-asserted-by":"crossref","first-page":"400","DOI":"10.4238\/2015.January.23.13","article-title":"Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples","volume":"14","author":"Espinosa","year":"2015","journal-title":"Genet Mol Res"},{"key":"10.1016\/j.jds.2015.08.002_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0167-7012(99)00054-8","article-title":"Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis","volume":"38","author":"Gelsomino","year":"1999","journal-title":"J Microbiol Meth"},{"key":"10.1016\/j.jds.2015.08.002_bib13","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.fsigen.2014.12.001","article-title":"A comparison of four methods for PCR inhibitor removal","volume":"16","author":"Hu","year":"2015","journal-title":"Forensic Sci Int Genet"},{"key":"10.1016\/j.jds.2015.08.002_bib14","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1177\/104063870902100603","article-title":"Removal of real-time reverse transcription polymerase chain reaction (RT-PCR) inhibitors associated with cloacal swab samples and tissues for improved diagnosis of Avian influenza virus by RT-PCR","volume":"21","author":"Das","year":"2009","journal-title":"J Vet Diagn Invest"},{"key":"10.1016\/j.jds.2015.08.002_bib15","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.fsigen.2012.06.013","article-title":"PCR inhibitor removal using the NucleoSpin(R) DNA Clean-Up XS kit","volume":"7","author":"Faber","year":"2013","journal-title":"Forensic Sci Int Genet"},{"key":"10.1016\/j.jds.2015.08.002_bib16","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.archoralbio.2005.02.001","article-title":"Variation in bacterial DGGE patterns from human saliva: over time, between individuals and in corresponding dental plaque microcosms","volume":"50","author":"Rasiah","year":"2005","journal-title":"Arch Oral Biol"},{"key":"10.1016\/j.jds.2015.08.002_bib17","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1046\/j.1365-2672.2003.01876.x","article-title":"Growth and molecular characterization of dental plaque microcosms","volume":"94","author":"McBain","year":"2003","journal-title":"J Appl Microbiol"}],"container-title":["Journal of Dental Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1991790215000975?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1991790215000975?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T15:58:24Z","timestamp":1537199904000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1991790215000975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["S1991790215000975"],"URL":"https:\/\/doi.org\/10.1016\/j.jds.2015.08.002","relation":{},"ISSN":["1991-7902"],"issn-type":[{"value":"1991-7902","type":"print"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The impact of different DNA extraction methods on the analysis of microbial diversity of oral saliva from healthy youths by polymerase chain reaction-denaturing gradient gel electrophoresis","name":"articletitle","label":"Article Title"},{"value":"Journal of Dental Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jds.2015.08.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}