{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T17:32:27Z","timestamp":1787851947230,"version":"build-2784847793"},"reference-count":32,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Heilongjiang Province Natural Science Foundation","doi-asserted-by":"publisher","award":["LH2023C110"],"award-info":[{"award-number":["LH2023C110"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Research and Development Program of China"},{"DOI":"10.13039\/501100019939","name":"General Administration of Customs of the People's Republic of China","doi-asserted-by":"publisher","award":["2024HK014"],"award-info":[{"award-number":["2024HK014"]}],"id":[{"id":"10.13039\/501100019939","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the key research and development project of Hebei Province High-quality Development of Agriculture","award":["21322912D"],"award-info":[{"award-number":["21322912D"]}]},{"DOI":"10.13039\/501100019939","name":"General Administration of Customs of the People's Republic of China","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100019939","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Protection"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.jfp.2025.100554","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T12:09:50Z","timestamp":1748952590000},"page":"100554","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of a Test for the Real-time Fluorescence RNA Targeted Isothermal Amplification to Quickly Identify Cronobacter Species in Powdered Infant Formula"],"prefix":"10.1016","volume":"88","author":[{"given":"Ning","family":"Ding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaona","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muyun","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenrui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riqin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jfp.2025.100554_b0005","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.1128\/AEM.02470-08","article-title":"Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula","volume":"75","author":"Almeida","year":"2009","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0015","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.4315\/0362-028X-70.9.2111","article-title":"Dry stress and survival time of Enterobacter sakazakii and other Enterobacteriaceae in dehydrated powdered infant formula","volume":"70","author":"Barron","year":"2007","journal-title":"Journal of Food Protection"},{"key":"10.1016\/j.jfp.2025.100554_b0020","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1046\/j.1365-2672.2003.02067.x","article-title":"Desiccation and heat tolerance of Enterobacter sakazakii","volume":"95","author":"Breeuwer","year":"2003","journal-title":"Journal of Applied Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0025","article-title":"Rapid detection and simultaneous genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in powdered infant formula using real-time PCR and high resolution melting (HRM) analysis","volume":"8","author":"Cai","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jfp.2025.100554_b0030","unstructured":"Chen, Y., Lampel, K., & Hammack, T. (2012). Bacteriological analytical manual of FDA, Chapter 29: Cronobacter."},{"key":"10.1016\/j.jfp.2025.100554_b0035","unstructured":"Codex Alimentarius Commission (2008). Code of hygienic practice for powdered formulae for infants and young children."},{"key":"10.1016\/j.jfp.2025.100554_b0040","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.4315\/0362-028X-68.9.1900","article-title":"Survival of Enterobacter sakazakii in a dehydrated powdered infant formula","volume":"68","author":"Edelson-Mammel","year":"2005","journal-title":"Journal of Food Protection"},{"key":"10.1016\/j.jfp.2025.100554_b0050","doi-asserted-by":"crossref","first-page":"5430","DOI":"10.1128\/AEM.01390-15","article-title":"Genotyping and source tracking of Cronobacter sakazakii and C. malonaticus isolates from powdered infant formula and an infant formula production factory in China","volume":"81","author":"Fei","year":"2015","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0055","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1007\/s10096-009-0779-4","article-title":"Epidemiology of invasive neonatal Cronobacter (Enterobacter sakazakii) infections","volume":"28","author":"Friedemann","year":"2009","journal-title":"European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology"},{"issue":"1","key":"10.1016\/j.jfp.2025.100554_b0060","doi-asserted-by":"crossref","first-page":"5","DOI":"10.21307\/pjm-2019-001","article-title":"Antibiotic susceptibility of Cronobacter spp. isolated from clinical samples","volume":"68","author":"Hol\u00fd","year":"2019","journal-title":"Polish Journal of Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0065","unstructured":"International Organization for Standardization (2006). ISO-IDF 22964|IDF\/ RM 210: 2006. Specifies a method for the detection of Enterobacter sakazakii in milk and milk products."},{"key":"10.1016\/j.jfp.2025.100554_b0070","unstructured":"International Organization for Standardization (2017). Milk and milk products-detection of Enterbacter Sakazakii technical specification ISO\/TS 22964(E) and IDF\/RM 210: 2006, 1st Edn. (Geneva)."},{"key":"10.1016\/j.jfp.2025.100554_b0075","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1099\/ijs.0.65577-0","volume":"58","author":"Iversen","year":"2008","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0085","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1186\/1471-2180-9-225","article-title":"Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing","volume":"9","author":"Jaradat","year":"2009","journal-title":"BMC Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0090","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/1471-2180-4-43","article-title":"16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identification","volume":"4","author":"Lehner","year":"2004","journal-title":"BMC Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0095","article-title":"Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia","volume":"12","author":"Li","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.jfp.2025.100554_b0100","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1007\/s11274-011-0899-8","article-title":"Development of a loop-mediated isothermal amplification assay for detection of Cronobacter spp. (Enterobacter sakazakii)","volume":"28","author":"Liu","year":"2012","journal-title":"World Journal of Microbiology & Biotechnology"},{"key":"10.1016\/j.jfp.2025.100554_b0105","article-title":"Insights into the occurrence of Enterobacteriaceae and the spread of Cronobacter sakazakii in the infant formula manufacturing environment","author":"Lu","year":"2024","journal-title":"Food Bioscience"},{"key":"10.1016\/j.jfp.2025.100554_b0110","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.foodchem.2019.05.009","article-title":"Interaction of water activity and temperature on the growth, gene expression and aflatoxin production by Aspergillus flavus on paddy and polished rice","volume":"293","author":"Lv","year":"2019","journal-title":"Food Chemistry"},{"key":"10.1016\/j.jfp.2025.100554_b0115","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1111\/risa.12316","article-title":"The per case and total annual costs of foodborne illness in the United States","volume":"35","author":"Minor","year":"2015","journal-title":"Risk Analysis: An Official Publication of the Society for Risk Analysis"},{"key":"10.1016\/j.jfp.2025.100554_b0120","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1128\/AEM.72.4.2539-2546.2006","article-title":"Cloning and sequencing of the ompA gene of Enterobacter sakazakii and development of an ompA-targeted PCR for rapid detection of Enterobacter sakazakii in infant formula","volume":"72","author":"Mohan Nair","year":"2006","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0125","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3390\/bios14020097","article-title":"Current trends in RNA virus detection via nucleic acid isothermal amplification-based platforms","volume":"14","author":"Ngoc","year":"2024","journal-title":"Biosensors"},{"key":"10.1016\/j.jfp.2025.100554_b0130","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1111\/j.1472-765X.2009.02562.x","article-title":"Prevalence of Cronobacter species (Enterobacter sakazakii) in follow-on infant formulae and infant drinks","volume":"48","author":"O\u2019Brien","year":"2009","journal-title":"Letters in Applied Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0135","article-title":"Immunoinformatics assisted design of a multi-epitope kit for detecting Cronobacter sakazakii in powdered infant","volume":"8","author":"Oladipo","year":"2024","journal-title":"Formula"},{"key":"10.1016\/j.jfp.2025.100554_b0140","doi-asserted-by":"crossref","first-page":"34721","DOI":"10.1038\/srep34721","article-title":"Detection of Cronobacter sakazakii in powdered infant formula using an immunoliposome-based immunomagnetic concentration and separation assay","volume":"6","author":"Shukla","year":"2016","journal-title":"Scientific Reports"},{"key":"10.1016\/j.jfp.2025.100554_b0145","unstructured":"SN\/T1632.3-2013. Determination of Enterobacter sakazakii (Cronob\u03b1cter spp.) from dehydrated powdered milk for export: Part 3:Real-time PCR method."},{"key":"10.1016\/j.jfp.2025.100554_b0150","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/s00284-014-0708-3","article-title":"Development of fluorescence-based liposome immunoassay for detection of Cronobacter muytjensii in pure culture","volume":"70","author":"Song","year":"2015","journal-title":"Current Microbiology"},{"issue":"5","key":"10.1016\/j.jfp.2025.100554_b0155","doi-asserted-by":"crossref","first-page":"857","DOI":"10.3201\/eid2605.190858","article-title":"Food safety and invasive Cronobacter infections during early infancy, 1961\u20132018","volume":"26","author":"Strysko","year":"2020","journal-title":"Emerging Infectious Diseases"},{"key":"10.1016\/j.jfp.2025.100554_b0160","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.ijfoodmicro.2009.04.023","article-title":"Development and evaluation of rpoB based PCR systems to differentiate the six proposed species within the genus Cronobacter","volume":"136","author":"Stoop","year":"2009","journal-title":"International Journal of Food Microbiology"},{"key":"10.1016\/j.jfp.2025.100554_b0170","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.biotechadv.2009.12.004","article-title":"An overview of foodborne pathogen detection: in the perspective of biosensors","volume":"28","author":"Velusamy","year":"2010","journal-title":"Biotechnology Advances"},{"key":"10.1016\/j.jfp.2025.100554_b0175","doi-asserted-by":"crossref","first-page":"6067","DOI":"10.3168\/jds.2014-8287","article-title":"Detection of Cronobacter species in powdered infant formula by probe-magnetic separation PCR","volume":"97","author":"Xu","year":"2014","journal-title":"Journal of Dairy Science"},{"key":"10.1016\/j.jfp.2025.100554_b0180","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.fm.2014.02.010","article-title":"Development of a rapid detection system for opportunistic pathogenic Cronobacter spp. in powdered milk products","volume":"42","author":"Zimmermann","year":"2014","journal-title":"Food Microbiology"}],"container-title":["Journal of Food Protection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0362028X25001061?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0362028X25001061?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:07:07Z","timestamp":1762301227000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0362028X25001061"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["S0362028X25001061"],"URL":"https:\/\/doi.org\/10.1016\/j.jfp.2025.100554","relation":{},"ISSN":["0362-028X"],"issn-type":[{"value":"0362-028X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a Test for the Real-time Fluorescence RNA Targeted Isothermal Amplification to Quickly Identify Cronobacter Species in Powdered Infant Formula","name":"articletitle","label":"Article Title"},{"value":"Journal of Food Protection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jfp.2025.100554","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc. on behalf of International Association for Food Protection.","name":"copyright","label":"Copyright"}],"article-number":"100554"}}