{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:31:44Z","timestamp":1791952304531,"version":"4.3.4"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T00:00:00Z","timestamp":1732147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012232","name":"National Key Clinical Specialty Discipline Construction Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012232","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Global Antimicrobial Resistance"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.jgar.2024.11.003","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:58:40Z","timestamp":1733187520000},"page":"72-80","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient"],"prefix":"10.1016","volume":"40","author":[{"given":"Yongjin","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahao","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengpeijie","family":"Dan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruilan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Yu","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.jgar.2024.11.003_bib0001","doi-asserted-by":"crossref","first-page":"e00001","DOI":"10.1128\/CMR.00001-19","article-title":"Hypervirulent Klebsiella pneumoniae","volume":"32","author":"Russo","year":"2019","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.jgar.2024.11.003_bib0002","doi-asserted-by":"crossref","first-page":"e141","DOI":"10.1093\/cid\/ciz1095","article-title":"An emerging clone, Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae sequence type 16, associated with high mortality rates in a CC258-endemic setting","volume":"71","author":"Andrey","year":"2020","journal-title":"Clin Infect Dis"},{"issue":"4","key":"10.1016\/j.jgar.2024.11.003_bib0003","doi-asserted-by":"crossref","first-page":"e02610","DOI":"10.1128\/AAC.02610-16","article-title":"Importance of Clonal Complex 258 and IncFK2-like Plasmids among a Global Collection of Klebsiella pneumoniae with blaKPC","volume":"61","author":"Peirano","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jgar.2024.11.003_bib0004","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ebiom.2017.04.032","article-title":"Nationwide surveillance of clinical carbapenem-resistant Enterobacteriaceae (CRE) strains in China","volume":"19","author":"Zhang","year":"2017","journal-title":"EBioMedicine"},{"key":"10.1016\/j.jgar.2024.11.003_bib0005","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S1473-3099(21)00399-6","article-title":"Clinical outcomes and bacterial characteristics of carbapenem-resistant Klebsiella pneumoniae complex among patients from different global regions (CRACKLE-2): a prospective, multicentre, cohort study","volume":"22","author":"Wang","year":"2022","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.jgar.2024.11.003_bib0006","doi-asserted-by":"crossref","first-page":"S196","DOI":"10.1093\/cid\/ciy660","article-title":"Phenotypic and genotypic characterization of carbapenem-resistant Enterobacteriaceae: data from a longitudinal large-scale CRE study in China (2012-2016)","volume":"67","author":"Wang","year":"2018","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jgar.2024.11.003_bib0007","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/s41579-021-00650-4","article-title":"Horizontal gene transfer and adaptive evolution in bacteria","volume":"20","author":"Arnold","year":"2022","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.jgar.2024.11.003_bib0008","doi-asserted-by":"crossref","first-page":"2226","DOI":"10.1016\/S0140-6736(22)01011-X","article-title":"The changing face of Klebsiella pneumoniae carbapenemase: in-vivo mutation in patient with chest infection","volume":"399","author":"Shen","year":"2022","journal-title":"Lancet"},{"key":"10.1016\/j.jgar.2024.11.003_bib0009","doi-asserted-by":"crossref","first-page":"106747","DOI":"10.1016\/j.ijantimicag.2023.106747","article-title":"Within-host resistance evolution of a fatal ST11 hypervirulent carbapenem-resistant Klebsiella pneumoniae","volume":"61","author":"Pu","year":"2023","journal-title":"Int J Antimicrob Agents"},{"key":"10.1016\/j.jgar.2024.11.003_bib0010","doi-asserted-by":"crossref","first-page":"S215","DOI":"10.1093\/infdis\/jiz586","article-title":"The predominance of strain replacement among Enterobacteriaceae pairs with emerging carbapenem resistance during hospitalization","volume":"221","author":"Ding","year":"2020","journal-title":"J Infect Dis"},{"key":"10.1016\/j.jgar.2024.11.003_bib0011","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1080\/22221751.2021.1937327","article-title":"Resistance evolution of hypervirulent carbapenem-resistant Klebsiella pneumoniae ST11 during treatment with tigecycline and polymyxin","volume":"10","author":"Jin","year":"2021","journal-title":"Emerg Microbes Infect"},{"key":"10.1016\/j.jgar.2024.11.003_bib0012","doi-asserted-by":"crossref","first-page":"7612","DOI":"10.1038\/srep07612","article-title":"MLST-based inference of genetic diversity and population structure of clinical Klebsiella pneumoniae, China","volume":"5","author":"Guo","year":"2015","journal-title":"Sci Rep"},{"key":"10.1016\/j.jgar.2024.11.003_bib0013","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1473-3099(17)30489-9","article-title":"A fatal outbreak of ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae in a Chinese hospital: a molecular epidemiological study","volume":"18","author":"Gu","year":"2018","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.jgar.2024.11.003_bib0014","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","article-title":"Multiplex PCR for detection of acquired carbapenemase genes","volume":"70","author":"Poirel","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.jgar.2024.11.003_bib0015","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1128\/aem.56.6.1875-1881.1990","article-title":"Modeling of the bacterial growth curve","volume":"56","author":"Zwietering","year":"1990","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.jgar.2024.11.003_bib0016","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13756-017-0200-5","article-title":"High burden of carbapenem-resistant Enterobacteriaceae (CRE) fecal carriage at a teaching hospital: cost-effectiveness of screening in low-resource setting","volume":"6","author":"Zaidah","year":"2017","journal-title":"Antimicrob Resist Infect Control"},{"key":"10.1016\/j.jgar.2024.11.003_bib0017","unstructured":"CLSI, Performance standards for antimicrobial susceptibility testing. 2023, Clinical and Laboratory Standards Institute: USA."},{"key":"10.1016\/j.jgar.2024.11.003_bib0018","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.3390\/ijerph10072720","article-title":"Optimization of pulse-field gel electrophoresis for subtyping of Klebsiella pneumoniae","volume":"10","author":"Han","year":"2013","journal-title":"Int J Environ Res Public Health"},{"issue":"1","key":"10.1016\/j.jgar.2024.11.003_bib0019","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s13073-021-00936-5","article-title":"Mobilization of the nonconjugative virulence plasmid from hypervirulent Klebsiella pneumoniae","volume":"13","author":"Xu","year":"2021","journal-title":"Genome Med"},{"issue":"1","key":"10.1016\/j.jgar.2024.11.003_bib0020","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1186\/s13073-023-01260-w","article-title":"Mobilizable plasmids drive the spread of antimicrobial resistance genes and virulence genes in Klebsiella pneumoniae","volume":"15","author":"Zhang","year":"2023","journal-title":"Genome Med"},{"key":"10.1016\/j.jgar.2024.11.003_bib0021","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1186\/1756-0500-5-337","article-title":"AdapterRemoval: easy cleaning of next-generation sequencing reads","volume":"5","author":"Lindgreen","year":"2012","journal-title":"BMC Res Notes"},{"key":"10.1016\/j.jgar.2024.11.003_bib0022","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J Comput Biol"},{"key":"10.1016\/j.jgar.2024.11.003_bib0023","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1101\/gr.215087.116","article-title":"Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation","volume":"27","author":"Koren","year":"2017","journal-title":"Genome Res"},{"key":"10.1016\/j.jgar.2024.11.003_bib0024","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"issue":"W1","key":"10.1016\/j.jgar.2024.11.003_bib0025","doi-asserted-by":"crossref","first-page":"W768","DOI":"10.1093\/nar\/gkac321","article-title":"VRprofile2: detection of antibiotic resistance-associated mobilome in bacterial pathogens","volume":"50","author":"Wang","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jgar.2024.11.003_bib0026","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1093\/bioinformatics\/bti054","article-title":"Circular genome visualization and exploration using CGView","volume":"21","author":"Stothard","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jgar.2024.11.003_bib0027","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1186\/1471-2164-12-402","article-title":"BLAST ring image generator (BRIG): simple prokaryote genome comparisons","volume":"12","author":"Alikhan","year":"2011","journal-title":"BMC Genomics"},{"key":"10.1016\/j.jgar.2024.11.003_bib0028","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: a genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jgar.2024.11.003_bib0029","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1128\/jcm.33.9.2233-2239.1995","article-title":"Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing","volume":"33","author":"Tenover","year":"1995","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jgar.2024.11.003_bib0030","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.2147\/IDR.S179618","article-title":"Emergence of tigecycline resistance in Escherichia coli co-producing MCR-1 and NDM-5 during tigecycline salvage treatment","volume":"11","author":"Wang","year":"2018","journal-title":"Infect Drug Resist"},{"key":"10.1016\/j.jgar.2024.11.003_bib0031","article-title":"Identification of Klebsiella capsule synthesis loci from whole genome data","volume":"2","author":"Wyres","year":"2016","journal-title":"Microb Genom"},{"issue":"3","key":"10.1016\/j.jgar.2024.11.003_bib0032","article-title":"Kaptive 2.0: updated capsule and lipopolysaccharide locus typing for the Klebsiella pneumoniae species complex","volume":"8","author":"Lam","year":"2022","journal-title":"Microb Genom"},{"key":"10.1016\/j.jgar.2024.11.003_bib0033","doi-asserted-by":"crossref","first-page":"D644","DOI":"10.1093\/nar\/gkaa821","article-title":"BacWGSTdb 2.0: a one-stop repository for bacterial whole-genome sequence typing and source tracking","volume":"49","author":"Feng","year":"2021","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"10.1016\/j.jgar.2024.11.003_bib0034","doi-asserted-by":"crossref","first-page":"e00583","DOI":"10.1128\/mBio.00583-18","article-title":"Extensive Gene Amplification as a Mechanism for Piperacillin-Tazobactam Resistance in Escherichia coli","volume":"9","author":"Schechter","year":"2018","journal-title":"mBio"},{"key":"10.1016\/j.jgar.2024.11.003_bib0035","doi-asserted-by":"crossref","first-page":"4915","DOI":"10.1038\/s41467-020-18668-2","article-title":"Piperacillin\/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of bla(TEM-1B)","volume":"11","author":"Hubbard","year":"2020","journal-title":"Nat Commun"},{"key":"10.1016\/j.jgar.2024.11.003_bib0036","doi-asserted-by":"crossref","first-page":"3179","DOI":"10.1093\/jac\/dkz349","article-title":"Resistance to piperacillin\/tazobactam in Escherichia coli resulting from extensive IS26-associated gene amplification of blaTEM-1","volume":"74","author":"Hansen","year":"2019","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.jgar.2024.11.003_bib0037","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4103\/jms.jms_25_17","article-title":"World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics","volume":"32","author":"Shrivastava","year":"2018","journal-title":"J Med Soc"},{"issue":"10325","key":"10.1016\/j.jgar.2024.11.003_bib0038","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","author":"Antimicrobial Resistance","year":"2022","journal-title":"Lancet"},{"key":"10.1016\/j.jgar.2024.11.003_bib0039","doi-asserted-by":"crossref","first-page":"S206","DOI":"10.1093\/infdis\/jiz622","article-title":"The colonization of carbapenem-resistant Klebsiella pneumoniae: epidemiology, resistance mechanisms, and risk factors in patients admitted to intensive care units in China","volume":"221","author":"Qin","year":"2020","journal-title":"J Infect Dis"},{"key":"10.1016\/j.jgar.2024.11.003_bib0040","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1093\/cid\/ciac268","article-title":"America 2022 guidance on the treatment of extended-spectrum \u03b2-lactamase producing Enterobacterales (ESBL-E), carbapenem-resistant Enterobacterales (CRE), and Pseudomonas aeruginosa with difficult-to-treat resistance (DTR-P. aeruginosa)","volume":"75","author":"Tamma","year":"2022","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jgar.2024.11.003_bib0041","doi-asserted-by":"crossref","first-page":"h6420","DOI":"10.1136\/bmj.h6420","article-title":"Antibiotic resistance in Enterobacteriaceae: mechanisms and clinical implications","author":"Iredell","year":"2016","journal-title":"Bmj"},{"key":"10.1016\/j.jgar.2024.11.003_bib0042","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/s41579-023-00877-3","article-title":"Bacterial defences: mechanisms, evolution and antimicrobial resistance","volume":"21","author":"Smith","year":"2023","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.jgar.2024.11.003_bib0043","first-page":"2087","article-title":"Concomitant carriage of KPC-producing and non-KPC-producing Klebsiella pneumoniae ST512 within a single patient","volume":"75","author":"Jousset","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.jgar.2024.11.003_bib0044","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1093\/femsre\/fuy031","article-title":"Strategies to combat antimicrobial resistance: anti-plasmid and plasmid curing","volume":"42","author":"Buckner","year":"2018","journal-title":"FEMS Microbiol Rev"}],"container-title":["Journal of Global Antimicrobial Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213716524004442?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2213716524004442?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T22:33:06Z","timestamp":1738881186000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2213716524004442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":44,"alternative-id":["S2213716524004442"],"URL":"https:\/\/doi.org\/10.1016\/j.jgar.2024.11.003","relation":{},"ISSN":["2213-7165"],"issn-type":[{"value":"2213-7165","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient","name":"articletitle","label":"Article Title"},{"value":"Journal of Global Antimicrobial Resistance","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jgar.2024.11.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.","name":"copyright","label":"Copyright"}]}}