{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:02:43Z","timestamp":1786719763425,"version":"build-2736575974"},"reference-count":74,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002753","name":"Universidad Nacional de Colombia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002753","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Equine Veterinary Science"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.jevs.2026.106048","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:57:34Z","timestamp":1780718254000},"page":"106048","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Pentoxifylline and EDTA can modulate the capacitation of frozen\/thawed donkey sperm"],"prefix":"10.1016","volume":"165","author":[{"given":"L.F.","family":"Mendoza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8427-4433","authenticated-orcid":false,"given":"G.","family":"Restrepo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jevs.2026.106048_bib0001","article-title":"Domesticated donkeys \u2013 Part II: types and breeds","volume":"2012","author":"Yilmaz","year":"2012","journal-title":"Can J Anim Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0002","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.theriogenology.2009.07.026","article-title":"Sexual behavior and ejaculate characteristics in P\u00eaga donkeys (Equus asinus) mounting estrous horse mares (Equus caballus)","volume":"73","author":"Canisso","year":"2010","journal-title":"Theriogenology"},{"key":"10.1016\/j.jevs.2026.106048_bib0003","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.3390\/genes13111945","article-title":"Evolution, and research development of donkeys","volume":"13","author":"Wang","year":"2022","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.jevs.2026.106048_bib0004","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1080\/03009734.2019.1697911","article-title":"In vitro fertilisation in domestic mammals: a brief overview","volume":"125","author":"Sjunnesson","year":"2020","journal-title":"Ups J Med Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0005","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jevs.2018.02.024","article-title":"Comparison of whole and centrifuged egg yolk added to Kenney\u2019s and lactose-EDTA extenders for Donkey Semen cryopreservation","volume":"65","author":"Ferrante","year":"2018","journal-title":"J Equine Vet Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0006","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/854837","article-title":"Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART","volume":"2012","author":"Di Santo","year":"2012","journal-title":"Adv Urol"},{"key":"10.1016\/j.jevs.2026.106048_bib0007","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.theriogenology.2021.11.013","article-title":"Targeted antioxidant delivery modulates mitochondrial functions, ameliorates oxidative stress and preserve sperm quality during cryopreservation","volume":"179","author":"Tiwari","year":"2022","journal-title":"Theriogenology"},{"key":"10.1016\/j.jevs.2026.106048_bib0008","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jevs.2017.12.005","article-title":"A review of reproductive biology and biotechnologies in donkeys","volume":"65","author":"Miragaya","year":"2018","journal-title":"J Equine Vet Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0009","first-page":"607","article-title":"Key aspects of donkey and mule reproduction","volume":"35","author":"Canisso","year":"2019","journal-title":"Vet Clin North America: Equine Pract"},{"key":"10.1016\/j.jevs.2026.106048_bib0010","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1002\/mrd.22663","article-title":"Chang\u2019s meaning of capacitation: a molecular perspective","volume":"83","author":"Gervasi","year":"2016","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.jevs.2026.106048_bib0011","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.mod.2018.04.004","article-title":"Regulation mechanisms and implications of sperm membrane hyperpolarization","volume":"154","author":"Ritagliati","year":"2018","journal-title":"Mech Dev"},{"key":"10.1016\/j.jevs.2026.106048_bib0012","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1038\/181851a0","article-title":"Capacitation of mammalian spermatozoa","volume":"181","author":"Austin","year":"1958","journal-title":"Nature"},{"key":"10.1016\/j.jevs.2026.106048_bib0013","doi-asserted-by":"crossref","DOI":"10.1002\/mrd.23772","article-title":"Sperm metabolism","volume":"91","author":"Abruzzese","year":"2024","journal-title":"Mol Reprod Dev"},{"key":"10.1016\/j.jevs.2026.106048_bib0014","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4103\/1008-682X.167716","article-title":"Major regulatory mechanisms involved in sperm motility","volume":"19","author":"Pereira","year":"2017","journal-title":"Asian J Androl"},{"key":"10.1016\/j.jevs.2026.106048_bib0015","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1046\/j.1365-2605.2002.00348.x","article-title":"Effect of pentoxifylline on motility and membrane integrity of cryopreserved human spermatozoa","volume":"25","author":"Stanic","year":"2002","journal-title":"Int J Androl"},{"key":"10.1016\/j.jevs.2026.106048_bib0016","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jevs.2019.01.003","article-title":"Post-thaw addition of caffeine and\/or pentoxifylline affect differently motility of horse and donkey-cryopreserved spermatozoa","volume":"75","author":"Rota","year":"2019","journal-title":"J Equine Vet Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0017","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.jevs.2012.10.004","article-title":"Effects of pentoxifylline, caffeine, and taurine on post-thaw motility and longevity of equine frozen semen","volume":"33","author":"Stephens","year":"2013","journal-title":"J Equine Vet Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0018","doi-asserted-by":"crossref","DOI":"10.1016\/j.jevs.2021.103393","article-title":"L-proline: an effective agent for frozen and post-thawed donkey semen storage","volume":"101","author":"Li","year":"2021","journal-title":"J Equine Vet Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0019","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.cryobiol.2006.06.004","article-title":"Improvement in the long-term stability of freeze-dried mouse spermatozoa by adding of a chelating agent","volume":"53","author":"Kaneko","year":"2006","journal-title":"Cryobiology"},{"key":"10.1016\/j.jevs.2026.106048_bib0020","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1111\/j.1740-0929.2010.00856.x","article-title":"The addition of calcium ion chelator, EGTA to thawing solution improves fertilizing ability in frozen-thawed boar sperm","volume":"82","author":"Okazaki","year":"2011","journal-title":"Anim Sci J"},{"key":"10.1016\/j.jevs.2026.106048_bib0021","first-page":"1","article-title":"Feeding the stallion","author":"Warren","year":"2009","journal-title":"Agri-Facts"},{"key":"10.1016\/j.jevs.2026.106048_bib0022","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0093-691X(02)00750-1","article-title":"Post-thaw spermatozoa plasma membrane integrity and motility of stallion semen frozen with different cryoprotectants","volume":"58","author":"Henry","year":"2002","journal-title":"Theriogenology"},{"key":"10.1016\/j.jevs.2026.106048_bib0023","first-page":"103","article-title":"Effect on post-thaw viability of equine sperm using stepwise addition of dimethyl formamide and varying cooling and freezing procedures","volume":"5","author":"Perez-Osorio","year":"2008","journal-title":"Anim Reprod"},{"key":"10.1016\/j.jevs.2026.106048_bib0024","doi-asserted-by":"crossref","first-page":"333","DOI":"10.21071\/az.v66i255.2508","article-title":"Efecto de la suplementaci\u00f3n del diluyente sobre la calidad del semen de asno a la descongelaci\u00f3n","volume":"66","author":"Montoya","year":"2017","journal-title":"Archivos de Zootecnia"},{"key":"10.1016\/j.jevs.2026.106048_bib0025","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1111\/rda.14309","article-title":"Donkey semen cryopreservation: alternatives with permeable, non-permeable cryoprotectants and seminal plasma","volume":"58","author":"Montoya P\u00e1ez","year":"2023","journal-title":"Reprod Domestic Anim"},{"key":"10.1016\/j.jevs.2026.106048_bib0026","first-page":"159","article-title":"Effect of EDTA as chelating agent in extender on the post thaw quality of Buffalo bull spermatozoa","volume":"40","author":"Hussain","year":"2019","journal-title":"Cryo Lett"},{"key":"10.1016\/j.jevs.2026.106048_bib0027","doi-asserted-by":"crossref","first-page":"629","DOI":"10.3906\/vet-1501-16","article-title":"Heparin-induced in vitro capacitation changes of swamp buffalo spermatozoa","volume":"39","author":"Talukdar","year":"2015","journal-title":"Turk J Vet Anim Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0028","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1071\/RD17044","article-title":"Association of the cysteine-rich secretory protein-3 (CRISP-3) and some of its polymorphisms with the quality of cryopreserved stallion semen","volume":"30","author":"Alexandra","year":"2018","journal-title":"Reprod Fertil Dev"},{"key":"10.1016\/j.jevs.2026.106048_bib0029","doi-asserted-by":"crossref","first-page":"2908","DOI":"10.21897\/rmvz.260","article-title":"Efecto del colesterol y la dimetilformamida sobre par\u00e1metros posdescongelaci\u00f3n en espermatozoides de caballos criollos colombianos","volume":"17","author":"AnaM","year":"2012","journal-title":"Rev MVZ C\u00f3rdoba"},{"key":"10.1016\/j.jevs.2026.106048_bib0030","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1530\/jrf.0.0950755","article-title":"Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using Pisum sativum agglutinin","volume":"95","author":"Mendoza","year":"1992","journal-title":"Reproduction"},{"key":"10.1016\/j.jevs.2026.106048_bib0031","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1016\/j.theriogenology.2009.11.023","article-title":"Seasonal functional relevance of sperm characteristics in equine spermatozoa","volume":"73","author":"Gamboa","year":"2010","journal-title":"Theriogenology"},{"key":"10.1016\/j.jevs.2026.106048_bib0032","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/j.theriogenology.2005.08.022","article-title":"Capacitation-like changes in equine spermatozoa following cryopreservation","volume":"65","author":"Thomas","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.jevs.2026.106048_bib0033","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1084\/jem.183.4.1533","article-title":"Mitochondrial control of nuclear apoptosis","volume":"183","author":"Zamzami","year":"1996","journal-title":"J Exp Med"},{"key":"10.1016\/j.jevs.2026.106048_bib0034","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/rda.13599","article-title":"Membrane stability and mitochondrial activity of bovine sperm frozen with low-density lipoproteins and trehalose","volume":"55","author":"Varela","year":"2020","journal-title":"Reprod Domestic Anim"},{"key":"10.1016\/j.jevs.2026.106048_bib0035","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jevs.2026.106048_bib0036","doi-asserted-by":"crossref","first-page":"233","DOI":"10.22319\/rmcp.v8i3.4499","article-title":"Suplementaci\u00f3n con plasma seminal y relaci\u00f3n de sus componentes con la calidad de semen congelado-descongelado de asnos (Equus asinus)","volume":"8","author":"Montoya P\u00e1ez","year":"2017","journal-title":"Rev Mex Cienc Pecu"},{"key":"10.1016\/j.jevs.2026.106048_bib0037","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1093\/humrep\/13.4.911","article-title":"Correlation between intracellular cAMP content, kinematic parameters and hyperactivation of human spermatozoa after incubation with pentoxifylline","volume":"13","author":"Calogero","year":"1998","journal-title":"Hum Reprod"},{"key":"10.1016\/j.jevs.2026.106048_bib0038","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1477-7827-2-75","article-title":"Role of tyrosine phosphorylation in sperm capacitation\/acrosome reaction","volume":"2","author":"Naz","year":"2004","journal-title":"Reprod Biol Endocrinol"},{"key":"10.1016\/j.jevs.2026.106048_bib0039","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1002\/j.1939-4640.2002.tb02334.x","article-title":"Inhibition of alkaline phosphatase activity of boar semen by pentoxifylline, caffeine, and theophylline","volume":"23","author":"Jan","year":"2002","journal-title":"J Androl"},{"key":"10.1016\/j.jevs.2026.106048_bib0040","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1095\/biolreprod26.4.745","article-title":"Regulation of protein phosphorylation and motility of sperm by cyclic adenosine monophosphate and calcium","volume":"26","author":"Tash","year":"1982","journal-title":"Biol Reprod"},{"key":"10.1016\/j.jevs.2026.106048_bib0041","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/0006-2952(94)90477-4","article-title":"Phosphodiesterase inhibitory profile of some related xanthine derivatives pharmacologically active on the peripheral microcirculation","volume":"47","author":"Meskini","year":"1994","journal-title":"Biochem Pharmacol"},{"key":"10.1016\/j.jevs.2026.106048_bib0042","first-page":"241","article-title":"Additives effect of alpha-tocopherol, Pentoxifylline and Taurine on post thaw semen in Poitou donkeys","volume":"19","author":"Kumar","year":"2018","journal-title":"Vet Practitioner"},{"key":"10.1016\/j.jevs.2026.106048_bib0043","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1016\/S0015-0282(03)00267-X","article-title":"Calcium requirements for human sperm function in vitro","volume":"79","author":"Mar\u00edn-Briggiler","year":"2003","journal-title":"Fertil Steril"},{"key":"10.1016\/j.jevs.2026.106048_bib0044","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1439-0272.2010.01150.x","article-title":"Effect of procaine, pentoxifylline and trolox on capacitation and hyperactivation of stallion spermatozoa","volume":"44","author":"Ortgies","year":"2012","journal-title":"Andrologia"},{"key":"10.1016\/j.jevs.2026.106048_bib0045","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1093\/humupd\/dmn029","article-title":"Control of hyperactivation in sperm","volume":"14","author":"Suarez","year":"2008","journal-title":"Hum Reprod Update"},{"key":"10.1016\/j.jevs.2026.106048_bib0046","doi-asserted-by":"crossref","DOI":"10.1590\/1984-3143-ar2024-0043","article-title":"Sperm hyperactivation in the uterus and oviduct: a double-edged sword for sperm and maternal innate immunity toward fertility","volume":"21","author":"Akthar","year":"2024","journal-title":"Anim Reprod"},{"key":"10.1016\/j.jevs.2026.106048_bib0047","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1365-2125.1985.tb02611.x","article-title":"Calcium antagonists stimulate sperm motility in ejaculated human semen","volume":"19","author":"Hong","year":"1985","journal-title":"Br J Clin Pharmacol"},{"key":"10.1016\/j.jevs.2026.106048_bib0048","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1111\/j.1740-0929.2010.00856.x","article-title":"The addition of calcium ion chelator, EGTA to thawing solution improves fertilizing ability in frozen-thawed boar sperm","volume":"82","author":"Okazaki","year":"2011","journal-title":"Anim Sci J"},{"key":"10.1016\/j.jevs.2026.106048_bib0049","unstructured":"Keshtgar S, Gharesi-Fard B, Kazerooni M. Combined effect of Trolox and EDTA on frozen-thawed sperm quality. vol. 41. 2016."},{"key":"10.1016\/j.jevs.2026.106048_bib0050","article-title":"The effects of EGTA on the quality of fresh and cryopreserved-thawed Human spermatozoa","volume":"45","author":"Ebrahimi","year":"2020","journal-title":"IJMS"},{"key":"10.1016\/j.jevs.2026.106048_bib0051","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.cbpa.2011.05.026","article-title":"Roles of osmolality, calcium \u2014 potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm","volume":"160","author":"Alavi","year":"2011","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"10.1016\/j.jevs.2026.106048_bib0052","first-page":"252","article-title":"Studies on in vitro capacitation of goat spermatozoa by heparin treatment]","volume":"20","author":"Zhou","year":"2004","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"10.1016\/j.jevs.2026.106048_bib0053","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1439-0531.2006.00794.x","article-title":"Capacitation of frozen thawed buffalo bull (Bubalus bubalis) spermatozoa with higher heparin concentrations","volume":"42","author":"Mehmood","year":"2007","journal-title":"Reprod Domestic Anim"},{"key":"10.1016\/j.jevs.2026.106048_bib0054","doi-asserted-by":"crossref","first-page":"631","DOI":"10.4067\/S0716-97602006000500006","article-title":"Effect of heparin on in vitro capacitation of boar sperm","volume":"39","author":"Dapino","year":"2006","journal-title":"Biol Res"},{"key":"10.1016\/j.jevs.2026.106048_bib0055","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/S0093-691X(01)00446-0","article-title":"The effect of heparin on motility parameters and protein phosphorylation during bovine sperm capacitation","volume":"55","author":"Chamberland","year":"2001","journal-title":"Theriogenology"},{"key":"10.1016\/j.jevs.2026.106048_bib0056","first-page":"199","article-title":"Effect of heparin on capacitation\/acrosome reaction of equine sperm","volume":"31","author":"Varner","year":"1993","journal-title":"Syst Biol Reprod Med"},{"key":"10.1016\/j.jevs.2026.106048_bib0057","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1093\/biolreprod\/52.monograph_series1.713","article-title":"Use of Heparin to accelerate capacitation of equine spermatozoa In vivo","volume":"52","author":"Fleet","year":"1995","journal-title":"Biol Reprod"},{"key":"10.1016\/j.jevs.2026.106048_bib0058","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1046\/j.1365-2605.2002.00348.x","article-title":"Effect of pentoxifylline on motility and membrane integrity of cryopreserved human spermatozoa","volume":"25","author":"Stanic","year":"2002","journal-title":"Int J Androl"},{"key":"10.1016\/j.jevs.2026.106048_bib0059","first-page":"61","article-title":"L-carnitine and pentoxifylline supplementation improves sperm viability and motility at low temperature","volume":"17","author":"Nateghian","year":"2022","journal-title":"Int J Fertil Steril"},{"key":"10.1016\/j.jevs.2026.106048_bib0060","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/j.jevs.2013.05.002","article-title":"Effects of pentoxifylline on equine epididymal sperm","volume":"33","author":"Guasti","year":"2013","journal-title":"J Equine Vet Sci"},{"key":"10.1016\/j.jevs.2026.106048_bib0061","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3390\/oxygen5020008","article-title":"Pentoxifylline enhances sperm quality, reduces oxidative stress in semen, and decreases sperm DNA damage in men with asthenozoospermia undergoing assisted reproductive technology","volume":"5","author":"Ammar","year":"2025","journal-title":"Oxygen"},{"key":"10.1016\/j.jevs.2026.106048_bib0062","first-page":"154","article-title":"Efecto de la quercetina, la L-ergotioneina y la pentoxifilina en el semen equino posdescongelado","volume":"38","author":"Restrepo Betancur","year":"2016","journal-title":"Rev Salud Anim"},{"key":"10.1016\/j.jevs.2026.106048_bib0063","doi-asserted-by":"crossref","DOI":"10.1002\/rmb2.12614","article-title":"Molecular mechanisms of mammalian sperm capacitation, and its regulation by sodium-dependent secondary active transporters","volume":"23","author":"Takei","year":"2024","journal-title":"Reprod Med Biol"},{"key":"10.1016\/j.jevs.2026.106048_bib0064","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1111\/j.1365-2605.2006.00741.x","article-title":"Controlled cooling during semen cryopreservation does not induce capacitation of spermatozoa from two portions of the boar ejaculate","volume":"30","author":"Saravia","year":"2007","journal-title":"Int J Androl"},{"key":"10.1016\/j.jevs.2026.106048_bib0065","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0015-0282(16)56052-X","article-title":"Sperm treatment with pentoxifylline improves the fertilizing ability in patients with acrosome reaction insufficiency","volume":"60","author":"Tesarik","year":"1993","journal-title":"Fertil Steril"},{"key":"10.1016\/j.jevs.2026.106048_bib0066","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/aja.2010.183","article-title":"The role of antioxidant therapy in the treatment of male infertility: an overview","volume":"13","author":"Lombardo","year":"2011","journal-title":"Asian J Androl"},{"key":"10.1016\/j.jevs.2026.106048_bib0067","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/S0093-691X(00)00251-X","article-title":"Effect of trehalose and edta on cryoprotective action of ram semen diluents","volume":"53","author":"Aisen","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.jevs.2026.106048_bib0068","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1111\/j.1365-2605.1978.tb00619.x","article-title":"Destabilization of Human sperm membranes by albumin, EDTA and Histidine","volume":"1","author":"Johnsen","year":"1978","journal-title":"Int J Androl"},{"key":"10.1016\/j.jevs.2026.106048_bib0069","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/andr.12259","article-title":"Kinetic activity, membrane mitochondrial potential, lipid peroxidation, intracellular pH and calcium of frozen\/thawed bovine spermatozoa treated with metabolic enhancers","volume":"5","author":"Boni","year":"2017","journal-title":"Andrology"},{"key":"10.1016\/j.jevs.2026.106048_bib0070","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1017\/S096719942000012X","article-title":"bioenergetic and oxidative evaluations of donkey sperm preserved at +4\u00b0C","volume":"28","author":"T","year":"2020","journal-title":"Zygote"},{"key":"10.1016\/j.jevs.2026.106048_bib0071","doi-asserted-by":"crossref","DOI":"10.3390\/ani12233271","article-title":"Cryopreservation of semen in domestic animals: a review of current challenges, applications, and prospective strategies","volume":"12","author":"Sharafi","year":"2022","journal-title":"Animals"},{"key":"10.1016\/j.jevs.2026.106048_bib0072","first-page":"1","article-title":"Molecular basis of human sperm capacitation","volume":"6","author":"Molina","year":"2018","journal-title":"Front Cell Dev Biol"},{"key":"10.1016\/j.jevs.2026.106048_bib0073","doi-asserted-by":"crossref","first-page":"990","DOI":"10.3390\/ani15070990","article-title":"Current and emerging advanced techniques for breeding donkeys and mules","volume":"15","author":"Gambini","year":"2025","journal-title":"Animals (Basel)"},{"key":"10.1016\/j.jevs.2026.106048_bib0074","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jevs.2017.11.008","article-title":"The current situation and trend of donkey industry in Europe","volume":"65","author":"Camillo","year":"2018","journal-title":"J Equine Vet Sci"}],"container-title":["Journal of Equine Veterinary Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0737080626002832?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0737080626002832?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T14:19:22Z","timestamp":1786717162000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0737080626002832"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":74,"alternative-id":["S0737080626002832"],"URL":"https:\/\/doi.org\/10.1016\/j.jevs.2026.106048","relation":{},"ISSN":["0737-0806"],"issn-type":[{"value":"0737-0806","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pentoxifylline and EDTA can modulate the capacitation of frozen\/thawed donkey sperm","name":"articletitle","label":"Article Title"},{"value":"Journal of Equine Veterinary Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jevs.2026.106048","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"106048"}}