{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T02:18:55Z","timestamp":1783477135674,"version":"3.55.0"},"reference-count":20,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["18fk0108062"],"award-info":[{"award-number":["18fk0108062"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP16K11061"],"award-info":[{"award-number":["JP16K11061"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Global Antimicrobial Resistance"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1016\/j.jgar.2019.02.011","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T12:37:52Z","timestamp":1551271072000},"page":"46-49","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["A real-time PCR assay for detecting a penA mutation associated with ceftriaxone resistance in Neisseria gonorrhoeae"],"prefix":"10.1016","volume":"19","author":[{"given":"Ken","family":"Shimuta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene","family":"Igawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Deguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-ichi","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Ohnishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jgar.2019.02.011_bib0005","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1186\/s12879-015-1029-2","article-title":"Current and future antimicrobial treatment of gonorrhoea \u2013 the rapidly evolving Neisseria gonorrhoeae continues to challenge","volume":"15","author":"Unemo","year":"2015","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.jgar.2019.02.011_bib0010","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1128\/AAC.00093-13","article-title":"Identification of amino acids conferring high-level resistance to expanded-spectrum cephalosporins in the penA gene from Neisseria gonorrhoeae strain H041","volume":"57","author":"Tomberg","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0015","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/332173a0","article-title":"Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae","volume":"332","author":"Spratt","year":"1988","journal-title":"Nature"},{"key":"10.1016\/j.jgar.2019.02.011_bib0020","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1056\/NEJMc1408109","article-title":"A new multidrug-resistant strain of Neisseria gonorrhoeae in Australia","volume":"371","author":"Lahra","year":"2014","journal-title":"N Engl J Med"},{"key":"10.1016\/j.jgar.2019.02.011_bib0025","doi-asserted-by":"crossref","first-page":"142","DOI":"10.3201\/eid2201.150868","article-title":"New clinical strain of Neisseria gonorrhoeae with decreased susceptibility to ceftriaxone, Japan","volume":"22","author":"Deguchi","year":"2016","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.jgar.2019.02.011_bib0030","doi-asserted-by":"crossref","first-page":"4339","DOI":"10.1128\/AAC.00504-16","article-title":"New ceftriaxone- and multidrug-resistant Neisseria gonorrhoeae strain with a novel mosaic penA gene isolated in Japan","volume":"60","author":"Nakayama","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0035","doi-asserted-by":"crossref","first-page":"381","DOI":"10.3201\/eid2402.171756","article-title":"Ceftriaxone-resistant Neisseria gonorrhoeae, Canada, 2017","volume":"24","author":"Lefebvre","year":"2018","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.jgar.2019.02.011_bib0040","doi-asserted-by":"crossref","first-page":"17","DOI":"10.2807\/1560-7917.ES.2017.22.42.17-00659","article-title":"Multidrug-resistant Neisseria gonorrhoeae infection with ceftriaxone resistance and intermediate resistance to azithromycin, Denmark, 2017","volume":"22","author":"Terkelsen","year":"2017","journal-title":"Euro Surveill"},{"key":"10.1016\/j.jgar.2019.02.011_bib0045","doi-asserted-by":"crossref","first-page":"735","DOI":"10.3201\/eid2404.171873","article-title":"Cooperative recognition of internationally disseminated ceftriaxone-resistant Neisseria gonorrhoeae strain","volume":"24","author":"Lahra","year":"2018","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.jgar.2019.02.011_bib0050","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2018.23.21.1800264","article-title":"Multidrug-resistant Neisseria gonorrhoeae failing treatment with ceftriaxone and doxycycline in France, November 2017","volume":"23","author":"Poncin","year":"2018","journal-title":"Euro Surveill"},{"key":"10.1016\/j.jgar.2019.02.011_bib0055","article-title":"Genomic surveillance of Neisseria gonorrhoeae to investigate the distribution and evolution of antimicrobial resistance determinants and lineages","volume":"4","author":"Yahara","year":"2018","journal-title":"Microb Genom"},{"key":"10.1016\/j.jgar.2019.02.011_bib0060","doi-asserted-by":"crossref","first-page":"e02069","DOI":"10.1128\/AAC.02069-17","article-title":"Neisseria cinerea with high ceftriaxone MIC is an origin of ceftriaxone resistance-mediated penA sequences in Neisseria gonorrhoeae","volume":"62","author":"Igawa","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0065","doi-asserted-by":"crossref","first-page":"5225","DOI":"10.1128\/AAC.01295-13","article-title":"Antimicrobial resistance and molecular typing of Neisseria gonorrhoeae isolates in Kyoto and Osaka, Japan, 2010 to 2012: intensified surveillance after identification of the first strain (H041) with high-level ceftriaxone resistance","volume":"57","author":"Shimuta","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0070","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1099\/mic.0.056077-0","article-title":"A genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus Neisseria","volume":"158","author":"Bennett","year":"2012","journal-title":"Microbiology"},{"key":"10.1016\/j.jgar.2019.02.011_bib0075","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.1093\/jac\/dkw288","article-title":"The novel 2016 WHO Neisseria gonorrhoeae reference strains for global quality assurance of laboratory investigations: phenotypic, genetic and reference genome characterization","volume":"71","author":"Unemo","year":"2016","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0080","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1128\/JCM.00100-17","article-title":"Neisseria gonorrhoeae sequence typing for antimicrobial resistance, a novel antimicrobial resistance multilocus typing scheme for tracking global dissemination of N. gonorrhoeae strains","volume":"55","author":"Demczuk","year":"2017","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jgar.2019.02.011_bib0085","doi-asserted-by":"crossref","first-page":"3538","DOI":"10.1128\/AAC.00325-11","article-title":"Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: Detailed characterization of the first strain with high-level resistance to ceftriaxone","volume":"55","author":"Ohnishi","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0090","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1128\/AAC.05760-11","article-title":"High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure","volume":"56","author":"Unemo","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0095","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1093\/jac\/dks162","article-title":"Molecular characterization of two high-level ceftriaxone-resistant Neisseria gonorrhoeae isolates detected in Catalonia, Spain","volume":"67","author":"C\u00e1mara","year":"2012","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.jgar.2019.02.011_bib0100","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.3201\/eid2408.180295","article-title":"Direct detection of penA gene associated with ceftriaxone-resistant Neisseria gonorrhoeae FC428 strain by using PCR","volume":"24","author":"Whiley","year":"2018","journal-title":"Emerg Infect Dis"}],"container-title":["Journal of Global Antimicrobial Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213716519300517?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213716519300517?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:56:30Z","timestamp":1761990990000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213716519300517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":20,"alternative-id":["S2213716519300517"],"URL":"https:\/\/doi.org\/10.1016\/j.jgar.2019.02.011","relation":{},"ISSN":["2213-7165"],"issn-type":[{"value":"2213-7165","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A real-time PCR assay for detecting a penA mutation associated with ceftriaxone resistance in Neisseria gonorrhoeae","name":"articletitle","label":"Article Title"},{"value":"Journal of Global Antimicrobial Resistance","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jgar.2019.02.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}