{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:02:26Z","timestamp":1789786946213,"version":"4.0.1"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005774","name":"University of Barcelona","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Environmental Management"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.jenvman.2026.130737","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:04:50Z","timestamp":1787047490000},"page":"130737","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A chromogenic medium for the enumeration of Escherichia coli and total coliforms in 100\u202fmL water samples: validation of an MPN-based method according to ISO 9308-2"],"prefix":"10.1016","volume":"415","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7632-6758","authenticated-orcid":false,"given":"Anicet R.","family":"Blanch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0943-4805","authenticated-orcid":false,"given":"Ariadna","family":"Jorba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4429-9094","authenticated-orcid":false,"given":"Julia","family":"Mart\u00edn-D\u00edaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1075-0732","authenticated-orcid":false,"given":"Miriam","family":"Pascual-Benito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4968-7299","authenticated-orcid":false,"given":"Raquel","family":"Casas-Mangas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6301-8362","authenticated-orcid":false,"given":"Helga","family":"N\u00fa\u00f1ez-Chapela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3723-8787","authenticated-orcid":false,"given":"Javier","family":"M\u00e9ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8522-2826","authenticated-orcid":false,"given":"Daniel","family":"Asensio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jenvman.2026.130737_bib1","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1046\/j.1365-2672.1997.00278.x","article-title":"Bacteriophages of enteric bacteria in drinking water, comparison of their distribution in two countries","volume":"83","author":"Armon","year":"1997","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.2166\/wst.1997.0755","article-title":"Evaluation of Escherichia coli as the main indicator of faecal pollution","volume":"35","author":"Baudi\u0161ov\u00e1","year":"1997","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.jenvman.2026.130737_bib3","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1093\/jaoacint\/qsac120","article-title":"Validation of the AquaCHROMTM ECC for the detection and enumeration of coliforms and Escherichia coli in water samples: AOAC performance tested MethodSM 072202","volume":"106","author":"Blackburn","year":"2023","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.jenvman.2026.130737_bib4","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/j.1365-2672.2006.03141.x","article-title":"Characterization of bacterial coliform occurrences in different zones of a drinking water distribution system","volume":"102","author":"Blanch","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib5","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1007\/s00248-008-9430-7","article-title":"Resistance of faecal coliforms and enterococci populations in sludge and biosolids to different hygienisation treatments","volume":"57","author":"Bonjoch","year":"2009","journal-title":"Microb. Ecol."},{"key":"10.1016\/j.jenvman.2026.130737_bib6","doi-asserted-by":"crossref","first-page":"3360","DOI":"10.1128\/AEM.00020-11","article-title":"Validation of alternative methods for the analysis of drinking water and their application to Escherichia coli","volume":"77","author":"Boubetra","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib7","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.ijantimicag.2017.11.014","article-title":"Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates","volume":"51","author":"Brown-Jaque","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.jenvman.2026.130737_bib8","doi-asserted-by":"crossref","DOI":"10.1038\/srep33000","article-title":"Bacteriophages in clinical samples can interfere with microbiological diagnostic tools","volume":"6","author":"Brown-Jaque","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jenvman.2026.130737_bib9","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1093\/jac\/dkt528","article-title":"Quinolone resistance genes (qnrA and qnrS) in bacteriophage particles from wastewater samples and the effect of inducing agents on packaged antibiotic resistance genes","volume":"69","author":"Colomer-Lluch","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.jenvman.2026.130737_bib10","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1093\/jac\/dks477","article-title":"Detection of quinolone-resistant Escherichia coli isolates belonging to clonal groups O25b:H4-B2-ST131 and O25b:H4-D-ST69 in raw sewage and river water in Barcelona, Spain","volume":"68","author":"Colomer-Lluch","year":"2013","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.jenvman.2026.130737_bib11","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1128\/aem.41.5.1152-1158.1981","article-title":"Membrane filter method for enumerating Escherichia coli","volume":"41","author":"Dufour","year":"1981","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib12","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1128\/aem.43.6.1320-1329.1982","article-title":"Fluorogenic assays for immediate confirmation of Escherichia coli","volume":"43","author":"Feng","year":"1982","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib13","article-title":"MPN: Most probable number and other microbial enumeration techniques","author":"Ferguson","year":"2026","journal-title":"R package version 0.5.0"},{"key":"10.1016\/j.jenvman.2026.130737_bib14","first-page":"131","article-title":"Using IDEXX colilert and quantitray for the simultaneous detection and enumeration of total coliforms and E. coli","volume":"89","author":"Fricker","year":"1997","journal-title":"J. Am. Water Works Assoc."},{"key":"10.1016\/j.jenvman.2026.130737_bib15","series-title":"Valytics: Statistical Methods for Analytical Method Comparison and Validation (Manual)","author":"Grassi","year":"2026"},{"key":"10.1016\/j.jenvman.2026.130737_bib16","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0022-2836(83)80284-8","article-title":"Studies on transformation of Escherichia coli with plasmids","volume":"166","author":"Hanahan","year":"1983","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jenvman.2026.130737_bib17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/j.1365-2672.1983.tb02660.x","article-title":"Automated statistical analysis of microbial enumeration by dilution series","volume":"55","author":"Hurley","year":"1983","journal-title":"J. Appl. Bacteriol."},{"key":"10.1016\/j.jenvman.2026.130737_bib18","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1128\/IAI.02916-14","article-title":"BaeSR, involved in envelope stress response, protects against lysogenic conversion by Shiga toxin 2-encoding phages","volume":"83","author":"Imamovic","year":"2015","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jenvman.2026.130737_bib19","doi-asserted-by":"crossref","first-page":"3536","DOI":"10.1128\/AEM.02703-10","article-title":"Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad","volume":"77","author":"Imamovic","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib20","series-title":"ISO 9308-3:1999. Water Quality \u2014 Detection and Enumeration of Escherichia coli and Coliform Bacteria \u2014 Part 3: Miniaturized Method (Most Probable Number) by Inoculation in Liquid Medium","year":"1999"},{"key":"10.1016\/j.jenvman.2026.130737_bib21","series-title":"ISO 9308-2:2012. Water Quality \u2014 Enumeration of Escherichia coli and Coliform Bacteria \u2014 Part 2: Most Probable Number Method","year":"2012"},{"key":"10.1016\/j.jenvman.2026.130737_bib22","series-title":"ISO 9308-1:2014. Water Quality \u2014 Detection and Enumeration of Escherichia coli and Coliform Bacteria \u2014 Part 1: Membrane Filtration Method for Waters with Low Bacterial Background Flora","year":"2014"},{"key":"10.1016\/j.jenvman.2026.130737_bib23","series-title":"ISO 16140-2:2016. Microbiology of the Food Chain \u2014 Method Validation \u2014 Part 2: Protocol for the Validation of Alternative (Proprietary) Methods Against a Reference Method","year":"2016"},{"key":"10.1016\/j.jenvman.2026.130737_bib24","series-title":"ISO 13843:2017. Water Quality \u2014 Requirements for Establishing Performance Characteristics of Quantitative Microbiological Methods","year":"2017"},{"key":"10.1016\/j.jenvman.2026.130737_bib25","first-page":"1660","article-title":"Reconsideration of the derivation of Most probable numbers, their standard deviations, confidence bounds and rarity values","volume":"109","author":"Jarvis","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib26","article-title":"Bacteriophages in bathing waters: a feasibility study on the development of a method based on bacteriophages for the determination of microbiological quality of bathing waters","author":"Jofre","year":"2000"},{"key":"10.1016\/j.jenvman.2026.130737_bib27","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/S1286-4579(02)01637-4","article-title":"Escherichia coli in disguise: molecular origins of shigella","volume":"4","author":"Lan","year":"2002","journal-title":"Microb. Infect."},{"issue":"3","key":"10.1016\/j.jenvman.2026.130737_bib28","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1093\/clinchem\/39.3.424","article-title":"Evaluation of regression procedures for methods comparison studies","volume":"39","author":"Linnet","year":"1993","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jenvman.2026.130737_bib29","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0168-1605(00)00312-3","article-title":"New developments in chromogenic and fluorogenic culture media","volume":"60","author":"Manafi","year":"2000","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0078906","article-title":"Prevalence of antibiotic resistance genes and bacterial community composition in a river influenced by a wastewater treatment plant","volume":"8","author":"Marti","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jenvman.2026.130737_bib31","series-title":"Preparing Exact Most Probable Number (MPN) Tables Using Occupancy Theory, and Accompanying Measures of Uncertainty (No. Technical Report 121)","author":"McBride","year":"2003"},{"key":"10.1016\/j.jenvman.2026.130737_bib32","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1111\/j.1462-2920.2004.00604.x","article-title":"Free Shiga toxin bacteriophages isolated from sewage showed diversity although the stx genes appeared conserved","volume":"6","author":"Muniesa","year":"2004","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib33","doi-asserted-by":"crossref","first-page":"267","DOI":"10.2166\/wh.2007.008b","article-title":"A comparison of ten USEPA approved total coliform\/E. coli tests","volume":"5","author":"Olstadt","year":"2007","journal-title":"J. Water Health"},{"key":"10.1016\/j.jenvman.2026.130737_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2024.177500","article-title":"Efficient quantification of somatic coliphages in water: development and validation of the enumera\u00aerapid kit","volume":"957","author":"Pascual-Benito","year":"2024","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.jenvman.2026.130737_bib35","author":"Potapov"},{"key":"10.1016\/j.jenvman.2026.130737_bib36","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1111\/j.1365-2958.2005.04584.x","article-title":"Antibiotic-induced SOS response promotes horizontal dissemination of pathogenicity island-encoded virulence factors in staphylococci","volume":"56","author":"Ubeda","year":"2005","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jenvman.2026.130737_bib37","series-title":"Summary Progress Update 2021 \u2013 SDG 6 \u2013 Water and Sanitation for all","year":"2021"},{"key":"10.1016\/j.jenvman.2026.130737_bib38","series-title":"Potable reuse: guidance for producing safe drinking-water","year":"2017"},{"key":"10.1016\/j.jenvman.2026.130737_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114594","article-title":"Single Escherichia coli bacteria detection using a chemiluminescence digital microwell array chip","volume":"215","author":"Wu","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.jenvman.2026.130737_bib40","doi-asserted-by":"crossref","first-page":"17853","DOI":"10.1021\/acs.analchem.2c03604","article-title":"All-in-one Escherichia coli viability assay for multi-dimensional detection of uncomplicated urinary tract infections","volume":"94","author":"Wu","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jenvman.2026.130737_bib41","doi-asserted-by":"crossref","first-page":"462","DOI":"10.3390\/mi16040462","article-title":"Recent advances in microfluidics-based monitoring of waterborne pathogens: from isolation to detection","volume":"16","author":"Xu","year":"2025","journal-title":"Micromachines"}],"container-title":["Journal of Environmental Management"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301479726021973?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301479726021973?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:38:37Z","timestamp":1789785517000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0301479726021973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":41,"alternative-id":["S0301479726021973"],"URL":"https:\/\/doi.org\/10.1016\/j.jenvman.2026.130737","relation":{},"ISSN":["0301-4797"],"issn-type":[{"value":"0301-4797","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A chromogenic medium for the enumeration of Escherichia coli and total coliforms in 100\u202fmL water samples: validation of an MPN-based method according to ISO 9308-2","name":"articletitle","label":"Article Title"},{"value":"Journal of Environmental Management","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jenvman.2026.130737","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"130737"}}