{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:52:41Z","timestamp":1768697561532,"version":"3.49.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["85776"],"award-info":[{"award-number":["85776"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ethnopharmacology"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.jep.2018.07.022","type":"journal-article","created":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T14:12:45Z","timestamp":1532355165000},"page":"148-155","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["1H NMR-based metabolomics of antimalarial plant species traditionally used by Vha-Venda people in Limpopo Province, South Africa and isolation of antiplasmodial compounds"],"prefix":"10.1016","volume":"228","author":[{"given":"M. Johanna","family":"Bapela","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0941-2506","authenticated-orcid":false,"given":"Heino","family":"Heyman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francois","family":"Senejoux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.J. Marion","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jep.2018.07.022_bib1","first-page":"157","article-title":"Flavonoidal content of Vangueria infausta extract grown in Egypt: investigation of its antioxidant activity","volume":"2","author":"Abeer","year":"2011","journal-title":"Int. Res. J. Pharm."},{"key":"10.1016\/j.jep.2018.07.022_bib2","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1021\/np800333r","article-title":"Bioactive diterpenes and other constituents of Croton steenkampianus","volume":"71","author":"Adelekan","year":"2008","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.jep.2018.07.022_bib3","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0022-1759(94)90396-4","article-title":"A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay","volume":"170","author":"Ahmed","year":"1994","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jep.2018.07.022_bib4","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1021\/jo00872a046","article-title":"Carbon-13 nuclear magnetic resonance analysis of vobasine-like indole alkaloids","volume":"41","author":"Ahond","year":"1976","journal-title":"J. Org. Chem."},{"key":"10.1016\/j.jep.2018.07.022_bib5","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0378-8741(84)90086-2","article-title":"Pharmacopoeia of traditional medicine in Venda","volume":"12","author":"Arnold","year":"1984","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2018.07.022_bib6","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.jpba.2003.12.024","article-title":"Prediction of anti-plasmodial activity of Artemisia annua extracts: application of 1H NMR spectroscopy and chemometrics","volume":"35","author":"Bailey","year":"2004","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jep.2018.07.022_bib7","first-page":"70","article-title":"African healthcare: emphasis on Mozambique","volume":"39","author":"Bandeira","year":"2001","journal-title":"Pharm. Biol."},{"key":"10.1016\/j.jep.2018.07.022_bib8","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.jep.2014.09.017","article-title":"In vitro antiplasmodial screening of ethnopharmacologically selected South African plant species used for the treatment of malaria","volume":"156","author":"Bapela","year":"2014","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2018.07.022_bib9","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.jpba.2013.03.007","article-title":"Isolation and characterization of bioactive compounds from plant resources: the role of analysis in the ethnopharmacological approach","volume":"87","author":"Brusotti","year":"2014","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jep.2018.07.022_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12936-016-1650-6","article-title":"Incidence and admission rates for severe malaria and their impact on mortality in Africa","volume":"16","author":"Camponovo","year":"2017","journal-title":"Malar. J."},{"key":"10.1016\/j.jep.2018.07.022_bib11","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s40249-016-0151-8","article-title":"Malaria: global progress 2000\u20132015 and future challenges Infect","volume":"5","author":"Cibulskis","year":"2016","journal-title":"Dis. Poverty"},{"key":"10.1016\/j.jep.2018.07.022_bib12","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/j.phytochem.2004.01.019","article-title":"Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique","volume":"65","author":"Choi","year":"2004","journal-title":"Phytochemistry"},{"key":"10.1016\/j.jep.2018.07.022_bib13","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/AAC.45.5.1349-1354.2001","article-title":"Antileishmanial activity of an indole alkaloid from Peschiera australis","volume":"45","author":"Delorenzi","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jep.2018.07.022_bib14","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1128\/AAC.16.6.710","article-title":"Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique","volume":"16","author":"Desjardins","year":"1979","journal-title":"Antimicrob. Agents Chemother."},{"issue":"5","key":"10.1016\/j.jep.2018.07.022_bib15","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.pt.2017.01.004","article-title":"How to contain artemisinin- and multidrug-resistant falciparum malaria","volume":"33","author":"Dondorp","year":"2017","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.jep.2018.07.022_bib16","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/j.1365-3156.2012.02963.x","article-title":"Optimal dose finding for novel antimalarial combination therapy","volume":"17","author":"Duparc","year":"2012","journal-title":"Trop. Med. Int. Health"},{"key":"10.1016\/j.jep.2018.07.022_bib17","series-title":"Multi- and Megavariate Data Analysis, Part 1: Basic Principles and Applications","author":"Eriksson","year":"2006"},{"key":"10.1016\/j.jep.2018.07.022_bib18","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.phytochem.2011.09.012","article-title":"Indole alkaloids from Muntafara sessilifolia with antiplasmodial and cytotoxic activities","volume":"73","author":"Girardot","year":"2012","journal-title":"Phytochemistry"},{"key":"10.1016\/j.jep.2018.07.022_bib19","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1021\/np5002362","article-title":"An iridoid glucoside and the related aglycones from Cornus florida","volume":"77","author":"He","year":"2014","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.jep.2018.07.022_bib20","first-page":"4","article-title":"Antimalarial compounds in phase II clinical development","volume":"24","author":"Held","year":"2014","journal-title":"Expert Opin. Investig. Drugs"},{"key":"10.1016\/j.jep.2018.07.022_bib21","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1016\/j.trac.2011.04.019","article-title":"Data-processing strategies for metabolomics studies","volume":"30","author":"Hendriks","year":"2011","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.jep.2018.07.022_bib22","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.sajb.2012.04.001","article-title":"NMR-based metabolomics as a quality control tool for herbal products","volume":"82","author":"Heyman","year":"2012","journal-title":"S. Afr. J. Bot."},{"key":"10.1016\/j.jep.2018.07.022_bib23","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.fitote.2015.03.024","article-title":"Identification of anti-HIV active dicaffeoylquinic- and tricaffeoylquinic acids in Helichrysum populifolium by NMR-based metabolomic guided fractionation","volume":"103","author":"Heyman","year":"2015","journal-title":"Fitoterapia"},{"key":"10.1016\/j.jep.2018.07.022_bib24","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0001-706X(93)90083-N","article-title":"A comparison of the three methods of estimating EC50 in studies of drug resistance of malaria parasites","volume":"55","author":"Huber","year":"1993","journal-title":"Acta Trop."},{"key":"10.1016\/j.jep.2018.07.022_bib25","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1038\/nprot.2009.237","article-title":"NMR-based metabolomic analysis of plants","volume":"5","author":"Kim","year":"2010","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jep.2018.07.022_bib26","doi-asserted-by":"crossref","first-page":"92","DOI":"10.3389\/fphar.2013.00092","article-title":"Traditional medicines and globalization: current and future perspectives in ethnopharmacology","volume":"4","author":"Leonti","year":"2013","journal-title":"Front. Pharmacol."},{"issue":"3","key":"10.1016\/j.jep.2018.07.022_bib27","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1248\/cpb.55.464","article-title":"Antiplasmodial activity of some constituents of the root bark of Harungana madagascariensis Lam. (Hypericaceae)","volume":"55","author":"Lenta","year":"2007","journal-title":"Chem. Pharm. Bull."},{"key":"10.1016\/j.jep.2018.07.022_bib28","series-title":"The Ethnobotany of the Vhavenda (Unpublished M.Sc. Thesis)","author":"Mabogo","year":"1990"},{"key":"10.1016\/j.jep.2018.07.022_bib29","doi-asserted-by":"crossref","first-page":"52","DOI":"10.5923\/j.chemistry.20110102.11","article-title":"Antimycobacterial friedelane-terpenoid from the root bark of Terminalia avicennioides","volume":"1","author":"Mann","year":"2011","journal-title":"Am. J. Chem."},{"key":"10.1016\/j.jep.2018.07.022_bib30","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.1016\/j.bmcl.2009.05.104","article-title":"Induction of apoptosis in HuH-7 cancer cells by monoterpene and \u03b2-carboline indole alkaloids isolated from the leaves of Tabernaemontana elegans","volume":"19","author":"Mansoor","year":"2009","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.jep.2018.07.022_bib31","series-title":"Immunological Methods","first-page":"221","article-title":"Plasmodium falciparum malaria parasite cultures and their use in immunology","author":"Matile","year":"1990"},{"key":"10.1016\/j.jep.2018.07.022_bib32","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.phytochem.2014.06.012","article-title":"What the devil is in your phytomedicine? Exploring species substitution in Harpagophytum through chemometric modeling of 1H NMR and UHPLC-MS datasets","volume":"106","author":"Mncwangi","year":"2014","journal-title":"Phytochemistry"},{"key":"10.1016\/j.jep.2018.07.022_bib33","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1248\/cpb.56.374","article-title":"Endodesmiadol, a friedelane triterpenoid, and other antiplasmodial compounds from Endodesmia calophylloides","volume":"56","author":"Ngouamegne","year":"2008","journal-title":"Chem. Pharm. Bull."},{"issue":"8","key":"10.1016\/j.jep.2018.07.022_bib34","doi-asserted-by":"crossref","first-page":"15159","DOI":"10.1038\/ncomms15159","article-title":"A tetraoxane-based antimalarial drug candidate that overcomes PfK13-C580Y dependent artemisinin resistance","volume":"24","author":"O\u2019Neill","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jep.2018.07.022_bib35","first-page":"50","article-title":"Chloroquine sensitivity of isolates of Plasmodium falciparum adapted to in vitro culture","volume":"33","author":"Ponnudurai","year":"1981","journal-title":"Trop. Geogr. Med."},{"key":"10.1016\/j.jep.2018.07.022_bib36","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0378-8741(01)00245-8","article-title":"In vitro antiplasmodial activity and cytotoxicity of ethnobotanically selected South African plants","volume":"76","author":"Prozesky","year":"2001","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2018.07.022_bib37","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.phytol.2016.08.004","article-title":"Potential role of metabolomics in the improvement of research on traditional African medicine","volume":"17","author":"Quansaha","year":"2016","journal-title":"Phytochem. Lett."},{"key":"10.1016\/j.jep.2018.07.022_bib38","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/S0031-9422(02)00716-1","article-title":"The role of chemical fingerprinting: application to Ephedra","volume":"62","author":"Schaneberg","year":"2003","journal-title":"Phytochemistry"},{"key":"10.1016\/j.jep.2018.07.022_bib39","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/pca.1185","article-title":"Application of NMR in plant metabolomics: techniques, problems and prospects","volume":"21","author":"Schripsema","year":"2010","journal-title":"Phytochem. Anal."},{"key":"10.1016\/j.jep.2018.07.022_bib40","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.jep.2012.01.050","article-title":"The potential of metabolic fingerprinting as a tool for the modernisation of TCM preparations","volume":"140","author":"Sheridan","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2018.07.022_bib41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cbpa.2008.01.032","article-title":"Metabolomics for phytomedicine research and drug development","volume":"12","author":"Shyur","year":"2008","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.jep.2018.07.022_bib42","doi-asserted-by":"crossref","first-page":"13439","DOI":"10.3390\/molecules171113439","article-title":"New triterpenes from Maytenus robusta: structural elucidation based on NMR experimental data and theoretical calculations","volume":"17","author":"Sousa","year":"2012","journal-title":"Molecules"},{"key":"10.1016\/j.jep.2018.07.022_bib43","first-page":"1","article-title":"Monoterpenes and related compounds from the medicinal plants of Africa","author":"Tchimene","year":"2013"},{"key":"10.1016\/j.jep.2018.07.022_bib44","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0254-6299(15)30440-3","article-title":"Some medicinal properties of Cussonia and Schefflera species used in traditional medicine","volume":"68","author":"Tetyana","year":"2002","journal-title":"S. Afr. J. Bot."},{"key":"10.1016\/j.jep.2018.07.022_bib45","doi-asserted-by":"crossref","first-page":"e1000402","DOI":"10.1371\/journal.pmed.1000402","article-title":"A research agenda for malaria eradication: drugs","volume":"8","author":"The MalERA Drugs Consultative Group on Drugs","year":"2011","journal-title":"PLoS Med."},{"key":"10.1016\/j.jep.2018.07.022_bib46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0378-8741(84)90046-1","article-title":"Tabernaemontana L. (Apocynaceae): a review of its taxonomy, phytochemistry, ethnobotany and pharmacology","volume":"10","author":"Van Beek","year":"1984","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2018.07.022_bib47","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1055\/s-2007-969101","article-title":"Indole alkaloids from Tabernaemontana elegans","volume":"2","author":"Van der Heijden","year":"1986","journal-title":"Planta Med."},{"key":"10.1016\/j.jep.2018.07.022_bib48","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.sajb.2007.10.004","article-title":"Metabolomic quality control of claimed anti-malarial Artemisia afra herbal remedy and A. afra and A. annua plant extracts","volume":"74","author":"Van der Kooy","year":"2008","journal-title":"S. Afr. J. Bot."},{"key":"10.1016\/j.jep.2018.07.022_bib49","series-title":"The Medicinal and Poisonous Plants of Southern and Eastern Africa","author":"Watt","year":"1962"},{"issue":"9918","key":"10.1016\/j.jep.2018.07.022_bib50","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0140-6736(13)60024-0","article-title":"Malaria","volume":"383","author":"White","year":"2014","journal-title":"Lancet"},{"key":"10.1016\/j.jep.2018.07.022_bib51","unstructured":"World Health Organization (WHO) 2017. World Malaria Report, Geneva."},{"key":"10.1016\/j.jep.2018.07.022_bib52","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1248\/cpb.43.1462","article-title":"Chemical constituents of Chinese natural medicine, Morindae radix, the dried roots of Morinda officinalis How.: structures of morindolide and morofficinaloside","volume":"43","author":"Yoshikawa","year":"1995","journal-title":"Chem. Pharm. Bull."}],"container-title":["Journal of Ethnopharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874117339387?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874117339387?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T18:20:44Z","timestamp":1542046844000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378874117339387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":52,"alternative-id":["S0378874117339387"],"URL":"https:\/\/doi.org\/10.1016\/j.jep.2018.07.022","relation":{},"ISSN":["0378-8741"],"issn-type":[{"value":"0378-8741","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"1H NMR-based metabolomics of antimalarial plant species traditionally used by Vha-Venda people in Limpopo Province, South Africa and isolation of antiplasmodial compounds","name":"articletitle","label":"Article Title"},{"value":"Journal of Ethnopharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jep.2018.07.022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}