{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:22:32Z","timestamp":1760059352420,"version":"build-2065373602"},"reference-count":76,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Equine Veterinary Science"],"published-print":{"date-parts":[[2021,4]]},"DOI":"10.1016\/j.jevs.2021.103394","type":"journal-article","created":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T06:42:52Z","timestamp":1612507372000},"page":"103394","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Genetic Manipulation of the Equine Oocyte and Embryo"],"prefix":"10.1016","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1090-1745","authenticated-orcid":false,"given":"Erin A.","family":"Hisey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo J.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8137-8384","authenticated-orcid":false,"given":"Stuart","family":"Meyers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0001","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1002\/mrd.20219","article-title":"Identification of mouse blastocyst genes that are downregulated by double-stranded RNA-mediated knockdown of Oct-4 expression","volume":"70","author":"Shin","year":"2005","journal-title":"Mol Reprod Dev"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0002","doi-asserted-by":"crossref","first-page":"7753","DOI":"10.1038\/s41598-018-25964-x","article-title":"Embryonic POU5F1 is required for expanded bovine blastocyst formation","volume":"8","author":"Daigneault","year":"2018","journal-title":"Sci Rep"},{"issue":"7674","key":"10.1016\/j.jevs.2021.103394_bib0003","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nature24033","article-title":"Genome editing reveals a role for OCT4 in human embryogenesis","volume":"550","author":"Fogarty","year":"2017","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0004","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/s41467-017-02706-7","article-title":"Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses","volume":"9","author":"Yoon","year":"2018","journal-title":"Nat Commun"},{"issue":"7","key":"10.1016\/j.jevs.2021.103394_bib0005","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.2217\/nnm.14.198","article-title":"Mesoporous silica-coated upconversion nanocrystals for near infrared light-triggered control of gene expression in zebrafish","volume":"10","author":"Jayakumar","year":"2015","journal-title":"Nanomedicine (Lond)"},{"issue":"12","key":"10.1016\/j.jevs.2021.103394_bib0006","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.3390\/genes10121053","article-title":"Danio Rerio as model organism for adenoviral vector evaluation","volume":"10","author":"Gul\u00edas","year":"2019","journal-title":"Genes (Basel)"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0007","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1038\/s41467-020-16173-0","article-title":"Multiplex precise base editing in cynomolgus monkeys","volume":"11","author":"Zhang","year":"2020","journal-title":"Nat Commun"},{"issue":"5","key":"10.1016\/j.jevs.2021.103394_bib0008","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s13238-015-0153-5","article-title":"CRISPR\/Cas9-mediated gene editing in human tripronuclear zygotes","volume":"6","author":"Liang","year":"2015","journal-title":"Protein Cell"},{"issue":"8","key":"10.1016\/j.jevs.2021.103394_bib0009","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1089\/hum.1995.6.8-1019","article-title":"Adenovirus-mediated gene transfer to the respiratory tract of fetal sheep in utero","volume":"6","author":"Vincent","year":"1995","journal-title":"Hum Gene Ther"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0010","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1089\/hum.2010.007","article-title":"Recombinant adeno-associated virus-mediated in utero gene transfer gives therapeutic transgene expression in the sheep","volume":"22","author":"David","year":"2011","journal-title":"Hum Gene Ther"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0011","first-page":"25","article-title":"Diploid parthenogenetic mouse embryos produced by heat-shock and Cytochalasin B","volume":"35","author":"Balakier","year":"1976","journal-title":"J Embryol Exp Morphol"},{"key":"10.1016\/j.jevs.2021.103394_bib0012","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1093\/biolreprod\/52.monograph_series1.319","article-title":"Activation of Horse Oocytes","volume":"1","author":"Hinrichs","year":"1995","journal-title":"Biol Reprod Monograph Ser"},{"issue":"Suppl 1","key":"10.1016\/j.jevs.2021.103394_bib0013","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/j.1365-2184.2008.00485.x","article-title":"Parthenotes as a source of embryonic stem cells","volume":"41","author":"Brevini","year":"2008","journal-title":"Cell Prolif"},{"issue":"3","key":"10.1016\/j.jevs.2021.103394_bib0014","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1095\/biolreprod65.3.899","article-title":"Influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol, and fetal calf serum on in vitro maturation and parthenogenic development in equine oocytes","volume":"65","author":"Carneiro","year":"2001","journal-title":"Biol Reprod"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0015","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1095\/biolreprod.104.029066","article-title":"Influence of insulin-like growth factor-I on cytoplasmic maturation of horse oocytes in vitro and organization of the first cell cycle following nuclear transfer and parthenogenesis","volume":"71","author":"Li","year":"2004","journal-title":"Biol Reprod"},{"issue":"7","key":"10.1016\/j.jevs.2021.103394_bib0016","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.theriogenology.2015.12.015","article-title":"Effect of clinically-related factors on in\u00a0vitro blastocyst development after equine ICSI","volume":"85","author":"Choi","year":"2016","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0017","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0093-691X(93)90341-2","article-title":"Bovine parthenogenetic blastocysts following in vitro maturation and oocyte activation with ethanol","volume":"40","author":"Minamihashi","year":"1993","journal-title":"Theriogenology"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0018","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1095\/biolreprod60.4.821","article-title":"Development of nuclear transfer and parthenogenetic rabbit embryos activated with inositol 1,4,5-trisphosphate","volume":"60","author":"Mitalipov","year":"1999","journal-title":"Biol Reprod"},{"issue":"5","key":"10.1016\/j.jevs.2021.103394_bib0019","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.theriogenology.2012.12.005","article-title":"A viable foal obtained by equine somatic cell nuclear transfer using oocytes recovered from immature follicles of live mares","volume":"79","author":"Choi","year":"2013","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0020","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1530\/rep.0.1220177","article-title":"Activation of cumulus-free equine oocytes: effect of maturation medium, calcium ionophore concentration and duration of cycloheximide exposure","volume":"122","author":"Choi","year":"2001","journal-title":"Reproduction"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0021","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1530\/rep.0.1260489","article-title":"Intracellular calcium oscillations and activation in horse oocytes injected with stallion sperm extracts or spermatozoa","volume":"126","author":"Bedford","year":"2003","journal-title":"Reproduction"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0022","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1095\/biolreprod.103.018200","article-title":"Activation of equine nuclear transfer oocytes: methods and timing of treatment in relation to nuclear remodeling","volume":"70","author":"Choi","year":"2004","journal-title":"Biol Reprod"},{"issue":"2","key":"10.1016\/j.jevs.2021.103394_bib0023","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1095\/biolreprod67.2.561","article-title":"Production of nuclear transfer horse embryos by Piezo-driven injection of somatic cell nuclei and activation with stallion sperm cytosolic extract","volume":"67","author":"Choi","year":"2002","journal-title":"Biol Reprod"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0024","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1017\/S0967199403001102","article-title":"In vitro development of equine nuclear transfer embryos: effects of oocyte maturation media and amino acid composition during embryo culture","volume":"11","author":"Choi","year":"2003","journal-title":"Zygote"},{"issue":"2","key":"10.1016\/j.jevs.2021.103394_bib0025","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1530\/reprod\/119.2.253","article-title":"Effects of different activation treatments on fertilization of horse oocytes by intracytoplasmic sperm injection","volume":"119","author":"Li","year":"2000","journal-title":"J Reprod Fertil"},{"key":"10.1016\/j.jevs.2021.103394_bib0026","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.ab.2014.11.005","article-title":"Asymmetric histone 3 methylation pattern between paternal and maternal pronuclei in equine zygotes","volume":"471","author":"Heras","year":"2015","journal-title":"Anal Biochem"},{"issue":"3","key":"10.1016\/j.jevs.2021.103394_bib0027","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1439-0531.2006.00759.x","article-title":"Heterospecific nuclear-transferred embryos derived from equine fibroblast cells and enucleated bovine oocytes","volume":"42","author":"Zhou","year":"2007","journal-title":"Reprod Domest Anim"},{"key":"10.1016\/j.jevs.2021.103394_bib0028","series-title":"Proceedings of the 5th International Symposium on Equine Embryo Transfer","article-title":"Nuclear transfer embryos in the horse","author":"Reggio","year":"2001"},{"issue":"1-2","key":"10.1016\/j.jevs.2021.103394_bib0029","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.anireprosci.2006.10.011","article-title":"Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer","volume":"98","author":"Galli","year":"2007","journal-title":"Anim Reprod Sci"},{"issue":"2","key":"10.1016\/j.jevs.2021.103394_bib0030","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1089\/clo.2008.0077","article-title":"Effect of sperm extract injection volume, injection of PLCzeta cRNA, and tissue cell line on efficiency of equine nuclear transfer","volume":"11","author":"Choi","year":"2009","journal-title":"Cloning Stem Cells"},{"key":"10.1016\/j.jevs.2021.103394_bib0031","series-title":"Proceedings of the 5th international symposium on equine embryo transfer Havemeyer Foundation Mono Ser","article-title":"Comparison of bovine and equine oocytes as host cytoplasts for equine nuclear transfer","author":"Hinrichs","year":"2001"},{"issue":"5636","key":"10.1016\/j.jevs.2021.103394_bib0032","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1126\/science.1086743","article-title":"A mule cloned from fetal cells by nuclear transfer","volume":"301","author":"Woods","year":"2003","journal-title":"Science"},{"issue":"6949","key":"10.1016\/j.jevs.2021.103394_bib0033","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1038\/424635a","article-title":"Pregnancy: a cloned horse born to its dam twin","volume":"424","author":"Galli","year":"2003","journal-title":"Nature"},{"issue":"Suppl 2","key":"10.1016\/j.jevs.2021.103394_bib0034","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/rda.13259","article-title":"Assisted reproductive techniques in mares","volume":"53","author":"Hinrichs","year":"2018","journal-title":"Reprod Domest Anim"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod.103.020982","article-title":"Cloned cattle fetuses with the same nuclear genetics are more variable than contemporary half-siblings resulting from artificial insemination and exhibit fetal and placental growth deregulation even in the first trimester","volume":"70","author":"Lee","year":"2004","journal-title":"Biol Reprod"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0036","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1095\/biolreprod.106.051581","article-title":"Large offspring or large placenta syndrome? Morphometric analysis of late gestation bovine placentomes from somatic nuclear transfer pregnancies complicated by hydrallantois","volume":"75","author":"Constant","year":"2006","journal-title":"Biol Reprod"},{"issue":"9","key":"10.1016\/j.jevs.2021.103394_bib0037","doi-asserted-by":"crossref","first-page":"983","DOI":"10.2460\/javma.236.9.983","article-title":"Physical and clinicopathologic findings in foals derived by use of somatic cell nuclear transfer: 14 cases (2004-2008)","volume":"236","author":"Johnson","year":"2010","journal-title":"J Am Vet Med Assoc"},{"key":"10.1016\/j.jevs.2021.103394_bib0038","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/978-1-4939-2848-4_16","article-title":"Neonatal care and management of foals derived by somatic cell nuclear transfer","volume":"1330","author":"Johnson","year":"2015","journal-title":"Methods Mol Biol"},{"issue":"6","key":"10.1016\/j.jevs.2021.103394_bib0039","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/j.theriogenology.2016.05.017","article-title":"Placental abnormalities in equine pregnancies generated by SCNT from one donor horse","volume":"86","author":"Pozor","year":"2016","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0040","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.theriogenology.2006.09.011","article-title":"Comparative aspects of somatic cell nuclear transfer with conventional and zona-free method in cattle, horse, pig and sheep","volume":"67","author":"Lagutina","year":"2007","journal-title":"Theriogenology"},{"issue":"2","key":"10.1016\/j.jevs.2021.103394_bib0041","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1530\/REP-07-0069","article-title":"Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and\/or ionomycin","volume":"134","author":"Hinrichs","year":"2007","journal-title":"Reproduction"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0042","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1095\/biolreprod.112.098855","article-title":"Equine cloning: in vitro and in vivo development of aggregated embryos","volume":"87","author":"Gambini","year":"2012","journal-title":"Biol Reprod"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0043","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1016\/j.theriogenology.2016.03.040","article-title":"Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse","volume":"86","author":"Gambini","year":"2016","journal-title":"Theriogenology"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0044","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1530\/rep.0.1250535","article-title":"Effect of the number of passages of fetal and adult fibroblasts on nuclear remodelling and first embryonic division in reconstructed horse oocytes after nuclear transfer","volume":"125","author":"Li","year":"2003","journal-title":"Reproduction"},{"issue":"7","key":"10.1016\/j.jevs.2021.103394_bib0045","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1071\/RD04025","article-title":"Cloned horse pregnancies produced using adult cumulus cells","volume":"16","author":"Vanderwall","year":"2004","journal-title":"Reprod Fertil Dev"},{"issue":"10","key":"10.1016\/j.jevs.2021.103394_bib0046","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0164049","article-title":"In vitro and in vivo development of horse cloned embryos generated with iPSCs, mesenchymal stromal cells and fetal or adult fibroblasts as nuclear donors","volume":"11","author":"Olivera","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.jevs.2021.103394_bib0047","first-page":"13","article-title":"Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses","volume":"11","author":"Olivera","year":"2018","journal-title":"Stem Cells Cloning"},{"issue":"3","key":"10.1016\/j.jevs.2021.103394_bib0048","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.theriogenology.2014.04.021","article-title":"Production of a mitochondrial-DNA identical cloned foal using oocytes recovered from immature follicles of selected mares","volume":"82","author":"Choi","year":"2014","journal-title":"Theriogenology"},{"issue":"3","key":"10.1016\/j.jevs.2021.103394_bib0049","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.theriogenology.2009.03.009","article-title":"Risk of equine infectious anemia virus disease transmission through in vitro embryo production using somatic cell nuclear transfer","volume":"72","author":"Gregg","year":"2009","journal-title":"Theriogenology"},{"issue":"2","key":"10.1016\/j.jevs.2021.103394_bib0050","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.theriogenology.2011.08.019","article-title":"The risk of introduction of equine infectious anemia virus into USA via cloned horse embryos imported from Canada","volume":"77","author":"Asseged","year":"2012","journal-title":"Theriogenology"},{"issue":"6","key":"10.1016\/j.jevs.2021.103394_bib0051","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1095\/biolreprod60.6.1496","article-title":"Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species","volume":"60","author":"Dominko","year":"1999","journal-title":"Biol Reprod"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0052","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF02698054","article-title":"Interspecies somatic cell nuclear transfer and preliminary data for horse-cow\/mouse iSCNT","volume":"2","author":"Tecirlioglu","year":"2006","journal-title":"Stem Cell Rev"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0053","first-page":"432","article-title":"Interspecies cloning using fresh, store and dead equine and bovine somatic cells as donor nuclei and bovine cytoplasts","volume":"57","author":"Li","year":"2002","journal-title":"Theriogenology"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0054","doi-asserted-by":"crossref","first-page":"76","DOI":"10.2746\/042516407X235786","article-title":"Liposome-mediated uptake of exogenous DNA by equine spermatozoa and applications in sperm-mediated gene transfer","volume":"40","author":"Ball","year":"2008","journal-title":"Equine Vet J"},{"issue":"5","key":"10.1016\/j.jevs.2021.103394_bib0055","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1002\/bies.20211","article-title":"Sperm-mediated gene transfer: applications and implications","volume":"27","author":"Smith","year":"2005","journal-title":"Bioessays"},{"issue":"7","key":"10.1016\/j.jevs.2021.103394_bib0056","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1071\/RD07172","article-title":"A unique method to produce transgenic embryos in ovine, porcine, feline, bovine and equine species","volume":"20","author":"Pereyra-Bonnet","year":"2008","journal-title":"Reprod Fertil Dev"},{"issue":"1-2","key":"10.1016\/j.jevs.2021.103394_bib0057","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.anireprosci.2012.12.010","article-title":"Expression of fluorescent reporter protein in equine embryos produced through intracytoplasmic sperm injection mediated gene transfer (ICSI-MGT)","volume":"137","author":"Zaniboni","year":"2013","journal-title":"Anim Reprod Sci"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0058","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/mrd.20316","article-title":"Multi-transgenic pigs expressing three fluorescent proteins produced with high efficiency by sperm mediated gene transfer","volume":"72","author":"Webster","year":"2005","journal-title":"Mol Reprod Dev"},{"issue":"22","key":"10.1016\/j.jevs.2021.103394_bib0059","doi-asserted-by":"crossref","first-page":"14230","DOI":"10.1073\/pnas.222550299","article-title":"Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation","volume":"99","author":"Lavitrano","year":"2002","journal-title":"Proc Natl Acad Sci U S A."},{"issue":"5","key":"10.1016\/j.jevs.2021.103394_bib0060","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/0092-8674(89)90787-3","article-title":"Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice","volume":"57","author":"Lavitrano","year":"1989","journal-title":"Cell"},{"issue":"2","key":"10.1016\/j.jevs.2021.103394_bib0061","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1081\/ABIO-100108336","article-title":"Expression of porcine growth hormone gene in transgenic rabbits as reported by green fluorescent protein","volume":"12","author":"Wang","year":"2001","journal-title":"Anim Biotechnol"},{"issue":"6213","key":"10.1016\/j.jevs.2021.103394_bib0062","doi-asserted-by":"crossref","DOI":"10.1126\/science.1258096","article-title":"Genome editing. The new frontier of genome engineering with CRISPR-Cas9","volume":"346","author":"Doudna","year":"2014","journal-title":"Science"},{"issue":"6121","key":"10.1016\/j.jevs.2021.103394_bib0063","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","author":"Cong","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.jevs.2021.103394_bib0064","doi-asserted-by":"crossref","first-page":"e00471","DOI":"10.7554\/eLife.00471","article-title":"RNA-programmed genome editing in human cells","volume":"2","author":"Jinek","year":"2013","journal-title":"Elife"},{"issue":"6121","key":"10.1016\/j.jevs.2021.103394_bib0065","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.1232033","article-title":"RNA-guided human genome engineering via Cas9","volume":"339","author":"Mali","year":"2013","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0066","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/cr.2013.45","article-title":"Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos","volume":"23","author":"Chang","year":"2013","journal-title":"Cell Res"},{"issue":"12","key":"10.1016\/j.jevs.2021.103394_bib0067","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1002\/dvg.22719","article-title":"Biallelic genome modification in F(0) Xenopus tropicalis embryos using the CRISPR\/Cas system","volume":"51","author":"Blitz","year":"2013","journal-title":"Genesis"},{"issue":"3","key":"10.1016\/j.jevs.2021.103394_bib0068","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1095\/biolreprod.114.121723","article-title":"Use of the CRISPR\/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos","volume":"91","author":"Whitworth","year":"2014","journal-title":"Biol Reprod"},{"issue":"8","key":"10.1016\/j.jevs.2021.103394_bib0069","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0136690","article-title":"Efficient generation of myostatin knock-out sheep using CRISPR\/Cas9 technology and microinjection into zygotes","volume":"10","author":"Crispo","year":"2015","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.jevs.2021.103394_bib0070","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.cell.2014.01.027","article-title":"Generation of gene-modified cynomolgus monkey via Cas9\/RNA-mediated gene targeting in one-cell embryos","volume":"156","author":"Niu","year":"2014","journal-title":"Cell"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0071","doi-asserted-by":"crossref","first-page":"7411","DOI":"10.1038\/s41598-020-62723-3","article-title":"Efficient correction of a deleterious point mutation in primary horse fibroblasts with CRISPR-Cas9","volume":"10","author":"Pinzon-Arteaga","year":"2020","journal-title":"Sci Rep"},{"year":"2019","series-title":"CRISPR\/Cas9-mediated gene editing in HERDA equine","author":"Hawkes","key":"10.1016\/j.jevs.2021.103394_bib0072"},{"issue":"6","key":"10.1016\/j.jevs.2021.103394_bib0073","article-title":"Edition of prostaglandin E2 receptors EP2 and EP4 by CRISPR\/Cas9 technology in equine adipose mesenchymal stem cells","volume":"10","author":"Man\u00e7anares","year":"2020","journal-title":"Animals (Basel)"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0074","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1071\/RDv31n1Ab20","article-title":"Generation of myostatin knockout horse embryos using clustered regularly interspaced short palindromic repeats\/CRISPR-associated gene 9 and somatic cell nuclear transfer","volume":"31","author":"Vichera","year":"2019","journal-title":"Reprod Fertility Dev"},{"issue":"1","key":"10.1016\/j.jevs.2021.103394_bib0075","doi-asserted-by":"crossref","first-page":"15587","DOI":"10.1038\/s41598-020-72040-4","article-title":"Generation of myostatin edited horse embryos using CRISPR\/Cas9 technology and somatic cell nuclear transfer","volume":"10","author":"Moro","year":"2020","journal-title":"Sci Rep"},{"key":"10.1016\/j.jevs.2021.103394_bib0076","doi-asserted-by":"crossref","DOI":"10.1016\/j.jevs.2020.103025","article-title":"Livestock gene editing by one-step embryo manipulation","volume":"89","author":"Navarro-Serna","year":"2020","journal-title":"J Equine Vet Sci"}],"container-title":["Journal of Equine Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0737080621000241?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0737080621000241?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:50:33Z","timestamp":1760032233000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0737080621000241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":76,"alternative-id":["S0737080621000241"],"URL":"https:\/\/doi.org\/10.1016\/j.jevs.2021.103394","relation":{},"ISSN":["0737-0806"],"issn-type":[{"type":"print","value":"0737-0806"}],"subject":[],"published":{"date-parts":[[2021,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genetic Manipulation of the Equine Oocyte and Embryo","name":"articletitle","label":"Article Title"},{"value":"Journal of Equine Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jevs.2021.103394","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"103394"}}