{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T06:05:44Z","timestamp":1782626744996,"version":"3.54.5"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"am","delay-in-days":264,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20231147"],"award-info":[{"award-number":["BK20231147"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ethnopharmacology"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.jep.2024.118690","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T21:45:55Z","timestamp":1723499155000},"page":"118690","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Bu-Sui-Dan Enhances Osteoblast Differentiation by Upregulating VGLL4 to Counteract TEAD4-Mediated RUNX2 Transcription Suppression in Ovariectomized Rats"],"prefix":"10.1016","volume":"335","author":[{"given":"Chao","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yitao","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jep.2024.118690_bib1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.cger.2021.05.012","article-title":"Bone health","volume":"37","author":"Akid","year":"2021","journal-title":"Clin. Geriatr. Med."},{"key":"10.1016\/j.jep.2024.118690_bib2","first-page":"56","article-title":"Discussion on the treatment of postmenopausal osteoporosis from thekidney based on the theory of \"bone marrow empty","volume":"13","author":"An-wei","year":"2018","journal-title":"China Journal of Pharmaceutical Economics"},{"key":"10.1016\/j.jep.2024.118690_bib3","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1101\/gad.350127.122","article-title":"YAP\u2013VGLL4 antagonism defines the major physiological function of the Hippo signaling effector YAP","volume":"36","author":"Cai","year":"2022","journal-title":"Genes Dev."},{"key":"10.1016\/j.jep.2024.118690_bib4","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.210357","article-title":"Snail regulates Hippo signalling-mediated cell proliferation and tissue growth in Drosophila","volume":"12","author":"Ding","year":"2022","journal-title":"Open Biol."},{"key":"10.1016\/j.jep.2024.118690_bib5","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1016\/S2213-8587(17)30184-5","article-title":"Use of bone turnover markers in postmenopausal osteoporosis","volume":"5","author":"Eastell","year":"2017","journal-title":"LANCET DIABETES ENDO"},{"key":"10.1016\/j.jep.2024.118690_bib6","doi-asserted-by":"crossref","DOI":"10.1186\/s12967-022-03341-7","article-title":"Melatonin increases bone mass in normal, perimenopausal, and postmenopausal osteoporotic rats via the promotion of osteogenesis","volume":"20","author":"Guan","year":"2022","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.jep.2024.118690_bib7","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2021.772944","article-title":"Eucommia, cuscuta, and drynaria extracts ameliorate glucocorticoid-induced osteoporosis by inhibiting osteoclastogenesis through PI3K\/akt pathway","volume":"12","author":"Han","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.jep.2024.118690_bib8","doi-asserted-by":"crossref","first-page":"8950","DOI":"10.1080\/21655979.2022.2056825","article-title":"Epigallocatechin gallate attenuates tumor necrosis factor (TNF)-\u03b1-induced inhibition of osteoblastic differentiation by up-regulating lncRNA TUG1 in osteoporosis","volume":"13","author":"Han","year":"2022","journal-title":"Bioengineered"},{"key":"10.1016\/j.jep.2024.118690_bib9","doi-asserted-by":"crossref","DOI":"10.1186\/s13075-021-02619-3","article-title":"Isopsoralen ameliorates rheumatoid arthritis by targeting MIF","volume":"23","author":"Han","year":"2021","journal-title":"Arthritis Res. Ther."},{"key":"10.1016\/j.jep.2024.118690_bib10","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.166888","article-title":"Glutathione limits RUNX2 oxidation and degradation to regulate bone formation","volume":"8","author":"Hu","year":"2023","journal-title":"JCI Insight"},{"key":"10.1016\/j.jep.2024.118690_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/plants10050945","article-title":"Anti-osteoporotic effects of commiphora Myrrha and its poly-saccharide via osteoclastogenesis inhibition","volume":"10","author":"Hwang","year":"2021","journal-title":"Plants"},{"key":"10.1016\/j.jep.2024.118690_bib12","doi-asserted-by":"crossref","DOI":"10.3390\/nu15204372","article-title":"Sword bean (Canavalia gladiata) pods induce differentiation in mc3t3-E1 osteoblast cells by activating the BMP2\/SMAD\/RUNX2 pathway","volume":"15","author":"Hwang","year":"2023","journal-title":"Nutrients"},{"key":"10.1016\/j.jep.2024.118690_bib13","doi-asserted-by":"crossref","first-page":"6573","DOI":"10.7150\/thno.55664","article-title":"The role of microRNAs in the osteogenic and chondrogenic differentiation of mesenchymal stem cells and bone pathologies","volume":"11","author":"Iaquinta","year":"2021","journal-title":"Theranostics"},{"key":"10.1016\/j.jep.2024.118690_bib14","first-page":"168","article-title":"Molecular mechanism of runx2-dependent bone development","volume":"43","author":"Komori","year":"2020","journal-title":"Mol. Cell"},{"key":"10.1016\/j.jep.2024.118690_bib15","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.ijbiomac.2022.11.176","article-title":"Circ_CUX1\/miR-130b-5p\/p300 axis for parathyroid hormone-stimulation of Runx2 activity in rat osteoblasts: a combined bioinformatic and experimental approach","volume":"225","author":"Krishnan","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.jep.2024.118690_bib16","doi-asserted-by":"crossref","DOI":"10.1186\/s10020-023-00649-y","article-title":"Myoblast-derived exosomal Prrx2 attenuates osteoporosis via transcriptional regulation of lncRNA-MIR22HG to activate Hippo pathway","volume":"29","author":"Li","year":"2023","journal-title":"Mol. Med."},{"key":"10.1016\/j.jep.2024.118690_bib17","doi-asserted-by":"crossref","DOI":"10.1186\/s13287-019-1494-4","article-title":"YAP as a key regulator of adipo-osteogenic differentiation in human MSCs","volume":"10","author":"Lorthongpanich","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.jep.2024.118690_bib18","doi-asserted-by":"crossref","DOI":"10.1530\/JOE-23-0116","article-title":"Postmenopausal osteoporosis coexisting with sarcopenia: the role and mechanisms of estrogen","volume":"259","author":"Lu","year":"2023","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.jep.2024.118690_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2023.116426","article-title":"Psoralen prevents the inactivation of estradiol and treats osteoporosis via covalently targeting HSD17B2","volume":"311","author":"Lu","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jep.2024.118690_bib21","doi-asserted-by":"crossref","DOI":"10.3390\/cancers15194689","article-title":"Estrogen receptor signaling in breast cancer","volume":"15","author":"Miziak","year":"2023","journal-title":"Cancers"},{"key":"10.1016\/j.jep.2024.118690_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2022.154509","article-title":"Equol exerts a protective effect on postmenopausal osteoporosis by upregulating OPG\/RANKL pathway","volume":"108","author":"Ni","year":"2023","journal-title":"Phytomedicine"},{"key":"10.1016\/j.jep.2024.118690_bib23","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/path.6069","article-title":"Muramyl dipeptide alleviates estrogen deficiency\u2010induced osteoporosis through canonical Wnt signaling","volume":"260","author":"Park","year":"2023","journal-title":"J. Pathol."},{"key":"10.1016\/j.jep.2024.118690_bib24","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.911326","article-title":"Role of traditional Chinese medicine in bone regeneration and osteoporosis","volume":"10","author":"Peng","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.jep.2024.118690_bib25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.maturitas.2021.02.007","article-title":"Postmenopausal osteoporosis coexisting with other metabolic diseases: treatment considerations","volume":"147","author":"Polyzos","year":"2021","journal-title":"Maturitas"},{"key":"10.1016\/j.jep.2024.118690_bib26","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.1032866","article-title":"Berbamine inhibits RANKL- and M-CSF-mediated osteoclastogenesis and alleviates ovariectomy-induced bone loss","volume":"13","author":"Qi","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.jep.2024.118690_bib27","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/S0140-6736(21)02646-5","article-title":"Drug therapy for osteoporosis in older adults","volume":"399","author":"Reid","year":"2022","journal-title":"Lancet"},{"key":"10.1016\/j.jep.2024.118690_bib28","doi-asserted-by":"crossref","first-page":"468","DOI":"10.5694\/mja2.50393","article-title":"Controversies in medicine: the role of calcium and vitamin D supplements in adults","volume":"211","author":"Reid","year":"2019","journal-title":"Med. J. Aust."},{"key":"10.1016\/j.jep.2024.118690_bib29","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/s41575-021-00571-w","article-title":"Hippo signalling in the liver: role in development, regeneration and disease","volume":"19","author":"Russell","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.jep.2024.118690_bib30","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1089\/scd.2018.0037","article-title":"Validation of osteogenic properties of cytochalasin D by high-resolution RNA-sequencing in mesenchymal stem cells derived from bone marrow and adipose tissues","volume":"27","author":"Samsonraj","year":"2018","journal-title":"Stem Cell. Dev."},{"key":"10.1016\/j.jep.2024.118690_bib31","doi-asserted-by":"crossref","DOI":"10.3390\/nu13051609","article-title":"Botanicals in postmenopausal osteoporosis","volume":"13","author":"S\u0142upski","year":"2021","journal-title":"Nutrients"},{"key":"10.1016\/j.jep.2024.118690_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2023.120601","article-title":"Structural characterization and anti-osteoporosis effects of polysaccharide purified from Eucommia ulmoides Oliver cortex based on its modulation on bone metabolism","volume":"306","author":"Song","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.jep.2024.118690_bib33","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aba4147","article-title":"VGLL4 promotes osteoblast differentiation by antagonizing TEADs-inhibited Runx2 transcription","volume":"6","author":"Suo","year":"2020","journal-title":"Sci. Adv."},{"key":"10.1016\/j.jep.2024.118690_bib34","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.730686","article-title":"The polymorphism at PLCB4 promoter (rs6086746) changes the binding affinity of RUNX2 and affects osteoporosis susceptibility: an analysis of bioinformatics-based case-control study and functional validation","volume":"12","author":"Tsai","year":"2021","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.jep.2024.118690_bib35","doi-asserted-by":"crossref","DOI":"10.1186\/s13287-023-03273-z","article-title":"Human umbilical cord mesenchymal stem cells conditioned medium exerts anti-tumor effects on KGN cells in a cell density-dependent manner through activation of the Hippo pathway","volume":"14","author":"Wan","year":"2023","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.jep.2024.118690_bib36","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1038\/s41569-018-0063-3","article-title":"The Hippo pathway in the heart: pivotal roles in development, disease, and regeneration","volume":"15","author":"Wang","year":"2018","journal-title":"Nat. Rev. Cardiol."},{"key":"10.1016\/j.jep.2024.118690_bib37","article-title":"Network pharmacology of iridoid glycosides from Eucommia ulmoides Oliver against osteoporosis","volume":"12","author":"Wang","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jep.2024.118690_bib38","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s13238-017-0371-0","article-title":"The Hippo pathway in tissue homeostasis and regeneration","volume":"8","author":"Wang","year":"2017","journal-title":"Protein Cell"},{"key":"10.1016\/j.jep.2024.118690_bib39","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1016\/j.cell.2015.05.029","article-title":"Glucose uptake and Runx2 synergize to orchestrate osteoblast differentiation and bone formation","volume":"161","author":"Wei","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.jep.2024.118690_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2019.101313","article-title":"The NOTCH1-dependent HIF1\u03b1\/VGLL4\/IRF2BP2 oxygen sensing pathway triggers erythropoiesis terminal differentiation","volume":"28","author":"Wang","year":"2020","journal-title":"Redox Biol."},{"key":"10.1016\/j.jep.2024.118690_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.canlet.2024.216764","article-title":"K48-linked deubiquitination of VGLL4 by USP15 enhances the efficacy of tumor immunotherapy in triple-negative breast cancer","volume":"588","author":"Wang","year":"2024","journal-title":"Cancer Lett."},{"issue":"5","key":"10.1016\/j.jep.2024.118690_bib42","first-page":"73","article-title":"Clinical study on modified tianjing busui pill combined with caltrate D in treatment of primary osteoporosis","volume":"22","author":"Xu","year":"2020","journal-title":"Journal of Hubei University of Chinese Medicine"},{"key":"10.1016\/j.jep.2024.118690_bib43","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.freeradbiomed.2022.12.102","article-title":"17\u03b2-estradiol plays the anti-osteoporosis role via a novel ESR1-Keap1-Nrf2 axis-mediated stress response activation and Tmem119 upregulation","volume":"195","author":"Yang","year":"2023","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.jep.2024.118690_bib44","article-title":"Psoralen alleviates radiation-induced bone injury by rescuing skeletal stem cell stemness through AKT-mediated upregulation of GSK-3\u03b2 and NRF2","volume":"13","author":"Yin","year":"2022","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.jep.2024.118690_bib45","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1002\/jbmr.3757","article-title":"The prevalence of osteoporosis in China, a nationwide, multicenter DXA survey","volume":"34","author":"Zeng","year":"2019","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.jep.2024.118690_bib46","doi-asserted-by":"crossref","first-page":"5399","DOI":"10.1096\/fj.201801797R","article-title":"Psoralen accelerates bone fracture healing by activating both osteoclasts and osteoblasts","volume":"33","author":"Zhang","year":"2019","journal-title":"Faseb. J."},{"key":"10.1016\/j.jep.2024.118690_bib47","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/cr.2014.10","article-title":"VGLL4 functions as a new tumor suppressor in lung cancer by negatively regulating the YAP-TEAD transcriptional complex","volume":"24","author":"Zhang","year":"2014","journal-title":"Cell Res."},{"key":"10.1016\/j.jep.2024.118690_bib48","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1142\/S0192415X22500173","article-title":"Advances in anti-osteoporosis polysaccharides derived from medicinal herbs and other edible substances","volume":"50","author":"Zhao","year":"2022","journal-title":"AM J CHINESE MED"},{"key":"10.1016\/j.jep.2024.118690_bib49","doi-asserted-by":"crossref","DOI":"10.1096\/fj.202101706R","article-title":"Thrap3 promotes osteogenesis by inhibiting the degradation of Runx2","volume":"36","author":"Zhou","year":"2022","journal-title":"Faseb. J."},{"key":"10.1016\/j.jep.2024.118690_bib50","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.bbi.2023.02.012","article-title":"ACSL4 promotes microglia-mediated neuroinflammation by regulating lipid metabolism and VGLL4 expression","volume":"109","author":"Zhou","year":"2023","journal-title":"Brain Behav. Immun."}],"container-title":["Journal of Ethnopharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874124009899?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378874124009899?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T08:12:05Z","timestamp":1777968725000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378874124009899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":49,"alternative-id":["S0378874124009899"],"URL":"https:\/\/doi.org\/10.1016\/j.jep.2024.118690","relation":{},"ISSN":["0378-8741"],"issn-type":[{"value":"0378-8741","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Bu-Sui-Dan Enhances Osteoblast Differentiation by Upregulating VGLL4 to Counteract TEAD4-Mediated RUNX2 Transcription Suppression in Ovariectomized Rats","name":"articletitle","label":"Article Title"},{"value":"Journal of Ethnopharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jep.2024.118690","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"118690"}}