{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:30:11Z","timestamp":1792711811281,"version":"4.5.0"},"reference-count":57,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission, India","doi-asserted-by":"publisher","award":["F.3-20\/2012"],"award-info":[{"award-number":["F.3-20\/2012"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016282","name":"Maharshi Dayanand University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100016282","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ethnopharmacology"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.jep.2020.113541","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T18:04:08Z","timestamp":1604340248000},"page":"113541","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":76,"special_numbering":"C","title":["Identification and characterization of new potent inhibitors of dengue virus NS5 proteinase from Andrographis paniculata supercritical extracts on in animal cell culture and in silico approaches"],"prefix":"10.1016","volume":"267","author":[{"given":"Sulochana","family":"Kaushik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalit","family":"Dar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samander","family":"Kaushik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaya Parkash","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jep.2020.113541_bib1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.virusres.2011.06.008","article-title":"Infectious dengue-1 virus entry into mosquito C6\/36 cells","volume":"16","author":"Acosta","year":"2011","journal-title":"Virus Res."},{"key":"10.1016\/j.jep.2020.113541_bib2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0378-8741(97)00099-8","article-title":"Testicular toxicity assessment of Andrographis paniculata dried extract in rats","volume":"58","author":"Burgos","year":"1997","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2020.113541_bib3","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/1099-1573(200008)14:5<333::AID-PTR584>3.0.CO;2-D","article-title":"A phase I trial of andrographolide in HIV positive patients and normal volunteers","volume":"14","author":"Calabrese","year":"2000","journal-title":"Phytother Res."},{"key":"10.1016\/j.jep.2020.113541_bib4","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jep.2010.03.007","article-title":"Effect of an extract of Andrographis paniculata leaves on inflammatory and allergic mediators in vitro","volume":"129","author":"Chandrasekaran","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2020.113541_bib5","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1007\/s11814-010-0128-4","article-title":"Application of gas anti-solvent process to the recovery of andrographolide from Andrographis paniculata Nees","volume":"27","author":"Charoenchaitrakool","year":"2010","journal-title":"Kor. J. Chem. Eng."},{"key":"10.1016\/j.jep.2020.113541_bib6","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1016\/j.bmcl.2014.03.060","article-title":"Synthesis, structure-activity relationships and biological evaluation of dehydro-andrographolide and andrographolide derivatives as novel anti-hepatitis B virus agents","volume":"24","author":"Chen","year":"2014","journal-title":"Bioorg. Med. Chem. Lett"},{"key":"10.1016\/j.jep.2020.113541_bib7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0021-9673(02)01831-9","article-title":"Separation of andrographolide and neo-andrographolide from the leaves of Andrographis paniculata using high-speed counter-current chromatography","volume":"984","author":"Du","year":"2003","journal-title":"J. Chromatogra. A."},{"key":"10.1016\/j.jep.2020.113541_bib8","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.jep.2004.07.008","article-title":"Anti-malarial activity of some xanthones isolated from the roots of Andrographis paniculata","volume":"95","author":"Dua","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2020.113541_bib9","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nprot.2016.051","article-title":"Computational protein-ligand docking and virtual drug screening with the Auto Dock suite","volume":"11","author":"Forli","year":"2016","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jep.2020.113541_bib10","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1078\/094471102321616391","article-title":"A double blind, placebo-controlled study of Andrographis paniculata fixed combination Kan Jang in the treatment of acute upper respiratory tract infections including sinusitis","volume":"9","author":"Gabrielian","year":"2002","journal-title":"Phytomedicine"},{"key":"10.1016\/j.jep.2020.113541_bib11","first-page":"373","article-title":"Dengue in India","volume":"136","author":"Gupta","year":"2012","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.jep.2020.113541_bib12","first-page":"1","article-title":"Computer-aided study of selective flavonoids against chikungunya virus replication using molecular docking and DFT-based approach","volume":"14","author":"Hussain","year":"2020","journal-title":"Struct. Chem."},{"key":"10.1016\/j.jep.2020.113541_bib13","first-page":"1","article-title":"Optimization of extraction method and qualitative FT-NMR analysis of Andrographis paniculata leaves","volume":"4","author":"Isha","year":"2013","journal-title":"Int. J. Food Sci. Technol."},{"key":"10.1016\/j.jep.2020.113541_bib14","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.jscs.2010.03.001","article-title":"High-performance thin layer chromatography method for estimation of andrographolide in herbal extract and polyherbal formulations","volume":"14","author":"Jain","year":"2010","journal-title":"J. Saudi Chem. Soc."},{"key":"10.1016\/j.jep.2020.113541_bib15","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1248\/jhs.54.370","article-title":"Pharmacological aspects of Andrographis paniculata on health and its major diterpenoid constituent andrographolide","volume":"54","author":"Jarukamjorn","year":"2008","journal-title":"J. Health Sci."},{"key":"10.1016\/j.jep.2020.113541_bib16","first-page":"169","article-title":"Andrographis paniculata: a review of its traditional uses, phytochemistry and pharmacology","volume":"3","author":"Joselin","year":"2014","journal-title":"Med. Aromatic Plants"},{"key":"10.1016\/j.jep.2020.113541_bib17","doi-asserted-by":"crossref","first-page":"177","DOI":"10.4103\/phrev.phrev_2_18","article-title":"Antiviral and therapeutic uses of medicinal plants and their derivatives against dengue viruses","volume":"12","author":"Kaushik","year":"2018","journal-title":"Phcog. Rev."},{"key":"10.1016\/j.jep.2020.113541_bib18","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.jep.2004.03.004","article-title":"Anticancer and immunostimulatory compounds from Andrographis paniculata","volume":"92","author":"Kumar","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2020.113541_bib19","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/S1004-9541(08)60018-X","article-title":"Supercritical carbon dioxide extraction of andrographolide from Andrographispaniculata: effect of the solvent flow rate, pressure, and temperature","volume":"15","author":"Kumoro","year":"2007","journal-title":"Chin. J. Chem. Eng."},{"key":"10.1016\/j.jep.2020.113541_bib20","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1021\/acs.iecr.8b02243","article-title":"Extraction of andrographolide from Andrographis paniculata dried leaves using supercritical CO2 and ethanol mixture","volume":"58","author":"Kumoro","year":"2018","journal-title":"Ind. Eng. Chem. Res."},{"key":"10.1016\/j.jep.2020.113541_bib21","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.1021\/ci200227u","article-title":"LigPlot+: multiple ligand-protein interaction diagrams for drug discovery","volume":"51","author":"Laskowski","year":"2011","journal-title":"J. Chem. Inf. Model."},{"key":"10.1016\/j.jep.2020.113541_bib22","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1093\/infdis\/jis351","article-title":"Dengue research opportunities in the Americas","volume":"206","author":"Laughlin","year":"2012","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jep.2020.113541_bib23","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1186\/1472-6882-13-192","article-title":"Effects of cocktail off our local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2","volume":"13","author":"Lee","year":"2013","journal-title":"BMC Compl. Alternative Med."},{"key":"10.1016\/j.jep.2020.113541_bib24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1248\/bpb.31.2018","article-title":"Inhibition of the epstein\u2013barr virus lytic cycle by andrographolide","volume":"31","author":"Lin","year":"2008","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.jep.2020.113541_bib25","series-title":"International Conference on Biological Environmental and Food Engineering: Bali, Indonesia","first-page":"4","article-title":"Inhibitory activities of methanol extracts of Andrographis paniculata and Ocimum sanctum against dengue-1 virus","author":"Ling","year":"2014"},{"key":"10.1016\/j.jep.2020.113541_bib26","doi-asserted-by":"crossref","first-page":"161","DOI":"10.4103\/2229-3485.192035","article-title":"Trends in clinical trials of dengue vaccine","volume":"7","author":"Marimuthu","year":"2016","journal-title":"Perspect. Clin. Res."},{"key":"10.1016\/j.jep.2020.113541_bib27","doi-asserted-by":"crossref","first-page":"15D-2","DOI":"10.1002\/9780471729259.mc15d02s27","article-title":"Dengue virus: isolation, propagation, quantification, and storage","volume":"27","author":"Medina","year":"2012","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.jep.2020.113541_bib28","first-page":"1","article-title":"Chemoprofiling of Andrographis paniculata (Kalmegh) for its andrographolide content in Madhya Pradesh, India","volume":"1","author":"Mishra","year":"2010","journal-title":"Int. J. Pharm. Biol. Sci."},{"key":"10.1016\/j.jep.2020.113541_bib29","first-page":"1954","article-title":"Isolation and characterisation of andrographolide from Andrographis paniculata","volume":"9","author":"Mohana","year":"2016","journal-title":"WJPPS"},{"key":"10.1016\/j.jep.2020.113541_bib30","first-page":"10","article-title":"Anti-dengue viral compounds from Andrographis paniculata by in silico approach","volume":"1","author":"Nithya","year":"2014","journal-title":"World. J. Alternat Med."},{"key":"10.1016\/j.jep.2020.113541_bib31","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.5068","article-title":"Using bioinformatics tools for the discovery of dengue RNA-dependent RNA polymerase inhibitors","volume":"6","author":"Nncube","year":"2018","journal-title":"Peer. J."},{"key":"10.1016\/j.jep.2020.113541_bib32","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S2222-1808(14)60509-0","article-title":"Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: a review of its phytochemistry and pharmacology","volume":"4","author":"Okhuarobo","year":"2014","journal-title":"Asian Pac. J. Trop. Dis."},{"key":"10.1016\/j.jep.2020.113541_bib33","article-title":"Improved tools and strategies for the prevention and control of arboviral diseases: a research-to-policy forum","volume":"12","author":"Olliaro","year":"2018","journal-title":"PLoSNegl. Trop. Dis"},{"key":"10.1016\/j.jep.2020.113541_bib34","first-page":"165","article-title":"Variation in morphological characteristics and andrographolide content in Andrographis paniculata (Burm. f.) Nees of Central India","volume":"1","author":"Pandey","year":"2010","journal-title":"IJEE"},{"key":"10.1016\/j.jep.2020.113541_bib35","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.antiviral.2016.12.014","article-title":"Activity of andrographolide against dengue virus","volume":"139","author":"Panraksa","year":"2017","journal-title":"Antivir. Res."},{"key":"10.1016\/j.jep.2020.113541_bib36","doi-asserted-by":"crossref","first-page":"473","DOI":"10.4103\/0250-474X.34570","article-title":"HPTLC analysis of hepatoprotective diterpenoid andrographolide from Andrographis paniculata Nees (Kalmegh)","volume":"69","author":"Raina","year":"2007","journal-title":"Indian J. Pharmaceut. Sci."},{"key":"10.1016\/j.jep.2020.113541_bib37","first-page":"147","article-title":"Andrographolide, a potential cancer therapeutic agent isolated from Andrographis paniculata","volume":"3","author":"Rajagopal","year":"2003","journal-title":"JETO"},{"key":"10.1016\/j.jep.2020.113541_bib38","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1076\/1388-0209(200007)3831-SFT204","article-title":"A rapid method for isolation of andrographolide from Andrographis paniculata Nees (Kalmegh)","volume":"38","author":"Rajani","year":"2000","journal-title":"Pharm. Biol."},{"key":"10.1016\/j.jep.2020.113541_bib39","doi-asserted-by":"crossref","first-page":"87","DOI":"10.4103\/ayu.AYU_144_17","article-title":"Anti-dengue activity of Andrographis paniculata extracts and quantification of dengue viral inhibition by SYBR green reverse transcription polymerase chain reaction","volume":"39","author":"Ramalingam","year":"2018","journal-title":"Ayu"},{"key":"10.1016\/j.jep.2020.113541_bib40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.11113\/jt.v69.3175","article-title":"Efficacy of biological activity of Andrographis paniculata extracted by using supercritical carbon dioxide (SC-CO2) extraction","volume":"69","author":"Rithwan","year":"2014","journal-title":"J. Teknol."},{"key":"10.1016\/j.jep.2020.113541_bib41","first-page":"286","article-title":"Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay","volume":"31","author":"Rothan","year":"2014","journal-title":"Trop. Biomed."},{"key":"10.1016\/j.jep.2020.113541_bib42","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.virol.2009.03.025","article-title":"Cholesterol biosynthesis modulation regulates dengue viral replication","volume":"389","author":"Rothwell","year":"2009","journal-title":"Virology"},{"key":"10.1016\/j.jep.2020.113541_bib43","first-page":"1","article-title":"Extraction, isolation and characterization of bioactive compounds from plants' extracts","volume":"8","author":"Sasidharan","year":"2011","journal-title":"AJTCAM"},{"key":"10.1016\/j.jep.2020.113541_bib44","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1002\/ptr.6209","article-title":"Resveratrol derivatives from Commiphora africana (A. Rich.) Endl. display cytotoxicity and selectivity against several human cancer cell lines","volume":"33","author":"Segun","year":"2019","journal-title":"Phytother Res."},{"key":"10.1016\/j.jep.2020.113541_bib45","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1007\/s00253-018-9488-1","article-title":"Green synthesis of silver nanoparticles from medicinal plants and evaluation of their antiviral potential against chikungunya virus","volume":"103","author":"Sharma","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jep.2020.113541_bib46","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jpba.2012.03.034","article-title":"HPTLC methods to assay active ingredients in pharmaceutical formulations: a review of the method development and validation steps","volume":"66","author":"Shewiyo","year":"2012","journal-title":"J. Pharmaceut. Biomed. Anal."},{"key":"10.1016\/j.jep.2020.113541_bib47","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.jep.2007.10.006","article-title":"Ethnobotanical survey of folk plants for the treatment of snakebites in Southern part of Tamil Nadu, India","volume":"115","author":"Samy","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2020.113541_bib48","doi-asserted-by":"crossref","first-page":"190","DOI":"10.22146\/ijc.21163","article-title":"Quantification of andrographolide isolated from Andrographis paniculata nees obtained from traditional market in yoga karta using validated HPLC","volume":"16","author":"Syukri","year":"2016","journal-title":"Indones. J. Chem."},{"key":"10.1016\/j.jep.2020.113541_bib49","first-page":"119","article-title":"Isolation and characterisation of andrographolide from Andrographis paniculata (burm. F) wall. Ex nees and its total flavonoid effects from Kemaman, Malaysia","volume":"8","author":"Sani","year":"2017","journal-title":"Int. J. Pharm. Qual. Assur."},{"key":"10.1016\/j.jep.2020.113541_bib50","first-page":"2","article-title":"Screening of anti-dengue activity in methanolic extracts of medicinal plants","volume":"12","author":"Tang","year":"2012","journal-title":"BMC Compl. Alternative Med."},{"key":"10.1016\/j.jep.2020.113541_bib51","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1080\/10496475.2011.602471","article-title":"Biotechnology and pharmacological evaluation of medicinal plants: an overview","volume":"17","author":"Thatoi","year":"2011","journal-title":"J. Herbs, Spices, Med. Plants"},{"key":"10.1016\/j.jep.2020.113541_bib52","first-page":"1","article-title":"Computational screening of medicinal plant phytochemicals to discover potent pan-serotype inhibitors against dengue virus","volume":"9","author":"Ul Qamar","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jep.2020.113541_bib53","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1002\/ptr.1765","article-title":"Antiviral properties of ent-labdene diterpenes of Andrographis paniculata Nees, inhibitors of herpes simplex virus type 1","volume":"19","author":"Wiart","year":"2005","journal-title":"Phytother Res."},{"key":"10.1016\/j.jep.2020.113541_bib54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep14179","article-title":"Activity of andrographolide against chikungunya virus infection","volume":"5","author":"Wintachai","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jep.2020.113541_bib55","series-title":"Dengue Control","author":"World Health Organization (Who)","year":"2018"},{"key":"10.1016\/j.jep.2020.113541_bib56","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1007\/s11655-014-1860-0","article-title":"Andrographolide as an anti-H1N1 drug and the mechanism related toretinoic acid-inducible gene-I-like receptors signaling pathway","volume":"20","author":"Yu","year":"2014","journal-title":"Chin. J. Integr. Med."},{"key":"10.1016\/j.jep.2020.113541_bib57","first-page":"976","article-title":"Development and validation of HPTLC method for estimation of hepatoprotective diterpenoid andrographolide in polyherbal formulations","volume":"5","author":"Zade","year":"2013","journal-title":"Int. J. Pharm. Pharmaceut. Sci."}],"container-title":["Journal of Ethnopharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874120334292?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874120334292?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:04:50Z","timestamp":1759943090000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378874120334292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":57,"alternative-id":["S0378874120334292"],"URL":"https:\/\/doi.org\/10.1016\/j.jep.2020.113541","relation":{},"ISSN":["0378-8741"],"issn-type":[{"value":"0378-8741","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification and characterization of new potent inhibitors of dengue virus NS5 proteinase from Andrographis paniculata supercritical extracts on in animal cell culture and in silico approaches","name":"articletitle","label":"Article Title"},{"value":"Journal of Ethnopharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jep.2020.113541","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"113541"}}