{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:28:59Z","timestamp":1787315339119,"version":"build-2736575974"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ethnopharmacology"],"published-print":{"date-parts":[[2017,1]]},"DOI":"10.1016\/j.jep.2016.12.029","type":"journal-article","created":{"date-parts":[[2016,12,16]],"date-time":"2016-12-16T18:35:53Z","timestamp":1481913353000},"page":"160-167","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["An ex vivo evaluation of the efficacy of andrographolide in modulating differential expression of transcription factors and target genes in periodontal cells and its potential role in treating periodontal diseases"],"prefix":"10.1016","volume":"196","author":[{"family":"Ambili R","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasanthila","family":"Janam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.S.","family":"Saneesh Babu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Vinod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.R.","family":"Anil Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.V.","family":"Kumary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Asha Nair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Radhakrishna Pillai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.jep.2016.12.029_bib1","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1007\/s00210-014-1041-x","article-title":"Insights into the antiatherogenic molecular mechanisms of andrographolide against Porphyromonas gingivalis-induced atherosclerosis in rabbits","volume":"387","author":"Al Batran","year":"2014","journal-title":"Naunyn--Schmiedeberg's Arch. Pharmacol."},{"key":"10.1016\/j.jep.2016.12.029_bib2","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/276329","article-title":"In-vivo effect of andrographolide on alveolar bone resorption induced by Porphyromonas gingivalis and its relation with antioxidant enzymes","author":"Al Batran","year":"2013","journal-title":"BioMed. Res. Int."},{"issue":"7","key":"10.1016\/j.jep.2016.12.029_bib3","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1902\/jop.2005.76.7.1148","article-title":"Expression of activated transcription factor nuclear factor-\u03baB in periodontally diseased tissues","volume":"76","author":"Ambili","year":"2005","journal-title":"J. Periodontol."},{"issue":"1","key":"10.1016\/j.jep.2016.12.029_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1902\/annals.1999.4.1.1","article-title":"Development of a classification system for periodontal diseases and conditions","volume":"4","author":"Armitage","year":"1999","journal-title":"Ann. Periodontol."},{"issue":"2","key":"10.1016\/j.jep.2016.12.029_bib5","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1517\/13543776.2012.661718","article-title":"Modifications of andrographolide to increase some biological activities: a patent review (2006\u20132011)","volume":"22","author":"Aromdee","year":"2012","journal-title":"Expert Opin. Ther. Pat."},{"issue":"1","key":"10.1016\/j.jep.2016.12.029_bib6","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1600-0757.2005.00140.x","article-title":"Molecular and cell biology of healthy and diseased periodontal tissues","volume":"40","author":"Bartold","year":"2006","journal-title":"Periodontology 2000"},{"issue":"2","key":"10.1016\/j.jep.2016.12.029_bib7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.atc.2006.01.001","article-title":"Linking stress to inflammation","volume":"24","author":"Bierhaus","year":"2006","journal-title":"Anesthesiol. Clin. North Am."},{"issue":"12","key":"10.1016\/j.jep.2016.12.029_bib8","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1038\/embor.2009.243","article-title":"IKK\/NF\u2010\u03baB and STAT3 pathways: central signaling hubs in inflammation\u2010mediated tumour promotion and metastasis","volume":"10","author":"Bollrath","year":"2009","journal-title":"EMBO Rep."},{"issue":"8","key":"10.1016\/j.jep.2016.12.029_bib9","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1007\/s10067-009-1180-5","article-title":"Efficacy of an Andrographis paniculata composition for the relief of rheumatoid arthritis symptoms: a prospective randomized placebo-controlled trial","volume":"28","author":"Burgos","year":"2009","journal-title":"Clin. Rheumatol."},{"issue":"5","key":"10.1016\/j.jep.2016.12.029_bib10","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/1099-1573(200008)14:5<333::AID-PTR584>3.0.CO;2-D","article-title":"A phase I trial of andrographolide in HIV positive patients and normal volunteers","volume":"14","author":"Calabrese","year":"2000","journal-title":"Phytother. Res."},{"key":"10.1016\/j.jep.2016.12.029_bib11","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/650812","article-title":"SOCS3 expression correlates with severity of inflammation, expression of proinflammatory cytokines, and activation of STAT3 and p38 MAPK in LPS-induced inflammation in vivo","author":"Chaves de Souza","year":"2013","journal-title":"Mediat. Inflamm."},{"issue":"9","key":"10.1016\/j.jep.2016.12.029_bib12","doi-asserted-by":"crossref","first-page":"5652","DOI":"10.4049\/jimmunol.176.9.5652","article-title":"STAT3 and NF-\u03baB signal pathway is required for IL-23-mediated IL-17 production in spontaneous arthritis animal model IL-1 receptor antagonist-deficient mice","volume":"176","author":"Cho","year":"2006","journal-title":"J. Immunol."},{"issue":"2","key":"10.1016\/j.jep.2016.12.029_bib13","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/BF02829954","article-title":"Techniques for quantifying effects of dietary antioxidants on transcription factor translocation and nitric oxide production in cultured cells","volume":"1","author":"Ewins","year":"2006","journal-title":"Genes Nutr."},{"issue":"21\u201322","key":"10.1016\/j.jep.2016.12.029_bib14","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.lfs.2009.03.001","article-title":"Signaling pathways associated with the expression of inflammatory mediators activated during the course of two models of experimental periodontitis","volume":"84","author":"Garcia de Aquino","year":"2009","journal-title":"Life Sci."},{"issue":"51","key":"10.1016\/j.jep.2016.12.029_bib15","doi-asserted-by":"crossref","first-page":"6887","DOI":"10.1038\/sj.onc.1209982","article-title":"Inhibitors of NF-\u03baB signaling: 785 and counting","volume":"25","author":"Gilmore","year":"2006","journal-title":"Oncogene"},{"key":"10.1016\/j.jep.2016.12.029_bib16","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/986264","article-title":"LPS from P. gingivalis and hypoxia increases oxidative stress in periodontal ligament fibroblasts and contributes to periodontitis","volume":"2014","author":"G\u00f6lz","year":"2014","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.jep.2016.12.029_bib17","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/438085","article-title":"Hypoxia and P. gingivalis synergistically induce HIF-1 and NF-\u03baB activation in PDL cells and periodontal diseases","volume":"2015","author":"G\u00f6lz","year":"2015","journal-title":"Mediat. Inflamm."},{"issue":"1","key":"10.1016\/j.jep.2016.12.029_bib18","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.cytogfr.2009.11.005","article-title":"Dangerous liaisons: STAT3 and NF-\u03baB collaboration and crosstalk in cancer","volume":"21","author":"Grivennikov","year":"2010","journal-title":"Cytokine Growth Factor Rev."},{"issue":"4","key":"10.1016\/j.jep.2016.12.029_bib19","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.intimp.2012.09.002","article-title":"Water-soluble andrographolide sulfonate exerts anti-sepsis action in mice through down-regulating p38 MAPK, STAT3 and NF-\u03baB pathways","volume":"14","author":"Guo","year":"2012","journal-title":"Int. Immunopharmacol."},{"issue":"1","key":"10.1016\/j.jep.2016.12.029_bib20","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.it.2013.09.001","article-title":"Immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host response","volume":"35","author":"Hajishengallis","year":"2014","journal-title":"Trends Immunol."},{"key":"10.1016\/j.jep.2016.12.029_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2016.07.018","article-title":"Andrographispaniculata extract attenuates pathological cardiac hypertrophy and apoptosis in high-fat diet fed mice","author":"Hsieh","year":"2016","journal-title":"J. Ethnopharmacol."},{"issue":"4","key":"10.1016\/j.jep.2016.12.029_bib22","doi-asserted-by":"crossref","first-page":"e0152770","DOI":"10.1371\/journal.pone.0152770","article-title":"Development of a bifunctional andrographolide-basedandrographolide-based chemical probe for pharmacological study","volume":"11","author":"Hsu","year":"2016","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.jep.2016.12.029_bib23","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1159\/000430196","article-title":"Andrographolide exerts pro-osteogenic effect by activation of wnt\/\u03b2-catenin signaling pathway in vitro","volume":"36","author":"Jiang","year":"2015","journal-title":"Cell. Physiol. Biochem."},{"issue":"6","key":"10.1016\/j.jep.2016.12.029_bib24","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/nm1054","article-title":"Selective inhibition of NF-\u03baB blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo","volume":"10","author":"Jimi","year":"2004","journal-title":"Nat. Med."},{"issue":"6","key":"10.1016\/j.jep.2016.12.029_bib25","doi-asserted-by":"crossref","first-page":"185","DOI":"10.5051\/jpis.2012.42.6.185","article-title":"Anti-inflammatory effect of (-)-epigallocatechin-3-gallate on Porphyromonas gingivalis lipopolysaccharide-stimulated fibroblasts and stem cells derived from human periodontal ligament","volume":"42","author":"Jung","year":"2012","journal-title":"J. Periodontal Implant Sci."},{"issue":"3","key":"10.1016\/j.jep.2016.12.029_bib26","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1086\/515165","article-title":"Host modulation as a therapeutic strategy in the treatment of periodontal disease","volume":"28","author":"Kornman","year":"1999","journal-title":"Clin. Infect. Dis."},{"issue":"3","key":"10.1016\/j.jep.2016.12.029_bib27","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.jep.2011.03.068","article-title":"Andrographolide acts as an anti-inflammatory agent in LPS-stimulated RAW264. 7 macrophages by inhibiting STAT3-mediated suppression of the NF-\u03baB pathway","volume":"135","author":"Lee","year":"2011","journal-title":"J. Ethnopharmacol."},{"issue":"3","key":"10.1016\/j.jep.2016.12.029_bib28","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.phymed.2011.11.012","article-title":"Suppression of matrix metalloproteinase-9 expression by andrographolide in human monocytic THP-1 cells via inhibition of NF-\u03baB activation","volume":"19","author":"Lee","year":"2012","journal-title":"Phytomedicine"},{"issue":"8","key":"10.1016\/j.jep.2016.12.029_bib29","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1007\/s11095-008-9571-x","article-title":"Discovery and development of toll-like receptor 4 (TLR4) antagonists: a new paradigm for treating sepsis and other diseases","volume":"25","author":"Leon","year":"2008","journal-title":"Pharm. Res."},{"issue":"9","key":"10.1016\/j.jep.2016.12.029_bib30","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1172\/JCI0215650","article-title":"What does Stat3 do?","volume":"109","author":"Levy","year":"2002","journal-title":"J. Clin. Investig."},{"issue":"3","key":"10.1016\/j.jep.2016.12.029_bib31","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1093\/carcin\/bgq278","article-title":"Guggulsterone (GS) inhibits smokeless tobacco and nicotine-induced NF-\u03baB and STAT3 pathways in head and neck cancer cells","volume":"32","author":"Macha","year":"2011","journal-title":"Carcinogenesis"},{"issue":"8","key":"10.1016\/j.jep.2016.12.029_bib32","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1111\/j.1462-5822.2007.00931.x","article-title":"Intrinsic apoptotic pathways of gingival epithelial cells modulated by Porphyromonas gingivalis","volume":"9","author":"Mao","year":"2007","journal-title":"Cell. Microbiol."},{"issue":"1","key":"10.1016\/j.jep.2016.12.029_bib33","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2298\/ABS1401261M","article-title":"Mesenchymal stem cells isolated from human periodontal ligament","volume":"66","author":"Mileti\u0107","year":"2014","journal-title":"Arch. Biol. Sci."},{"issue":"3","key":"10.1016\/j.jep.2016.12.029_bib34","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1021\/np200906s","article-title":"Natural products as sources of new drugs over the 30 years from 1981 to 2010","volume":"75","author":"Newman","year":"2012","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.jep.2016.12.029_bib35","article-title":"Carranza's clinical periodontology","author":"Newman","year":"2011","journal-title":"Elsevier Health Sci."},{"issue":"9","key":"10.1016\/j.jep.2016.12.029_bib36","doi-asserted-by":"crossref","first-page":"e107407","DOI":"10.1371\/journal.pone.0107407","article-title":"Endocannabinoid and inflammatory response in periodontal ligament cells","volume":"9","author":"\u00d6zdemir","year":"2014","journal-title":"PloS One"},{"issue":"1","key":"10.1016\/j.jep.2016.12.029_bib37","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.1600-0757.1997.tb00199.x","article-title":"Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions","volume":"14","author":"Page","year":"1997","journal-title":"Periodontology"},{"issue":"49","key":"10.1016\/j.jep.2016.12.029_bib38","doi-asserted-by":"crossref","DOI":"10.1038\/sj.onc.1203239","article-title":"Activators and target genes of Rel\/NF-\u03baB transcription factors","volume":"18","author":"Pahl","year":"1999","journal-title":"Oncogene"},{"issue":"7","key":"10.1016\/j.jep.2016.12.029_bib39","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.2337\/diabetes.51.7.2241","article-title":"Activation of nuclear factor-\u03baB induced by diabetes and high glucose regulates a proapoptotic program in retinal pericytes","volume":"51","author":"Romeo","year":"2002","journal-title":"Diabetes"},{"issue":"15","key":"10.1016\/j.jep.2016.12.029_bib40","doi-asserted-by":"crossref","first-page":"9595","DOI":"10.1074\/jbc.M710092200","article-title":"Oxysterol and diabetes activate STAT3 and control endothelial expression of profilin-1 via OSBP1","volume":"283","author":"Romeo","year":"2008","journal-title":"J. Biol. Chem."},{"issue":"27","key":"10.1016\/j.jep.2016.12.029_bib41","doi-asserted-by":"crossref","first-page":"4280","DOI":"10.3748\/wjg.14.4280","article-title":"Role of cytokines in inflammatory bowel disease","volume":"14","author":"Sanchez-Mu\u00f1oz","year":"2008","journal-title":"World J. Gastroenterol."},{"issue":"3","key":"10.1016\/j.jep.2016.12.029_bib42","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.phymed.2009.12.001","article-title":"A randomized double blind placebo controlled clinical evaluation of extract of Andrographis paniculata (KalmCold\u2122) in patients with uncomplicated upper respiratory tract infection","volume":"17","author":"Saxena","year":"2010","journal-title":"Phytomedicine"},{"issue":"2","key":"10.1016\/j.jep.2016.12.029_bib43","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/j.1600-0765.2009.01229.x","article-title":"Gingival and periodontal ligament fibroblasts differ in their inflammatory response to viable Porphyromonas gingivalis","volume":"45","author":"Scheres","year":"2010","journal-title":"J. Periodontal Res."},{"issue":"6","key":"10.1016\/j.jep.2016.12.029_bib44","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"issue":"5","key":"10.1016\/j.jep.2016.12.029_bib45","first-page":"96","article-title":"Characterization studies on medicinal plant of Andrographis paniculata (NEES)","volume":"3","author":"Shalini","year":"2015","journal-title":"J. Med. Plants"},{"issue":"2","key":"10.1016\/j.jep.2016.12.029_bib46","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/jcpe.12186","article-title":"A challenge with Porphyromonas gingivalis differentially affects the osteoclastogenesis potential of periodontal ligament fibroblasts from periodontitis patients and non\u2010periodontitis donors","volume":"41","author":"Sokos","year":"2014","journal-title":"J. Clin. Periodontol."},{"issue":"2","key":"10.1016\/j.jep.2016.12.029_bib47","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1590\/S1678-77572012000200002","article-title":"Modulation of host cell signaling pathways as a therapeutic approach in periodontal disease","volume":"20","author":"Souza","year":"2012","journal-title":"J. Appl. Oral Sci."},{"issue":"6","key":"10.1016\/j.jep.2016.12.029_bib48","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.4049\/jimmunol.173.6.4207","article-title":"Andrographolide attenuates inflammation by inhibition of NF-\u03baB activation through covalent modification of reduced cysteine 62 of p50","volume":"173","author":"Xia","year":"2004","journal-title":"J. Immunol."},{"issue":"11","key":"10.1016\/j.jep.2016.12.029_bib49","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1101\/gad.1553707","article-title":"Unphosphorylated STAT3 accumulates in response to IL-6 and activates transcription by binding to NF-\u03baB","volume":"21","author":"Yang","year":"2007","journal-title":"Genes Dev."},{"issue":"3","key":"10.1016\/j.jep.2016.12.029_bib50","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1111\/bph.12463","article-title":"Andrographolide suppresses RANKL\u2010induced osteoclastogenesis in vitro and prevents inflammatory bone loss in vivo","volume":"171","author":"Zhai","year":"2014","journal-title":"Br. J. Pharmacol."},{"issue":"2","key":"10.1016\/j.jep.2016.12.029_bib51","doi-asserted-by":"crossref","first-page":"e56407","DOI":"10.1371\/journal.pone.0056407","article-title":"Andrographolide protects against LPS-induced acute lung injury by inactivation of NF-\u03baB","volume":"8","author":"Zhu","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.jep.2016.12.029_bib52","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1042\/bj20020588","article-title":"Signal transducers and activators of transcription 3 (STAT3) inhibits transcription of the inducible nitric oxide synthase gene by interacting with nuclear factor \u03baB","volume":"367","author":"Zhiyuan","year":"2002","journal-title":"Biochem. J."}],"container-title":["Journal of Ethnopharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874116323960?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874116323960?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T17:52:00Z","timestamp":1536169920000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378874116323960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":52,"alternative-id":["S0378874116323960"],"URL":"https:\/\/doi.org\/10.1016\/j.jep.2016.12.029","relation":{},"ISSN":["0378-8741"],"issn-type":[{"value":"0378-8741","type":"print"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An ex vivo evaluation of the efficacy of andrographolide in modulating differential expression of transcription factors and target genes in periodontal cells and its potential role in treating periodontal diseases","name":"articletitle","label":"Article Title"},{"value":"Journal of Ethnopharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jep.2016.12.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Ireland Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}